Restriction map for
pF1KB6462
(Circular) (Six-base) MAPSORT of: pF1KB6462.seq Check: 1776 from: 1 to: 4169
pF1KB6462 4169 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 597 1207 597
Size: 610 3559
Acc65I G'GTAC_C
Cuts at: 1185 1185
Size: 4169
AclI AA'CG_TT
Cuts at: 4009 4009
Size: 4169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 520 947 1994 2426 3173 3441 200
Size: 320 427 1047 432 747 268 928
Fragments arranged by size:
1047 928 747 432 427 320 268
AflIII A'CryG_T
Cuts at: 1521 2625 3602 1521
Size: 1104 977 2088
Fragments arranged by size:
2088 1104 977
AgeI A'CCGG_T
Cuts at: 2557 2557
Size: 4169
AlwNI CAG_nnn'CTG
Cuts at: 804 1474 3041 804
Size: 670 1567 1932
Fragments arranged by size:
1932 1567 670
ApaLI G'TGCA_C
Cuts at: 2939 2939
Size: 4169
ApoI r'AATT_y
Cuts at: 403 566 673 1142 1173 3522 3778 403
Size: 163 107 469 31 2349 256 794
Fragments arranged by size:
2349 794 469 256 163 107 31
AseI AT'TA_AT
Cuts at: 20 20
Size: 4169
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
AvaI C'yCGr_G
Cuts at: 1189 1189
Size: 4169
BamHI G'GATC_C
Cuts at: 1194 1194
Size: 4169
BanI G'GyrC_C
Cuts at: 1185 1599 1847 1882 1185
Size: 414 248 35 3472
Fragments arranged by size:
3472 414 248 35
BanII G_rGCy'C
Cuts at: 999 1183 2213 999
Size: 184 1030 2955
Fragments arranged by size:
2955 1030 184
BbeI G_GCGC'C
Cuts at: 1603 1851 1603
Size: 248 3921
BciVI GTATCCnnnnn_n'
Cuts at: 2067 2828 4169 2067
Size: 761 1341 2067
Fragments arranged by size:
2067 1341 761
BclI T'GATC_A
Cuts at: 317 1689 317
Size: 1372 2797
BglI GCCn_nnn'nGGC
Cuts at: 1115 1115
Size: 4169
BglII A'GATC_T
Cuts at: 555 1684 3721 555
Size: 1129 2037 1003
Fragments arranged by size:
2037 1129 1003
BlpI GC'TnA_GC
Cuts at: 305 310 770 1281 305
Size: 5 460 511 3193
Fragments arranged by size:
3193 511 460 5
Bme1580I G_kGCm'C
Cuts at: 1794 1887 2943 1794
Size: 93 1056 3020
Fragments arranged by size:
3020 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1381 1462 1797 3941 1381
Size: 81 335 2144 1609
Fragments arranged by size:
2144 1609 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 325 2536 325
Size: 2211 1958
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 796 916 929 796
Size: 120 13 4036
Fragments arranged by size:
4036 120 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 1345 2224 2716 3014 109
Size: 1236 879 492 298 1264
Fragments arranged by size:
1264 1236 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 940 3873 940
Size: 2933 1236
BsaAI yAC'GTr
Cuts at: 1522 1522
Size: 4169
BsaBI GATnn'nnATC
Cuts at: 6 1683 3720 6
Size: 1677 2037 455
Fragments arranged by size:
2037 1677 455
BsaHI Gr'CG_yC
Cuts at: 600 1600 1848 2550 4046 600
Size: 1000 248 702 1496 723
Fragments arranged by size:
1496 1000 723 702 248
BsaWI w'CCGG_w
Cuts at: 642 1358 1879 2557 2831 2978 3389 642
Size: 716 521 678 274 147 411 1422
Fragments arranged by size:
1422 716 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 936 966 936
Size: 30 4139
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 188 188
Size: 4169
BseYI C'CCAG_C
Cuts at: 441 1594 2352 2929 3425 441
Size: 1153 758 577 496 1185
Fragments arranged by size:
1185 1153 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 740 740
Size: 4169
BsiEI CG_ry'CG
Cuts at: 104 1711 1757 2965 104
Size: 1607 46 1208 1308
Fragments arranged by size:
1607 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 999 1183 1961 2151 2943 999
Size: 184 778 190 792 2225
Fragments arranged by size:
2225 792 778 190 184
Bsp1286I G_dGCh'C
Cuts at: 999 1183 1794 1887 1961 2151 2213 2943 999
Size: 184 611 93 74 190 62 730 2225
Fragments arranged by size:
2225 730 611 190 184 93 74 62
BspEI T'CCGG_A
Cuts at: 642 3389 642
Size: 2747 1422
BspHI T'CATG_A
Cuts at: 546 3345 546
Size: 2799 1370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1219 1433 1735 2116 1219
Size: 214 302 381 3272
Fragments arranged by size:
3272 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2461 2515 3999 4164 50
Size: 2411 54 1484 165 55
Fragments arranged by size:
2411 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2081 2081
Size: 4169
BsrFI r'CCGG_y
Cuts at: 118 2167 2348 2557 118
Size: 2049 181 209 1730
Fragments arranged by size:
2049 1730 209 181
BssSI C'ACGA_G
Cuts at: 2440 2798 2440
Size: 358 3811
BstAPI GCAn_nnn'nTGC
Cuts at: 1546 1546
Size: 4169
BstBI TT'CG_AA
Cuts at: 2530 2530
Size: 4169
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4169
BstYI r'GATC_y
Cuts at: 1 555 800 1194 1661 1684 2019 2265 3277
Size: 554 245 394 467 23 335 246 1012
Cuts at: 3277 3363 3375 3721 1
Size: 86 12 346 449
Fragments arranged by size:
1012 554 467 449 394 346 335 246
245 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 368 368
Size: 4169
BtgI C'CryG_G
Cuts at: 339 339
Size: 4169
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2119 2300 2463 2119
Size: 181 163 3825
Fragments arranged by size:
3825 181 163
BtsI GCAGTG_nn'
Cuts at: 1412 1412
Size: 4169
DraI TTT'AAA
Cuts at: 1041 1168 1041
Size: 127 4042
DraIII CAC_nnn'GTG
Cuts at: 451 451
Size: 4169
DrdI GACnn_nn'nnGTC
Cuts at: 1875 2733 1875
Size: 858 3311
EaeI y'GGCC_r
Cuts at: 116 342 1107 1754 1928 2319 2346 3691 116
Size: 226 765 647 174 391 27 1345 594
Fragments arranged by size:
1345 765 647 594 391 226 174 27
EagI C'GGCC_G
Cuts at: 1754 1754
Size: 4169
EarI CTCTTCn'nnn_
Cuts at: 405 539 644 2192 2402 405
Size: 134 105 1548 210 2172
Fragments arranged by size:
2172 1548 210 134 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2377 2687 2833 3979 2377
Size: 310 146 1146 2567
Fragments arranged by size:
2567 1146 310 146
EcoICRI GAG'CTC
Cuts at: 997 1181 997
Size: 184 3985
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 520 796 916 929 947 1994 2426 3173
Size: 320 276 120 13 18 1047 432 747
Cuts at: 3173 3441 200
Size: 268 928
Fragments arranged by size:
1047 928 747 432 320 276 268 120
18 13
EcoO109I rG'GnC_Cy
Cuts at: 1308 1308
Size: 4169
EcoRI G'AATT_C
Cuts at: 1173 1173
Size: 4169
EcoRV GAT'ATC
Cuts at: 1053 1053
Size: 4169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 537 863 2188 537
Size: 326 1325 2518
Fragments arranged by size:
2518 1325 326
FseI GG_CCGG'CC
Cuts at: 122 122
Size: 4169
FspI TGC'GCA
Cuts at: 1950 1950
Size: 4169
HaeII r_GCGC'y
Cuts at: 382 1603 1851 2873 3852 382
Size: 1221 248 1022 979 699
Fragments arranged by size:
1221 1022 979 699 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2011 2041 2043 2073 2011
Size: 30 2 30 4107
Fragments arranged by size:
4107 30 30 2
HincII GTy'rAC
Cuts at: 598 1208 598
Size: 610 3559
KasI G'GCGC_C
Cuts at: 1599 1847 1599
Size: 248 3921
KpnI G_GTAC'C
Cuts at: 1189 1189
Size: 4169
MfeI C'AATT_G
Cuts at: 269 269
Size: 4169
MluI A'CGCG_T
Cuts at: 3602 3602
Size: 4169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 150 461 569 928 2840 3024 150
Size: 311 108 359 1912 184 1295
Fragments arranged by size:
1912 1295 359 311 184 108
MscI TGG'CCA
Cuts at: 344 1109 1930 344
Size: 765 821 2583
Fragments arranged by size:
2583 821 765
MslI CAynn'nnrTG
Cuts at: 112 508 2158 2285 112
Size: 396 1650 127 1996
Fragments arranged by size:
1996 1650 396 127
MspA1I CmG'CkG
Cuts at: 774 1280 1594 1954 2967 3212 774
Size: 506 314 360 1013 245 1731
Fragments arranged by size:
1731 1013 506 360 314 245
NaeI GCC'GGC
Cuts at: 120 2350 120
Size: 2230 1939
NarI GG'CG_CC
Cuts at: 1600 1848 1600
Size: 248 3921
NgoMIV G'CCGG_C
Cuts at: 118 2348 118
Size: 2230 1939
NspI r_CATG'y
Cuts at: 711 1222 1722 2629 711
Size: 511 500 907 2251
Fragments arranged by size:
2251 907 511 500
PciI A'CATG_T
Cuts at: 2625 2625
Size: 4169
PflMI CCAn_nnn'nTGG
Cuts at: 448 448
Size: 4169
PmeI GTTT'AAAC
Cuts at: 1168 1168
Size: 4169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 4137
PsiI TTA'TAA
Cuts at: 617 617
Size: 4169
PstI C_TGCA'G
Cuts at: 809 1216 1901 809
Size: 407 685 3077
Fragments arranged by size:
3077 685 407
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4169
PvuII CAG'CTG
Cuts at: 774 1594 1954 774
Size: 820 360 2989
Fragments arranged by size:
2989 820 360
RsrII CG'GwC_CG
Cuts at: 2364 2364
Size: 4169
SacI G_AGCT'C
Cuts at: 999 1183 999
Size: 184 3985
SalI G'TCGA_C
Cuts at: 596 1206 596
Size: 610 3559
SapI GCTCTTCn'nnn_
Cuts at: 644 2192 2402 644
Size: 1548 210 2411
Fragments arranged by size:
2411 1548 210
SbfI CC_TGCA'GG
Cuts at: 1216 1216
Size: 4169
ScaI AGT'ACT
Cuts at: 918 3686 918
Size: 2768 1401
SfcI C'TryA_G
Cuts at: 33 805 1212 1897 2890 3081 33
Size: 772 407 685 993 191 1121
Fragments arranged by size:
1121 993 772 685 407 191
SfoI GGC'GCC
Cuts at: 1601 1849 1601
Size: 248 3921
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
SmaI CCC'GGG
Cuts at: 1191 1191
Size: 4169
SmlI C'TyrA_G
Cuts at: 124 1324 2239 2731 2993 124
Size: 1200 915 492 262 1300
Fragments arranged by size:
1300 1200 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4169
SphI G_CATG'C
Cuts at: 1222 1722 1222
Size: 500 3669
StyI C'CwwG_G
Cuts at: 1303 1657 1303
Size: 354 3815
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1752 1885 2344 2553 1752
Size: 133 459 209 3368
Fragments arranged by size:
3368 459 209 133
TatI w'GTAC_w
Cuts at: 916 3684 916
Size: 2768 1401
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 936 968 3686 3718 936
Size: 32 2718 32 1387
Fragments arranged by size:
2718 1387 32 32
Tth111I GACn'n_nGTC
Cuts at: 1966 1966
Size: 4169
XbaI T'CTAG_A
Cuts at: 666 1200 666
Size: 534 3635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1134 1664 1134
Size: 530 3639
XmaI C'CCGG_G
Cuts at: 1189 1189
Size: 4169
XmnI GAAnn'nnTTC
Cuts at: 133 278 653 133
Size: 145 375 3649
Fragments arranged by size:
3649 375 145
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI EcoNI FspAI HindIII HpaI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI