(Circular) (Six-base) MAPSORT of: pF1KB6418.seq Check: 8374 from: 1 to: 4124 pF1KB6418 4124 bp With 182 enzymes: SgfI * April 24, 2009 13:05 .. AccI GT'mk_AC Cuts at: 428 1162 428 Size: 734 3390 Acc65I G'GTAC_C Cuts at: 1140 1140 Size: 4124 AclI AA'CG_TT Cuts at: 3964 3964 Size: 4124 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1949 2381 3128 3396 1949 Size: 432 747 268 2677 Fragments arranged by size: 2677 747 432 268 AflIII A'CryG_T Cuts at: 1476 2580 3557 1476 Size: 1104 977 2043 Fragments arranged by size: 2043 1104 977 AgeI A'CCGG_T Cuts at: 2512 2512 Size: 4124 AleI CACnn'nnGTG Cuts at: 175 178 175 Size: 3 4121 AlwNI CAG_nnn'CTG Cuts at: 288 1429 2996 288 Size: 1141 1567 1416 Fragments arranged by size: 1567 1416 1141 ApaLI G'TGCA_C Cuts at: 2894 2894 Size: 4124 ApoI r'AATT_y Cuts at: 521 554 1128 3477 3733 521 Size: 33 574 2349 256 912 Fragments arranged by size: 2349 912 574 256 33 AseI AT'TA_AT Cuts at: 20 20 Size: 4124 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4124 AvaI C'yCGr_G Cuts at: 574 1144 574 Size: 570 3554 BamHI G'GATC_C Cuts at: 1040 1149 1040 Size: 109 4015 BanI G'GyrC_C Cuts at: 811 918 1140 1554 1802 1837 811 Size: 107 222 414 248 35 3098 Fragments arranged by size: 3098 414 248 222 107 35 BanII G_rGCy'C Cuts at: 118 491 575 731 1138 2168 118 Size: 373 84 156 407 1030 2074 Fragments arranged by size: 2074 1030 407 373 156 84 BbeI G_GCGC'C Cuts at: 815 1558 1806 815 Size: 743 248 3133 Fragments arranged by size: 3133 743 248 BbsI GAAGACnn'nnnn_ Cuts at: 743 743 Size: 4124 BciVI GTATCCnnnnn_n' Cuts at: 426 2022 2783 4124 426 Size: 1596 761 1341 426 Fragments arranged by size: 1596 1341 761 426 BclI T'GATC_A Cuts at: 1644 1644 Size: 4124 BglI GCCn_nnn'nGGC Cuts at: 940 940 Size: 4124 BglII A'GATC_T Cuts at: 1639 3676 1639 Size: 2037 2087 BlpI GC'TnA_GC Cuts at: 1236 1236 Size: 4124 Bme1580I G_kGCm'C Cuts at: 1749 1842 2898 1749 Size: 93 1056 2975 Fragments arranged by size: 2975 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1336 1417 1752 3896 1336 Size: 81 335 2144 1564 Fragments arranged by size: 2144 1564 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1047 2491 1047 Size: 1444 2680 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1093 1093 Size: 4124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 145 1300 2179 2671 2969 145 Size: 1155 879 492 298 1300 Fragments arranged by size: 1300 1155 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3828 3828 Size: 4124 BsaAI yAC'GTr Cuts at: 1477 1477 Size: 4124 BsaBI GATnn'nnATC Cuts at: 6 1638 3675 6 Size: 1632 2037 455 Fragments arranged by size: 2037 1632 455 BsaHI Gr'CG_yC Cuts at: 812 1555 1803 2505 4001 812 Size: 743 248 702 1496 935 Fragments arranged by size: 1496 935 743 702 248 BsaWI w'CCGG_w Cuts at: 1313 1834 2512 2786 2933 3344 1313 Size: 521 678 274 147 411 2093 Fragments arranged by size: 2093 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 866 896 866 Size: 30 4094 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 310 592 310 Size: 282 3842 BseYI C'CCAG_C Cuts at: 321 502 1549 2307 2884 3380 321 Size: 181 1047 758 577 496 1065 Fragments arranged by size: 1065 1047 758 577 496 181 BsiEI CG_ry'CG Cuts at: 104 1666 1712 2920 104 Size: 1562 46 1208 1308 Fragments arranged by size: 1562 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 118 491 575 929 1138 1916 2106 2898 118 Size: 373 84 354 209 778 190 792 1344 Fragments arranged by size: 1344 792 778 373 354 209 190 84 Bsp1286I G_dGCh'C Cuts at: 118 491 575 731 929 1138 1749 1842 1916 Size: 373 84 156 198 209 611 93 74 Cuts at: 1916 2106 2168 2898 118 Size: 190 62 730 1344 Fragments arranged by size: 1344 730 611 373 209 198 190 156 93 84 74 62 BspEI T'CCGG_A Cuts at: 3344 3344 Size: 4124 BspHI T'CATG_A Cuts at: 3300 3300 Size: 4124 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1174 1388 1690 2071 1174 Size: 214 302 381 3227 Fragments arranged by size: 3227 381 302 214 BsrBI CCG'CTC Cuts at: 50 1077 2416 2470 3954 4119 50 Size: 1027 1339 54 1484 165 55 Fragments arranged by size: 1484 1339 1027 165 55 54 BsrDI GCAATG_nn' Cuts at: 2036 2036 Size: 4124 BsrFI r'CCGG_y Cuts at: 850 2122 2303 2512 850 Size: 1272 181 209 2462 Fragments arranged by size: 2462 1272 209 181 BssSI C'ACGA_G Cuts at: 2395 2753 2395 Size: 358 3766 BstAPI GCAn_nnn'nTGC Cuts at: 596 1501 596 Size: 905 3219 BstBI TT'CG_AA Cuts at: 2485 2485 Size: 4124 BstXI CCAn_nnnn'nTGG Cuts at: 180 807 885 957 180 Size: 627 78 72 3347 Fragments arranged by size: 3347 627 78 72 BstYI r'GATC_y Cuts at: 1 1040 1149 1616 1639 1974 2220 3232 3318 Size: 1039 109 467 23 335 246 1012 86 Cuts at: 3318 3330 3676 1 Size: 12 346 449 Fragments arranged by size: 1039 1012 467 449 346 335 246 109 86 23 12 BtgI C'CryG_G Cuts at: 176 176 Size: 4124 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 472 2074 2255 2418 472 Size: 1602 181 163 2178 Fragments arranged by size: 2178 1602 181 163 BtsI GCAGTG_nn' Cuts at: 594 1070 1367 594 Size: 476 297 3351 Fragments arranged by size: 3351 476 297 DraI TTT'AAA Cuts at: 1123 1123 Size: 4124 DrdI GACnn_nn'nnGTC Cuts at: 1830 2688 1830 Size: 858 3266 EaeI y'GGCC_r Cuts at: 182 257 312 464 848 1709 1883 2274 2301 Size: 75 55 152 384 861 174 391 27 Cuts at: 2301 3646 182 Size: 1345 660 Fragments arranged by size: 1345 861 660 391 384 174 152 75 55 27 EagI C'GGCC_G Cuts at: 1709 1709 Size: 4124 EarI CTCTTCn'nnn_ Cuts at: 619 2147 2357 619 Size: 1528 210 2386 Fragments arranged by size: 2386 1528 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2332 2642 2788 3934 2332 Size: 310 146 1146 2522 Fragments arranged by size: 2522 1146 310 146 EcoICRI GAG'CTC Cuts at: 116 489 573 1136 116 Size: 373 84 563 3104 Fragments arranged by size: 3104 563 373 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1093 1949 2381 3128 3396 1093 Size: 856 432 747 268 1821 Fragments arranged by size: 1821 856 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 1263 1263 Size: 4124 EcoRI G'AATT_C Cuts at: 521 554 1128 521 Size: 33 574 3517 Fragments arranged by size: 3517 574 33 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2143 2143 Size: 4124 FspI TGC'GCA Cuts at: 1905 1905 Size: 4124 HaeII r_GCGC'y Cuts at: 815 1558 1806 2828 3807 815 Size: 743 248 1022 979 1132 Fragments arranged by size: 1132 1022 979 743 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 703 735 967 999 1966 1996 1998 2028 703 Size: 32 232 32 967 30 2 30 2799 Fragments arranged by size: 2799 967 232 32 32 30 30 2 HincII GTy'rAC Cuts at: 1163 1163 Size: 4124 KasI G'GCGC_C Cuts at: 811 1554 1802 811 Size: 743 248 3133 Fragments arranged by size: 3133 743 248 KpnI G_GTAC'C Cuts at: 1144 1144 Size: 4124 MluI A'CGCG_T Cuts at: 3557 3557 Size: 4124 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2795 2979 2795 Size: 184 3940 MscI TGG'CCA Cuts at: 466 1885 466 Size: 1419 2705 MslI CAynn'nnrTG Cuts at: 175 178 805 974 2113 2240 175 Size: 3 627 169 1139 127 2059 Fragments arranged by size: 2059 1139 627 169 127 3 MspA1I CmG'CkG Cuts at: 1057 1235 1549 1909 2922 3167 1057 Size: 178 314 360 1013 245 2014 Fragments arranged by size: 2014 1013 360 314 245 178 NaeI GCC'GGC Cuts at: 852 2305 852 Size: 1453 2671 NarI GG'CG_CC Cuts at: 812 1555 1803 812 Size: 743 248 3133 Fragments arranged by size: 3133 743 248 NgoMIV G'CCGG_C Cuts at: 850 2303 850 Size: 1453 2671 NspI r_CATG'y Cuts at: 700 1177 1677 2584 700 Size: 477 500 907 2240 Fragments arranged by size: 2240 907 500 477 PasI CC'CwG_GG Cuts at: 742 742 Size: 4124 PciI A'CATG_T Cuts at: 2580 2580 Size: 4124 PflMI CCAn_nnn'nTGG Cuts at: 288 633 676 288 Size: 345 43 3736 Fragments arranged by size: 3736 345 43 PmeI GTTT'AAAC Cuts at: 1123 1123 Size: 4124 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3263 3295 3263 Size: 32 4092 PsiI TTA'TAA Cuts at: 529 529 Size: 4124 PspXI vC'TCGA_Gb Cuts at: 574 574 Size: 4124 PstI C_TGCA'G Cuts at: 902 1171 1856 902 Size: 269 685 3170 Fragments arranged by size: 3170 685 269 PvuI CG_AT'CG Cuts at: 104 104 Size: 4124 PvuII CAG'CTG Cuts at: 1549 1909 1549 Size: 360 3764 RsrII CG'GwC_CG Cuts at: 2319 2319 Size: 4124 SacI G_AGCT'C Cuts at: 118 491 575 1138 118 Size: 373 84 563 3104 Fragments arranged by size: 3104 563 373 84 SalI G'TCGA_C Cuts at: 1161 1161 Size: 4124 SapI GCTCTTCn'nnn_ Cuts at: 2147 2357 2147 Size: 210 3914 SbfI CC_TGCA'GG Cuts at: 1171 1171 Size: 4124 ScaI AGT'ACT Cuts at: 3641 3641 Size: 4124 SfcI C'TryA_G Cuts at: 33 898 1167 1852 2845 3036 33 Size: 865 269 685 993 191 1121 Fragments arranged by size: 1121 993 865 685 269 191 SfoI GGC'GCC Cuts at: 813 1556 1804 813 Size: 743 248 3133 Fragments arranged by size: 3133 743 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4124 SmaI CCC'GGG Cuts at: 1146 1146 Size: 4124 SmlI C'TyrA_G Cuts at: 160 574 1279 2194 2686 2948 160 Size: 414 705 915 492 262 1336 Fragments arranged by size: 1336 915 705 492 414 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4124 SphI G_CATG'C Cuts at: 1177 1677 1177 Size: 500 3624 StyI C'CwwG_G Cuts at: 935 1258 1612 935 Size: 323 354 3447 Fragments arranged by size: 3447 354 323 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 533 891 1707 1840 2299 2508 533 Size: 358 816 133 459 209 2149 Fragments arranged by size: 2149 816 459 358 209 133 TatI w'GTAC_w Cuts at: 215 3639 215 Size: 3424 700 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 866 898 3641 3673 866 Size: 32 2743 32 1317 Fragments arranged by size: 2743 1317 32 32 Tth111I GACn'n_nGTC Cuts at: 1921 1921 Size: 4124 XbaI T'CTAG_A Cuts at: 1155 1155 Size: 4124 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1619 1619 Size: 4124 XhoI C'TCGA_G Cuts at: 574 574 Size: 4124 XmaI C'CCGG_G Cuts at: 1144 1144 Size: 4124 XmnI GAAnn'nnTTC Cuts at: 554 985 554 Size: 431 3693 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI PmeI PpiI PsiI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PfoI PmlI PpuMI PshAI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI ZraI