Restriction map for
pF1KB6464
(Circular) (Six-base) MAPSORT of: pF1KB6464.seq Check: 4691 from: 1 to: 4169
pF1KB6464 4169 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 271 271
Size: 4169
AatII G_ACGT'C
Cuts at: 391 391
Size: 4169
AccI GT'mk_AC
Cuts at: 1207 1207
Size: 4169
Acc65I G'GTAC_C
Cuts at: 1185 1185
Size: 4169
AclI AA'CG_TT
Cuts at: 1163 4009 1163
Size: 2846 1323
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 585 794 1994 2426 3173 3441 295
Size: 290 209 1200 432 747 268 1023
Fragments arranged by size:
1200 1023 747 432 290 268 209
AfeI AGC'GCT
Cuts at: 162 162
Size: 4169
AflIII A'CryG_T
Cuts at: 1521 2625 3602 1521
Size: 1104 977 2088
Fragments arranged by size:
2088 1104 977
AgeI A'CCGG_T
Cuts at: 2557 2557
Size: 4169
AleI CACnn'nnGTG
Cuts at: 310 310
Size: 4169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 328 328
Size: 4169
AlwNI CAG_nnn'CTG
Cuts at: 415 1474 3041 415
Size: 1059 1567 1543
Fragments arranged by size:
1567 1543 1059
ApaI G_GGCC'C
Cuts at: 126 991 126
Size: 865 3304
ApaLI G'TGCA_C
Cuts at: 2939 2939
Size: 4169
ApoI r'AATT_y
Cuts at: 517 1173 3522 3778 517
Size: 656 2349 256 908
Fragments arranged by size:
2349 908 656 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4169
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
AvaI C'yCGr_G
Cuts at: 118 708 1189 118
Size: 590 481 3098
Fragments arranged by size:
3098 590 481
BamHI G'GATC_C
Cuts at: 1194 1194
Size: 4169
BanI G'GyrC_C
Cuts at: 567 1185 1599 1847 1882 567
Size: 618 414 248 35 2854
Fragments arranged by size:
2854 618 414 248 35
BanII G_rGCy'C
Cuts at: 126 698 991 1183 2213 126
Size: 572 293 192 1030 2082
Fragments arranged by size:
2082 1030 572 293 192
BbeI G_GCGC'C
Cuts at: 571 1603 1851 571
Size: 1032 248 2889
Fragments arranged by size:
2889 1032 248
BbvCI CC'TCA_GC
Cuts at: 577 577
Size: 4169
BciVI GTATCCnnnnn_n'
Cuts at: 2067 2828 4169 2067
Size: 761 1341 2067
Fragments arranged by size:
2067 1341 761
BclI T'GATC_A
Cuts at: 1689 1689
Size: 4169
BglI GCCn_nnn'nGGC
Cuts at: 151 806 151
Size: 655 3514
BglII A'GATC_T
Cuts at: 1684 3721 1684
Size: 2037 2132
BlpI GC'TnA_GC
Cuts at: 139 1281 139
Size: 1142 3027
Bme1580I G_kGCm'C
Cuts at: 126 991 1794 1887 2943 126
Size: 865 803 93 1056 1352
Fragments arranged by size:
1352 1056 865 803 93
BmrI ACTGGGnnnn_n'
Cuts at: 1381 1462 1797 3941 1381
Size: 81 335 2144 1609
Fragments arranged by size:
2144 1609 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 602 2536 602
Size: 1934 2235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 413
Size: 4169
Bpu10I CC'TnA_GC
Cuts at: 577 577
Size: 4169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1345 2224 2716 3014 1345
Size: 879 492 298 2500
Fragments arranged by size:
2500 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3873 3873
Size: 4169
BsaAI yAC'GTr
Cuts at: 1522 1522
Size: 4169
BsaBI GATnn'nnATC
Cuts at: 6 1683 3720 6
Size: 1677 2037 455
Fragments arranged by size:
2037 1677 455
BsaHI Gr'CG_yC
Cuts at: 388 568 1600 1848 2550 4046 388
Size: 180 1032 248 702 1496 511
Fragments arranged by size:
1496 1032 702 511 248 180
BsaWI w'CCGG_w
Cuts at: 716 1358 1879 2557 2831 2978 3389 716
Size: 642 521 678 274 147 411 1496
Fragments arranged by size:
1496 678 642 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 397 1156 126
Size: 271 759 3139
Fragments arranged by size:
3139 759 271
BseYI C'CCAG_C
Cuts at: 315 804 1594 2352 2929 3425 315
Size: 489 790 758 577 496 1059
Fragments arranged by size:
1059 790 758 577 496 489
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 1125 220
Size: 905 3264
BsiEI CG_ry'CG
Cuts at: 104 818 1711 1757 2965 104
Size: 714 893 46 1208 1308
Fragments arranged by size:
1308 1208 893 714 46
BsiHKAI G_wGCw'C
Cuts at: 307 1183 1961 2151 2943 307
Size: 876 778 190 792 1533
Fragments arranged by size:
1533 876 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 379 379
Size: 4169
Bsp1286I G_dGCh'C
Cuts at: 126 307 698 991 1183 1794 1887 1961 2151
Size: 181 391 293 192 611 93 74 190
Cuts at: 2151 2213 2943 126
Size: 62 730 1352
Fragments arranged by size:
1352 730 611 391 293 192 190 181
93 74 62
BspEI T'CCGG_A
Cuts at: 716 3389 716
Size: 2673 1496
BspHI T'CATG_A
Cuts at: 3345 3345
Size: 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 271 833 955 1219 1433 1735 2116 271
Size: 562 122 264 214 302 381 2324
Fragments arranged by size:
2324 562 381 302 264 214 122
BsrBI CCG'CTC
Cuts at: 50 2461 2515 3999 4164 50
Size: 2411 54 1484 165 55
Fragments arranged by size:
2411 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2081 2081
Size: 4169
BsrFI r'CCGG_y
Cuts at: 817 2167 2348 2557 817
Size: 1350 181 209 2429
Fragments arranged by size:
2429 1350 209 181
BssSI C'ACGA_G
Cuts at: 2440 2798 2440
Size: 358 3811
BstAPI GCAn_nnn'nTGC
Cuts at: 1024 1546 1024
Size: 522 3647
BstBI TT'CG_AA
Cuts at: 2530 2530
Size: 4169
BstYI r'GATC_y
Cuts at: 1 224 1194 1661 1684 2019 2265 3277 3363
Size: 223 970 467 23 335 246 1012 86
Cuts at: 3363 3375 3721 1
Size: 12 346 449
Fragments arranged by size:
1012 970 467 449 346 335 246 223
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 632 632
Size: 4169
BtgI C'CryG_G
Cuts at: 458 650 866 458
Size: 192 216 3761
Fragments arranged by size:
3761 216 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2119 2300 2463 2119
Size: 181 163 3825
Fragments arranged by size:
3825 181 163
BtsI GCAGTG_nn'
Cuts at: 1412 1412
Size: 4169
DraI TTT'AAA
Cuts at: 1168 1168
Size: 4169
DrdI GACnn_nn'nnGTC
Cuts at: 1875 2733 1875
Size: 858 3311
EaeI y'GGCC_r
Cuts at: 248 815 1754 1928 2319 2346 3691 248
Size: 567 939 174 391 27 1345 726
Fragments arranged by size:
1345 939 726 567 391 174 27
EagI C'GGCC_G
Cuts at: 815 1754 815
Size: 939 3230
EarI CTCTTCn'nnn_
Cuts at: 734 1054 2192 2402 734
Size: 320 1138 210 2501
Fragments arranged by size:
2501 1138 320 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 352 381 2377 2687 2833 3979 352
Size: 29 1996 310 146 1146 542
Fragments arranged by size:
1996 1146 542 310 146 29
EcoICRI GAG'CTC
Cuts at: 1181 1181
Size: 4169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 413 585 794 1994 2426 3173 3441 295
Size: 118 172 209 1200 432 747 268 1023
Fragments arranged by size:
1200 1023 747 432 268 209 172 118
EcoO109I rG'GnC_Cy
Cuts at: 122 123 987 988 1308 122
Size: 1 864 1 320 2983
Fragments arranged by size:
2983 864 320 1 1
EcoRI G'AATT_C
Cuts at: 1173 1173
Size: 4169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2188
Size: 4169
FspI TGC'GCA
Cuts at: 1950 1950
Size: 4169
HaeII r_GCGC'y
Cuts at: 146 164 329 571 1603 1851 2873 3852 146
Size: 18 165 242 1032 248 1022 979 463
Fragments arranged by size:
1032 1022 979 463 248 242 165 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 342 373 374 405 733 765 989 1021 2011
Size: 31 1 31 328 32 224 32 990
Cuts at: 2011 2041 2043 2073 342
Size: 30 2 30 2438
Fragments arranged by size:
2438 990 328 224 32 32 31 31
30 30 2 1
HincII GTy'rAC
Cuts at: 1208 1208
Size: 4169
KasI G'GCGC_C
Cuts at: 567 1599 1847 567
Size: 1032 248 2889
Fragments arranged by size:
2889 1032 248
KpnI G_GTAC'C
Cuts at: 1189 1189
Size: 4169
MluI A'CGCG_T
Cuts at: 3602 3602
Size: 4169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1184 2840 3024 1184
Size: 1656 184 2329
Fragments arranged by size:
2329 1656 184
MscI TGG'CCA
Cuts at: 250 1930 250
Size: 1680 2489
MslI CAynn'nnrTG
Cuts at: 196 310 457 832 2158 2285 196
Size: 114 147 375 1326 127 2080
Fragments arranged by size:
2080 1326 375 147 127 114
MspA1I CmG'CkG
Cuts at: 576 804 1280 1594 1954 2967 3212 576
Size: 228 476 314 360 1013 245 1533
Fragments arranged by size:
1533 1013 476 360 314 245 228
NaeI GCC'GGC
Cuts at: 2350 2350
Size: 4169
NarI GG'CG_CC
Cuts at: 568 1600 1848 568
Size: 1032 248 2889
Fragments arranged by size:
2889 1032 248
NcoI C'CATG_G
Cuts at: 458 650 866 458
Size: 192 216 3761
Fragments arranged by size:
3761 216 192
NgoMIV G'CCGG_C
Cuts at: 2348 2348
Size: 4169
NspI r_CATG'y
Cuts at: 1222 1722 2629 1222
Size: 500 907 2762
Fragments arranged by size:
2762 907 500
PasI CC'CwG_GG
Cuts at: 615 676 615
Size: 61 4108
PciI A'CATG_T
Cuts at: 2625 2625
Size: 4169
PflMI CCAn_nnn'nTGG
Cuts at: 621 621
Size: 4169
PmeI GTTT'AAAC
Cuts at: 1168 1168
Size: 4169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 4137
PspOMI G'GGCC_C
Cuts at: 122 987 122
Size: 865 3304
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1072 1104 1072
Size: 32 4137
PstI C_TGCA'G
Cuts at: 596 944 1216 1901 596
Size: 348 272 685 2864
Fragments arranged by size:
2864 685 348 272
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4169
PvuII CAG'CTG
Cuts at: 576 804 1594 1954 576
Size: 228 790 360 2791
Fragments arranged by size:
2791 790 360 228
RsrII CG'GwC_CG
Cuts at: 2364 2364
Size: 4169
SacI G_AGCT'C
Cuts at: 1183 1183
Size: 4169
SalI G'TCGA_C
Cuts at: 1206 1206
Size: 4169
SapI GCTCTTCn'nnn_
Cuts at: 2192 2402 2192
Size: 210 3959
SbfI CC_TGCA'GG
Cuts at: 596 1216 596
Size: 620 3549
ScaI AGT'ACT
Cuts at: 1114 3686 1114
Size: 2572 1597
SfcI C'TryA_G
Cuts at: 33 329 592 940 1212 1897 2890 3081 33
Size: 296 263 348 272 685 993 191 1121
Fragments arranged by size:
1121 993 685 348 296 272 263 191
SfoI GGC'GCC
Cuts at: 569 1601 1849 569
Size: 1032 248 2889
Fragments arranged by size:
2889 1032 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
SmaI CCC'GGG
Cuts at: 1191 1191
Size: 4169
SmlI C'TyrA_G
Cuts at: 1324 2239 2731 2993 1324
Size: 915 492 262 2500
Fragments arranged by size:
2500 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4169
SphI G_CATG'C
Cuts at: 1222 1722 1222
Size: 500 3669
StyI C'CwwG_G
Cuts at: 458 650 866 1303 1657 458
Size: 192 216 437 354 2970
Fragments arranged by size:
2970 437 354 216 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 674 1752 1885 2344 2553 674
Size: 1078 133 459 209 2290
Fragments arranged by size:
2290 1078 459 209 133
TatI w'GTAC_w
Cuts at: 1112 3684 1112
Size: 2572 1597
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 4137
Tth111I GACn'n_nGTC
Cuts at: 1966 1966
Size: 4169
XbaI T'CTAG_A
Cuts at: 1200 1200
Size: 4169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 648 1664 648
Size: 1016 3153
XmaI C'CCGG_G
Cuts at: 1189 1189
Size: 4169
XmnI GAAnn'nnTTC
Cuts at: 1084 1084
Size: 4169
ZraI GAC'GTC
Cuts at: 389 389
Size: 4169
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI AvrII BaeI BbsI BcgI
BfrBI BmgBI BmtI BsaXI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PfoI PmlI PpuMI PshAI PsiI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI