Restriction map for
pF1KB6468
(Circular) (Six-base) MAPSORT of: pF1KB6468.seq Check: 3595 from: 1 to: 4175
pF1KB6468 4175 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 326 1213 326
Size: 887 3288
Acc65I G'GTAC_C
Cuts at: 1191 1191
Size: 4175
AclI AA'CG_TT
Cuts at: 4015 4015
Size: 4175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 510 2000 2432 3179 3447 470
Size: 40 1490 432 747 268 1198
Fragments arranged by size:
1490 1198 747 432 268 40
AflIII A'CryG_T
Cuts at: 1527 2631 3608 1527
Size: 1104 977 2094
Fragments arranged by size:
2094 1104 977
AgeI A'CCGG_T
Cuts at: 2563 2563
Size: 4175
AleI CACnn'nnGTG
Cuts at: 253 253
Size: 4175
AlwNI CAG_nnn'CTG
Cuts at: 1480 3047 1480
Size: 1567 2608
ApaLI G'TGCA_C
Cuts at: 2945 2945
Size: 4175
ApoI r'AATT_y
Cuts at: 1179 3528 3784 1179
Size: 2349 256 1570
Fragments arranged by size:
2349 1570 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4175
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
AvaI C'yCGr_G
Cuts at: 1195 1195
Size: 4175
BamHI G'GATC_C
Cuts at: 1200 1200
Size: 4175
BanI G'GyrC_C
Cuts at: 1191 1605 1853 1888 1191
Size: 414 248 35 3478
Fragments arranged by size:
3478 414 248 35
BanII G_rGCy'C
Cuts at: 1189 2219 1189
Size: 1030 3145
BbeI G_GCGC'C
Cuts at: 1609 1857 1609
Size: 248 3927
BbsI GAAGACnn'nnnn_
Cuts at: 137 652 137
Size: 515 3660
BbvCI CC'TCA_GC
Cuts at: 812 812
Size: 4175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 144 178 1083 1117 144
Size: 34 905 34 3202
Fragments arranged by size:
3202 905 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2073 2834 4175 2073
Size: 761 1341 2073
Fragments arranged by size:
2073 1341 761
BclI T'GATC_A
Cuts at: 1695 1695
Size: 4175
BfrBI ATG'CAT
Cuts at: 837 837
Size: 4175
BglII A'GATC_T
Cuts at: 1032 1061 1690 3727 1032
Size: 29 629 2037 1480
Fragments arranged by size:
2037 1480 629 29
BlpI GC'TnA_GC
Cuts at: 1287 1287
Size: 4175
Bme1580I G_kGCm'C
Cuts at: 1800 1893 2949 1800
Size: 93 1056 3026
Fragments arranged by size:
3026 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 591 1387 1468 1803 3947 591
Size: 796 81 335 2144 819
Fragments arranged by size:
2144 819 796 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 4175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 452 251
Size: 201 3974
Bpu10I CC'TnA_GC
Cuts at: 812 812
Size: 4175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1351 2230 2722 3020 1351
Size: 879 492 298 2506
Fragments arranged by size:
2506 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3879 3879
Size: 4175
BsaAI yAC'GTr
Cuts at: 1528 1528
Size: 4175
BsaBI GATnn'nnATC
Cuts at: 6 268 1689 3726 6
Size: 262 1421 2037 455
Fragments arranged by size:
2037 1421 455 262
BsaHI Gr'CG_yC
Cuts at: 1606 1854 2556 4052 1606
Size: 248 702 1496 1729
Fragments arranged by size:
1729 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1364 1885 2563 2837 2984 3395 1364
Size: 521 678 274 147 411 2144
Fragments arranged by size:
2144 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 835 960 835
Size: 125 4050
BseYI C'CCAG_C
Cuts at: 1600 2358 2935 3431 1600
Size: 758 577 496 2344
Fragments arranged by size:
2344 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 99 1123 99
Size: 1024 3151
BsiEI CG_ry'CG
Cuts at: 104 168 1717 1763 2971 104
Size: 64 1549 46 1208 1308
Fragments arranged by size:
1549 1308 1208 64 46
BsiHKAI G_wGCw'C
Cuts at: 1189 1967 2157 2949 1189
Size: 778 190 792 2415
Fragments arranged by size:
2415 792 778 190
BsmI GAATG_Cn'
Cuts at: 839 839
Size: 4175
Bsp1286I G_dGCh'C
Cuts at: 1189 1800 1893 1967 2157 2219 2949 1189
Size: 611 93 74 190 62 730 2415
Fragments arranged by size:
2415 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3395 3395
Size: 4175
BspHI T'CATG_A
Cuts at: 276 461 3351 276
Size: 185 2890 1100
Fragments arranged by size:
2890 1100 185
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1225 1439 1741 2122 1225
Size: 214 302 381 3278
Fragments arranged by size:
3278 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2467 2521 4005 4170 50
Size: 2417 54 1484 165 55
Fragments arranged by size:
2417 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 350 2087 350
Size: 1737 2438
BsrFI r'CCGG_y
Cuts at: 2173 2354 2563 2173
Size: 181 209 3785
Fragments arranged by size:
3785 209 181
BssSI C'ACGA_G
Cuts at: 2446 2804 2446
Size: 358 3817
BstAPI GCAn_nnn'nTGC
Cuts at: 415 1552 415
Size: 1137 3038
BstBI TT'CG_AA
Cuts at: 2536 2536
Size: 4175
BstYI r'GATC_y
Cuts at: 1 532 1032 1061 1200 1667 1690 2025 2271
Size: 531 500 29 139 467 23 335 246
Cuts at: 2271 3283 3369 3381 3727 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 531 500 467 449 346 335 246
139 86 29 23 12
BtgI C'CryG_G
Cuts at: 107 368 107
Size: 261 3914
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2125 2306 2469 2125
Size: 181 163 3831
Fragments arranged by size:
3831 181 163
BtsI GCAGTG_nn'
Cuts at: 149 450 1418 149
Size: 301 968 2906
Fragments arranged by size:
2906 968 301
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 639 674 639
Size: 35 4140
DraI TTT'AAA
Cuts at: 1174 1174
Size: 4175
DrdI GACnn_nn'nnGTC
Cuts at: 1881 2739 1881
Size: 858 3317
EaeI y'GGCC_r
Cuts at: 197 714 1760 1934 2325 2352 3697 197
Size: 517 1046 174 391 27 1345 675
Fragments arranged by size:
1345 1046 675 517 391 174 27
EagI C'GGCC_G
Cuts at: 1760 1760
Size: 4175
EarI CTCTTCn'nnn_
Cuts at: 910 992 2198 2408 910
Size: 82 1206 210 2677
Fragments arranged by size:
2677 1206 210 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2383 2693 2839 3985 2383
Size: 310 146 1146 2573
Fragments arranged by size:
2573 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1187 1187
Size: 4175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 452 470 510 2000 2432 3179 3447 251
Size: 201 18 40 1490 432 747 268 979
Fragments arranged by size:
1490 979 747 432 268 201 40 18
EcoNI CCTnn'n_nnAGG
Cuts at: 928 928
Size: 4175
EcoO109I rG'GnC_Cy
Cuts at: 1314 1314
Size: 4175
EcoRI G'AATT_C
Cuts at: 1179 1179
Size: 4175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 405 646 2194 405
Size: 241 1548 2386
Fragments arranged by size:
2386 1548 241
FspI TGC'GCA
Cuts at: 1956 1956
Size: 4175
HaeII r_GCGC'y
Cuts at: 128 1609 1857 2879 3858 128
Size: 1481 248 1022 979 445
Fragments arranged by size:
1481 1022 979 445 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 577 609 1053 1085 2017 2047 2049 2079 577
Size: 32 444 32 932 30 2 30 2673
Fragments arranged by size:
2673 932 444 32 32 30 30 2
HincII GTy'rAC
Cuts at: 956 1214 956
Size: 258 3917
HindIII A'AGCT_T
Cuts at: 518 518
Size: 4175
HpaI GTT'AAC
Cuts at: 956 956
Size: 4175
KasI G'GCGC_C
Cuts at: 1605 1853 1605
Size: 248 3927
KpnI G_GTAC'C
Cuts at: 1195 1195
Size: 4175
MluI A'CGCG_T
Cuts at: 3608 3608
Size: 4175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 637 2846 3030 637
Size: 2209 184 1782
Fragments arranged by size:
2209 1782 184
MscI TGG'CCA
Cuts at: 716 1936 716
Size: 1220 2955
MslI CAynn'nnrTG
Cuts at: 253 281 2164 2291 253
Size: 28 1883 127 2137
Fragments arranged by size:
2137 1883 127 28
MspA1I CmG'CkG
Cuts at: 1006 1286 1600 1960 2973 3218 1006
Size: 280 314 360 1013 245 1963
Fragments arranged by size:
1963 1013 360 314 280 245
NaeI GCC'GGC
Cuts at: 2356 2356
Size: 4175
NarI GG'CG_CC
Cuts at: 1606 1854 1606
Size: 248 3927
NcoI C'CATG_G
Cuts at: 107 368 107
Size: 261 3914
NdeI CA'TA_TG
Cuts at: 993 993
Size: 4175
NgoMIV G'CCGG_C
Cuts at: 2354 2354
Size: 4175
NsiI A_TGCA'T
Cuts at: 839 839
Size: 4175
NspI r_CATG'y
Cuts at: 1228 1728 2635 1228
Size: 500 907 2768
Fragments arranged by size:
2768 907 500
PciI A'CATG_T
Cuts at: 2631 2631
Size: 4175
PmeI GTTT'AAAC
Cuts at: 1174 1174
Size: 4175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 4143
PstI C_TGCA'G
Cuts at: 1222 1907 1222
Size: 685 3490
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4175
PvuII CAG'CTG
Cuts at: 1006 1600 1960 1006
Size: 594 360 3221
Fragments arranged by size:
3221 594 360
RsrII CG'GwC_CG
Cuts at: 2370 2370
Size: 4175
SacI G_AGCT'C
Cuts at: 1189 1189
Size: 4175
SalI G'TCGA_C
Cuts at: 1212 1212
Size: 4175
SapI GCTCTTCn'nnn_
Cuts at: 2198 2408 2198
Size: 210 3965
SbfI CC_TGCA'GG
Cuts at: 1222 1222
Size: 4175
ScaI AGT'ACT
Cuts at: 3692 3692
Size: 4175
SfcI C'TryA_G
Cuts at: 33 327 1218 1903 2896 3087 33
Size: 294 891 685 993 191 1121
Fragments arranged by size:
1121 993 891 685 294 191
SfoI GGC'GCC
Cuts at: 1607 1855 1607
Size: 248 3927
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
SmaI CCC'GGG
Cuts at: 1197 1197
Size: 4175
SmlI C'TyrA_G
Cuts at: 1330 2245 2737 2999 1330
Size: 915 492 262 2506
Fragments arranged by size:
2506 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4175
SphI G_CATG'C
Cuts at: 1228 1728 1228
Size: 500 3675
StyI C'CwwG_G
Cuts at: 107 368 1055 1309 1663 107
Size: 261 687 254 354 2619
Fragments arranged by size:
2619 687 354 261 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1758 1891 2350 2559 1758
Size: 133 459 209 3374
Fragments arranged by size:
3374 459 209 133
TatI w'GTAC_w
Cuts at: 3690 3690
Size: 4175
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3692 3724 3692
Size: 32 4143
Tth111I GACn'n_nGTC
Cuts at: 1972 1972
Size: 4175
XbaI T'CTAG_A
Cuts at: 1206 1206
Size: 4175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1670 1670
Size: 4175
XmaI C'CCGG_G
Cuts at: 1195 1195
Size: 4175
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BglI BmgBI BmtI BsaXI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
DraIII EcoRV FseI FspAI MfeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI