Restriction map for
pF1KB6412
(Circular) (Six-base) MAPSORT of: pF1KB6412.seq Check: 2947 from: 1 to: 4118
pF1KB6412 4118 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 160 160
Size: 4118
AccI GT'mk_AC
Cuts at: 1156 1156
Size: 4118
Acc65I G'GTAC_C
Cuts at: 1134 1134
Size: 4118
AclI AA'CG_TT
Cuts at: 3958 3958
Size: 4118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 548 815 924 1943 2375 3122 3390 548
Size: 267 109 1019 432 747 268 1276
Fragments arranged by size:
1276 1019 747 432 268 267 109
AflIII A'CryG_T
Cuts at: 1470 2574 3551 1470
Size: 1104 977 2037
Fragments arranged by size:
2037 1104 977
AgeI A'CCGG_T
Cuts at: 2506 2506
Size: 4118
AleI CACnn'nnGTG
Cuts at: 256 605 929 256
Size: 349 324 3445
Fragments arranged by size:
3445 349 324
AlwNI CAG_nnn'CTG
Cuts at: 510 540 1423 2990 510
Size: 30 883 1567 1638
Fragments arranged by size:
1638 1567 883 30
ApaI G_GGCC'C
Cuts at: 712 712
Size: 4118
ApaLI G'TGCA_C
Cuts at: 2888 2888
Size: 4118
ApoI r'AATT_y
Cuts at: 1122 3471 3727 1122
Size: 2349 256 1513
Fragments arranged by size:
2349 1513 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4118
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
AvaI C'yCGr_G
Cuts at: 633 1138 633
Size: 505 3613
BamHI G'GATC_C
Cuts at: 560 1143 560
Size: 583 3535
BanI G'GyrC_C
Cuts at: 500 1134 1548 1796 1831 500
Size: 634 414 248 35 2787
Fragments arranged by size:
2787 634 414 248 35
BanII G_rGCy'C
Cuts at: 640 712 1132 2162 640
Size: 72 420 1030 2596
Fragments arranged by size:
2596 1030 420 72
BbeI G_GCGC'C
Cuts at: 1552 1800 1552
Size: 248 3870
BbsI GAAGACnn'nnnn_
Cuts at: 328 328
Size: 4118
BbvCI CC'TCA_GC
Cuts at: 1031 1031
Size: 4118
BciVI GTATCCnnnnn_n'
Cuts at: 2016 2777 4118 2016
Size: 761 1341 2016
Fragments arranged by size:
2016 1341 761
BclI T'GATC_A
Cuts at: 1638 1638
Size: 4118
BfrBI ATG'CAT
Cuts at: 458 458
Size: 4118
BglII A'GATC_T
Cuts at: 1633 3670 1633
Size: 2037 2081
BlpI GC'TnA_GC
Cuts at: 1230 1230
Size: 4118
Bme1580I G_kGCm'C
Cuts at: 712 1743 1836 2892 712
Size: 1031 93 1056 1938
Fragments arranged by size:
1938 1056 1031 93
BmrI ACTGGGnnnn_n'
Cuts at: 1330 1411 1746 3890 1330
Size: 81 335 2144 1558
Fragments arranged by size:
2144 1558 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1117 2485 1117
Size: 1368 2750
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 739 307
Size: 432 3686
Bpu10I CC'TnA_GC
Cuts at: 337 1031 337
Size: 694 3424
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1294 2173 2665 2963 1294
Size: 879 492 298 2449
Fragments arranged by size:
2449 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3822 3822
Size: 4118
BsaAI yAC'GTr
Cuts at: 1471 1471
Size: 4118
BsaBI GATnn'nnATC
Cuts at: 6 1632 3669 6
Size: 1626 2037 455
Fragments arranged by size:
2037 1626 455
BsaHI Gr'CG_yC
Cuts at: 582 935 1549 1797 2499 3995 582
Size: 353 614 248 702 1496 705
Fragments arranged by size:
1496 705 702 614 353 248
BsaWI w'CCGG_w
Cuts at: 1307 1828 2506 2780 2927 3338 1307
Size: 521 678 274 147 411 2087
Fragments arranged by size:
2087 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 780 1019 1093 780
Size: 239 74 3805
Fragments arranged by size:
3805 239 74
BseYI C'CCAG_C
Cuts at: 159 1543 2301 2878 3374 159
Size: 1384 758 577 496 903
Fragments arranged by size:
1384 903 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 257 1660 1706 2914 104
Size: 153 1403 46 1208 1308
Fragments arranged by size:
1403 1308 1208 153 46
BsiHKAI G_wGCw'C
Cuts at: 1132 1910 2100 2892 1132
Size: 778 190 792 2358
Fragments arranged by size:
2358 792 778 190
BsmI GAATG_Cn'
Cuts at: 460 460
Size: 4118
Bsp1286I G_dGCh'C
Cuts at: 640 712 1132 1743 1836 1910 2100 2162 2892
Size: 72 420 611 93 74 190 62 730
Cuts at: 2892 640
Size: 1866
Fragments arranged by size:
1866 730 611 420 190 93 74 72
62
BspEI T'CCGG_A
Cuts at: 3338 3338
Size: 4118
BspHI T'CATG_A
Cuts at: 3294 3294
Size: 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 160 1168 1382 1684 2065 160
Size: 1008 214 302 381 2213
Fragments arranged by size:
2213 1008 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2410 2464 3948 4113 50
Size: 2360 54 1484 165 55
Fragments arranged by size:
2360 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2030 2030
Size: 4118
BsrFI r'CCGG_y
Cuts at: 497 850 2116 2297 2506 497
Size: 353 1266 181 209 2109
Fragments arranged by size:
2109 1266 353 209 181
BssSI C'ACGA_G
Cuts at: 2389 2747 2389
Size: 358 3760
BstAPI GCAn_nnn'nTGC
Cuts at: 1495 1495
Size: 4118
BstBI TT'CG_AA
Cuts at: 2479 2479
Size: 4118
BstEII G'GTnAC_C
Cuts at: 112 549 112
Size: 437 3681
BstXI CCAn_nnnn'nTGG
Cuts at: 167 607 167
Size: 440 3678
BstYI r'GATC_y
Cuts at: 1 560 1143 1610 1633 1968 2214 3226 3312
Size: 559 583 467 23 335 246 1012 86
Cuts at: 3312 3324 3670 1
Size: 12 346 449
Fragments arranged by size:
1012 583 559 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 606 780 107
Size: 499 174 3445
Fragments arranged by size:
3445 499 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2068 2249 2412 2068
Size: 181 163 3774
Fragments arranged by size:
3774 181 163
BtsI GCAGTG_nn'
Cuts at: 193 520 1023 1361 193
Size: 327 503 338 2950
Fragments arranged by size:
2950 503 338 327
DraI TTT'AAA
Cuts at: 1117 1117
Size: 4118
DrdI GACnn_nn'nnGTC
Cuts at: 1003 1824 2682 1003
Size: 821 858 2439
Fragments arranged by size:
2439 858 821
EaeI y'GGCC_r
Cuts at: 417 744 1703 1877 2268 2295 3640 417
Size: 327 959 174 391 27 1345 895
Fragments arranged by size:
1345 959 895 391 327 174 27
EagI C'GGCC_G
Cuts at: 1703 1703
Size: 4118
EarI CTCTTCn'nnn_
Cuts at: 212 275 791 2141 2351 212
Size: 63 516 1350 210 1979
Fragments arranged by size:
1979 1350 516 210 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2326 2636 2782 3928 2326
Size: 310 146 1146 2516
Fragments arranged by size:
2516 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1130 1130
Size: 4118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 548 739 815 924 1943 2375 3122 3390
Size: 241 191 76 109 1019 432 747 268
Cuts at: 3390 307
Size: 1035
Fragments arranged by size:
1035 1019 747 432 268 241 191 109
76
EcoNI CCTnn'n_nnAGG
Cuts at: 232 373 232
Size: 141 3977
EcoO109I rG'GnC_Cy
Cuts at: 171 237 332 708 823 1257 171
Size: 66 95 376 115 434 3032
Fragments arranged by size:
3032 434 376 115 95 66
EcoRI G'AATT_C
Cuts at: 1122 1122
Size: 4118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 4118
FspI TGC'GCA
Cuts at: 1899 1899
Size: 4118
HaeII r_GCGC'y
Cuts at: 1552 1800 2822 3801 1552
Size: 248 1022 979 1869
Fragments arranged by size:
1869 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 224 256 1960 1990 1992 2022 224
Size: 32 1704 30 2 30 2320
Fragments arranged by size:
2320 1704 32 30 30 2
HincII GTy'rAC
Cuts at: 1157 1157
Size: 4118
KasI G'GCGC_C
Cuts at: 1548 1796 1548
Size: 248 3870
KpnI G_GTAC'C
Cuts at: 1138 1138
Size: 4118
MluI A'CGCG_T
Cuts at: 3551 3551
Size: 4118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2789 2973 2789
Size: 184 3934
MscI TGG'CCA
Cuts at: 1879 1879
Size: 4118
MslI CAynn'nnrTG
Cuts at: 256 605 929 956 2107 2234 256
Size: 349 324 27 1151 127 2140
Fragments arranged by size:
2140 1151 349 324 127 27
MspA1I CmG'CkG
Cuts at: 123 132 761 1229 1543 1903 2916 3161 123
Size: 9 629 468 314 360 1013 245 1080
Fragments arranged by size:
1080 1013 629 468 360 314 245 9
NaeI GCC'GGC
Cuts at: 499 2299 499
Size: 1800 2318
NarI GG'CG_CC
Cuts at: 1549 1797 1549
Size: 248 3870
NcoI C'CATG_G
Cuts at: 107 780 107
Size: 673 3445
NgoMIV G'CCGG_C
Cuts at: 497 2297 497
Size: 1800 2318
NsiI A_TGCA'T
Cuts at: 460 460
Size: 4118
NspI r_CATG'y
Cuts at: 186 922 1171 1671 2578 186
Size: 736 249 500 907 1726
Fragments arranged by size:
1726 907 736 500 249
PasI CC'CwG_GG
Cuts at: 228 228
Size: 4118
PciI A'CATG_T
Cuts at: 2574 2574
Size: 4118
PflMI CCAn_nnn'nTGG
Cuts at: 1088 1088
Size: 4118
PfoI T'CCnGG_A
Cuts at: 563 716 563
Size: 153 3965
PmeI GTTT'AAAC
Cuts at: 1117 1117
Size: 4118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3257 3289 3257
Size: 32 4086
PpuMI rG'GwC_Cy
Cuts at: 237 332 237
Size: 95 4023
PspOMI G'GGCC_C
Cuts at: 708 708
Size: 4118
PstI C_TGCA'G
Cuts at: 247 515 1165 1850 247
Size: 268 650 685 2515
Fragments arranged by size:
2515 685 650 268
PvuI CG_AT'CG
Cuts at: 104 257 104
Size: 153 3965
PvuII CAG'CTG
Cuts at: 761 1543 1903 761
Size: 782 360 2976
Fragments arranged by size:
2976 782 360
RsrII CG'GwC_CG
Cuts at: 2313 2313
Size: 4118
SacI G_AGCT'C
Cuts at: 1132 1132
Size: 4118
SalI G'TCGA_C
Cuts at: 1155 1155
Size: 4118
SapI GCTCTTCn'nnn_
Cuts at: 275 2141 2351 275
Size: 1866 210 2042
Fragments arranged by size:
2042 1866 210
SbfI CC_TGCA'GG
Cuts at: 1165 1165
Size: 4118
ScaI AGT'ACT
Cuts at: 3635 3635
Size: 4118
SfcI C'TryA_G
Cuts at: 33 243 511 1161 1846 2839 3030 33
Size: 210 268 650 685 993 191 1121
Fragments arranged by size:
1121 993 685 650 268 210 191
SfoI GGC'GCC
Cuts at: 1550 1798 1550
Size: 248 3870
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
SmaI CCC'GGG
Cuts at: 635 1140 635
Size: 505 3613
SmlI C'TyrA_G
Cuts at: 1273 2188 2680 2942 1273
Size: 915 492 262 2449
Fragments arranged by size:
2449 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4118
SphI G_CATG'C
Cuts at: 186 1171 1671 186
Size: 985 500 2633
Fragments arranged by size:
2633 985 500
SrfI GCCC'GGGC
Cuts at: 635 635
Size: 4118
StyI C'CwwG_G
Cuts at: 107 780 1252 1606 107
Size: 673 472 354 2619
Fragments arranged by size:
2619 673 472 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 793 919 1007 1701 1834 2293 2502 793
Size: 126 88 694 133 459 209 2409
Fragments arranged by size:
2409 694 459 209 133 126 88
TatI w'GTAC_w
Cuts at: 970 3633 970
Size: 2663 1455
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4086
Tth111I GACn'n_nGTC
Cuts at: 1915 1915
Size: 4118
XbaI T'CTAG_A
Cuts at: 1149 1149
Size: 4118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 412 1015 1613 412
Size: 603 598 2917
Fragments arranged by size:
2917 603 598
XmaI C'CCGG_G
Cuts at: 633 1138 633
Size: 505 3613
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BcgI BglI BmgBI BmtI BsaXI BsgI BsiWI
BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SspI StuI
SwaI XhoI XmnI ZraI