Restriction map for
pF1KB6434
(Circular) (Six-base) MAPSORT of: pF1KB6434.seq Check: 9885 from: 1 to: 4145
pF1KB6434 4145 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1183 1183
Size: 4145
Acc65I G'GTAC_C
Cuts at: 303 1161 303
Size: 858 3287
AclI AA'CG_TT
Cuts at: 3985 3985
Size: 4145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 795 964 1970 2402 3149 3417 795
Size: 169 1006 432 747 268 1523
Fragments arranged by size:
1523 1006 747 432 268 169
AflIII A'CryG_T
Cuts at: 1497 2601 3578 1497
Size: 1104 977 2064
Fragments arranged by size:
2064 1104 977
AgeI A'CCGG_T
Cuts at: 2533 2533
Size: 4145
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 988 1020 988
Size: 32 4113
AlwNI CAG_nnn'CTG
Cuts at: 1450 3017 1450
Size: 1567 2578
ApaLI G'TGCA_C
Cuts at: 2915 2915
Size: 4145
ApoI r'AATT_y
Cuts at: 1149 3498 3754 1149
Size: 2349 256 1540
Fragments arranged by size:
2349 1540 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4145
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
AvaI C'yCGr_G
Cuts at: 859 1165 859
Size: 306 3839
AvrII C'CTAG_G
Cuts at: 1059 1059
Size: 4145
BamHI G'GATC_C
Cuts at: 294 362 1170 294
Size: 68 808 3269
Fragments arranged by size:
3269 808 68
BanI G'GyrC_C
Cuts at: 303 687 1079 1161 1575 1823 1858 303
Size: 384 392 82 414 248 35 2590
Fragments arranged by size:
2590 414 392 384 248 82 35
BanII G_rGCy'C
Cuts at: 919 1159 2189 919
Size: 240 1030 2875
Fragments arranged by size:
2875 1030 240
BbeI G_GCGC'C
Cuts at: 1579 1827 1579
Size: 248 3897
BbvCI CC'TCA_GC
Cuts at: 379 1027 379
Size: 648 3497
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 825 859 825
Size: 34 4111
BciVI GTATCCnnnnn_n'
Cuts at: 698 2043 2804 4145 698
Size: 1345 761 1341 698
Fragments arranged by size:
1345 1341 761 698
BclI T'GATC_A
Cuts at: 1665 1665
Size: 4145
BglI GCCn_nnn'nGGC
Cuts at: 696 696
Size: 4145
BglII A'GATC_T
Cuts at: 1660 3697 1660
Size: 2037 2108
BlpI GC'TnA_GC
Cuts at: 779 1257 779
Size: 478 3667
Bme1580I G_kGCm'C
Cuts at: 1082 1770 1863 2919 1082
Size: 688 93 1056 2308
Fragments arranged by size:
2308 1056 688 93
BmrI ACTGGGnnnn_n'
Cuts at: 1357 1438 1773 3917 1357
Size: 81 335 2144 1585
Fragments arranged by size:
2144 1585 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 490 2512 490
Size: 2022 2123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 990 990
Size: 4145
Bpu10I CC'TnA_GC
Cuts at: 379 1027 379
Size: 648 3497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1321 2200 2692 2990 1321
Size: 879 492 298 2476
Fragments arranged by size:
2476 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 782 3849 782
Size: 3067 1078
BsaAI yAC'GTr
Cuts at: 1498 1498
Size: 4145
BsaBI GATnn'nnATC
Cuts at: 6 367 1659 3696 6
Size: 361 1292 2037 455
Fragments arranged by size:
2037 1292 455 361
BsaHI Gr'CG_yC
Cuts at: 1576 1824 2526 4022 1576
Size: 248 702 1496 1699
Fragments arranged by size:
1699 1496 702 248
BsaWI w'CCGG_w
Cuts at: 456 1334 1855 2533 2807 2954 3365 456
Size: 878 521 678 274 147 411 1236
Fragments arranged by size:
1236 878 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 988 1018 988
Size: 30 4115
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 388 629 1026 1075 388
Size: 241 397 49 3458
Fragments arranged by size:
3458 397 241 49
BseYI C'CCAG_C
Cuts at: 542 748 820 831 932 1570 2328 2905 3401
Size: 206 72 11 101 638 758 577 496
Cuts at: 3401 542
Size: 1286
Fragments arranged by size:
1286 758 638 577 496 206 101 72
11
BsiEI CG_ry'CG
Cuts at: 104 1687 1733 2941 104
Size: 1583 46 1208 1308
Fragments arranged by size:
1583 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 211 476 780 1159 1937 2127 2919 211
Size: 265 304 379 778 190 792 1437
Fragments arranged by size:
1437 792 778 379 304 265 190
Bsp1286I G_dGCh'C
Cuts at: 211 476 780 919 1082 1159 1770 1863 1937
Size: 265 304 139 163 77 611 93 74
Cuts at: 1937 2127 2189 2919 211
Size: 190 62 730 1437
Fragments arranged by size:
1437 730 611 304 265 190 163 139
93 77 74 62
BspEI T'CCGG_A
Cuts at: 3365 3365
Size: 4145
BspHI T'CATG_A
Cuts at: 642 3321 642
Size: 2679 1466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 731 1195 1409 1711 2092 731
Size: 464 214 302 381 2784
Fragments arranged by size:
2784 464 381 302 214
BsrBI CCG'CTC
Cuts at: 50 335 1042 2437 2491 3975 4140 50
Size: 285 707 1395 54 1484 165 55
Fragments arranged by size:
1484 1395 707 285 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1112 2057 1112
Size: 945 3200
BsrFI r'CCGG_y
Cuts at: 433 2143 2324 2533 433
Size: 1710 181 209 2045
Fragments arranged by size:
2045 1710 209 181
BssSI C'ACGA_G
Cuts at: 2416 2774 2416
Size: 358 3787
BstAPI GCAn_nnn'nTGC
Cuts at: 775 1522 775
Size: 747 3398
BstBI TT'CG_AA
Cuts at: 2506 2506
Size: 4145
BstYI r'GATC_y
Cuts at: 1 182 294 362 983 1170 1637 1660 1995
Size: 181 112 68 621 187 467 23 335
Cuts at: 1995 2241 3253 3339 3351 3697 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 621 467 449 346 335 246 187
181 112 86 68 23 12
BtgI C'CryG_G
Cuts at: 107 1091 107
Size: 984 3161
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 426 2095 2276 2439 426
Size: 1669 181 163 2132
Fragments arranged by size:
2132 1669 181 163
BtsI GCAGTG_nn'
Cuts at: 602 857 1388 602
Size: 255 531 3359
Fragments arranged by size:
3359 531 255
DraI TTT'AAA
Cuts at: 1144 1144
Size: 4145
DrdI GACnn_nn'nnGTC
Cuts at: 1851 2709 1851
Size: 858 3287
EaeI y'GGCC_r
Cuts at: 110 1730 1904 2295 2322 3667 110
Size: 1620 174 391 27 1345 588
Fragments arranged by size:
1620 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 1730 1730
Size: 4145
EarI CTCTTCn'nnn_
Cuts at: 922 2168 2378 922
Size: 1246 210 2689
Fragments arranged by size:
2689 1246 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2353 2663 2809 3955 2353
Size: 310 146 1146 2543
Fragments arranged by size:
2543 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1157 1157
Size: 4145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 795 964 990 1970 2402 3149 3417 795
Size: 169 26 980 432 747 268 1523
Fragments arranged by size:
1523 980 747 432 268 169 26
EcoNI CCTnn'n_nnAGG
Cuts at: 370 370
Size: 4145
EcoO109I rG'GnC_Cy
Cuts at: 156 1284 156
Size: 1128 3017
EcoRI G'AATT_C
Cuts at: 1149 1149
Size: 4145
EcoRV GAT'ATC
Cuts at: 648 648
Size: 4145
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 973 2164 973
Size: 1191 2954
FspI TGC'GCA
Cuts at: 1926 1926
Size: 4145
HaeII r_GCGC'y
Cuts at: 967 1106 1579 1827 2849 3828 967
Size: 139 473 248 1022 979 1284
Fragments arranged by size:
1284 1022 979 473 248 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 140 172 696 728 887 919 975 1007 1987
Size: 32 524 32 159 32 56 32 980
Cuts at: 1987 2017 2019 2049 140
Size: 30 2 30 2236
Fragments arranged by size:
2236 980 524 159 56 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1184 1184
Size: 4145
HindIII A'AGCT_T
Cuts at: 315 315
Size: 4145
KasI G'GCGC_C
Cuts at: 1575 1823 1575
Size: 248 3897
KpnI G_GTAC'C
Cuts at: 307 1165 307
Size: 858 3287
MluI A'CGCG_T
Cuts at: 3578 3578
Size: 4145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 518 2816 3000 518
Size: 2298 184 1663
Fragments arranged by size:
2298 1663 184
MscI TGG'CCA
Cuts at: 1906 1906
Size: 4145
MslI CAynn'nnrTG
Cuts at: 1018 2134 2261 1018
Size: 1116 127 2902
Fragments arranged by size:
2902 1116 127
MspA1I CmG'CkG
Cuts at: 309 1256 1570 1930 2943 3188 309
Size: 947 314 360 1013 245 1266
Fragments arranged by size:
1266 1013 947 360 314 245
NaeI GCC'GGC
Cuts at: 435 2326 435
Size: 1891 2254
NarI GG'CG_CC
Cuts at: 1576 1824 1576
Size: 248 3897
NcoI C'CATG_G
Cuts at: 107 1091 107
Size: 984 3161
NdeI CA'TA_TG
Cuts at: 551 551
Size: 4145
NgoMIV G'CCGG_C
Cuts at: 433 2324 433
Size: 1891 2254
NspI r_CATG'y
Cuts at: 1198 1698 2605 1198
Size: 500 907 2738
Fragments arranged by size:
2738 907 500
PasI CC'CwG_GG
Cuts at: 972 972
Size: 4145
PciI A'CATG_T
Cuts at: 2601 2601
Size: 4145
PflMI CCAn_nnn'nTGG
Cuts at: 555 555
Size: 4145
PmeI GTTT'AAAC
Cuts at: 1144 1144
Size: 4145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 988 1020 3284 3316 988
Size: 32 2264 32 1817
Fragments arranged by size:
2264 1817 32 32
PpuMI rG'GwC_Cy
Cuts at: 156 156
Size: 4145
PstI C_TGCA'G
Cuts at: 502 1192 1877 502
Size: 690 685 2770
Fragments arranged by size:
2770 690 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4145
PvuII CAG'CTG
Cuts at: 1570 1930 1570
Size: 360 3785
RsrII CG'GwC_CG
Cuts at: 2340 2340
Size: 4145
SacI G_AGCT'C
Cuts at: 1159 1159
Size: 4145
SalI G'TCGA_C
Cuts at: 1182 1182
Size: 4145
SapI GCTCTTCn'nnn_
Cuts at: 922 2168 2378 922
Size: 1246 210 2689
Fragments arranged by size:
2689 1246 210
SbfI CC_TGCA'GG
Cuts at: 1192 1192
Size: 4145
ScaI AGT'ACT
Cuts at: 3662 3662
Size: 4145
SfcI C'TryA_G
Cuts at: 33 498 1098 1188 1873 2866 3057 33
Size: 465 600 90 685 993 191 1121
Fragments arranged by size:
1121 993 685 600 465 191 90
SfoI GGC'GCC
Cuts at: 1577 1825 1577
Size: 248 3897
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
SmaI CCC'GGG
Cuts at: 1167 1167
Size: 4145
SmlI C'TyrA_G
Cuts at: 1300 2215 2707 2969 1300
Size: 915 492 262 2476
Fragments arranged by size:
2476 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4145
SphI G_CATG'C
Cuts at: 1198 1698 1198
Size: 500 3645
StyI C'CwwG_G
Cuts at: 107 1059 1091 1279 1633 107
Size: 952 32 188 354 2619
Fragments arranged by size:
2619 952 354 188 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 401 547 1036 1728 1861 2320 2529 401
Size: 146 489 692 133 459 209 2017
Fragments arranged by size:
2017 692 489 459 209 146 133
TatI w'GTAC_w
Cuts at: 3660 3660
Size: 4145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3662 3694 3662
Size: 32 4113
Tth111I GACn'n_nGTC
Cuts at: 871 1942 871
Size: 1071 3074
XbaI T'CTAG_A
Cuts at: 1176 1176
Size: 4145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 889 909 1640 889
Size: 20 731 3394
Fragments arranged by size:
3394 731 20
XmaI C'CCGG_G
Cuts at: 1165 1165
Size: 4145
XmnI GAAnn'nnTTC
Cuts at: 953 1000 953
Size: 47 4098
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI BaeI BbsI BfrBI BmgBI BmtI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII FseI FspAI HpaI MfeI NheI
NotI NruI NsiI PacI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI