Restriction map for
pF1KB6405
(Circular) (Six-base) MAPSORT of: pF1KB6405.seq Check: 2500 from: 1 to: 4028
pF1KB6405 4028 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AatII G_ACGT'C
Cuts at: 845 845
Size: 4028
AccI GT'mk_AC
Cuts at: 557 926 1066 557
Size: 369 140 3519
Fragments arranged by size:
3519 369 140
Acc65I G'GTAC_C
Cuts at: 1044 1044
Size: 4028
AclI AA'CG_TT
Cuts at: 3868 3868
Size: 4028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 719 1853 2285 3032 3300 719
Size: 1134 432 747 268 1447
Fragments arranged by size:
1447 1134 747 432 268
AflIII A'CryG_T
Cuts at: 1380 2484 3461 1380
Size: 1104 977 1947
Fragments arranged by size:
1947 1104 977
AgeI A'CCGG_T
Cuts at: 2416 2416
Size: 4028
AleI CACnn'nnGTG
Cuts at: 637 637
Size: 4028
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 465 465
Size: 4028
AlwNI CAG_nnn'CTG
Cuts at: 181 240 1333 2900 181
Size: 59 1093 1567 1309
Fragments arranged by size:
1567 1309 1093 59
ApaLI G'TGCA_C
Cuts at: 2798 2798
Size: 4028
ApoI r'AATT_y
Cuts at: 1032 3381 3637 1032
Size: 2349 256 1423
Fragments arranged by size:
2349 1423 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4028
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
AvaI C'yCGr_G
Cuts at: 1048 1048
Size: 4028
BamHI G'GATC_C
Cuts at: 1053 1053
Size: 4028
BanI G'GyrC_C
Cuts at: 1044 1458 1706 1741 1044
Size: 414 248 35 3331
Fragments arranged by size:
3331 414 248 35
BanII G_rGCy'C
Cuts at: 320 1007 1042 2072 320
Size: 687 35 1030 2276
Fragments arranged by size:
2276 1030 687 35
BbeI G_GCGC'C
Cuts at: 1462 1710 1462
Size: 248 3780
BbvCI CC'TCA_GC
Cuts at: 171 987 171
Size: 816 3212
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 916 950 916
Size: 34 3994
BciVI GTATCCnnnnn_n'
Cuts at: 1926 2687 4028 1926
Size: 761 1341 1926
Fragments arranged by size:
1926 1341 761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4028
BglI GCCn_nnn'nGGC
Cuts at: 535 535
Size: 4028
BglII A'GATC_T
Cuts at: 444 678 1543 3580 444
Size: 234 865 2037 892
Fragments arranged by size:
2037 892 865 234
BlpI GC'TnA_GC
Cuts at: 1140 1140
Size: 4028
Bme1580I G_kGCm'C
Cuts at: 1653 1746 2802 1653
Size: 93 1056 2879
Fragments arranged by size:
2879 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1240 1321 1656 3800 1240
Size: 81 335 2144 1468
Fragments arranged by size:
2144 1468 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 4028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 483 220
Size: 263 3765
Bpu10I CC'TnA_GC
Cuts at: 171 987 171
Size: 816 3212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 1204 2083 2575 2873 862
Size: 342 879 492 298 2017
Fragments arranged by size:
2017 879 492 342 298
BsaI GGTCTCn'nnnn_
Cuts at: 3732 3732
Size: 4028
BsaAI yAC'GTr
Cuts at: 1381 1381
Size: 4028
BsaBI GATnn'nnATC
Cuts at: 6 1542 3579 6
Size: 1536 2037 455
Fragments arranged by size:
2037 1536 455
BsaHI Gr'CG_yC
Cuts at: 842 1459 1707 2409 3905 842
Size: 617 248 702 1496 965
Fragments arranged by size:
1496 965 702 617 248
BsaWI w'CCGG_w
Cuts at: 188 1217 1738 2416 2690 2837 3248 188
Size: 1029 521 678 274 147 411 968
Fragments arranged by size:
1029 968 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 481 511 904 934 481
Size: 30 393 30 3575
Fragments arranged by size:
3575 393 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 279 925 279
Size: 646 3382
BseYI C'CCAG_C
Cuts at: 1453 2211 2788 3284 1453
Size: 758 577 496 2197
Fragments arranged by size:
2197 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1570 1616 2824 104
Size: 1466 46 1208 1308
Fragments arranged by size:
1466 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 320 1007 1042 1820 2010 2802 320
Size: 687 35 778 190 792 1546
Fragments arranged by size:
1546 792 778 687 190 35
Bsp1286I G_dGCh'C
Cuts at: 320 1007 1042 1653 1746 1820 2010 2072 2802
Size: 687 35 611 93 74 190 62 730
Cuts at: 2802 320
Size: 1546
Fragments arranged by size:
1546 730 687 611 190 93 74 62
35
BspEI T'CCGG_A
Cuts at: 188 3248 188
Size: 3060 968
BspHI T'CATG_A
Cuts at: 383 3204 383
Size: 2821 1207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1078 1292 1594 1975 1078
Size: 214 302 381 3131
Fragments arranged by size:
3131 381 302 214
BsrBI CCG'CTC
Cuts at: 50 393 2320 2374 3858 4023 50
Size: 343 1927 54 1484 165 55
Fragments arranged by size:
1927 1484 343 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1940 1940
Size: 4028
BsrFI r'CCGG_y
Cuts at: 2026 2207 2416 2026
Size: 181 209 3638
Fragments arranged by size:
3638 209 181
BssSI C'ACGA_G
Cuts at: 2299 2657 2299
Size: 358 3670
BstAPI GCAn_nnn'nTGC
Cuts at: 181 1405 181
Size: 1224 2804
BstBI TT'CG_AA
Cuts at: 2389 2389
Size: 4028
BstXI CCAn_nnnn'nTGG
Cuts at: 750 750
Size: 4028
BstYI r'GATC_y
Cuts at: 1 156 444 678 1053 1520 1543 1878 2124
Size: 155 288 234 375 467 23 335 246
Cuts at: 2124 3136 3222 3234 3580 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 375 346 335 288 246
234 155 86 23 12
BstZ17I GTA'TAC
Cuts at: 558 558
Size: 4028
BtgI C'CryG_G
Cuts at: 107 530 919 107
Size: 423 389 3216
Fragments arranged by size:
3216 423 389
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1013 1978 2159 2322 1013
Size: 965 181 163 2719
Fragments arranged by size:
2719 965 181 163
BtsI GCAGTG_nn'
Cuts at: 182 305 932 1271 182
Size: 123 627 339 2939
Fragments arranged by size:
2939 627 339 123
DraI TTT'AAA
Cuts at: 1027 1027
Size: 4028
DrdI GACnn_nn'nnGTC
Cuts at: 1734 2592 1734
Size: 858 3170
EaeI y'GGCC_r
Cuts at: 536 599 1613 1787 2178 2205 3550 536
Size: 63 1014 174 391 27 1345 1014
Fragments arranged by size:
1345 1014 1014 391 174 63 27
EagI C'GGCC_G
Cuts at: 1613 1613
Size: 4028
EarI CTCTTCn'nnn_
Cuts at: 205 325 2051 2261 205
Size: 120 1726 210 1972
Fragments arranged by size:
1972 1726 210 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2236 2546 2692 3838 2236
Size: 310 146 1146 2426
Fragments arranged by size:
2426 1146 310 146
EcoICRI GAG'CTC
Cuts at: 318 1005 1040 318
Size: 687 35 3306
Fragments arranged by size:
3306 687 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 483 719 1853 2285 3032 3300 220
Size: 263 236 1134 432 747 268 948
Fragments arranged by size:
1134 948 747 432 268 263 236
EcoNI CCTnn'n_nnAGG
Cuts at: 907 907
Size: 4028
EcoO109I rG'GnC_Cy
Cuts at: 1167 1167
Size: 4028
EcoRI G'AATT_C
Cuts at: 1032 1032
Size: 4028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 697 2047 697
Size: 1350 2678
FspI TGC'GCA
Cuts at: 1809 1809
Size: 4028
HaeII r_GCGC'y
Cuts at: 1462 1710 2732 3711 1462
Size: 248 1022 979 1779
Fragments arranged by size:
1779 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 154 186 234 266 887 919 1870 1900 1902
Size: 32 48 32 621 32 951 30 2
Cuts at: 1902 1932 154
Size: 30 2250
Fragments arranged by size:
2250 951 621 48 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 927 1067 927
Size: 140 3888
KasI G'GCGC_C
Cuts at: 1458 1706 1458
Size: 248 3780
KpnI G_GTAC'C
Cuts at: 1048 1048
Size: 4028
MluI A'CGCG_T
Cuts at: 3461 3461
Size: 4028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2699 2883 2699
Size: 184 3844
MscI TGG'CCA
Cuts at: 601 1789 601
Size: 1188 2840
MslI CAynn'nnrTG
Cuts at: 637 2017 2144 637
Size: 1380 127 2521
Fragments arranged by size:
2521 1380 127
MspA1I CmG'CkG
Cuts at: 1139 1453 1813 2826 3071 1139
Size: 314 360 1013 245 2096
Fragments arranged by size:
2096 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2209 2209
Size: 4028
NarI GG'CG_CC
Cuts at: 1459 1707 1459
Size: 248 3780
NcoI C'CATG_G
Cuts at: 107 107
Size: 4028
NgoMIV G'CCGG_C
Cuts at: 2207 2207
Size: 4028
NspI r_CATG'y
Cuts at: 1081 1581 2488 1081
Size: 500 907 2621
Fragments arranged by size:
2621 907 500
PciI A'CATG_T
Cuts at: 2484 2484
Size: 4028
PfoI T'CCnGG_A
Cuts at: 518 518
Size: 4028
PmeI GTTT'AAAC
Cuts at: 1027 1027
Size: 4028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 392 424 3167 3199 392
Size: 32 2743 32 1221
Fragments arranged by size:
2743 1221 32 32
PstI C_TGCA'G
Cuts at: 1075 1760 1075
Size: 685 3343
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4028
PvuII CAG'CTG
Cuts at: 1453 1813 1453
Size: 360 3668
RsrII CG'GwC_CG
Cuts at: 2223 2223
Size: 4028
SacI G_AGCT'C
Cuts at: 320 1007 1042 320
Size: 687 35 3306
Fragments arranged by size:
3306 687 35
SalI G'TCGA_C
Cuts at: 925 1065 925
Size: 140 3888
SapI GCTCTTCn'nnn_
Cuts at: 325 2051 2261 325
Size: 1726 210 2092
Fragments arranged by size:
2092 1726 210
SbfI CC_TGCA'GG
Cuts at: 1075 1075
Size: 4028
ScaI AGT'ACT
Cuts at: 3545 3545
Size: 4028
SfcI C'TryA_G
Cuts at: 33 1071 1756 2749 2940 33
Size: 1038 685 993 191 1121
Fragments arranged by size:
1121 1038 993 685 191
SfoI GGC'GCC
Cuts at: 1460 1708 1460
Size: 248 3780
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
SmaI CCC'GGG
Cuts at: 1050 1050
Size: 4028
SmlI C'TyrA_G
Cuts at: 877 1183 2098 2590 2852 877
Size: 306 915 492 262 2053
Fragments arranged by size:
2053 915 492 306 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4028
SphI G_CATG'C
Cuts at: 1081 1581 1081
Size: 500 3528
SspI AAT'ATT
Cuts at: 482 482
Size: 4028
StyI C'CwwG_G
Cuts at: 107 1162 1516 107
Size: 1055 354 2619
Fragments arranged by size:
2619 1055 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1611 1744 2203 2412 1611
Size: 133 459 209 3227
Fragments arranged by size:
3227 459 209 133
TatI w'GTAC_w
Cuts at: 3543 3543
Size: 4028
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 481 513 904 936 3545 3577 481
Size: 32 391 32 2609 32 932
Fragments arranged by size:
2609 932 391 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1825 1825
Size: 4028
XbaI T'CTAG_A
Cuts at: 1059 1059
Size: 4028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1523 1523
Size: 4028
XmaI C'CCGG_G
Cuts at: 1048 1048
Size: 4028
XmnI GAAnn'nnTTC
Cuts at: 404 404
Size: 4028
ZraI GAC'GTC
Cuts at: 843 843
Size: 4028
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI