Restriction map for
pF1KB6460
(Circular) (Six-base) MAPSORT of: pF1KB6460.seq Check: 5469 from: 1 to: 4166
pF1KB6460 4166 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 1204 1204
Size: 4166
Acc65I G'GTAC_C
Cuts at: 1182 1182
Size: 4166
AclI AA'CG_TT
Cuts at: 4006 4006
Size: 4166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 876 1991 2423 3170 3438 631
Size: 245 1115 432 747 268 1359
Fragments arranged by size:
1359 1115 747 432 268 245
AfeI AGC'GCT
Cuts at: 949 949
Size: 4166
AflIII A'CryG_T
Cuts at: 1518 2622 3599 1518
Size: 1104 977 2085
Fragments arranged by size:
2085 1104 977
AgeI A'CCGG_T
Cuts at: 2554 2554
Size: 4166
AlwNI CAG_nnn'CTG
Cuts at: 1471 3038 1471
Size: 1567 2599
ApaI G_GGCC'C
Cuts at: 780 780
Size: 4166
ApaLI G'TGCA_C
Cuts at: 2936 2936
Size: 4166
ApoI r'AATT_y
Cuts at: 160 1170 3519 3775 160
Size: 1010 2349 256 551
Fragments arranged by size:
2349 1010 551 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4166
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
AvaI C'yCGr_G
Cuts at: 427 1186 427
Size: 759 3407
BamHI G'GATC_C
Cuts at: 1191 1191
Size: 4166
BanI G'GyrC_C
Cuts at: 709 1182 1596 1844 1879 709
Size: 473 414 248 35 2996
Fragments arranged by size:
2996 473 414 248 35
BanII G_rGCy'C
Cuts at: 268 533 780 1180 2210 268
Size: 265 247 400 1030 2224
Fragments arranged by size:
2224 1030 400 265 247
BbeI G_GCGC'C
Cuts at: 1600 1848 1600
Size: 248 3918
BciVI GTATCCnnnnn_n'
Cuts at: 1071 2064 2825 4166 1071
Size: 993 761 1341 1071
Fragments arranged by size:
1341 1071 993 761
BclI T'GATC_A
Cuts at: 1686 1686
Size: 4166
BglII A'GATC_T
Cuts at: 845 936 1681 3718 845
Size: 91 745 2037 1293
Fragments arranged by size:
2037 1293 745 91
BlpI GC'TnA_GC
Cuts at: 1278 1278
Size: 4166
Bme1580I G_kGCm'C
Cuts at: 482 780 1791 1884 2940 482
Size: 298 1011 93 1056 1708
Fragments arranged by size:
1708 1056 1011 298 93
BmrI ACTGGGnnnn_n'
Cuts at: 1378 1459 1794 3938 1378
Size: 81 335 2144 1606
Fragments arranged by size:
2144 1606 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 513 2533 513
Size: 2020 2146
Bpu10I CC'TnA_GC
Cuts at: 742 742
Size: 4166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1342 2221 2713 3011 946
Size: 396 879 492 298 2101
Fragments arranged by size:
2101 879 492 396 298
BsaI GGTCTCn'nnnn_
Cuts at: 295 1125 3870 295
Size: 830 2745 591
Fragments arranged by size:
2745 830 591
BsaAI yAC'GTr
Cuts at: 477 1519 477
Size: 1042 3124
BsaBI GATnn'nnATC
Cuts at: 6 1021 1680 3717 6
Size: 1015 659 2037 455
Fragments arranged by size:
2037 1015 659 455
BsaHI Gr'CG_yC
Cuts at: 1597 1845 2547 4043 1597
Size: 248 702 1496 1720
Fragments arranged by size:
1720 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1355 1876 2554 2828 2975 3386 1355
Size: 521 678 274 147 411 2135
Fragments arranged by size:
2135 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1591 2349 2926 3422 1591
Size: 758 577 496 2335
Fragments arranged by size:
2335 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 565
Size: 4166
BsiEI CG_ry'CG
Cuts at: 104 1708 1754 2962 104
Size: 1604 46 1208 1308
Fragments arranged by size:
1604 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1180 1958 2148 2940 1180
Size: 778 190 792 2406
Fragments arranged by size:
2406 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 867 867
Size: 4166
Bsp1286I G_dGCh'C
Cuts at: 268 482 533 780 1180 1791 1884 1958 2148
Size: 214 51 247 400 611 93 74 190
Cuts at: 2148 2210 2940 268
Size: 62 730 1494
Fragments arranged by size:
1494 730 611 400 247 214 190 93
74 62 51
BspEI T'CCGG_A
Cuts at: 3386 3386
Size: 4166
BspHI T'CATG_A
Cuts at: 3342 3342
Size: 4166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 920 921 1216 1430 1732 2113 920
Size: 1 295 214 302 381 2973
Fragments arranged by size:
2973 381 302 295 214 1
BsrBI CCG'CTC
Cuts at: 50 211 2458 2512 3996 4161 50
Size: 161 2247 54 1484 165 55
Fragments arranged by size:
2247 1484 165 161 55 54
BsrDI GCAATG_nn'
Cuts at: 2078 2078
Size: 4166
BsrFI r'CCGG_y
Cuts at: 2164 2345 2554 2164
Size: 181 209 3776
Fragments arranged by size:
3776 209 181
BssSI C'ACGA_G
Cuts at: 2437 2795 2437
Size: 358 3808
BstAPI GCAn_nnn'nTGC
Cuts at: 1543 1543
Size: 4166
BstBI TT'CG_AA
Cuts at: 2527 2527
Size: 4166
BstYI r'GATC_y
Cuts at: 1 326 591 845 936 1191 1658 1681 2016
Size: 325 265 254 91 255 467 23 335
Cuts at: 2016 2262 3274 3360 3372 3718 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 325 265 255
254 246 91 86 23 12
BtgI C'CryG_G
Cuts at: 107 764 107
Size: 657 3509
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2116 2297 2460 2116
Size: 181 163 3822
Fragments arranged by size:
3822 181 163
BtsI GCAGTG_nn'
Cuts at: 1409 1409
Size: 4166
DraI TTT'AAA
Cuts at: 1065 1165 1065
Size: 100 4066
DrdI GACnn_nn'nnGTC
Cuts at: 1872 2730 1872
Size: 858 3308
EaeI y'GGCC_r
Cuts at: 803 1751 1925 2316 2343 3688 803
Size: 948 174 391 27 1345 1281
Fragments arranged by size:
1345 1281 948 391 174 27
EagI C'GGCC_G
Cuts at: 1751 1751
Size: 4166
EarI CTCTTCn'nnn_
Cuts at: 2189 2399 2189
Size: 210 3956
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 525 2374 2684 2830 3976 525
Size: 1849 310 146 1146 715
Fragments arranged by size:
1849 1146 715 310 146
EcoICRI GAG'CTC
Cuts at: 1178 1178
Size: 4166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 876 1991 2423 3170 3438 631
Size: 245 1115 432 747 268 1359
Fragments arranged by size:
1359 1115 747 432 268 245
EcoNI CCTnn'n_nnAGG
Cuts at: 928 928
Size: 4166
EcoO109I rG'GnC_Cy
Cuts at: 606 777 909 1069 1305 606
Size: 171 132 160 236 3467
Fragments arranged by size:
3467 236 171 160 132
EcoRI G'AATT_C
Cuts at: 160 1170 160
Size: 1010 3156
EcoRV GAT'ATC
Cuts at: 981 981
Size: 4166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 205 894 2185 205
Size: 689 1291 2186
Fragments arranged by size:
2186 1291 689
FspI TGC'GCA
Cuts at: 1947 1947
Size: 4166
HaeII r_GCGC'y
Cuts at: 951 1600 1848 2870 3849 951
Size: 649 248 1022 979 1268
Fragments arranged by size:
1268 1022 979 649 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2008 2038 2040 2070 2008
Size: 30 2 30 4104
Fragments arranged by size:
4104 30 30 2
HincII GTy'rAC
Cuts at: 405 975 1205 405
Size: 570 230 3366
Fragments arranged by size:
3366 570 230
KasI G'GCGC_C
Cuts at: 1596 1844 1596
Size: 248 3918
KpnI G_GTAC'C
Cuts at: 1186 1186
Size: 4166
MluI A'CGCG_T
Cuts at: 3599 3599
Size: 4166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2837 3021 2837
Size: 184 3982
MscI TGG'CCA
Cuts at: 1927 1927
Size: 4166
MslI CAynn'nnrTG
Cuts at: 466 652 2155 2282 466
Size: 186 1503 127 2350
Fragments arranged by size:
2350 1503 186 127
MspA1I CmG'CkG
Cuts at: 808 1277 1591 1951 2964 3209 808
Size: 469 314 360 1013 245 1765
Fragments arranged by size:
1765 1013 469 360 314 245
NaeI GCC'GGC
Cuts at: 2347 2347
Size: 4166
NarI GG'CG_CC
Cuts at: 1597 1845 1597
Size: 248 3918
NcoI C'CATG_G
Cuts at: 107 107
Size: 4166
NgoMIV G'CCGG_C
Cuts at: 2345 2345
Size: 4166
NspI r_CATG'y
Cuts at: 1219 1719 2626 1219
Size: 500 907 2759
Fragments arranged by size:
2759 907 500
PasI CC'CwG_GG
Cuts at: 780 1072 780
Size: 292 3874
PciI A'CATG_T
Cuts at: 2622 2622
Size: 4166
PmeI GTTT'AAAC
Cuts at: 1165 1165
Size: 4166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 4134
PpuMI rG'GwC_Cy
Cuts at: 606 909 606
Size: 303 3863
PspOMI G'GGCC_C
Cuts at: 776 776
Size: 4166
PspXI vC'TCGA_Gb
Cuts at: 427 427
Size: 4166
PstI C_TGCA'G
Cuts at: 932 1213 1898 932
Size: 281 685 3200
Fragments arranged by size:
3200 685 281
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4166
PvuII CAG'CTG
Cuts at: 1591 1951 1591
Size: 360 3806
RsrII CG'GwC_CG
Cuts at: 2361 2361
Size: 4166
SacI G_AGCT'C
Cuts at: 1180 1180
Size: 4166
SalI G'TCGA_C
Cuts at: 1203 1203
Size: 4166
SapI GCTCTTCn'nnn_
Cuts at: 2189 2399 2189
Size: 210 3956
SbfI CC_TGCA'GG
Cuts at: 932 1213 932
Size: 281 3885
ScaI AGT'ACT
Cuts at: 3683 3683
Size: 4166
SfcI C'TryA_G
Cuts at: 33 600 928 1209 1894 2887 3078 33
Size: 567 328 281 685 993 191 1121
Fragments arranged by size:
1121 993 685 567 328 281 191
SfoI GGC'GCC
Cuts at: 1598 1846 1598
Size: 248 3918
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
SmaI CCC'GGG
Cuts at: 1188 1188
Size: 4166
SmlI C'TyrA_G
Cuts at: 427 961 1321 2236 2728 2990 427
Size: 534 360 915 492 262 1603
Fragments arranged by size:
1603 915 534 492 360 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4166
SphI G_CATG'C
Cuts at: 1219 1719 1219
Size: 500 3666
StyI C'CwwG_G
Cuts at: 107 1300 1654 107
Size: 1193 354 2619
Fragments arranged by size:
2619 1193 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1749 1882 2341 2550 1749
Size: 133 459 209 3365
Fragments arranged by size:
3365 459 209 133
TatI w'GTAC_w
Cuts at: 3681 3681
Size: 4166
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3683 3715 3683
Size: 32 4134
Tth111I GACn'n_nGTC
Cuts at: 1963 1963
Size: 4166
XbaI T'CTAG_A
Cuts at: 1197 1197
Size: 4166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1661 1661
Size: 4166
XhoI C'TCGA_G
Cuts at: 427 427
Size: 4166
XmaI C'CCGG_G
Cuts at: 1186 1186
Size: 4166
XmnI GAAnn'nnTTC
Cuts at: 160 185 160
Size: 25 4141
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI BsaXI BseRI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI ZraI