Restriction map for
pF1KB6465
(Circular) (Six-base) MAPSORT of: pF1KB6465.seq Check: 6461 from: 1 to: 4079
pF1KB6465 4079 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 1117 1117
Size: 4079
Acc65I G'GTAC_C
Cuts at: 1095 1095
Size: 4079
AclI AA'CG_TT
Cuts at: 3919 3919
Size: 4079
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 1904 2336 3083 3351 440
Size: 1464 432 747 268 1168
Fragments arranged by size:
1464 1168 747 432 268
AflIII A'CryG_T
Cuts at: 1431 2535 3512 1431
Size: 1104 977 1998
Fragments arranged by size:
1998 1104 977
AgeI A'CCGG_T
Cuts at: 2467 2467
Size: 4079
AhdI GACnn_n'nnGTC
Cuts at: 612 612
Size: 4079
AlwNI CAG_nnn'CTG
Cuts at: 1384 2951 1384
Size: 1567 2512
ApaLI G'TGCA_C
Cuts at: 2849 2849
Size: 4079
ApoI r'AATT_y
Cuts at: 1083 3432 3688 1083
Size: 2349 256 1474
Fragments arranged by size:
2349 1474 256
AseI AT'TA_AT
Cuts at: 20 305 851 950 20
Size: 285 546 99 3149
Fragments arranged by size:
3149 546 285 99
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
AvaI C'yCGr_G
Cuts at: 1099 1099
Size: 4079
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 221 254 221
Size: 33 4046
BamHI G'GATC_C
Cuts at: 1104 1104
Size: 4079
BanI G'GyrC_C
Cuts at: 1095 1509 1757 1792 1095
Size: 414 248 35 3382
Fragments arranged by size:
3382 414 248 35
BanII G_rGCy'C
Cuts at: 1093 2123 1093
Size: 1030 3049
BbeI G_GCGC'C
Cuts at: 1513 1761 1513
Size: 248 3831
BbsI GAAGACnn'nnnn_
Cuts at: 608 1022 608
Size: 414 3665
BciVI GTATCCnnnnn_n'
Cuts at: 1977 2738 4079 1977
Size: 761 1341 1977
Fragments arranged by size:
1977 1341 761
BclI T'GATC_A
Cuts at: 321 1599 321
Size: 1278 2801
BglII A'GATC_T
Cuts at: 259 1594 3631 259
Size: 1335 2037 707
Fragments arranged by size:
2037 1335 707
BlpI GC'TnA_GC
Cuts at: 1191 1191
Size: 4079
Bme1580I G_kGCm'C
Cuts at: 1704 1797 2853 1704
Size: 93 1056 2930
Fragments arranged by size:
2930 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 242 1291 1372 1707 3851 242
Size: 1049 81 335 2144 470
Fragments arranged by size:
2144 1049 470 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2446 2446
Size: 4079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1255 2134 2626 2924 1255
Size: 879 492 298 2410
Fragments arranged by size:
2410 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3783 3783
Size: 4079
BsaAI yAC'GTr
Cuts at: 1432 1432
Size: 4079
BsaBI GATnn'nnATC
Cuts at: 6 326 1593 3630 6
Size: 320 1267 2037 455
Fragments arranged by size:
2037 1267 455 320
BsaHI Gr'CG_yC
Cuts at: 1510 1758 2460 3956 1510
Size: 248 702 1496 1633
Fragments arranged by size:
1633 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1268 1789 2467 2741 2888 3299 1268
Size: 521 678 274 147 411 2048
Fragments arranged by size:
2048 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 778 778
Size: 4079
BseYI C'CCAG_C
Cuts at: 1504 2262 2839 3335 1504
Size: 758 577 496 2248
Fragments arranged by size:
2248 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 1025 562
Size: 463 3616
BsiEI CG_ry'CG
Cuts at: 104 1621 1667 2875 104
Size: 1517 46 1208 1308
Fragments arranged by size:
1517 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1093 1871 2061 2853 1093
Size: 778 190 792 2319
Fragments arranged by size:
2319 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1093 1704 1797 1871 2061 2123 2853 1093
Size: 611 93 74 190 62 730 2319
Fragments arranged by size:
2319 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3299 3299
Size: 4079
BspHI T'CATG_A
Cuts at: 3255 3255
Size: 4079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1129 1343 1645 2026 1129
Size: 214 302 381 3182
Fragments arranged by size:
3182 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2371 2425 3909 4074 50
Size: 2321 54 1484 165 55
Fragments arranged by size:
2321 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1991 1991
Size: 4079
BsrFI r'CCGG_y
Cuts at: 2077 2258 2467 2077
Size: 181 209 3689
Fragments arranged by size:
3689 209 181
BssSI C'ACGA_G
Cuts at: 2350 2708 2350
Size: 358 3721
BstAPI GCAn_nnn'nTGC
Cuts at: 922 1456 922
Size: 534 3545
BstBI TT'CG_AA
Cuts at: 2440 2440
Size: 4079
BstYI r'GATC_y
Cuts at: 1 259 513 1104 1571 1594 1929 2175 3187
Size: 258 254 591 467 23 335 246 1012
Cuts at: 3187 3273 3285 3631 1
Size: 86 12 346 449
Fragments arranged by size:
1012 591 467 449 346 335 258 254
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 713 713
Size: 4079
BtgI C'CryG_G
Cuts at: 107 739 107
Size: 632 3447
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2029 2210 2373 2029
Size: 181 163 3735
Fragments arranged by size:
3735 181 163
BtsI GCAGTG_nn'
Cuts at: 1322 1322
Size: 4079
ClaI AT'CG_AT
Cuts at: 838 838
Size: 4079
DraI TTT'AAA
Cuts at: 539 1078 539
Size: 539 3540
DrdI GACnn_nn'nnGTC
Cuts at: 1785 2643 1785
Size: 858 3221
EaeI y'GGCC_r
Cuts at: 1664 1838 2229 2256 3601 1664
Size: 174 391 27 1345 2142
Fragments arranged by size:
2142 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1664 1664
Size: 4079
EarI CTCTTCn'nnn_
Cuts at: 1000 2102 2312 1000
Size: 1102 210 2767
Fragments arranged by size:
2767 1102 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2287 2597 2743 3889 2287
Size: 310 146 1146 2477
Fragments arranged by size:
2477 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1091 1091
Size: 4079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 1904 2336 3083 3351 440
Size: 1464 432 747 268 1168
Fragments arranged by size:
1464 1168 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1218 1218
Size: 4079
EcoRI G'AATT_C
Cuts at: 1083 1083
Size: 4079
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 240 2098 240
Size: 1858 2221
FspI TGC'GCA
Cuts at: 1860 1860
Size: 4079
HaeII r_GCGC'y
Cuts at: 1513 1761 2783 3762 1513
Size: 248 1022 979 1830
Fragments arranged by size:
1830 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 598 630 1921 1951 1953 1983 598
Size: 32 1291 30 2 30 2694
Fragments arranged by size:
2694 1291 32 30 30 2
HincII GTy'rAC
Cuts at: 1118 1118
Size: 4079
HindIII A'AGCT_T
Cuts at: 217 217
Size: 4079
KasI G'GCGC_C
Cuts at: 1509 1757 1509
Size: 248 3831
KpnI G_GTAC'C
Cuts at: 1099 1099
Size: 4079
MluI A'CGCG_T
Cuts at: 3512 3512
Size: 4079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 205 2750 2934 205
Size: 2545 184 1350
Fragments arranged by size:
2545 1350 184
MscI TGG'CCA
Cuts at: 1840 1840
Size: 4079
MslI CAynn'nnrTG
Cuts at: 2068 2195 2068
Size: 127 3952
MspA1I CmG'CkG
Cuts at: 1190 1504 1864 2877 3122 1190
Size: 314 360 1013 245 2147
Fragments arranged by size:
2147 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2260 2260
Size: 4079
NarI GG'CG_CC
Cuts at: 1510 1758 1510
Size: 248 3831
NcoI C'CATG_G
Cuts at: 107 739 107
Size: 632 3447
NgoMIV G'CCGG_C
Cuts at: 2258 2258
Size: 4079
NspI r_CATG'y
Cuts at: 1132 1632 2539 1132
Size: 500 907 2672
Fragments arranged by size:
2672 907 500
PciI A'CATG_T
Cuts at: 2535 2535
Size: 4079
PmeI GTTT'AAAC
Cuts at: 1078 1078
Size: 4079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3218 3250 3218
Size: 32 4047
PstI C_TGCA'G
Cuts at: 1126 1811 1126
Size: 685 3394
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4079
PvuII CAG'CTG
Cuts at: 1504 1864 1504
Size: 360 3719
RsrII CG'GwC_CG
Cuts at: 2274 2274
Size: 4079
SacI G_AGCT'C
Cuts at: 1093 1093
Size: 4079
SalI G'TCGA_C
Cuts at: 1116 1116
Size: 4079
SapI GCTCTTCn'nnn_
Cuts at: 2102 2312 2102
Size: 210 3869
SbfI CC_TGCA'GG
Cuts at: 1126 1126
Size: 4079
ScaI AGT'ACT
Cuts at: 3596 3596
Size: 4079
SfcI C'TryA_G
Cuts at: 33 1122 1807 2800 2991 33
Size: 1089 685 993 191 1121
Fragments arranged by size:
1121 1089 993 685 191
SfoI GGC'GCC
Cuts at: 1511 1759 1511
Size: 248 3831
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
SmaI CCC'GGG
Cuts at: 1101 1101
Size: 4079
SmlI C'TyrA_G
Cuts at: 1234 2149 2641 2903 1234
Size: 915 492 262 2410
Fragments arranged by size:
2410 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4079
SphI G_CATG'C
Cuts at: 1132 1632 1132
Size: 500 3579
StyI C'CwwG_G
Cuts at: 107 286 739 1213 1567 107
Size: 179 453 474 354 2619
Fragments arranged by size:
2619 474 453 354 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1662 1795 2254 2463 1662
Size: 133 459 209 3278
Fragments arranged by size:
3278 459 209 133
TatI w'GTAC_w
Cuts at: 3594 3594
Size: 4079
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3596 3628 3596
Size: 32 4047
Tth111I GACn'n_nGTC
Cuts at: 1876 1876
Size: 4079
XbaI T'CTAG_A
Cuts at: 1110 1110
Size: 4079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 756 1574 756
Size: 818 3261
XmaI C'CCGG_G
Cuts at: 1099 1099
Size: 4079
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI