Restriction map for pF1KB6418
 (Circular) (Six-base) MAPSORT of: pF1KB6418.seq  Check: 8374  from: 1  to: 4124



pF1KB6418  4124 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AccI GT'mk_AC

Cuts at:    428    1162     428 
   Size:        734    3390
 
Acc65I G'GTAC_C

Cuts at:   1140    1140 
   Size:       4124
 
AclI AA'CG_TT

Cuts at:   3964    3964 
   Size:       4124
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1949    2381    3128    3396    1949 
   Size:        432     747     268    2677

  Fragments arranged by size: 

                2677     747     432     268
 
AflIII A'CryG_T

Cuts at:   1476    2580    3557    1476 
   Size:       1104     977    2043

  Fragments arranged by size: 

                2043    1104     977
 
AgeI A'CCGG_T

Cuts at:   2512    2512 
   Size:       4124
 
AleI CACnn'nnGTG

Cuts at:    175     178     175 
   Size:          3    4121
 
AlwNI CAG_nnn'CTG

Cuts at:    288    1429    2996     288 
   Size:       1141    1567    1416

  Fragments arranged by size: 

                1567    1416    1141
 
ApaLI G'TGCA_C

Cuts at:   2894    2894 
   Size:       4124
 
ApoI r'AATT_y

Cuts at:    521     554    1128    3477    3733     521 
   Size:         33     574    2349     256     912

  Fragments arranged by size: 

                2349     912     574     256      33
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4124
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4124
 
AvaI C'yCGr_G

Cuts at:    574    1144     574 
   Size:        570    3554
 
BamHI G'GATC_C

Cuts at:   1040    1149    1040 
   Size:        109    4015
 
BanI G'GyrC_C

Cuts at:    811     918    1140    1554    1802    1837     811 
   Size:        107     222     414     248      35    3098

  Fragments arranged by size: 

                3098     414     248     222     107      35
 
BanII G_rGCy'C

Cuts at:    118     491     575     731    1138    2168     118 
   Size:        373      84     156     407    1030    2074

  Fragments arranged by size: 

                2074    1030     407     373     156      84
 
BbeI G_GCGC'C

Cuts at:    815    1558    1806     815 
   Size:        743     248    3133

  Fragments arranged by size: 

                3133     743     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    743     743 
   Size:       4124
 
BciVI GTATCCnnnnn_n'

Cuts at:    426    2022    2783    4124     426 
   Size:       1596     761    1341     426

  Fragments arranged by size: 

                1596    1341     761     426
 
BclI T'GATC_A

Cuts at:   1644    1644 
   Size:       4124
 
BglI GCCn_nnn'nGGC

Cuts at:    940     940 
   Size:       4124
 
BglII A'GATC_T

Cuts at:   1639    3676    1639 
   Size:       2037    2087
 
BlpI GC'TnA_GC

Cuts at:   1236    1236 
   Size:       4124
 
Bme1580I G_kGCm'C

Cuts at:   1749    1842    2898    1749 
   Size:         93    1056    2975

  Fragments arranged by size: 

                2975    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1336    1417    1752    3896    1336 
   Size:         81     335    2144    1564

  Fragments arranged by size: 

                2144    1564     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1047    2491    1047 
   Size:       1444    2680
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1093    1093 
   Size:       4124
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    145    1300    2179    2671    2969     145 
   Size:       1155     879     492     298    1300

  Fragments arranged by size: 

                1300    1155     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3828    3828 
   Size:       4124
 
BsaAI yAC'GTr

Cuts at:   1477    1477 
   Size:       4124
 
BsaBI GATnn'nnATC

Cuts at:      6    1638    3675       6 
   Size:       1632    2037     455

  Fragments arranged by size: 

                2037    1632     455
 
BsaHI Gr'CG_yC

Cuts at:    812    1555    1803    2505    4001     812 
   Size:        743     248     702    1496     935

  Fragments arranged by size: 

                1496     935     743     702     248
 
BsaWI w'CCGG_w

Cuts at:   1313    1834    2512    2786    2933    3344    1313 
   Size:        521     678     274     147     411    2093

  Fragments arranged by size: 

                2093     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    866     896     866 
   Size:         30    4094
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    310     592     310 
   Size:        282    3842
 
BseYI C'CCAG_C

Cuts at:    321     502    1549    2307    2884    3380     321 
   Size:        181    1047     758     577     496    1065

  Fragments arranged by size: 

                1065    1047     758     577     496     181
 
BsiEI CG_ry'CG

Cuts at:    104    1666    1712    2920     104 
   Size:       1562      46    1208    1308

  Fragments arranged by size: 

                1562    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    118     491     575     929    1138    1916    2106    2898     118 
   Size:        373      84     354     209     778     190     792    1344

  Fragments arranged by size: 

                1344     792     778     373     354     209     190      84
            
 
Bsp1286I G_dGCh'C

Cuts at:    118     491     575     731     929    1138    1749    1842    1916 
   Size:        373      84     156     198     209     611      93      74

Cuts at:   1916    2106    2168    2898     118 
   Size:        190      62     730    1344

  Fragments arranged by size: 

                1344     730     611     373     209     198     190     156
                  93      84      74      62
 
BspEI T'CCGG_A

Cuts at:   3344    3344 
   Size:       4124
 
BspHI T'CATG_A

Cuts at:   3300    3300 
   Size:       4124
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1174    1388    1690    2071    1174 
   Size:        214     302     381    3227

  Fragments arranged by size: 

                3227     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1077    2416    2470    3954    4119      50 
   Size:       1027    1339      54    1484     165      55

  Fragments arranged by size: 

                1484    1339    1027     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2036    2036 
   Size:       4124
 
BsrFI r'CCGG_y

Cuts at:    850    2122    2303    2512     850 
   Size:       1272     181     209    2462

  Fragments arranged by size: 

                2462    1272     209     181
 
BssSI C'ACGA_G

Cuts at:   2395    2753    2395 
   Size:        358    3766
 
BstAPI GCAn_nnn'nTGC

Cuts at:    596    1501     596 
   Size:        905    3219
 
BstBI TT'CG_AA

Cuts at:   2485    2485 
   Size:       4124
 
BstXI CCAn_nnnn'nTGG

Cuts at:    180     807     885     957     180 
   Size:        627      78      72    3347

  Fragments arranged by size: 

                3347     627      78      72
 
BstYI r'GATC_y

Cuts at:      1    1040    1149    1616    1639    1974    2220    3232    3318 
   Size:       1039     109     467      23     335     246    1012      86

Cuts at:   3318    3330    3676       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1039    1012     467     449     346     335     246     109
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    176     176 
   Size:       4124
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    472    2074    2255    2418     472 
   Size:       1602     181     163    2178

  Fragments arranged by size: 

                2178    1602     181     163
 
BtsI GCAGTG_nn'

Cuts at:    594    1070    1367     594 
   Size:        476     297    3351

  Fragments arranged by size: 

                3351     476     297
 
DraI TTT'AAA

Cuts at:   1123    1123 
   Size:       4124
 
DrdI GACnn_nn'nnGTC

Cuts at:   1830    2688    1830 
   Size:        858    3266
 
EaeI y'GGCC_r

Cuts at:    182     257     312     464     848    1709    1883    2274    2301 
   Size:         75      55     152     384     861     174     391      27

Cuts at:   2301    3646     182 
   Size:       1345     660

  Fragments arranged by size: 

                1345     861     660     391     384     174     152      75
                  55      27
 
EagI C'GGCC_G

Cuts at:   1709    1709 
   Size:       4124
 
EarI CTCTTCn'nnn_

Cuts at:    619    2147    2357     619 
   Size:       1528     210    2386

  Fragments arranged by size: 

                2386    1528     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2332    2642    2788    3934    2332 
   Size:        310     146    1146    2522

  Fragments arranged by size: 

                2522    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    116     489     573    1136     116 
   Size:        373      84     563    3104

  Fragments arranged by size: 

                3104     563     373      84
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1093    1949    2381    3128    3396    1093 
   Size:        856     432     747     268    1821

  Fragments arranged by size: 

                1821     856     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:   1263    1263 
   Size:       4124
 
EcoRI G'AATT_C

Cuts at:    521     554    1128     521 
   Size:         33     574    3517

  Fragments arranged by size: 

                3517     574      33
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2143    2143 
   Size:       4124
 
FspI TGC'GCA

Cuts at:   1905    1905 
   Size:       4124
 
HaeII r_GCGC'y

Cuts at:    815    1558    1806    2828    3807     815 
   Size:        743     248    1022     979    1132

  Fragments arranged by size: 

                1132    1022     979     743     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    703     735     967     999    1966    1996    1998    2028     703 
   Size:         32     232      32     967      30       2      30    2799

  Fragments arranged by size: 

                2799     967     232      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1163    1163 
   Size:       4124
 
KasI G'GCGC_C

Cuts at:    811    1554    1802     811 
   Size:        743     248    3133

  Fragments arranged by size: 

                3133     743     248
 
KpnI G_GTAC'C

Cuts at:   1144    1144 
   Size:       4124
 
MluI A'CGCG_T

Cuts at:   3557    3557 
   Size:       4124
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2795    2979    2795 
   Size:        184    3940
 
MscI TGG'CCA

Cuts at:    466    1885     466 
   Size:       1419    2705
 
MslI CAynn'nnrTG

Cuts at:    175     178     805     974    2113    2240     175 
   Size:          3     627     169    1139     127    2059

  Fragments arranged by size: 

                2059    1139     627     169     127       3
 
MspA1I CmG'CkG

Cuts at:   1057    1235    1549    1909    2922    3167    1057 
   Size:        178     314     360    1013     245    2014

  Fragments arranged by size: 

                2014    1013     360     314     245     178
 
NaeI GCC'GGC

Cuts at:    852    2305     852 
   Size:       1453    2671
 
NarI GG'CG_CC

Cuts at:    812    1555    1803     812 
   Size:        743     248    3133

  Fragments arranged by size: 

                3133     743     248
 
NgoMIV G'CCGG_C

Cuts at:    850    2303     850 
   Size:       1453    2671
 
NspI r_CATG'y

Cuts at:    700    1177    1677    2584     700 
   Size:        477     500     907    2240

  Fragments arranged by size: 

                2240     907     500     477
 
PasI CC'CwG_GG

Cuts at:    742     742 
   Size:       4124
 
PciI A'CATG_T

Cuts at:   2580    2580 
   Size:       4124
 
PflMI CCAn_nnn'nTGG

Cuts at:    288     633     676     288 
   Size:        345      43    3736

  Fragments arranged by size: 

                3736     345      43
 
PmeI GTTT'AAAC

Cuts at:   1123    1123 
   Size:       4124
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3263    3295    3263 
   Size:         32    4092
 
PsiI TTA'TAA

Cuts at:    529     529 
   Size:       4124
 
PspXI vC'TCGA_Gb

Cuts at:    574     574 
   Size:       4124
 
PstI C_TGCA'G

Cuts at:    902    1171    1856     902 
   Size:        269     685    3170

  Fragments arranged by size: 

                3170     685     269
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4124
 
PvuII CAG'CTG

Cuts at:   1549    1909    1549 
   Size:        360    3764
 
RsrII CG'GwC_CG

Cuts at:   2319    2319 
   Size:       4124
 
SacI G_AGCT'C

Cuts at:    118     491     575    1138     118 
   Size:        373      84     563    3104

  Fragments arranged by size: 

                3104     563     373      84
 
SalI G'TCGA_C

Cuts at:   1161    1161 
   Size:       4124
 
SapI GCTCTTCn'nnn_

Cuts at:   2147    2357    2147 
   Size:        210    3914
 
SbfI CC_TGCA'GG

Cuts at:   1171    1171 
   Size:       4124
 
ScaI AGT'ACT

Cuts at:   3641    3641 
   Size:       4124
 
SfcI C'TryA_G

Cuts at:     33     898    1167    1852    2845    3036      33 
   Size:        865     269     685     993     191    1121

  Fragments arranged by size: 

                1121     993     865     685     269     191
 
SfoI GGC'GCC

Cuts at:    813    1556    1804     813 
   Size:        743     248    3133

  Fragments arranged by size: 

                3133     743     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4124
 
SmaI CCC'GGG

Cuts at:   1146    1146 
   Size:       4124
 
SmlI C'TyrA_G

Cuts at:    160     574    1279    2194    2686    2948     160 
   Size:        414     705     915     492     262    1336

  Fragments arranged by size: 

                1336     915     705     492     414     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4124
 
SphI G_CATG'C

Cuts at:   1177    1677    1177 
   Size:        500    3624
 
StyI C'CwwG_G

Cuts at:    935    1258    1612     935 
   Size:        323     354    3447

  Fragments arranged by size: 

                3447     354     323
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    533     891    1707    1840    2299    2508     533 
   Size:        358     816     133     459     209    2149

  Fragments arranged by size: 

                2149     816     459     358     209     133
 
TatI w'GTAC_w

Cuts at:    215    3639     215 
   Size:       3424     700
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    866     898    3641    3673     866 
   Size:         32    2743      32    1317

  Fragments arranged by size: 

                2743    1317      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1921    1921 
   Size:       4124
 
XbaI T'CTAG_A

Cuts at:   1155    1155 
   Size:       4124
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1619    1619 
   Size:       4124
 
XhoI C'TCGA_G

Cuts at:    574     574 
   Size:       4124
 
XmaI C'CCGG_G

Cuts at:   1144    1144 
   Size:       4124
 
XmnI GAAnn'nnTTC

Cuts at:    554     985     554 
   Size:        431    3693
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI
     PciI    PflMI     PmeI     PpiI     PsiI    PspXI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI
   Bpu10I     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII
  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PfoI     PmlI    PpuMI    PshAI   PspOMI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     ZraI
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