Restriction map for
pF1KB6447
(Circular) (Six-base) MAPSORT of: pF1KB6447.seq Check: 3873 from: 1 to: 3839
pF1KB6447 3839 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 877 877
Size: 3839
Acc65I G'GTAC_C
Cuts at: 855 855
Size: 3839
AclI AA'CG_TT
Cuts at: 3679 3679
Size: 3839
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 565 727 1664 2096 2843 3111 385
Size: 180 162 937 432 747 268 1113
Fragments arranged by size:
1113 937 747 432 268 180 162
AflIII A'CryG_T
Cuts at: 1191 2295 3272 1191
Size: 1104 977 1758
Fragments arranged by size:
1758 1104 977
AgeI A'CCGG_T
Cuts at: 779 2227 779
Size: 1448 2391
AlwNI CAG_nnn'CTG
Cuts at: 480 531 1144 2711 480
Size: 51 613 1567 1608
Fragments arranged by size:
1608 1567 613 51
ApaLI G'TGCA_C
Cuts at: 2609 2609
Size: 3839
ApoI r'AATT_y
Cuts at: 843 3192 3448 843
Size: 2349 256 1234
Fragments arranged by size:
2349 1234 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3839
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3839
AvaI C'yCGr_G
Cuts at: 859 859
Size: 3839
BamHI G'GATC_C
Cuts at: 419 864 419
Size: 445 3394
BanI G'GyrC_C
Cuts at: 655 855 1269 1517 1552 655
Size: 200 414 248 35 2942
Fragments arranged by size:
2942 414 248 200 35
BanII G_rGCy'C
Cuts at: 853 1883 853
Size: 1030 2809
BbeI G_GCGC'C
Cuts at: 1273 1521 1273
Size: 248 3591
BbsI GAAGACnn'nnnn_
Cuts at: 140 646 140
Size: 506 3333
BciVI GTATCCnnnnn_n'
Cuts at: 1737 2498 3839 1737
Size: 761 1341 1737
Fragments arranged by size:
1737 1341 761
BclI T'GATC_A
Cuts at: 1359 1359
Size: 3839
BfrBI ATG'CAT
Cuts at: 270 270
Size: 3839
BglII A'GATC_T
Cuts at: 1354 3391 1354
Size: 2037 1802
BlpI GC'TnA_GC
Cuts at: 532 951 532
Size: 419 3420
Bme1580I G_kGCm'C
Cuts at: 1464 1557 2613 1464
Size: 93 1056 2690
Fragments arranged by size:
2690 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 352 1051 1132 1467 3611 352
Size: 699 81 335 2144 580
Fragments arranged by size:
2144 699 580 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 3839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 262 538 751 172
Size: 90 276 213 3260
Fragments arranged by size:
3260 276 213 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 1015 1894 2386 2684 442
Size: 573 879 492 298 1597
Fragments arranged by size:
1597 879 573 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3543 3543
Size: 3839
BsaAI yAC'GTr
Cuts at: 1192 1192
Size: 3839
BsaBI GATnn'nnATC
Cuts at: 6 1353 3390 6
Size: 1347 2037 455
Fragments arranged by size:
2037 1347 455
BsaHI Gr'CG_yC
Cuts at: 214 1270 1518 2220 3716 214
Size: 1056 248 702 1496 337
Fragments arranged by size:
1496 1056 702 337 248
BsaWI w'CCGG_w
Cuts at: 779 827 1028 1549 2227 2501 2648 3059 779
Size: 48 201 521 678 274 147 411 1559
Fragments arranged by size:
1559 678 521 411 274 201 147 48
BseYI C'CCAG_C
Cuts at: 332 608 1264 2022 2599 3095 332
Size: 276 656 758 577 496 1076
Fragments arranged by size:
1076 758 656 577 496 276
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 304
Size: 3839
BsiEI CG_ry'CG
Cuts at: 104 784 1381 1427 2635 104
Size: 680 597 46 1208 1308
Fragments arranged by size:
1308 1208 680 597 46
BsiHKAI G_wGCw'C
Cuts at: 524 853 1631 1821 2613 524
Size: 329 778 190 792 1750
Fragments arranged by size:
1750 792 778 329 190
Bsp1286I G_dGCh'C
Cuts at: 524 853 1464 1557 1631 1821 1883 2613 524
Size: 329 611 93 74 190 62 730 1750
Fragments arranged by size:
1750 730 611 329 190 93 74 62
BspEI T'CCGG_A
Cuts at: 827 3059 827
Size: 2232 1607
BspHI T'CATG_A
Cuts at: 3015 3015
Size: 3839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 335 889 1103 1405 1786 335
Size: 554 214 302 381 2388
Fragments arranged by size:
2388 554 381 302 214
BsrBI CCG'CTC
Cuts at: 50 171 2131 2185 3669 3834 50
Size: 121 1960 54 1484 165 55
Fragments arranged by size:
1960 1484 165 121 55 54
BsrDI GCAATG_nn'
Cuts at: 1751 1751
Size: 3839
BsrFI r'CCGG_y
Cuts at: 779 1837 2018 2227 779
Size: 1058 181 209 2391
Fragments arranged by size:
2391 1058 209 181
BssSI C'ACGA_G
Cuts at: 2110 2468 2110
Size: 358 3481
BstAPI GCAn_nnn'nTGC
Cuts at: 1216 1216
Size: 3839
BstBI TT'CG_AA
Cuts at: 2200 2200
Size: 3839
BstXI CCAn_nnnn'nTGG
Cuts at: 114 233 666 114
Size: 119 433 3287
Fragments arranged by size:
3287 433 119
BstYI r'GATC_y
Cuts at: 1 419 864 1331 1354 1689 1935 2947 3033
Size: 418 445 467 23 335 246 1012 86
Cuts at: 3033 3045 3391 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 445 418 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1789 1970 2133 1789
Size: 181 163 3495
Fragments arranged by size:
3495 181 163
BtsI GCAGTG_nn'
Cuts at: 1082 1082
Size: 3839
DraI TTT'AAA
Cuts at: 838 838
Size: 3839
DrdI GACnn_nn'nnGTC
Cuts at: 1545 2403 1545
Size: 858 2981
EaeI y'GGCC_r
Cuts at: 140 668 1424 1598 1989 2016 3361 140
Size: 528 756 174 391 27 1345 618
Fragments arranged by size:
1345 756 618 528 391 174 27
EagI C'GGCC_G
Cuts at: 1424 1424
Size: 3839
EarI CTCTTCn'nnn_
Cuts at: 122 688 1862 2072 122
Size: 566 1174 210 1889
Fragments arranged by size:
1889 1174 566 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2047 2357 2503 3649 2047
Size: 310 146 1146 2237
Fragments arranged by size:
2237 1146 310 146
EcoICRI GAG'CTC
Cuts at: 851 851
Size: 3839
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 262 385 538 565 727 751 1664 2096
Size: 90 123 153 27 162 24 913 432
Cuts at: 2096 2843 3111 172
Size: 747 268 900
Fragments arranged by size:
913 900 747 432 268 162 153 123
90 27 24
EcoNI CCTnn'n_nnAGG
Cuts at: 577 709 577
Size: 132 3707
EcoO109I rG'GnC_Cy
Cuts at: 978 978
Size: 3839
EcoRI G'AATT_C
Cuts at: 843 843
Size: 3839
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1858 1858
Size: 3839
FspI TGC'GCA
Cuts at: 1620 1620
Size: 3839
HaeII r_GCGC'y
Cuts at: 511 539 1273 1521 2543 3522 511
Size: 28 734 248 1022 979 828
Fragments arranged by size:
1022 979 828 734 248 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 199 211 231 243 1681 1711 1713 1743 199
Size: 12 20 12 1438 30 2 30 2295
Fragments arranged by size:
2295 1438 30 30 20 12 12 2
HincII GTy'rAC
Cuts at: 878 878
Size: 3839
KasI G'GCGC_C
Cuts at: 1269 1517 1269
Size: 248 3591
KpnI G_GTAC'C
Cuts at: 859 859
Size: 3839
MluI A'CGCG_T
Cuts at: 3272 3272
Size: 3839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 197 764 2510 2694 197
Size: 567 1746 184 1342
Fragments arranged by size:
1746 1342 567 184
MscI TGG'CCA
Cuts at: 670 1600 670
Size: 930 2909
MslI CAynn'nnrTG
Cuts at: 664 1828 1955 664
Size: 1164 127 2548
Fragments arranged by size:
2548 1164 127
MspA1I CmG'CkG
Cuts at: 528 750 950 1264 1624 2637 2882 528
Size: 222 200 314 360 1013 245 1485
Fragments arranged by size:
1485 1013 360 314 245 222 200
NaeI GCC'GGC
Cuts at: 2020 2020
Size: 3839
NarI GG'CG_CC
Cuts at: 1270 1518 1270
Size: 248 3591
NgoMIV G'CCGG_C
Cuts at: 2018 2018
Size: 3839
NsiI A_TGCA'T
Cuts at: 272 272
Size: 3839
NspI r_CATG'y
Cuts at: 474 892 1392 2299 474
Size: 418 500 907 2014
Fragments arranged by size:
2014 907 500 418
PciI A'CATG_T
Cuts at: 2295 2295
Size: 3839
PfoI T'CCnGG_A
Cuts at: 398 398
Size: 3839
PmeI GTTT'AAAC
Cuts at: 838 838
Size: 3839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2978 3010 2978
Size: 32 3807
PstI C_TGCA'G
Cuts at: 161 182 886 1571 161
Size: 21 704 685 2429
Fragments arranged by size:
2429 704 685 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3839
PvuII CAG'CTG
Cuts at: 750 1264 1624 750
Size: 514 360 2965
Fragments arranged by size:
2965 514 360
RsrII CG'GwC_CG
Cuts at: 2034 2034
Size: 3839
SacI G_AGCT'C
Cuts at: 853 853
Size: 3839
SalI G'TCGA_C
Cuts at: 876 876
Size: 3839
SapI GCTCTTCn'nnn_
Cuts at: 1862 2072 1862
Size: 210 3629
SbfI CC_TGCA'GG
Cuts at: 886 886
Size: 3839
ScaI AGT'ACT
Cuts at: 148 3356 148
Size: 3208 631
SfcI C'TryA_G
Cuts at: 33 157 178 882 1567 2560 2751 33
Size: 124 21 704 685 993 191 1121
Fragments arranged by size:
1121 993 704 685 191 124 21
SfoI GGC'GCC
Cuts at: 1271 1519 1271
Size: 248 3591
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3839
SmaI CCC'GGG
Cuts at: 861 861
Size: 3839
SmlI C'TyrA_G
Cuts at: 457 994 1909 2401 2663 457
Size: 537 915 492 262 1633
Fragments arranged by size:
1633 915 537 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3839
SphI G_CATG'C
Cuts at: 892 1392 892
Size: 500 3339
StyI C'CwwG_G
Cuts at: 973 1327 973
Size: 354 3485
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 754 1422 1555 2014 2223 754
Size: 668 133 459 209 2370
Fragments arranged by size:
2370 668 459 209 133
TatI w'GTAC_w
Cuts at: 146 3354 146
Size: 3208 631
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3356 3388 3356
Size: 32 3807
Tth111I GACn'n_nGTC
Cuts at: 1636 1636
Size: 3839
XbaI T'CTAG_A
Cuts at: 870 870
Size: 3839
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 237 1334 237
Size: 1097 2742
XmaI C'CCGG_G
Cuts at: 859 859
Size: 3839
XmnI GAAnn'nnTTC
Cuts at: 682 682
Size: 3839
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI