Restriction map for
pF1KB6408
(Circular) (Six-base) MAPSORT of: pF1KB6408.seq Check: 5479 from: 1 to: 4118
pF1KB6408 4118 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 1156 1156
Size: 4118
Acc65I G'GTAC_C
Cuts at: 458 1134 458
Size: 676 3442
AclI AA'CG_TT
Cuts at: 3958 3958
Size: 4118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 756 961 1943 2375 3122 3390 756
Size: 205 982 432 747 268 1484
Fragments arranged by size:
1484 982 747 432 268 205
AfeI AGC'GCT
Cuts at: 496 496
Size: 4118
AflIII A'CryG_T
Cuts at: 1470 2574 3551 1470
Size: 1104 977 2037
Fragments arranged by size:
2037 1104 977
AgeI A'CCGG_T
Cuts at: 2506 2506
Size: 4118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 253 253
Size: 4118
AlwNI CAG_nnn'CTG
Cuts at: 1045 1057 1423 2990 1045
Size: 12 366 1567 2173
Fragments arranged by size:
2173 1567 366 12
ApaLI G'TGCA_C
Cuts at: 2888 2888
Size: 4118
ApoI r'AATT_y
Cuts at: 1071 1122 3471 3727 1071
Size: 51 2349 256 1462
Fragments arranged by size:
2349 1462 256 51
AseI AT'TA_AT
Cuts at: 20 20
Size: 4118
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
AvaI C'yCGr_G
Cuts at: 1138 1138
Size: 4118
BamHI G'GATC_C
Cuts at: 111 1143 111
Size: 1032 3086
BanI G'GyrC_C
Cuts at: 458 649 1134 1548 1796 1831 458
Size: 191 485 414 248 35 2745
Fragments arranged by size:
2745 485 414 248 191 35
BanII G_rGCy'C
Cuts at: 125 1132 2162 125
Size: 1007 1030 2081
Fragments arranged by size:
2081 1030 1007
BbeI G_GCGC'C
Cuts at: 1552 1800 1552
Size: 248 3870
BciVI GTATCCnnnnn_n'
Cuts at: 419 2016 2777 4118 419
Size: 1597 761 1341 419
Fragments arranged by size:
1597 1341 761 419
BclI T'GATC_A
Cuts at: 1638 1638
Size: 4118
BglII A'GATC_T
Cuts at: 1633 3670 1633
Size: 2037 2081
BlpI GC'TnA_GC
Cuts at: 1230 1230
Size: 4118
Bme1580I G_kGCm'C
Cuts at: 652 1743 1836 2892 652
Size: 1091 93 1056 1878
Fragments arranged by size:
1878 1091 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 601 1330 1411 1746 3890 601
Size: 729 81 335 2144 829
Fragments arranged by size:
2144 829 729 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 546 2485 546
Size: 1939 2179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 427 493 521 654 337
Size: 90 66 28 133 3801
Fragments arranged by size:
3801 133 90 66 28
Bpu10I CC'TnA_GC
Cuts at: 1004 1004
Size: 4118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1294 2173 2665 2963 1294
Size: 879 492 298 2449
Fragments arranged by size:
2449 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 534 3822 534
Size: 3288 830
BsaAI yAC'GTr
Cuts at: 1471 1471
Size: 4118
BsaBI GATnn'nnATC
Cuts at: 6 862 1632 3669 6
Size: 856 770 2037 455
Fragments arranged by size:
2037 856 770 455
BsaHI Gr'CG_yC
Cuts at: 1549 1797 2499 3995 1549
Size: 248 702 1496 1672
Fragments arranged by size:
1672 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1307 1828 2506 2780 2927 3338 1307
Size: 521 678 274 147 411 2087
Fragments arranged by size:
2087 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 115 115
Size: 4118
BseYI C'CCAG_C
Cuts at: 666 707 832 999 1543 2301 2878 3374 666
Size: 41 125 167 544 758 577 496 1410
Fragments arranged by size:
1410 758 577 544 496 167 125 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 564 582 717 564
Size: 18 135 3965
Fragments arranged by size:
3965 135 18
BsiEI CG_ry'CG
Cuts at: 104 1660 1706 2914 104
Size: 1556 46 1208 1308
Fragments arranged by size:
1556 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1132 1910 2100 2892 1132
Size: 778 190 792 2358
Fragments arranged by size:
2358 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 125 652 1132 1743 1836 1910 2100 2162 2892
Size: 527 480 611 93 74 190 62 730
Cuts at: 2892 125
Size: 1351
Fragments arranged by size:
1351 730 611 527 480 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3338 3338
Size: 4118
BspHI T'CATG_A
Cuts at: 854 3294 854
Size: 2440 1678
BspMI ACCTGCnnnn'nnnn_
Cuts at: 516 586 1168 1382 1684 2065 516
Size: 70 582 214 302 381 2569
Fragments arranged by size:
2569 582 381 302 214 70
BsrBI CCG'CTC
Cuts at: 50 2410 2464 3948 4113 50
Size: 2360 54 1484 165 55
Fragments arranged by size:
2360 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2030 2030
Size: 4118
BsrFI r'CCGG_y
Cuts at: 2116 2297 2506 2116
Size: 181 209 3728
Fragments arranged by size:
3728 209 181
BssSI C'ACGA_G
Cuts at: 2389 2747 2389
Size: 358 3760
BstAPI GCAn_nnn'nTGC
Cuts at: 829 1495 829
Size: 666 3452
BstBI TT'CG_AA
Cuts at: 2479 2479
Size: 4118
BstYI r'GATC_y
Cuts at: 1 111 1143 1610 1633 1968 2214 3226 3312
Size: 110 1032 467 23 335 246 1012 86
Cuts at: 3312 3324 3670 1
Size: 12 346 449
Fragments arranged by size:
1032 1012 467 449 346 335 246 110
86 23 12
BtgI C'CryG_G
Cuts at: 107 778 817 107
Size: 671 39 3408
Fragments arranged by size:
3408 671 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2068 2249 2412 2068
Size: 181 163 3774
Fragments arranged by size:
3774 181 163
BtsI GCAGTG_nn'
Cuts at: 830 1361 830
Size: 531 3587
DraI TTT'AAA
Cuts at: 554 1117 554
Size: 563 3555
DrdI GACnn_nn'nnGTC
Cuts at: 1824 2682 1824
Size: 858 3260
EaeI y'GGCC_r
Cuts at: 1703 1877 2268 2295 3640 1703
Size: 174 391 27 1345 2181
Fragments arranged by size:
2181 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1703 1703
Size: 4118
EarI CTCTTCn'nnn_
Cuts at: 888 980 2141 2351 888
Size: 92 1161 210 2655
Fragments arranged by size:
2655 1161 210 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2326 2636 2782 3928 2326
Size: 310 146 1146 2516
Fragments arranged by size:
2516 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1130 1130
Size: 4118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 427 493 521 654 756 961 1943 2375
Size: 90 66 28 133 102 205 982 432
Cuts at: 2375 3122 3390 337
Size: 747 268 1065
Fragments arranged by size:
1065 982 747 432 268 205 133 102
90 66 28
EcoO109I rG'GnC_Cy
Cuts at: 367 575 970 1008 1257 367
Size: 208 395 38 249 3228
Fragments arranged by size:
3228 395 249 208 38
EcoRI G'AATT_C
Cuts at: 1122 1122
Size: 4118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 4118
FspI TGC'GCA
Cuts at: 1899 1899
Size: 4118
HaeII r_GCGC'y
Cuts at: 498 1552 1800 2822 3801 498
Size: 1054 248 1022 979 815
Fragments arranged by size:
1054 1022 979 815 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1960 1990 1992 2022 1960
Size: 30 2 30 4056
Fragments arranged by size:
4056 30 30 2
HincII GTy'rAC
Cuts at: 1157 1157
Size: 4118
KasI G'GCGC_C
Cuts at: 1548 1796 1548
Size: 248 3870
KpnI G_GTAC'C
Cuts at: 462 1138 462
Size: 676 3442
MluI A'CGCG_T
Cuts at: 3551 3551
Size: 4118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2789 2973 2789
Size: 184 3934
MscI TGG'CCA
Cuts at: 1879 1879
Size: 4118
MslI CAynn'nnrTG
Cuts at: 868 2107 2234 868
Size: 1239 127 2752
Fragments arranged by size:
2752 1239 127
MspA1I CmG'CkG
Cuts at: 632 1229 1543 1903 2916 3161 632
Size: 597 314 360 1013 245 1589
Fragments arranged by size:
1589 1013 597 360 314 245
NaeI GCC'GGC
Cuts at: 2299 2299
Size: 4118
NarI GG'CG_CC
Cuts at: 1549 1797 1549
Size: 248 3870
NcoI C'CATG_G
Cuts at: 107 817 107
Size: 710 3408
NdeI CA'TA_TG
Cuts at: 252 252
Size: 4118
NgoMIV G'CCGG_C
Cuts at: 2297 2297
Size: 4118
NspI r_CATG'y
Cuts at: 715 1171 1671 2578 715
Size: 456 500 907 2255
Fragments arranged by size:
2255 907 500 456
PasI CC'CwG_GG
Cuts at: 420 420
Size: 4118
PciI A'CATG_T
Cuts at: 2574 2574
Size: 4118
PfoI T'CCnGG_A
Cuts at: 1061 1061
Size: 4118
PmeI GTTT'AAAC
Cuts at: 1117 1117
Size: 4118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3257 3289 3257
Size: 32 4086
PpuMI rG'GwC_Cy
Cuts at: 575 970 1008 575
Size: 395 38 3685
Fragments arranged by size:
3685 395 38
PstI C_TGCA'G
Cuts at: 509 1165 1850 509
Size: 656 685 2777
Fragments arranged by size:
2777 685 656
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4118
PvuII CAG'CTG
Cuts at: 632 1543 1903 632
Size: 911 360 2847
Fragments arranged by size:
2847 911 360
RsrII CG'GwC_CG
Cuts at: 2313 2313
Size: 4118
SacI G_AGCT'C
Cuts at: 1132 1132
Size: 4118
SalI G'TCGA_C
Cuts at: 1155 1155
Size: 4118
SapI GCTCTTCn'nnn_
Cuts at: 2141 2351 2141
Size: 210 3908
SbfI CC_TGCA'GG
Cuts at: 1165 1165
Size: 4118
ScaI AGT'ACT
Cuts at: 3635 3635
Size: 4118
SexAI A'CCwGG_T
Cuts at: 966 966
Size: 4118
SfcI C'TryA_G
Cuts at: 33 505 880 1161 1846 2839 3030 33
Size: 472 375 281 685 993 191 1121
Fragments arranged by size:
1121 993 685 472 375 281 191
SfoI GGC'GCC
Cuts at: 1550 1798 1550
Size: 248 3870
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
SmaI CCC'GGG
Cuts at: 1140 1140
Size: 4118
SmlI C'TyrA_G
Cuts at: 1273 2188 2680 2942 1273
Size: 915 492 262 2449
Fragments arranged by size:
2449 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4118
SphI G_CATG'C
Cuts at: 715 1171 1671 715
Size: 456 500 3162
Fragments arranged by size:
3162 500 456
StyI C'CwwG_G
Cuts at: 107 116 817 1252 1606 107
Size: 9 701 435 354 2619
Fragments arranged by size:
2619 701 435 354 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 667 671 1701 1834 2293 2502 667
Size: 4 1030 133 459 209 2283
Fragments arranged by size:
2283 1030 459 209 133 4
TatI w'GTAC_w
Cuts at: 3633 3633
Size: 4118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 218 250 673 705 3635 3667 218
Size: 32 423 32 2930 32 669
Fragments arranged by size:
2930 669 423 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1915 1915
Size: 4118
XbaI T'CTAG_A
Cuts at: 1149 1149
Size: 4118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1613 1613
Size: 4118
XmaI C'CCGG_G
Cuts at: 1138 1138
Size: 4118
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI