Restriction map for
pF1KB6426
(Circular) (Six-base) MAPSORT of: pF1KB6426.seq Check: 1369 from: 1 to: 3971
pF1KB6426 3971 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1009 1009
Size: 3971
Acc65I G'GTAC_C
Cuts at: 987 987
Size: 3971
AclI AA'CG_TT
Cuts at: 3811 3811
Size: 3971
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1796 2228 2975 3243 1796
Size: 432 747 268 2524
Fragments arranged by size:
2524 747 432 268
AflIII A'CryG_T
Cuts at: 1323 2427 3404 1323
Size: 1104 977 1890
Fragments arranged by size:
1890 1104 977
AgeI A'CCGG_T
Cuts at: 2359 2359
Size: 3971
AhdI GACnn_n'nnGTC
Cuts at: 516 516
Size: 3971
AleI CACnn'nnGTG
Cuts at: 353 556 353
Size: 203 3768
AlwNI CAG_nnn'CTG
Cuts at: 573 1276 2843 573
Size: 703 1567 1701
Fragments arranged by size:
1701 1567 703
ApaLI G'TGCA_C
Cuts at: 2741 2741
Size: 3971
ApoI r'AATT_y
Cuts at: 975 3324 3580 975
Size: 2349 256 1366
Fragments arranged by size:
2349 1366 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3971
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3971
AvaI C'yCGr_G
Cuts at: 223 319 991 223
Size: 96 672 3203
Fragments arranged by size:
3203 672 96
BamHI G'GATC_C
Cuts at: 996 996
Size: 3971
BanI G'GyrC_C
Cuts at: 149 814 987 1401 1649 1684 149
Size: 665 173 414 248 35 2436
Fragments arranged by size:
2436 665 414 248 173 35
BanII G_rGCy'C
Cuts at: 985 2015 985
Size: 1030 2941
BbeI G_GCGC'C
Cuts at: 153 818 1405 1653 153
Size: 665 587 248 2471
Fragments arranged by size:
2471 665 587 248
BbsI GAAGACnn'nnnn_
Cuts at: 737 737
Size: 3971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 730 764 730
Size: 34 3937
BciVI GTATCCnnnnn_n'
Cuts at: 1869 2630 3971 1869
Size: 761 1341 1869
Fragments arranged by size:
1869 1341 761
BclI T'GATC_A
Cuts at: 1491 1491
Size: 3971
BglI GCCn_nnn'nGGC
Cuts at: 497 497
Size: 3971
BglII A'GATC_T
Cuts at: 479 1486 3523 479
Size: 1007 2037 927
Fragments arranged by size:
2037 1007 927
BlpI GC'TnA_GC
Cuts at: 1083 1083
Size: 3971
Bme1580I G_kGCm'C
Cuts at: 1596 1689 2745 1596
Size: 93 1056 2822
Fragments arranged by size:
2822 1056 93
BmgBI CAC'GTC
Cuts at: 612 612
Size: 3971
BmrI ACTGGGnnnn_n'
Cuts at: 1183 1264 1599 3743 1183
Size: 81 335 2144 1411
Fragments arranged by size:
2144 1411 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2338 2338
Size: 3971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 938 938
Size: 3971
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1147 2026 2518 2816 847
Size: 300 879 492 298 2002
Fragments arranged by size:
2002 879 492 300 298
BsaI GGTCTCn'nnnn_
Cuts at: 274 829 3675 274
Size: 555 2846 570
Fragments arranged by size:
2846 570 555
BsaAI yAC'GTr
Cuts at: 1324 1324
Size: 3971
BsaBI GATnn'nnATC
Cuts at: 6 1485 3522 6
Size: 1479 2037 455
Fragments arranged by size:
2037 1479 455
BsaHI Gr'CG_yC
Cuts at: 150 641 815 1402 1650 2352 3848 150
Size: 491 174 587 248 702 1496 273
Fragments arranged by size:
1496 702 587 491 273 248 174
BsaWI w'CCGG_w
Cuts at: 1160 1681 2359 2633 2780 3191 1160
Size: 521 678 274 147 411 1940
Fragments arranged by size:
1940 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 650 680 650
Size: 30 3941
BseYI C'CCAG_C
Cuts at: 620 1396 2154 2731 3227 620
Size: 776 758 577 496 1364
Fragments arranged by size:
1364 776 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 634
Size: 3971
BsiEI CG_ry'CG
Cuts at: 104 636 1513 1559 2767 104
Size: 532 877 46 1208 1308
Fragments arranged by size:
1308 1208 877 532 46
BsiHKAI G_wGCw'C
Cuts at: 717 850 985 1763 1953 2745 717
Size: 133 135 778 190 792 1943
Fragments arranged by size:
1943 792 778 190 135 133
BsmBI CGTCTCn'nnnn_
Cuts at: 393 648 393
Size: 255 3716
Bsp1286I G_dGCh'C
Cuts at: 717 850 985 1596 1689 1763 1953 2015 2745
Size: 133 135 611 93 74 190 62 730
Cuts at: 2745 717
Size: 1943
Fragments arranged by size:
1943 730 611 190 135 133 93 74
62
BspEI T'CCGG_A
Cuts at: 3191 3191
Size: 3971
BspHI T'CATG_A
Cuts at: 3147 3147
Size: 3971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 521 593 1021 1235 1537 1918 521
Size: 72 428 214 302 381 2574
Fragments arranged by size:
2574 428 381 302 214 72
BsrBI CCG'CTC
Cuts at: 50 2263 2317 3801 3966 50
Size: 2213 54 1484 165 55
Fragments arranged by size:
2213 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1883 1883
Size: 3971
BsrFI r'CCGG_y
Cuts at: 1969 2150 2359 1969
Size: 181 209 3581
Fragments arranged by size:
3581 209 181
BssSI C'ACGA_G
Cuts at: 349 2242 2600 349
Size: 1893 358 1720
Fragments arranged by size:
1893 1720 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1348 1348
Size: 3971
BstBI TT'CG_AA
Cuts at: 2332 2332
Size: 3971
BstYI r'GATC_y
Cuts at: 1 479 857 996 1463 1486 1821 2067 3079
Size: 478 378 139 467 23 335 246 1012
Cuts at: 3079 3165 3177 3523 1
Size: 86 12 346 449
Fragments arranged by size:
1012 478 467 449 378 346 335 246
139 86 23 12
BtgI C'CryG_G
Cuts at: 339 492 636 339
Size: 153 144 3674
Fragments arranged by size:
3674 153 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 441 1921 2102 2265 441
Size: 1480 181 163 2147
Fragments arranged by size:
2147 1480 181 163
BtsI GCAGTG_nn'
Cuts at: 1214 1214
Size: 3971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 774 809 774
Size: 35 3936
DraI TTT'AAA
Cuts at: 970 970
Size: 3971
DraIII CAC_nnn'GTG
Cuts at: 723 723
Size: 3971
DrdI GACnn_nn'nnGTC
Cuts at: 640 1677 2535 640
Size: 1037 858 2076
Fragments arranged by size:
2076 1037 858
EaeI y'GGCC_r
Cuts at: 174 461 671 1556 1730 2121 2148 3493 174
Size: 287 210 885 174 391 27 1345 652
Fragments arranged by size:
1345 885 652 391 287 210 174 27
EagI C'GGCC_G
Cuts at: 1556 1556
Size: 3971
EarI CTCTTCn'nnn_
Cuts at: 1994 2204 1994
Size: 210 3761
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 552 939 2179 2489 2635 3781 552
Size: 387 1240 310 146 1146 742
Fragments arranged by size:
1240 1146 742 387 310 146
EcoICRI GAG'CTC
Cuts at: 983 983
Size: 3971
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 938 1796 2228 2975 3243 938
Size: 858 432 747 268 1666
Fragments arranged by size:
1666 858 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 570 1110 570
Size: 540 3431
EcoRI G'AATT_C
Cuts at: 975 975
Size: 3971
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 291 711 1990 291
Size: 420 1279 2272
Fragments arranged by size:
2272 1279 420
FspI TGC'GCA
Cuts at: 1752 1752
Size: 3971
HaeII r_GCGC'y
Cuts at: 153 818 1405 1653 2675 3654 153
Size: 665 587 248 1022 979 470
Fragments arranged by size:
1022 979 665 587 470 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 502 534 1813 1843 1845 1875 502
Size: 32 1279 30 2 30 2598
Fragments arranged by size:
2598 1279 32 30 30 2
HincII GTy'rAC
Cuts at: 1010 1010
Size: 3971
KasI G'GCGC_C
Cuts at: 149 814 1401 1649 149
Size: 665 587 248 2471
Fragments arranged by size:
2471 665 587 248
KpnI G_GTAC'C
Cuts at: 991 991
Size: 3971
MluI A'CGCG_T
Cuts at: 3404 3404
Size: 3971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 137 2642 2826 137
Size: 2505 184 1282
Fragments arranged by size:
2505 1282 184
MscI TGG'CCA
Cuts at: 673 1732 673
Size: 1059 2912
MslI CAynn'nnrTG
Cuts at: 353 556 1960 2087 353
Size: 203 1404 127 2237
Fragments arranged by size:
2237 1404 203 127
MspA1I CmG'CkG
Cuts at: 762 1082 1396 1756 2769 3014 762
Size: 320 314 360 1013 245 1719
Fragments arranged by size:
1719 1013 360 320 314 245
NaeI GCC'GGC
Cuts at: 2152 2152
Size: 3971
NarI GG'CG_CC
Cuts at: 150 815 1402 1650 150
Size: 665 587 248 2471
Fragments arranged by size:
2471 665 587 248
NgoMIV G'CCGG_C
Cuts at: 2150 2150
Size: 3971
NspI r_CATG'y
Cuts at: 1024 1524 2431 1024
Size: 500 907 2564
Fragments arranged by size:
2564 907 500
PciI A'CATG_T
Cuts at: 2427 2427
Size: 3971
PmeI GTTT'AAAC
Cuts at: 970 970
Size: 3971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3110 3142 3110
Size: 32 3939
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 883 915 883
Size: 32 3939
PstI C_TGCA'G
Cuts at: 518 1018 1703 518
Size: 500 685 2786
Fragments arranged by size:
2786 685 500
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3971
PvuII CAG'CTG
Cuts at: 762 1396 1756 762
Size: 634 360 2977
Fragments arranged by size:
2977 634 360
RsrII CG'GwC_CG
Cuts at: 2166 2166
Size: 3971
SacI G_AGCT'C
Cuts at: 985 985
Size: 3971
SalI G'TCGA_C
Cuts at: 1008 1008
Size: 3971
SapI GCTCTTCn'nnn_
Cuts at: 1994 2204 1994
Size: 210 3761
SbfI CC_TGCA'GG
Cuts at: 1018 1018
Size: 3971
ScaI AGT'ACT
Cuts at: 181 3488 181
Size: 3307 664
SexAI A'CCwGG_T
Cuts at: 719 719
Size: 3971
SfcI C'TryA_G
Cuts at: 33 514 1014 1699 2692 2883 33
Size: 481 500 685 993 191 1121
Fragments arranged by size:
1121 993 685 500 481 191
SfoI GGC'GCC
Cuts at: 151 816 1403 1651 151
Size: 665 587 248 2471
Fragments arranged by size:
2471 665 587 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3971
SmaI CCC'GGG
Cuts at: 993 993
Size: 3971
SmlI C'TyrA_G
Cuts at: 862 1126 2041 2533 2795 862
Size: 264 915 492 262 2038
Fragments arranged by size:
2038 915 492 264 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3971
SphI G_CATG'C
Cuts at: 1024 1524 1024
Size: 500 3471
StuI AGG'CCT
Cuts at: 500 500
Size: 3971
StyI C'CwwG_G
Cuts at: 275 1105 1459 275
Size: 830 354 2787
Fragments arranged by size:
2787 830 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1554 1687 2146 2355 1554
Size: 133 459 209 3170
Fragments arranged by size:
3170 459 209 133
TatI w'GTAC_w
Cuts at: 179 3486 179
Size: 3307 664
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 650 682 3488 3520 650
Size: 32 2806 32 1101
Fragments arranged by size:
2806 1101 32 32
Tth111I GACn'n_nGTC
Cuts at: 1768 1768
Size: 3971
XbaI T'CTAG_A
Cuts at: 1002 1002
Size: 3971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 759 825 1466 759
Size: 66 641 3264
Fragments arranged by size:
3264 641 66
XmaI C'CCGG_G
Cuts at: 991 991
Size: 3971
XmnI GAAnn'nnTTC
Cuts at: 328 427 328
Size: 99 3872
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmtI Bpu10I BseRI BsiWI
BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI