Restriction map for
pF1KB6457
(Circular) (Six-base) MAPSORT of: pF1KB6457.seq Check: 6284 from: 1 to: 4067
pF1KB6457 4067 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 904 1105 904
Size: 201 3866
Acc65I G'GTAC_C
Cuts at: 1083 1083
Size: 4067
AclI AA'CG_TT
Cuts at: 147 3907 147
Size: 3760 307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 1892 2324 3071 3339 148
Size: 1744 432 747 268 876
Fragments arranged by size:
1744 876 747 432 268
AflIII A'CryG_T
Cuts at: 1419 2523 3500 1419
Size: 1104 977 1986
Fragments arranged by size:
1986 1104 977
AgeI A'CCGG_T
Cuts at: 2455 2455
Size: 4067
AlwNI CAG_nnn'CTG
Cuts at: 1372 2939 1372
Size: 1567 2500
ApaI G_GGCC'C
Cuts at: 251 879 251
Size: 628 3439
ApaLI G'TGCA_C
Cuts at: 2837 2837
Size: 4067
ApoI r'AATT_y
Cuts at: 798 1071 3420 3676 798
Size: 273 2349 256 1189
Fragments arranged by size:
2349 1189 273 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4067
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
AvaI C'yCGr_G
Cuts at: 1087 1087
Size: 4067
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 292 325 292
Size: 33 4034
BamHI G'GATC_C
Cuts at: 1092 1092
Size: 4067
BanI G'GyrC_C
Cuts at: 1083 1497 1745 1780 1083
Size: 414 248 35 3370
Fragments arranged by size:
3370 414 248 35
BanII G_rGCy'C
Cuts at: 251 862 879 1081 2111 251
Size: 611 17 202 1030 2207
Fragments arranged by size:
2207 1030 611 202 17
BbeI G_GCGC'C
Cuts at: 1501 1749 1501
Size: 248 3819
BbvCI CC'TCA_GC
Cuts at: 804 804
Size: 4067
BciVI GTATCCnnnnn_n'
Cuts at: 320 1965 2726 4067 320
Size: 1645 761 1341 320
Fragments arranged by size:
1645 1341 761 320
BclI T'GATC_A
Cuts at: 954 1587 954
Size: 633 3434
BglII A'GATC_T
Cuts at: 1582 3619 1582
Size: 2037 2030
BlpI GC'TnA_GC
Cuts at: 1179 1179
Size: 4067
Bme1580I G_kGCm'C
Cuts at: 251 879 1692 1785 2841 251
Size: 628 813 93 1056 1477
Fragments arranged by size:
1477 1056 813 628 93
BmrI ACTGGGnnnn_n'
Cuts at: 1279 1360 1695 3839 1279
Size: 81 335 2144 1507
Fragments arranged by size:
2144 1507 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4067
Bpu10I CC'TnA_GC
Cuts at: 804 804
Size: 4067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1243 2122 2614 2912 1243
Size: 879 492 298 2398
Fragments arranged by size:
2398 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 106 3771 106
Size: 3665 402
BsaAI yAC'GTr
Cuts at: 1420 1420
Size: 4067
BsaBI GATnn'nnATC
Cuts at: 6 982 1581 3618 6
Size: 976 599 2037 455
Fragments arranged by size:
2037 976 599 455
BsaHI Gr'CG_yC
Cuts at: 1498 1746 2448 3944 1498
Size: 248 702 1496 1621
Fragments arranged by size:
1621 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1256 1777 2455 2729 2876 3287 1256
Size: 521 678 274 147 411 2036
Fragments arranged by size:
2036 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 287 287
Size: 4067
BseYI C'CCAG_C
Cuts at: 878 1492 2250 2827 3323 878
Size: 614 758 577 496 1622
Fragments arranged by size:
1622 758 614 577 496
BsiEI CG_ry'CG
Cuts at: 104 1609 1655 2863 104
Size: 1505 46 1208 1308
Fragments arranged by size:
1505 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 525 1081 1859 2049 2841 525
Size: 556 778 190 792 1751
Fragments arranged by size:
1751 792 778 556 190
BsiWI C'GTAC_G
Cuts at: 785 785
Size: 4067
Bsp1286I G_dGCh'C
Cuts at: 251 525 862 879 1081 1692 1785 1859 2049
Size: 274 337 17 202 611 93 74 190
Cuts at: 2049 2111 2841 251
Size: 62 730 1477
Fragments arranged by size:
1477 730 611 337 274 202 190 93
74 62 17
BspEI T'CCGG_A
Cuts at: 3287 3287
Size: 4067
BspHI T'CATG_A
Cuts at: 3243 3243
Size: 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1117 1331 1633 2014 1117
Size: 214 302 381 3170
Fragments arranged by size:
3170 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2359 2413 3897 4062 50
Size: 2309 54 1484 165 55
Fragments arranged by size:
2309 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1979 1979
Size: 4067
BsrFI r'CCGG_y
Cuts at: 2065 2246 2455 2065
Size: 181 209 3677
Fragments arranged by size:
3677 209 181
BssSI C'ACGA_G
Cuts at: 2338 2696 2338
Size: 358 3709
BstAPI GCAn_nnn'nTGC
Cuts at: 1444 1444
Size: 4067
BstBI TT'CG_AA
Cuts at: 2428 2428
Size: 4067
BstXI CCAn_nnnn'nTGG
Cuts at: 642 642
Size: 4067
BstYI r'GATC_y
Cuts at: 1 177 1092 1559 1582 1917 2163 3175 3261
Size: 176 915 467 23 335 246 1012 86
Cuts at: 3261 3273 3619 1
Size: 12 346 449
Fragments arranged by size:
1012 915 467 449 346 335 246 176
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 509 509
Size: 4067
BtgI C'CryG_G
Cuts at: 107 107
Size: 4067
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2017 2198 2361 2017
Size: 181 163 3723
Fragments arranged by size:
3723 181 163
BtsI GCAGTG_nn'
Cuts at: 1310 1310
Size: 4067
DraI TTT'AAA
Cuts at: 596 1066 596
Size: 470 3597
DrdI GACnn_nn'nnGTC
Cuts at: 1773 2631 1773
Size: 858 3209
EaeI y'GGCC_r
Cuts at: 773 1652 1826 2217 2244 3589 773
Size: 879 174 391 27 1345 1251
Fragments arranged by size:
1345 1251 879 391 174 27
EagI C'GGCC_G
Cuts at: 1652 1652
Size: 4067
EarI CTCTTCn'nnn_
Cuts at: 124 513 2090 2300 124
Size: 389 1577 210 1891
Fragments arranged by size:
1891 1577 389 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 303 2275 2585 2731 3877 303
Size: 1972 310 146 1146 493
Fragments arranged by size:
1972 1146 493 310 146
EcoICRI GAG'CTC
Cuts at: 1079 1079
Size: 4067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 1892 2324 3071 3339 148
Size: 1744 432 747 268 876
Fragments arranged by size:
1744 876 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 247 248 1206 247
Size: 1 958 3108
Fragments arranged by size:
3108 958 1
EcoRI G'AATT_C
Cuts at: 798 1071 798
Size: 273 3794
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 4067
FspI TGC'GCA
Cuts at: 200 1848 200
Size: 1648 2419
HaeII r_GCGC'y
Cuts at: 873 1501 1749 2771 3750 873
Size: 628 248 1022 979 1190
Fragments arranged by size:
1190 1022 979 628 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1909 1939 1941 1971 1909
Size: 30 2 30 4005
Fragments arranged by size:
4005 30 30 2
HincII GTy'rAC
Cuts at: 1106 1106
Size: 4067
HindIII A'AGCT_T
Cuts at: 657 657
Size: 4067
KasI G'GCGC_C
Cuts at: 1497 1745 1497
Size: 248 3819
KpnI G_GTAC'C
Cuts at: 1087 1087
Size: 4067
MluI A'CGCG_T
Cuts at: 3500 3500
Size: 4067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 205 1041 2738 2922 205
Size: 836 1697 184 1350
Fragments arranged by size:
1697 1350 836 184
MscI TGG'CCA
Cuts at: 1828 1828
Size: 4067
MslI CAynn'nnrTG
Cuts at: 194 2056 2183 194
Size: 1862 127 2078
Fragments arranged by size:
2078 1862 127
MspA1I CmG'CkG
Cuts at: 970 1178 1492 1852 2865 3110 970
Size: 208 314 360 1013 245 1927
Fragments arranged by size:
1927 1013 360 314 245 208
NaeI GCC'GGC
Cuts at: 2248 2248
Size: 4067
NarI GG'CG_CC
Cuts at: 1498 1746 1498
Size: 248 3819
NcoI C'CATG_G
Cuts at: 107 107
Size: 4067
NgoMIV G'CCGG_C
Cuts at: 2246 2246
Size: 4067
NspI r_CATG'y
Cuts at: 1120 1620 2527 1120
Size: 500 907 2660
Fragments arranged by size:
2660 907 500
PciI A'CATG_T
Cuts at: 2523 2523
Size: 4067
PmeI GTTT'AAAC
Cuts at: 1066 1066
Size: 4067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3206 3238 3206
Size: 32 4035
PspOMI G'GGCC_C
Cuts at: 247 875 247
Size: 628 3439
PstI C_TGCA'G
Cuts at: 1114 1799 1114
Size: 685 3382
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4067
PvuII CAG'CTG
Cuts at: 970 1492 1852 970
Size: 522 360 3185
Fragments arranged by size:
3185 522 360
RsrII CG'GwC_CG
Cuts at: 2262 2262
Size: 4067
SacI G_AGCT'C
Cuts at: 1081 1081
Size: 4067
SalI G'TCGA_C
Cuts at: 1104 1104
Size: 4067
SapI GCTCTTCn'nnn_
Cuts at: 513 2090 2300 513
Size: 1577 210 2280
Fragments arranged by size:
2280 1577 210
SbfI CC_TGCA'GG
Cuts at: 1114 1114
Size: 4067
ScaI AGT'ACT
Cuts at: 296 3584 296
Size: 3288 779
SfcI C'TryA_G
Cuts at: 33 836 1110 1795 2788 2979 33
Size: 803 274 685 993 191 1121
Fragments arranged by size:
1121 993 803 685 274 191
SfoI GGC'GCC
Cuts at: 1499 1747 1499
Size: 248 3819
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
SmaI CCC'GGG
Cuts at: 1089 1089
Size: 4067
SmlI C'TyrA_G
Cuts at: 1222 2137 2629 2891 1222
Size: 915 492 262 2398
Fragments arranged by size:
2398 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4067
SphI G_CATG'C
Cuts at: 1120 1620 1120
Size: 500 3567
StyI C'CwwG_G
Cuts at: 107 1201 1555 107
Size: 1094 354 2619
Fragments arranged by size:
2619 1094 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1650 1783 2242 2451 1650
Size: 133 459 209 3266
Fragments arranged by size:
3266 459 209 133
TatI w'GTAC_w
Cuts at: 294 778 972 3582 294
Size: 484 194 2610 779
Fragments arranged by size:
2610 779 484 194
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3584 3616 3584
Size: 32 4035
Tth111I GACn'n_nGTC
Cuts at: 1864 1864
Size: 4067
XbaI T'CTAG_A
Cuts at: 495 1098 495
Size: 603 3464
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 955 1562 955
Size: 607 3460
XmaI C'CCGG_G
Cuts at: 1087 1087
Size: 4067
XmnI GAAnn'nnTTC
Cuts at: 609 609
Size: 4067
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbsI BcgI BfrBI BglI BmgBI BmtI
BpmI BsaXI BsgI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI