(Circular) (Six-base) MAPSORT of: pF1KB6432.seq Check: 4864 from: 1 to: 4145 pF1KB6432 4145 bp With 182 enzymes: SgfI * June 26, 2008 10:47 .. AccI GT'mk_AC Cuts at: 1183 1183 Size: 4145 Acc65I G'GTAC_C Cuts at: 1161 1161 Size: 4145 AclI AA'CG_TT Cuts at: 3985 3985 Size: 4145 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 358 806 1970 2402 3149 3417 358 Size: 448 1164 432 747 268 1086 Fragments arranged by size: 1164 1086 747 448 432 268 AflIII A'CryG_T Cuts at: 1497 2601 3578 1497 Size: 1104 977 2064 Fragments arranged by size: 2064 1104 977 AgeI A'CCGG_T Cuts at: 2533 2533 Size: 4145 AhdI GACnn_n'nnGTC Cuts at: 899 899 Size: 4145 AlwNI CAG_nnn'CTG Cuts at: 1450 3017 1450 Size: 1567 2578 ApaLI G'TGCA_C Cuts at: 326 2915 326 Size: 2589 1556 ApoI r'AATT_y Cuts at: 460 649 791 1149 3498 3754 460 Size: 189 142 358 2349 256 851 Fragments arranged by size: 2349 851 358 256 189 142 AseI AT'TA_AT Cuts at: 20 20 Size: 4145 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4145 AvaI C'yCGr_G Cuts at: 135 185 1165 135 Size: 50 980 3115 Fragments arranged by size: 3115 980 50 BamHI G'GATC_C Cuts at: 1170 1170 Size: 4145 BanI G'GyrC_C Cuts at: 1161 1575 1823 1858 1161 Size: 414 248 35 3448 Fragments arranged by size: 3448 414 248 35 BanII G_rGCy'C Cuts at: 1159 2189 1159 Size: 1030 3115 BbeI G_GCGC'C Cuts at: 1579 1827 1579 Size: 248 3897 BciVI GTATCCnnnnn_n' Cuts at: 2043 2804 4145 2043 Size: 761 1341 2043 Fragments arranged by size: 2043 1341 761 BclI T'GATC_A Cuts at: 1665 1665 Size: 4145 BglII A'GATC_T Cuts at: 1660 3697 1660 Size: 2037 2108 BlpI GC'TnA_GC Cuts at: 1257 1257 Size: 4145 Bme1580I G_kGCm'C Cuts at: 330 1077 1770 1863 2919 330 Size: 747 693 93 1056 1556 Fragments arranged by size: 1556 1056 747 693 93 BmrI ACTGGGnnnn_n' Cuts at: 1357 1438 1773 3917 1357 Size: 81 335 2144 1585 Fragments arranged by size: 2144 1585 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 211 2512 211 Size: 2301 1844 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 178 271 178 Size: 93 4052 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 961 1321 2200 2692 2990 961 Size: 360 879 492 298 2116 Fragments arranged by size: 2116 879 492 360 298 BsaI GGTCTCn'nnnn_ Cuts at: 3849 3849 Size: 4145 BsaAI yAC'GTr Cuts at: 738 1498 738 Size: 760 3385 BsaBI GATnn'nnATC Cuts at: 6 671 1659 3696 6 Size: 665 988 2037 455 Fragments arranged by size: 2037 988 665 455 BsaHI Gr'CG_yC Cuts at: 1576 1824 2526 4022 1576 Size: 248 702 1496 1699 Fragments arranged by size: 1699 1496 702 248 BsaWI w'CCGG_w Cuts at: 1334 1855 2533 2807 2954 3365 1334 Size: 521 678 274 147 411 2114 Fragments arranged by size: 2114 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1073 1103 1073 Size: 30 4115 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 406 1081 406 Size: 675 3470 BseYI C'CCAG_C Cuts at: 1570 2328 2905 3401 1570 Size: 758 577 496 2314 Fragments arranged by size: 2314 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1687 1733 2941 104 Size: 1583 46 1208 1308 Fragments arranged by size: 1583 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 197 330 1159 1937 2127 2919 197 Size: 133 829 778 190 792 1423 Fragments arranged by size: 1423 829 792 778 190 133 BsmI GAATG_Cn' Cuts at: 301 301 Size: 4145 Bsp1286I G_dGCh'C Cuts at: 197 330 1077 1159 1770 1863 1937 2127 2189 Size: 133 747 82 611 93 74 190 62 Cuts at: 2189 2919 197 Size: 730 1423 Fragments arranged by size: 1423 747 730 611 190 133 93 82 74 62 BspEI T'CCGG_A Cuts at: 3365 3365 Size: 4145 BspHI T'CATG_A Cuts at: 3321 3321 Size: 4145 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1195 1409 1711 2092 1195 Size: 214 302 381 3248 Fragments arranged by size: 3248 381 302 214 BsrBI CCG'CTC Cuts at: 50 2437 2491 3975 4140 50 Size: 2387 54 1484 165 55 Fragments arranged by size: 2387 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 313 663 2057 313 Size: 350 1394 2401 Fragments arranged by size: 2401 1394 350 BsrFI r'CCGG_y Cuts at: 2143 2324 2533 2143 Size: 181 209 3755 Fragments arranged by size: 3755 209 181 BssSI C'ACGA_G Cuts at: 2416 2774 2416 Size: 358 3787 BstAPI GCAn_nnn'nTGC Cuts at: 1522 1522 Size: 4145 BstBI TT'CG_AA Cuts at: 2506 2506 Size: 4145 BstYI r'GATC_y Cuts at: 1 533 1170 1637 1660 1995 2241 3253 3339 Size: 532 637 467 23 335 246 1012 86 Cuts at: 3339 3351 3697 1 Size: 12 346 449 Fragments arranged by size: 1012 637 532 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 119 1118 107 Size: 12 999 3134 Fragments arranged by size: 3134 999 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2095 2276 2439 2095 Size: 181 163 3801 Fragments arranged by size: 3801 181 163 BtsI GCAGTG_nn' Cuts at: 1388 1388 Size: 4145 ClaI AT'CG_AT Cuts at: 272 272 Size: 4145 DraI TTT'AAA Cuts at: 729 1144 729 Size: 415 3730 DrdI GACnn_nn'nnGTC Cuts at: 1851 2709 1851 Size: 858 3287 EaeI y'GGCC_r Cuts at: 1730 1904 2295 2322 3667 1730 Size: 174 391 27 1345 2208 Fragments arranged by size: 2208 1345 391 174 27 EagI C'GGCC_G Cuts at: 1730 1730 Size: 4145 EarI CTCTTCn'nnn_ Cuts at: 407 2168 2378 407 Size: 1761 210 2174 Fragments arranged by size: 2174 1761 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2353 2663 2809 3955 2353 Size: 310 146 1146 2543 Fragments arranged by size: 2543 1146 310 146 EcoICRI GAG'CTC Cuts at: 1157 1157 Size: 4145 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 178 271 358 806 1970 2402 3149 3417 178 Size: 93 87 448 1164 432 747 268 906 Fragments arranged by size: 1164 906 747 448 432 268 93 87 EcoNI CCTnn'n_nnAGG Cuts at: 481 481 Size: 4145 EcoO109I rG'GnC_Cy Cuts at: 1284 1284 Size: 4145 EcoRI G'AATT_C Cuts at: 791 1149 791 Size: 358 3787 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2164 2164 Size: 4145 FspI TGC'GCA Cuts at: 1926 1926 Size: 4145 HaeII r_GCGC'y Cuts at: 1579 1827 2849 3828 1579 Size: 248 1022 979 1896 Fragments arranged by size: 1896 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 885 917 1987 2017 2019 2049 885 Size: 32 1070 30 2 30 2981 Fragments arranged by size: 2981 1070 32 30 30 2 HincII GTy'rAC Cuts at: 1184 1184 Size: 4145 HindIII A'AGCT_T Cuts at: 996 996 Size: 4145 KasI G'GCGC_C Cuts at: 1575 1823 1575 Size: 248 3897 KpnI G_GTAC'C Cuts at: 1165 1165 Size: 4145 MluI A'CGCG_T Cuts at: 3578 3578 Size: 4145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 210 2816 3000 210 Size: 2606 184 1355 Fragments arranged by size: 2606 1355 184 MscI TGG'CCA Cuts at: 1906 1906 Size: 4145 MslI CAynn'nnrTG Cuts at: 2134 2261 2134 Size: 127 4018 MspA1I CmG'CkG Cuts at: 143 1256 1570 1930 2943 3188 143 Size: 1113 314 360 1013 245 1100 Fragments arranged by size: 1113 1100 1013 360 314 245 NaeI GCC'GGC Cuts at: 2326 2326 Size: 4145 NarI GG'CG_CC Cuts at: 1576 1824 1576 Size: 248 3897 NcoI C'CATG_G Cuts at: 107 1118 107 Size: 1011 3134 NgoMIV G'CCGG_C Cuts at: 2324 2324 Size: 4145 NspI r_CATG'y Cuts at: 825 1198 1698 2605 825 Size: 373 500 907 2365 Fragments arranged by size: 2365 907 500 373 PciI A'CATG_T Cuts at: 2601 2601 Size: 4145 PmeI GTTT'AAAC Cuts at: 1144 1144 Size: 4145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3284 3316 3284 Size: 32 4113 PstI C_TGCA'G Cuts at: 1192 1877 1192 Size: 685 3460 PvuI CG_AT'CG Cuts at: 104 104 Size: 4145 PvuII CAG'CTG Cuts at: 1570 1930 1570 Size: 360 3785 RsrII CG'GwC_CG Cuts at: 2340 2340 Size: 4145 SacI G_AGCT'C Cuts at: 1159 1159 Size: 4145 SalI G'TCGA_C Cuts at: 1182 1182 Size: 4145 SapI GCTCTTCn'nnn_ Cuts at: 2168 2378 2168 Size: 210 3935 SbfI CC_TGCA'GG Cuts at: 1192 1192 Size: 4145 ScaI AGT'ACT Cuts at: 3662 3662 Size: 4145 SfcI C'TryA_G Cuts at: 33 848 1188 1873 2866 3057 33 Size: 815 340 685 993 191 1121 Fragments arranged by size: 1121 993 815 685 340 191 SfoI GGC'GCC Cuts at: 1577 1825 1577 Size: 248 3897 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4145 SmaI CCC'GGG Cuts at: 1167 1167 Size: 4145 SmlI C'TyrA_G Cuts at: 940 1300 2215 2707 2969 940 Size: 360 915 492 262 2116 Fragments arranged by size: 2116 915 492 360 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4145 SphI G_CATG'C Cuts at: 825 1198 1698 825 Size: 373 500 3272 Fragments arranged by size: 3272 500 373 SspI AAT'ATT Cuts at: 870 982 870 Size: 112 4033 StyI C'CwwG_G Cuts at: 107 1118 1279 1633 107 Size: 1011 161 354 2619 Fragments arranged by size: 2619 1011 354 161 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1728 1861 2320 2529 1728 Size: 133 459 209 3344 Fragments arranged by size: 3344 459 209 133 TatI w'GTAC_w Cuts at: 3660 3660 Size: 4145 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3662 3694 3662 Size: 32 4113 Tth111I GACn'n_nGTC Cuts at: 1942 1942 Size: 4145 XbaI T'CTAG_A Cuts at: 748 1176 748 Size: 428 3717 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1640 1640 Size: 4145 XmaI C'CCGG_G Cuts at: 1165 1165 Size: 4145 XmnI GAAnn'nnTTC Cuts at: 206 554 791 983 206 Size: 348 237 192 3368 Fragments arranged by size: 3368 348 237 192 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI