Restriction map for
pF1KB6458
(Circular) (Six-base) MAPSORT of: pF1KB6458.seq Check: 7822 from: 1 to: 4166
pF1KB6458 4166 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 332 1204 332
Size: 872 3294
Acc65I G'GTAC_C
Cuts at: 1182 1182
Size: 4166
AclI AA'CG_TT
Cuts at: 4006 4006
Size: 4166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 867 1100 1991 2423 3170 3438 367
Size: 500 233 891 432 747 268 1095
Fragments arranged by size:
1095 891 747 500 432 268 233
AflIII A'CryG_T
Cuts at: 1518 2622 3599 1518
Size: 1104 977 2085
Fragments arranged by size:
2085 1104 977
AgeI A'CCGG_T
Cuts at: 2554 2554
Size: 4166
AhdI GACnn_n'nnGTC
Cuts at: 300 300
Size: 4166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 883 893 883
Size: 10 4156
AlwNI CAG_nnn'CTG
Cuts at: 1471 3038 1471
Size: 1567 2599
ApaI G_GGCC'C
Cuts at: 829 829
Size: 4166
ApaLI G'TGCA_C
Cuts at: 2936 2936
Size: 4166
ApoI r'AATT_y
Cuts at: 431 1170 3519 3775 431
Size: 739 2349 256 822
Fragments arranged by size:
2349 822 739 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4166
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
AvaI C'yCGr_G
Cuts at: 311 1186 311
Size: 875 3291
BamHI G'GATC_C
Cuts at: 753 1191 753
Size: 438 3728
BanI G'GyrC_C
Cuts at: 1182 1596 1844 1879 1182
Size: 414 248 35 3469
Fragments arranged by size:
3469 414 248 35
BanII G_rGCy'C
Cuts at: 829 1180 2210 829
Size: 351 1030 2785
Fragments arranged by size:
2785 1030 351
BbeI G_GCGC'C
Cuts at: 1600 1848 1600
Size: 248 3918
BbvCI CC'TCA_GC
Cuts at: 425 425
Size: 4166
BciVI GTATCCnnnnn_n'
Cuts at: 2064 2825 4166 2064
Size: 761 1341 2064
Fragments arranged by size:
2064 1341 761
BclI T'GATC_A
Cuts at: 1686 1686
Size: 4166
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4166
BglII A'GATC_T
Cuts at: 366 1681 3718 366
Size: 1315 2037 814
Fragments arranged by size:
2037 1315 814
BlpI GC'TnA_GC
Cuts at: 1278 1278
Size: 4166
Bme1580I G_kGCm'C
Cuts at: 829 1791 1884 2940 829
Size: 962 93 1056 2055
Fragments arranged by size:
2055 1056 962 93
BmrI ACTGGGnnnn_n'
Cuts at: 577 796 1378 1459 1794 3938 577
Size: 219 582 81 335 2144 805
Fragments arranged by size:
2144 805 582 335 219 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2533 2533
Size: 4166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 865
Size: 4166
Bpu10I CC'TnA_GC
Cuts at: 425 425
Size: 4166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 1342 2221 2713 3011 541
Size: 801 879 492 298 1696
Fragments arranged by size:
1696 879 801 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 456 3870 456
Size: 3414 752
BsaAI yAC'GTr
Cuts at: 1519 1519
Size: 4166
BsaBI GATnn'nnATC
Cuts at: 6 752 1680 3717 6
Size: 746 928 2037 455
Fragments arranged by size:
2037 928 746 455
BsaHI Gr'CG_yC
Cuts at: 1597 1845 2547 4043 1597
Size: 248 702 1496 1720
Fragments arranged by size:
1720 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1355 1876 2554 2828 2975 3386 1355
Size: 521 678 274 147 411 2135
Fragments arranged by size:
2135 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 595 670 595
Size: 75 4091
BseYI C'CCAG_C
Cuts at: 935 1591 2349 2926 3422 935
Size: 656 758 577 496 1679
Fragments arranged by size:
1679 758 656 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 308 1037 308
Size: 729 3437
BsiEI CG_ry'CG
Cuts at: 104 1708 1754 2962 104
Size: 1604 46 1208 1308
Fragments arranged by size:
1604 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1180 1958 2148 2940 1180
Size: 778 190 792 2406
Fragments arranged by size:
2406 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 829 1180 1791 1884 1958 2148 2210 2940 829
Size: 351 611 93 74 190 62 730 2055
Fragments arranged by size:
2055 730 611 351 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3386 3386
Size: 4166
BspHI T'CATG_A
Cuts at: 3342 3342
Size: 4166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 157 1216 1430 1732 2113 157
Size: 1059 214 302 381 2210
Fragments arranged by size:
2210 1059 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2458 2512 3996 4161 50
Size: 2408 54 1484 165 55
Fragments arranged by size:
2408 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 502 2078 502
Size: 1576 2590
BsrFI r'CCGG_y
Cuts at: 2164 2345 2554 2164
Size: 181 209 3776
Fragments arranged by size:
3776 209 181
BssSI C'ACGA_G
Cuts at: 2437 2795 2437
Size: 358 3808
BstAPI GCAn_nnn'nTGC
Cuts at: 1543 1543
Size: 4166
BstBI TT'CG_AA
Cuts at: 2527 2527
Size: 4166
BstXI CCAn_nnnn'nTGG
Cuts at: 943 943
Size: 4166
BstYI r'GATC_y
Cuts at: 1 366 753 1191 1658 1681 2016 2262 3274
Size: 365 387 438 467 23 335 246 1012
Cuts at: 3274 3360 3372 3718 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 438 387 365 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 446 951 107
Size: 339 505 3322
Fragments arranged by size:
3322 505 339
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2116 2297 2460 2116
Size: 181 163 3822
Fragments arranged by size:
3822 181 163
BtsI GCAGTG_nn'
Cuts at: 617 800 1043 1409 617
Size: 183 243 366 3374
Fragments arranged by size:
3374 366 243 183
ClaI AT'CG_AT
Cuts at: 440 440
Size: 4166
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 286 321 286
Size: 35 4131
DraI TTT'AAA
Cuts at: 1165 1165
Size: 4166
DraIII CAC_nnn'GTG
Cuts at: 922 922
Size: 4166
DrdI GACnn_nn'nnGTC
Cuts at: 1872 2730 1872
Size: 858 3308
EaeI y'GGCC_r
Cuts at: 1126 1751 1925 2316 2343 3688 1126
Size: 625 174 391 27 1345 1604
Fragments arranged by size:
1604 1345 625 391 174 27
EagI C'GGCC_G
Cuts at: 1751 1751
Size: 4166
EarI CTCTTCn'nnn_
Cuts at: 343 685 2189 2399 343
Size: 342 1504 210 2110
Fragments arranged by size:
2110 1504 342 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2374 2684 2830 3976 2374
Size: 310 146 1146 2564
Fragments arranged by size:
2564 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1178 1178
Size: 4166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 865 867 1100 1991 2423 3170 3438 367
Size: 498 2 233 891 432 747 268 1095
Fragments arranged by size:
1095 891 747 498 432 268 233 2
EcoO109I rG'GnC_Cy
Cuts at: 825 826 903 1305 825
Size: 1 77 402 3686
Fragments arranged by size:
3686 402 77 1
EcoRI G'AATT_C
Cuts at: 1170 1170
Size: 4166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 270 2185 270
Size: 1915 2251
FspI TGC'GCA
Cuts at: 1947 1947
Size: 4166
HaeII r_GCGC'y
Cuts at: 1600 1848 2870 3849 1600
Size: 248 1022 979 1917
Fragments arranged by size:
1917 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 286 318 2008 2038 2040 2070 286
Size: 32 1690 30 2 30 2382
Fragments arranged by size:
2382 1690 32 30 30 2
HincII GTy'rAC
Cuts at: 1205 1205
Size: 4166
KasI G'GCGC_C
Cuts at: 1596 1844 1596
Size: 248 3918
KpnI G_GTAC'C
Cuts at: 1186 1186
Size: 4166
MluI A'CGCG_T
Cuts at: 3599 3599
Size: 4166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 389 2837 3021 389
Size: 2448 184 1534
Fragments arranged by size:
2448 1534 184
MscI TGG'CCA
Cuts at: 1128 1927 1128
Size: 799 3367
MslI CAynn'nnrTG
Cuts at: 194 2155 2282 194
Size: 1961 127 2078
Fragments arranged by size:
2078 1961 127
MspA1I CmG'CkG
Cuts at: 144 887 1277 1591 1951 2964 3209 144
Size: 743 390 314 360 1013 245 1101
Fragments arranged by size:
1101 1013 743 390 360 314 245
NaeI GCC'GGC
Cuts at: 2347 2347
Size: 4166
NarI GG'CG_CC
Cuts at: 1597 1845 1597
Size: 248 3918
NcoI C'CATG_G
Cuts at: 107 951 107
Size: 844 3322
NgoMIV G'CCGG_C
Cuts at: 2345 2345
Size: 4166
NspI r_CATG'y
Cuts at: 870 1219 1719 2626 870
Size: 349 500 907 2410
Fragments arranged by size:
2410 907 500 349
PasI CC'CwG_GG
Cuts at: 837 837
Size: 4166
PciI A'CATG_T
Cuts at: 2622 2622
Size: 4166
PmeI GTTT'AAAC
Cuts at: 1165 1165
Size: 4166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 115 147 3305 3337 115
Size: 32 3158 32 944
Fragments arranged by size:
3158 944 32 32
PspOMI G'GGCC_C
Cuts at: 825 825
Size: 4166
PstI C_TGCA'G
Cuts at: 795 1068 1213 1898 795
Size: 273 145 685 3063
Fragments arranged by size:
3063 685 273 145
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4166
PvuII CAG'CTG
Cuts at: 1591 1951 1591
Size: 360 3806
RsrII CG'GwC_CG
Cuts at: 2361 2361
Size: 4166
SacI G_AGCT'C
Cuts at: 1180 1180
Size: 4166
SalI G'TCGA_C
Cuts at: 1203 1203
Size: 4166
SapI GCTCTTCn'nnn_
Cuts at: 2189 2399 2189
Size: 210 3956
SbfI CC_TGCA'GG
Cuts at: 1213 1213
Size: 4166
ScaI AGT'ACT
Cuts at: 3683 3683
Size: 4166
SfcI C'TryA_G
Cuts at: 33 791 1064 1209 1894 2887 3078 33
Size: 758 273 145 685 993 191 1121
Fragments arranged by size:
1121 993 758 685 273 191 145
SfoI GGC'GCC
Cuts at: 1598 1846 1598
Size: 248 3918
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
SmaI CCC'GGG
Cuts at: 1188 1188
Size: 4166
SmlI C'TyrA_G
Cuts at: 556 1321 2236 2728 2990 556
Size: 765 915 492 262 1732
Fragments arranged by size:
1732 915 765 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4166
SphI G_CATG'C
Cuts at: 870 1219 1719 870
Size: 349 500 3317
Fragments arranged by size:
3317 500 349
StyI C'CwwG_G
Cuts at: 107 951 1300 1654 107
Size: 844 349 354 2619
Fragments arranged by size:
2619 844 354 349
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 526 1749 1882 2341 2550 526
Size: 1223 133 459 209 2142
Fragments arranged by size:
2142 1223 459 209 133
TatI w'GTAC_w
Cuts at: 376 3681 376
Size: 3305 861
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 932 964 3683 3715 932
Size: 32 2719 32 1383
Fragments arranged by size:
2719 1383 32 32
Tth111I GACn'n_nGTC
Cuts at: 1963 1963
Size: 4166
XbaI T'CTAG_A
Cuts at: 1197 1197
Size: 4166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1661 1661
Size: 4166
XmaI C'CCGG_G
Cuts at: 1186 1186
Size: 4166
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI