Restriction map for
pF1KB6441
(Circular) (Six-base) MAPSORT of: pF1KB6441.seq Check: 4134 from: 1 to: 4055
pF1KB6441 4055 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 1093 1093
Size: 4055
Acc65I G'GTAC_C
Cuts at: 1071 1071
Size: 4055
AclI AA'CG_TT
Cuts at: 3895 3895
Size: 4055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 1880 2312 3059 3327 449
Size: 1431 432 747 268 1177
Fragments arranged by size:
1431 1177 747 432 268
AflIII A'CryG_T
Cuts at: 929 1407 2511 3488 929
Size: 478 1104 977 1496
Fragments arranged by size:
1496 1104 977 478
AgeI A'CCGG_T
Cuts at: 2443 2443
Size: 4055
AhdI GACnn_n'nnGTC
Cuts at: 369 369
Size: 4055
AlwNI CAG_nnn'CTG
Cuts at: 1360 2927 1360
Size: 1567 2488
ApaLI G'TGCA_C
Cuts at: 2825 2825
Size: 4055
ApoI r'AATT_y
Cuts at: 907 1059 3408 3664 907
Size: 152 2349 256 1298
Fragments arranged by size:
2349 1298 256 152
AseI AT'TA_AT
Cuts at: 20 20
Size: 4055
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
AvaI C'yCGr_G
Cuts at: 1075 1075
Size: 4055
BamHI G'GATC_C
Cuts at: 294 1080 294
Size: 786 3269
BanI G'GyrC_C
Cuts at: 1071 1485 1733 1768 1071
Size: 414 248 35 3358
Fragments arranged by size:
3358 414 248 35
BanII G_rGCy'C
Cuts at: 1069 2099 1069
Size: 1030 3025
BbeI G_GCGC'C
Cuts at: 1489 1737 1489
Size: 248 3807
BciVI GTATCCnnnnn_n'
Cuts at: 1953 2714 4055 1953
Size: 761 1341 1953
Fragments arranged by size:
1953 1341 761
BclI T'GATC_A
Cuts at: 1005 1575 1005
Size: 570 3485
BfrBI ATG'CAT
Cuts at: 992 992
Size: 4055
BglII A'GATC_T
Cuts at: 1570 3607 1570
Size: 2037 2018
BlpI GC'TnA_GC
Cuts at: 1167 1167
Size: 4055
Bme1580I G_kGCm'C
Cuts at: 1680 1773 2829 1680
Size: 93 1056 2906
Fragments arranged by size:
2906 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 764 1267 1348 1683 3827 764
Size: 503 81 335 2144 992
Fragments arranged by size:
2144 992 503 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 257
Size: 4055
Bpu10I CC'TnA_GC
Cuts at: 784 784
Size: 4055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1231 2110 2602 2900 1231
Size: 879 492 298 2386
Fragments arranged by size:
2386 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3759 3759
Size: 4055
BsaAI yAC'GTr
Cuts at: 930 1408 930
Size: 478 3577
BsaBI GATnn'nnATC
Cuts at: 6 1569 3606 6
Size: 1563 2037 455
Fragments arranged by size:
2037 1563 455
BsaHI Gr'CG_yC
Cuts at: 1486 1734 2436 3932 1486
Size: 248 702 1496 1609
Fragments arranged by size:
1609 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1244 1765 2443 2717 2864 3275 1244
Size: 521 678 274 147 411 2024
Fragments arranged by size:
2024 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1480 2238 2815 3311 1480
Size: 758 577 496 2224
Fragments arranged by size:
2224 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1597 1643 2851 104
Size: 1493 46 1208 1308
Fragments arranged by size:
1493 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1069 1847 2037 2829 1069
Size: 778 190 792 2295
Fragments arranged by size:
2295 792 778 190
BsmI GAATG_Cn'
Cuts at: 994 994
Size: 4055
Bsp1286I G_dGCh'C
Cuts at: 1069 1680 1773 1847 2037 2099 2829 1069
Size: 611 93 74 190 62 730 2295
Fragments arranged by size:
2295 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3275 3275
Size: 4055
BspHI T'CATG_A
Cuts at: 3231 3231
Size: 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1105 1319 1621 2002 1105
Size: 214 302 381 3158
Fragments arranged by size:
3158 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2347 2401 3885 4050 50
Size: 2297 54 1484 165 55
Fragments arranged by size:
2297 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1967 1967
Size: 4055
BsrFI r'CCGG_y
Cuts at: 2053 2234 2443 2053
Size: 181 209 3665
Fragments arranged by size:
3665 209 181
BsrGI T'GTAC_A
Cuts at: 846 846
Size: 4055
BssSI C'ACGA_G
Cuts at: 2326 2684 2326
Size: 358 3697
BstAPI GCAn_nnn'nTGC
Cuts at: 1432 1432
Size: 4055
BstBI TT'CG_AA
Cuts at: 2416 2416
Size: 4055
BstYI r'GATC_y
Cuts at: 1 294 1080 1547 1570 1905 2151 3163 3249
Size: 293 786 467 23 335 246 1012 86
Cuts at: 3249 3261 3607 1
Size: 12 346 449
Fragments arranged by size:
1012 786 467 449 346 335 293 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 573 965 107
Size: 466 392 3197
Fragments arranged by size:
3197 466 392
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2005 2186 2349 2005
Size: 181 163 3711
Fragments arranged by size:
3711 181 163
BtsI GCAGTG_nn'
Cuts at: 1298 1298
Size: 4055
DraI TTT'AAA
Cuts at: 681 740 1054 681
Size: 59 314 3682
Fragments arranged by size:
3682 314 59
DrdI GACnn_nn'nnGTC
Cuts at: 1761 2619 1761
Size: 858 3197
EaeI y'GGCC_r
Cuts at: 1640 1814 2205 2232 3577 1640
Size: 174 391 27 1345 2118
Fragments arranged by size:
2118 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1640 1640
Size: 4055
EarI CTCTTCn'nnn_
Cuts at: 2078 2288 2078
Size: 210 3845
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2263 2573 2719 3865 2263
Size: 310 146 1146 2453
Fragments arranged by size:
2453 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1067 1067
Size: 4055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 449 1880 2312 3059 3327 257
Size: 192 1431 432 747 268 985
Fragments arranged by size:
1431 985 747 432 268 192
EcoO109I rG'GnC_Cy
Cuts at: 1194 1194
Size: 4055
EcoRI G'AATT_C
Cuts at: 1059 1059
Size: 4055
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 424 2074 424
Size: 1650 2405
FspI TGC'GCA
Cuts at: 1836 1836
Size: 4055
HaeII r_GCGC'y
Cuts at: 1489 1737 2759 3738 1489
Size: 248 1022 979 1806
Fragments arranged by size:
1806 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 279 311 355 387 657 689 1897 1927 1929
Size: 32 44 32 270 32 1208 30 2
Cuts at: 1929 1959 279
Size: 30 2375
Fragments arranged by size:
2375 1208 270 44 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1094 1094
Size: 4055
KasI G'GCGC_C
Cuts at: 1485 1733 1485
Size: 248 3807
KpnI G_GTAC'C
Cuts at: 1075 1075
Size: 4055
MfeI C'AATT_G
Cuts at: 545 545
Size: 4055
MluI A'CGCG_T
Cuts at: 3488 3488
Size: 4055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2726 2910 2726
Size: 184 3871
MscI TGG'CCA
Cuts at: 1816 1816
Size: 4055
MslI CAynn'nnrTG
Cuts at: 610 2044 2171 610
Size: 1434 127 2494
Fragments arranged by size:
2494 1434 127
MspA1I CmG'CkG
Cuts at: 1166 1480 1840 2853 3098 1166
Size: 314 360 1013 245 2123
Fragments arranged by size:
2123 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2236 2236
Size: 4055
NarI GG'CG_CC
Cuts at: 1486 1734 1486
Size: 248 3807
NcoI C'CATG_G
Cuts at: 107 573 965 107
Size: 466 392 3197
Fragments arranged by size:
3197 466 392
NgoMIV G'CCGG_C
Cuts at: 2234 2234
Size: 4055
NsiI A_TGCA'T
Cuts at: 994 994
Size: 4055
NspI r_CATG'y
Cuts at: 1108 1608 2515 1108
Size: 500 907 2648
Fragments arranged by size:
2648 907 500
PciI A'CATG_T
Cuts at: 2511 2511
Size: 4055
PmeI GTTT'AAAC
Cuts at: 1054 1054
Size: 4055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3194 3226 3194
Size: 32 4023
PstI C_TGCA'G
Cuts at: 1102 1787 1102
Size: 685 3370
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4055
PvuII CAG'CTG
Cuts at: 1480 1840 1480
Size: 360 3695
RsrII CG'GwC_CG
Cuts at: 2250 2250
Size: 4055
SacI G_AGCT'C
Cuts at: 1069 1069
Size: 4055
SalI G'TCGA_C
Cuts at: 1092 1092
Size: 4055
SapI GCTCTTCn'nnn_
Cuts at: 2078 2288 2078
Size: 210 3845
SbfI CC_TGCA'GG
Cuts at: 1102 1102
Size: 4055
ScaI AGT'ACT
Cuts at: 3572 3572
Size: 4055
SfcI C'TryA_G
Cuts at: 33 654 1098 1783 2776 2967 33
Size: 621 444 685 993 191 1121
Fragments arranged by size:
1121 993 685 621 444 191
SfoI GGC'GCC
Cuts at: 1487 1735 1487
Size: 248 3807
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
SmaI CCC'GGG
Cuts at: 1077 1077
Size: 4055
SmlI C'TyrA_G
Cuts at: 1210 2125 2617 2879 1210
Size: 915 492 262 2386
Fragments arranged by size:
2386 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4055
SphI G_CATG'C
Cuts at: 1108 1608 1108
Size: 500 3555
StyI C'CwwG_G
Cuts at: 107 573 965 1189 1543 107
Size: 466 392 224 354 2619
Fragments arranged by size:
2619 466 392 354 224
SwaI ATTT'AAAT
Cuts at: 740 740
Size: 4055
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1638 1771 2230 2439 1638
Size: 133 459 209 3254
Fragments arranged by size:
3254 459 209 133
TatI w'GTAC_w
Cuts at: 846 3570 846
Size: 2724 1331
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 236 268 3572 3604 236
Size: 32 3304 32 687
Fragments arranged by size:
3304 687 32 32
Tth111I GACn'n_nGTC
Cuts at: 1852 1852
Size: 4055
XbaI T'CTAG_A
Cuts at: 1086 1086
Size: 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1550 114
Size: 1436 2619
XmaI C'CCGG_G
Cuts at: 1075 1075
Size: 4055
XmnI GAAnn'nnTTC
Cuts at: 149 149
Size: 4055
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI BsaXI BseRI BsgI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI XhoI ZraI