Restriction map for
pF1KB6414
(Circular) (Six-base) MAPSORT of: pF1KB6414.seq Check: 625 from: 1 to: 4118
pF1KB6414 4118 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 1156 1156
Size: 4118
Acc65I G'GTAC_C
Cuts at: 1134 1134
Size: 4118
AclI AA'CG_TT
Cuts at: 3958 3958
Size: 4118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 350 794 1943 2375 3122 3390 338
Size: 12 444 1149 432 747 268 1066
Fragments arranged by size:
1149 1066 747 444 432 268 12
AfeI AGC'GCT
Cuts at: 480 480
Size: 4118
AflIII A'CryG_T
Cuts at: 1470 2574 3551 1470
Size: 1104 977 2037
Fragments arranged by size:
2037 1104 977
AgeI A'CCGG_T
Cuts at: 2506 2506
Size: 4118
AlwNI CAG_nnn'CTG
Cuts at: 1423 2990 1423
Size: 1567 2551
ApaLI G'TGCA_C
Cuts at: 245 413 2888 245
Size: 168 2475 1475
Fragments arranged by size:
2475 1475 168
ApoI r'AATT_y
Cuts at: 384 608 905 1122 3471 3727 384
Size: 224 297 217 2349 256 775
Fragments arranged by size:
2349 775 297 256 224 217
AseI AT'TA_AT
Cuts at: 20 20
Size: 4118
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
AvaI C'yCGr_G
Cuts at: 1138 1138
Size: 4118
BamHI G'GATC_C
Cuts at: 1143 1143
Size: 4118
BanI G'GyrC_C
Cuts at: 225 1134 1548 1796 1831 225
Size: 909 414 248 35 2512
Fragments arranged by size:
2512 909 414 248 35
BanII G_rGCy'C
Cuts at: 237 734 1132 2162 237
Size: 497 398 1030 2193
Fragments arranged by size:
2193 1030 497 398
BbeI G_GCGC'C
Cuts at: 1552 1800 1552
Size: 248 3870
BbvCI CC'TCA_GC
Cuts at: 125 710 1016 125
Size: 585 306 3227
Fragments arranged by size:
3227 585 306
BciVI GTATCCnnnnn_n'
Cuts at: 2016 2777 4118 2016
Size: 761 1341 2016
Fragments arranged by size:
2016 1341 761
BclI T'GATC_A
Cuts at: 1638 1638
Size: 4118
BfrBI ATG'CAT
Cuts at: 454 454
Size: 4118
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4118
BglII A'GATC_T
Cuts at: 501 1633 3670 501
Size: 1132 2037 949
Fragments arranged by size:
2037 1132 949
BlpI GC'TnA_GC
Cuts at: 159 1230 159
Size: 1071 3047
Bme1580I G_kGCm'C
Cuts at: 228 249 417 1743 1836 2892 228
Size: 21 168 1326 93 1056 1454
Fragments arranged by size:
1454 1326 1056 168 93 21
BmrI ACTGGGnnnn_n'
Cuts at: 1330 1411 1746 3890 1330
Size: 81 335 2144 1558
Fragments arranged by size:
2144 1558 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 174 2485 174
Size: 2311 1807
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 746 746
Size: 4118
Bpu10I CC'TnA_GC
Cuts at: 125 710 1016 125
Size: 585 306 3227
Fragments arranged by size:
3227 585 306
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 554 1294 2173 2665 2963 275
Size: 279 740 879 492 298 1430
Fragments arranged by size:
1430 879 740 492 298 279
BsaI GGTCTCn'nnnn_
Cuts at: 3822 3822
Size: 4118
BsaAI yAC'GTr
Cuts at: 1471 1471
Size: 4118
BsaBI GATnn'nnATC
Cuts at: 6 1632 3669 6
Size: 1626 2037 455
Fragments arranged by size:
2037 1626 455
BsaHI Gr'CG_yC
Cuts at: 1549 1797 2499 3995 1549
Size: 248 702 1496 1672
Fragments arranged by size:
1672 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1307 1828 2506 2780 2927 3338 1307
Size: 521 678 274 147 411 2087
Fragments arranged by size:
2087 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1097 1127 1097
Size: 30 4088
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 190 557 1118 163
Size: 27 367 561 3163
