Restriction map for
pF1KB6402
(Circular) (Six-base) MAPSORT of: pF1KB6402.seq Check: 22 from: 1 to: 4112
pF1KB6402 4112 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 366 366
Size: 4112
AccI GT'mk_AC
Cuts at: 608 1150 608
Size: 542 3570
Acc65I G'GTAC_C
Cuts at: 1128 1128
Size: 4112
AclI AA'CG_TT
Cuts at: 3952 3952
Size: 4112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1937 2369 3116 3384 1937
Size: 432 747 268 2665
Fragments arranged by size:
2665 747 432 268
AflIII A'CryG_T
Cuts at: 1464 2568 3545 1464
Size: 1104 977 2031
Fragments arranged by size:
2031 1104 977
AgeI A'CCGG_T
Cuts at: 2500 2500
Size: 4112
AlwNI CAG_nnn'CTG
Cuts at: 603 1417 2984 603
Size: 814 1567 1731
Fragments arranged by size:
1731 1567 814
ApaI G_GGCC'C
Cuts at: 839 839
Size: 4112
ApaLI G'TGCA_C
Cuts at: 2882 2882
Size: 4112
ApoI r'AATT_y
Cuts at: 367 1116 3465 3721 367
Size: 749 2349 256 758
Fragments arranged by size:
2349 758 749 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4112
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
AvaI C'yCGr_G
Cuts at: 1132 1132
Size: 4112
BamHI G'GATC_C
Cuts at: 1137 1137
Size: 4112
BanI G'GyrC_C
Cuts at: 1128 1542 1790 1825 1128
Size: 414 248 35 3415
Fragments arranged by size:
3415 414 248 35
BanII G_rGCy'C
Cuts at: 839 1126 2156 839
Size: 287 1030 2795
Fragments arranged by size:
2795 1030 287
BbeI G_GCGC'C
Cuts at: 1546 1794 1546
Size: 248 3864
BbsI GAAGACnn'nnnn_
Cuts at: 929 929
Size: 4112
BbvCI CC'TCA_GC
Cuts at: 153 1083 153
Size: 930 3182
BciVI GTATCCnnnnn_n'
Cuts at: 2010 2771 4112 2010
Size: 761 1341 2010
Fragments arranged by size:
2010 1341 761
BclI T'GATC_A
Cuts at: 171 1632 171
Size: 1461 2651
BglII A'GATC_T
Cuts at: 454 1627 3664 454
Size: 1173 2037 902
Fragments arranged by size:
2037 1173 902
BlpI GC'TnA_GC
Cuts at: 1224 1224
Size: 4112
Bme1580I G_kGCm'C
Cuts at: 839 1737 1830 2886 839
Size: 898 93 1056 2065
Fragments arranged by size:
2065 1056 898 93
BmrI ACTGGGnnnn_n'
Cuts at: 581 1324 1405 1740 3884 581
Size: 743 81 335 2144 809
Fragments arranged by size:
2144 809 743 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 4112
Bpu10I CC'TnA_GC
Cuts at: 153 794 1083 153
Size: 641 289 3182
Fragments arranged by size:
3182 641 289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 608 1288 2167 2659 2957 608
Size: 680 879 492 298 1763
Fragments arranged by size:
1763 879 680 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3816 3816
Size: 4112
BsaAI yAC'GTr
Cuts at: 483 1041 1465 483
Size: 558 424 3130
Fragments arranged by size:
3130 558 424
BsaBI GATnn'nnATC
Cuts at: 6 1626 3663 6
Size: 1620 2037 455
Fragments arranged by size:
2037 1620 455
BsaHI Gr'CG_yC
Cuts at: 1543 1791 2493 3989 1543
Size: 248 702 1496 1666
Fragments arranged by size:
1666 1496 702 248
BsaWI w'CCGG_w
Cuts at: 563 1058 1301 1822 2500 2774 2921 3332 563
Size: 495 243 521 678 274 147 411 1343
Fragments arranged by size:
1343 678 521 495 411 274 243 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 264 264
Size: 4112
BseYI C'CCAG_C
Cuts at: 1010 1537 2295 2872 3368 1010
Size: 527 758 577 496 1754
Fragments arranged by size:
1754 758 577 527 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 274
Size: 4112
BsiEI CG_ry'CG
Cuts at: 104 1654 1700 2908 104
Size: 1550 46 1208 1308
Fragments arranged by size:
1550 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 546 1126 1904 2094 2886 546
Size: 580 778 190 792 1772
Fragments arranged by size:
1772 792 778 580 190
Bsp1286I G_dGCh'C
Cuts at: 546 839 1126 1737 1830 1904 2094 2156 2886
Size: 293 287 611 93 74 190 62 730
Cuts at: 2886 546
Size: 1772
Fragments arranged by size:
1772 730 611 293 287 190 93 74
62
BspEI T'CCGG_A
Cuts at: 563 3332 563
Size: 2769 1343
BspHI T'CATG_A
Cuts at: 864 3288 864
Size: 2424 1688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 366 850 1162 1376 1678 2059 366
Size: 484 312 214 302 381 2419
Fragments arranged by size:
2419 484 381 312 302 214
BsrBI CCG'CTC
Cuts at: 50 2404 2458 3942 4107 50
Size: 2354 54 1484 165 55
Fragments arranged by size:
2354 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 636 648 2024 636
Size: 12 1376 2724
Fragments arranged by size:
2724 1376 12
BsrFI r'CCGG_y
Cuts at: 775 2110 2291 2500 775
Size: 1335 181 209 2387
Fragments arranged by size:
2387 1335 209 181
BssSI C'ACGA_G
Cuts at: 2383 2741 2383
Size: 358 3754
BstAPI GCAn_nnn'nTGC
Cuts at: 1489 1489
Size: 4112
BstBI TT'CG_AA
Cuts at: 2473 2473
Size: 4112
BstXI CCAn_nnnn'nTGG
Cuts at: 459 459
Size: 4112
BstYI r'GATC_y
Cuts at: 1 454 1137 1604 1627 1962 2208 3220 3306
Size: 453 683 467 23 335 246 1012 86
Cuts at: 3306 3318 3664 1
Size: 12 346 449
Fragments arranged by size:
1012 683 467 453 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 223 2062 2243 2406 223
Size: 1839 181 163 1929
Fragments arranged by size:
1929 1839 181 163
BtsI GCAGTG_nn'
Cuts at: 263 924 1355 263
Size: 661 431 3020
Fragments arranged by size:
3020 661 431
DraI TTT'AAA
Cuts at: 1111 1111
Size: 4112
DraIII CAC_nnn'GTG
Cuts at: 592 592
Size: 4112
DrdI GACnn_nn'nnGTC
Cuts at: 1818 2676 1818
Size: 858 3254
EaeI y'GGCC_r
Cuts at: 332 1049 1697 1871 2262 2289 3634 332
Size: 717 648 174 391 27 1345 810
Fragments arranged by size:
1345 810 717 648 391 174 27
EagI C'GGCC_G
Cuts at: 1697 1697
Size: 4112
EarI CTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2320 2630 2776 3922 2320
Size: 310 146 1146 2510
Fragments arranged by size:
2510 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1124 1124
Size: 4112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1937 2369 3116 3384 1937
Size: 432 747 268 2665
Fragments arranged by size:
2665 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 814 814
Size: 4112
EcoO109I rG'GnC_Cy
Cuts at: 447 807 835 1251 447
Size: 360 28 416 3308
Fragments arranged by size:
3308 416 360 28
EcoRI G'AATT_C
Cuts at: 367 1116 367
Size: 749 3363
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 4112
FspI TGC'GCA
Cuts at: 1893 1893
Size: 4112
HaeII r_GCGC'y
Cuts at: 1546 1794 2816 3795 1546
Size: 248 1022 979 1863
Fragments arranged by size:
1863 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1954 1984 1986 2016 1954
Size: 30 2 30 4050
Fragments arranged by size:
4050 30 30 2
HincII GTy'rAC
Cuts at: 1151 1151
Size: 4112
KasI G'GCGC_C
Cuts at: 1542 1790 1542
Size: 248 3864
KpnI G_GTAC'C
Cuts at: 1132 1132
Size: 4112
MluI A'CGCG_T
Cuts at: 3545 3545
Size: 4112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2783 2967 2783
Size: 184 3928
MscI TGG'CCA
Cuts at: 334 1051 1873 334
Size: 717 822 2573
Fragments arranged by size:
2573 822 717
MslI CAynn'nnrTG
Cuts at: 277 2101 2228 277
Size: 1824 127 2161
Fragments arranged by size:
2161 1824 127
MspA1I CmG'CkG
Cuts at: 1010 1223 1537 1897 2910 3155 1010
Size: 213 314 360 1013 245 1967
Fragments arranged by size:
1967 1013 360 314 245 213
NaeI GCC'GGC
Cuts at: 2293 2293
Size: 4112
NarI GG'CG_CC
Cuts at: 1543 1791 1543
Size: 248 3864
NdeI CA'TA_TG
Cuts at: 717 717
Size: 4112
NgoMIV G'CCGG_C
Cuts at: 2291 2291
Size: 4112
NspI r_CATG'y
Cuts at: 1165 1665 2572 1165
Size: 500 907 2705
Fragments arranged by size:
2705 907 500
PciI A'CATG_T
Cuts at: 2568 2568
Size: 4112
PfoI T'CCnGG_A
Cuts at: 425 425
Size: 4112
PmeI GTTT'AAAC
Cuts at: 1111 1111
Size: 4112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 4080
PspOMI G'GGCC_C
Cuts at: 835 835
Size: 4112
PstI C_TGCA'G
Cuts at: 1159 1844 1159
Size: 685 3427
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4112
PvuII CAG'CTG
Cuts at: 1010 1537 1897 1010
Size: 527 360 3225
Fragments arranged by size:
3225 527 360
RsrII CG'GwC_CG
Cuts at: 2307 2307
Size: 4112
SacI G_AGCT'C
Cuts at: 1126 1126
Size: 4112
SalI G'TCGA_C
Cuts at: 1149 1149
Size: 4112
SapI GCTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
SbfI CC_TGCA'GG
Cuts at: 1159 1159
Size: 4112
ScaI AGT'ACT
Cuts at: 3629 3629
Size: 4112
SfcI C'TryA_G
Cuts at: 33 912 1155 1840 2833 3024 33
Size: 879 243 685 993 191 1121
Fragments arranged by size:
1121 993 879 685 243 191
SfoI GGC'GCC
Cuts at: 1544 1792 1544
Size: 248 3864
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
SmaI CCC'GGG
Cuts at: 1134 1134
Size: 4112
SmlI C'TyrA_G
Cuts at: 623 1267 2182 2674 2936 623
Size: 644 915 492 262 1799
Fragments arranged by size:
1799 915 644 492 262
SnaBI TAC'GTA
Cuts at: 1041 1041
Size: 4112
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4112
SphI G_CATG'C
Cuts at: 1165 1665 1165
Size: 500 3612
SspI AAT'ATT
Cuts at: 991 991
Size: 4112
StuI AGG'CCT
Cuts at: 692 692
Size: 4112
StyI C'CwwG_G
Cuts at: 1246 1600 1246
Size: 354 3758
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1021 1695 1828 2287 2496 1021
Size: 674 133 459 209 2637
Fragments arranged by size:
2637 674 459 209 133
TatI w'GTAC_w
Cuts at: 743 3627 743
Size: 2884 1228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3629 3661 3629
Size: 32 4080
Tth111I GACn'n_nGTC
Cuts at: 1909 1909
Size: 4112
XbaI T'CTAG_A
Cuts at: 1143 1143
Size: 4112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1607 1607
Size: 4112
XmaI C'CCGG_G
Cuts at: 1132 1132
Size: 4112
XmnI GAAnn'nnTTC
Cuts at: 421 421
Size: 4112
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PfoI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BpmI
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SwaI XhoI
ZraI