Fragments arranged by size:
3163 561 367 27
BseYI C'CCAG_C
Cuts at: 475 1543 2301 2878 3374 475
Size: 1068 758 577 496 1219
Fragments arranged by size:
1219 1068 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 143 402 1660 1706 2914 104
Size: 39 259 1258 46 1208 1308
Fragments arranged by size:
1308 1258 1208 259 46 39
BsiHKAI G_wGCw'C
Cuts at: 249 417 734 1132 1910 2100 2892 249
Size: 168 317 398 778 190 792 1475
Fragments arranged by size:
1475 792 778 398 317 190 168
Bsp1286I G_dGCh'C
Cuts at: 228 237 249 417 734 1132 1743 1836 1910
Size: 9 12 168 317 398 611 93 74
Cuts at: 1910 2100 2162 2892 228
Size: 190 62 730 1454
Fragments arranged by size:
1454 730 611 398 317 190 168 93
74 62 12 9
BspEI T'CCGG_A
Cuts at: 3338 3338
Size: 4118
BspHI T'CATG_A
Cuts at: 3294 3294
Size: 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1168 1382 1684 2065 1168
Size: 214 302 381 3221
Fragments arranged by size:
3221 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2410 2464 3948 4113 50
Size: 2360 54 1484 165 55
Fragments arranged by size:
2360 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2030 2030
Size: 4118
BsrFI r'CCGG_y
Cuts at: 2116 2297 2506 2116
Size: 181 209 3728
Fragments arranged by size:
3728 209 181
BssSI C'ACGA_G
Cuts at: 2389 2747 2389
Size: 358 3760
BstAPI GCAn_nnn'nTGC
Cuts at: 1495 1495
Size: 4118
BstBI TT'CG_AA
Cuts at: 2479 2479
Size: 4118
BstYI r'GATC_y
Cuts at: 1 501 1143 1610 1633 1968 2214 3226 3312
Size: 500 642 467 23 335 246 1012 86
Cuts at: 3312 3324 3670 1
Size: 12 346 449
Fragments arranged by size:
1012 642 500 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 143 229 107
Size: 36 86 3996
Fragments arranged by size:
3996 86 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2068 2249 2412 2068
Size: 181 163 3774
Fragments arranged by size:
3774 181 163
BtsI GCAGTG_nn'
Cuts at: 1361 1361
Size: 4118
DraI TTT'AAA
Cuts at: 1117 1117
Size: 4118
DraIII CAC_nnn'GTG
Cuts at: 244 583 244
Size: 339 3779
DrdI GACnn_nn'nnGTC
Cuts at: 1824 2682 1824
Size: 858 3260
EaeI y'GGCC_r
Cuts at: 140 1003 1703 1877 2268 2295 3640 140
Size: 863 700 174 391 27 1345 618
Fragments arranged by size:
1345 863 700 618 391 174 27
EagI C'GGCC_G
Cuts at: 140 1703 140
Size: 1563 2555
EarI CTCTTCn'nnn_
Cuts at: 656 2141 2351 656
Size: 1485 210 2423
Fragments arranged by size:
2423 1485 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2326 2636 2782 3928 2326
Size: 310 146 1146 2516
Fragments arranged by size:
2516 1146 310 146
EcoICRI GAG'CTC
Cuts at: 732 1130 732
Size: 398 3720
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 350 746 794 1943 2375 3122 3390 338
Size: 12 396 48 1149 432 747 268 1066
Fragments arranged by size:
1149 1066 747 432 396 268 48 12
EcoO109I rG'GnC_Cy
Cuts at: 1257 1257
Size: 4118
EcoRI G'AATT_C
Cuts at: 384 1122 384
Size: 738 3380
EcoRV GAT'ATC
Cuts at: 648 648
Size: 4118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 4118
FspI TGC'GCA
Cuts at: 1899 1899
Size: 4118
HaeII r_GCGC'y
Cuts at: 482 1552 1800 2822 3801 482
Size: 1070 248 1022 979 799
Fragments arranged by size:
1070 1022 979 799 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 637 662 669 694 1960 1990 1992 2022 637
Size: 25 7 25 1266 30 2 30 2733
Fragments arranged by size:
2733 1266 30 30 25 25 7 2
HincII GTy'rAC
Cuts at: 431 1157 431
Size: 726 3392
HindIII A'AGCT_T
Cuts at: 842 989 842
Size: 147 3971
KasI G'GCGC_C
Cuts at: 1548 1796 1548
Size: 248 3870
KpnI G_GTAC'C
Cuts at: 1138 1138
Size: 4118
MluI A'CGCG_T
Cuts at: 3551 3551
Size: 4118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 290 2789 2973 290
Size: 2499 184 1435
Fragments arranged by size:
2499 1435 184
MscI TGG'CCA
Cuts at: 1005 1879 1005
Size: 874 3244
MslI CAynn'nnrTG
Cuts at: 2107 2234 2107
Size: 127 3991
MspA1I CmG'CkG
Cuts at: 133 1020 1229 1543 1903 2916 3161 133
Size: 887 209 314 360 1013 245 1090
Fragments arranged by size:
1090 1013 887 360 314 245 209
NaeI GCC'GGC
Cuts at: 2299 2299
Size: 4118
NarI GG'CG_CC
Cuts at: 1549 1797 1549
Size: 248 3870
NcoI C'CATG_G
Cuts at: 107 229 107
Size: 122 3996
NgoMIV G'CCGG_C
Cuts at: 2297 2297
Size: 4118
NsiI A_TGCA'T
Cuts at: 456 456
Size: 4118
NspI r_CATG'y
Cuts at: 421 454 549 1171 1671 2578 421
Size: 33 95 622 500 907 1961
Fragments arranged by size:
1961 907 622 500 95 33
PciI A'CATG_T
Cuts at: 2574 2574
Size: 4118
PflMI CCAn_nnn'nTGG
Cuts at: 583 583
Size: 4118
PmeI GTTT'AAAC
Cuts at: 1117 1117
Size: 4118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3257 3289 3257
Size: 32 4086
PstI C_TGCA'G
Cuts at: 1165 1850 1165
Size: 685 3433
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4118
PvuII CAG'CTG
Cuts at: 133 1020 1543 1903 133
Size: 887 523 360 2348
Fragments arranged by size:
2348 887 523 360
RsrII CG'GwC_CG
Cuts at: 2313 2313
Size: 4118
SacI G_AGCT'C
Cuts at: 734 1132 734
Size: 398 3720
SalI G'TCGA_C
Cuts at: 1155 1155
Size: 4118
SapI GCTCTTCn'nnn_
Cuts at: 2141 2351 2141
Size: 210 3908
SbfI CC_TGCA'GG
Cuts at: 1165 1165
Size: 4118
ScaI AGT'ACT
Cuts at: 927 3635 927
Size: 2708 1410
SfcI C'TryA_G
Cuts at: 33 134 1161 1846 2839 3030 33
Size: 101 1027 685 993 191 1121
Fragments arranged by size:
1121 1027 993 685 191 101
SfoI GGC'GCC
Cuts at: 1550 1798 1550
Size: 248 3870
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
SmaI CCC'GGG
Cuts at: 1140 1140
Size: 4118
SmlI C'TyrA_G
Cuts at: 290 569 1273 2188 2680 2942 290
Size: 279 704 915 492 262 1466
Fragments arranged by size:
1466 915 704 492 279 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4118
SphI G_CATG'C
Cuts at: 549 1171 1671 549
Size: 622 500 2996
Fragments arranged by size:
2996 622 500
SspI AAT'ATT
Cuts at: 627 627
Size: 4118
StyI C'CwwG_G
Cuts at: 107 229 1252 1606 107
Size: 122 1023 354 2619
Fragments arranged by size:
2619 1023 354 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 416 1701 1834 2293 2502 416
Size: 1285 133 459 209 2032
Fragments arranged by size:
2032 1285 459 209 133
TatI w'GTAC_w
Cuts at: 925 3633 925
Size: 2708 1410
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4086
Tth111I GACn'n_nGTC
Cuts at: 1915 1915
Size: 4118
XbaI T'CTAG_A
Cuts at: 1149 1149
Size: 4118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1613 1613
Size: 4118
XmaI C'CCGG_G
Cuts at: 1138 1138
Size: 4118
XmnI GAAnn'nnTTC
Cuts at: 323 611 665 971 323
Size: 288 54 306 3470
Fragments arranged by size:
3470 306 288 54
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BmgBI BmtI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoNI FseI FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI