Restriction map for
pF1KB6389
(Circular) (Six-base) MAPSORT of: pF1KB6389.seq Check: 2380 from: 1 to: 3938
pF1KB6389 3938 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 397 397
Size: 3938
AccI GT'mk_AC
Cuts at: 976 976
Size: 3938
Acc65I G'GTAC_C
Cuts at: 954 954
Size: 3938
AclI AA'CG_TT
Cuts at: 3778 3778
Size: 3938
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 1763 2195 2942 3210 293
Size: 1470 432 747 268 1021
Fragments arranged by size:
1470 1021 747 432 268
AflIII A'CryG_T
Cuts at: 195 1290 2394 3371 195
Size: 1095 1104 977 762
Fragments arranged by size:
1104 1095 977 762
AgeI A'CCGG_T
Cuts at: 2326 2326
Size: 3938
AhdI GACnn_n'nnGTC
Cuts at: 696 696
Size: 3938
AleI CACnn'nnGTG
Cuts at: 475 796 475
Size: 321 3617
AlwNI CAG_nnn'CTG
Cuts at: 604 1243 2810 604
Size: 639 1567 1732
Fragments arranged by size:
1732 1567 639
ApaLI G'TGCA_C
Cuts at: 2708 2708
Size: 3938
ApoI r'AATT_y
Cuts at: 414 942 3291 3547 414
Size: 528 2349 256 805
Fragments arranged by size:
2349 805 528 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3938
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3938
AvaI C'yCGr_G
Cuts at: 238 958 238
Size: 720 3218
BamHI G'GATC_C
Cuts at: 963 963
Size: 3938
BanI G'GyrC_C
Cuts at: 513 880 954 1368 1616 1651 513
Size: 367 74 414 248 35 2800
Fragments arranged by size:
2800 414 367 248 74 35
BanII G_rGCy'C
Cuts at: 227 530 952 1982 227
Size: 303 422 1030 2183
Fragments arranged by size:
2183 1030 422 303
BbeI G_GCGC'C
Cuts at: 1372 1620 1372
Size: 248 3690
BciVI GTATCCnnnnn_n'
Cuts at: 1836 2597 3938 1836
Size: 761 1341 1836
Fragments arranged by size:
1836 1341 761
BclI T'GATC_A
Cuts at: 1458 1458
Size: 3938
BglI GCCn_nnn'nGGC
Cuts at: 444 444
Size: 3938
BglII A'GATC_T
Cuts at: 749 1453 3490 749
Size: 704 2037 1197
Fragments arranged by size:
2037 1197 704
BlpI GC'TnA_GC
Cuts at: 1050 1050
Size: 3938
Bme1580I G_kGCm'C
Cuts at: 518 883 1563 1656 2712 518
Size: 365 680 93 1056 1744
Fragments arranged by size:
1744 1056 680 365 93
BmrI ACTGGGnnnn_n'
Cuts at: 1150 1231 1566 3710 1150
Size: 81 335 2144 1378
Fragments arranged by size:
2144 1378 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2305 2305
Size: 3938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 307
Size: 3938
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1993 2485 2783 1114
Size: 879 492 298 2269
Fragments arranged by size:
2269 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 283 351 577 3642 283
Size: 68 226 3065 579
Fragments arranged by size:
3065 579 226 68
BsaAI yAC'GTr
Cuts at: 198 1291 198
Size: 1093 2845
BsaBI GATnn'nnATC
Cuts at: 6 781 1452 3489 6
Size: 775 671 2037 455
Fragments arranged by size:
2037 775 671 455
BsaHI Gr'CG_yC
Cuts at: 1369 1617 2319 3815 1369
Size: 248 702 1496 1492
Fragments arranged by size:
1496 1492 702 248
BsaWI w'CCGG_w
Cuts at: 1127 1648 2326 2600 2747 3158 1127
Size: 521 678 274 147 411 1907
Fragments arranged by size:
1907 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 921 921
Size: 3938
BseYI C'CCAG_C
Cuts at: 1363 2121 2698 3194 1363
Size: 758 577 496 2107
Fragments arranged by size:
2107 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 559 528
Size: 31 3907
BsiEI CG_ry'CG
Cuts at: 104 1480 1526 2734 104
Size: 1376 46 1208 1308
Fragments arranged by size:
1376 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 952 1730 1920 2712 952
Size: 778 190 792 2178
Fragments arranged by size:
2178 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 227 518 530 883 952 1563 1656 1730 1920
Size: 291 12 353 69 611 93 74 190
Cuts at: 1920 1982 2712 227
Size: 62 730 1453
Fragments arranged by size:
1453 730 611 353 291 190 93 74
69 62 12
BspEI T'CCGG_A
Cuts at: 3158 3158
Size: 3938
BspHI T'CATG_A
Cuts at: 3114 3114
Size: 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 397 438 988 1202 1504 1885 397
Size: 41 550 214 302 381 2450
Fragments arranged by size:
2450 550 381 302 214 41
BsrBI CCG'CTC
Cuts at: 50 898 2230 2284 3768 3933 50
Size: 848 1332 54 1484 165 55
Fragments arranged by size:
1484 1332 848 165 55 54
BsrDI GCAATG_nn'
Cuts at: 570 1850 570
Size: 1280 2658
BsrFI r'CCGG_y
Cuts at: 1936 2117 2326 1936
Size: 181 209 3548
Fragments arranged by size:
3548 209 181
BssSI C'ACGA_G
Cuts at: 2209 2567 2209
Size: 358 3580
BstAPI GCAn_nnn'nTGC
Cuts at: 604 1315 604
Size: 711 3227
BstBI TT'CG_AA
Cuts at: 2299 2299
Size: 3938
BstXI CCAn_nnnn'nTGG
Cuts at: 477 477
Size: 3938
BstYI r'GATC_y
Cuts at: 1 749 963 1430 1453 1788 2034 3046 3132
Size: 748 214 467 23 335 246 1012 86
Cuts at: 3132 3144 3490 1
Size: 12 346 449
Fragments arranged by size:
1012 748 467 449 346 335 246 214
86 23 12
BtgI C'CryG_G
Cuts at: 521 558 521
Size: 37 3901
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1888 2069 2232 1888
Size: 181 163 3594
Fragments arranged by size:
3594 181 163
BtsI GCAGTG_nn'
Cuts at: 460 1181 460
Size: 721 3217
DraI TTT'AAA
Cuts at: 937 937
Size: 3938
DrdI GACnn_nn'nnGTC
Cuts at: 1644 2502 1644
Size: 858 3080
EaeI y'GGCC_r
Cuts at: 657 852 928 1523 1697 2088 2115 3460 657
Size: 195 76 595 174 391 27 1345 1135
Fragments arranged by size:
1345 1135 595 391 195 174 76 27
EagI C'GGCC_G
Cuts at: 1523 1523
Size: 3938
EarI CTCTTCn'nnn_
Cuts at: 151 553 1961 2171 151
Size: 402 1408 210 1918
Fragments arranged by size:
1918 1408 402 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2146 2456 2602 3748 2146
Size: 310 146 1146 2336
Fragments arranged by size:
2336 1146 310 146
EcoICRI GAG'CTC
Cuts at: 950 950
Size: 3938
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 307 1763 2195 2942 3210 293
Size: 14 1456 432 747 268 1021
Fragments arranged by size:
1456 1021 747 432 268 14
EcoO109I rG'GnC_Cy
Cuts at: 128 233 1077 128
Size: 105 844 2989
Fragments arranged by size:
2989 844 105
EcoRI G'AATT_C
Cuts at: 414 942 414
Size: 528 3410
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1957 1957
Size: 3938
FspI TGC'GCA
Cuts at: 1719 1719
Size: 3938
HaeII r_GCGC'y
Cuts at: 1372 1620 2642 3621 1372
Size: 248 1022 979 1689
Fragments arranged by size:
1689 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 147 179 682 714 748 780 1780 1810 1812
Size: 32 503 32 34 32 1000 30 2
Cuts at: 1812 1842 147
Size: 30 2243
Fragments arranged by size:
2243 1000 503 34 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 246 977 246
Size: 731 3207
KasI G'GCGC_C
Cuts at: 1368 1616 1368
Size: 248 3690
KpnI G_GTAC'C
Cuts at: 958 958
Size: 3938
MluI A'CGCG_T
Cuts at: 3371 3371
Size: 3938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 443 725 746 2609 2793 443
Size: 282 21 1863 184 1588
Fragments arranged by size:
1863 1588 282 184 21
MscI TGG'CCA
Cuts at: 659 1699 659
Size: 1040 2898
MslI CAynn'nnrTG
Cuts at: 475 796 1927 2054 475
Size: 321 1131 127 2359
Fragments arranged by size:
2359 1131 321 127
MspA1I CmG'CkG
Cuts at: 873 1049 1363 1723 2736 2981 873
Size: 176 314 360 1013 245 1830
Fragments arranged by size:
1830 1013 360 314 245 176
NaeI GCC'GGC
Cuts at: 2119 2119
Size: 3938
NarI GG'CG_CC
Cuts at: 1369 1617 1369
Size: 248 3690
NgoMIV G'CCGG_C
Cuts at: 2117 2117
Size: 3938
NspI r_CATG'y
Cuts at: 991 1491 2398 991
Size: 500 907 2531
Fragments arranged by size:
2531 907 500
PciI A'CATG_T
Cuts at: 2394 2394
Size: 3938
PfoI T'CCnGG_A
Cuts at: 165 394 165
Size: 229 3709
PmeI GTTT'AAAC
Cuts at: 937 937
Size: 3938
PmlI CAC'GTG
Cuts at: 198 198
Size: 3938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3077 3109 3077
Size: 32 3906
PpuMI rG'GwC_Cy
Cuts at: 128 233 128
Size: 105 3833
PshAI GACnn'nnGTC
Cuts at: 829 829
Size: 3938
PspXI vC'TCGA_Gb
Cuts at: 238 238
Size: 3938
PstI C_TGCA'G
Cuts at: 253 985 1670 253
Size: 732 685 2521
Fragments arranged by size:
2521 732 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3938
PvuII CAG'CTG
Cuts at: 1363 1723 1363
Size: 360 3578
RsrII CG'GwC_CG
Cuts at: 2133 2133
Size: 3938
SacI G_AGCT'C
Cuts at: 952 952
Size: 3938
SalI G'TCGA_C
Cuts at: 975 975
Size: 3938
SanDI GG'GwC_CC
Cuts at: 233 233
Size: 3938
SapI GCTCTTCn'nnn_
Cuts at: 1961 2171 1961
Size: 210 3728
SbfI CC_TGCA'GG
Cuts at: 985 985
Size: 3938
ScaI AGT'ACT
Cuts at: 3455 3455
Size: 3938
SfcI C'TryA_G
Cuts at: 33 249 981 1666 2659 2850 33
Size: 216 732 685 993 191 1121
Fragments arranged by size:
1121 993 732 685 216 191
SfoI GGC'GCC
Cuts at: 1370 1618 1370
Size: 248 3690
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3938
SmaI CCC'GGG
Cuts at: 960 960
Size: 3938
SmlI C'TyrA_G
Cuts at: 238 1093 2008 2500 2762 238
Size: 855 915 492 262 1414
Fragments arranged by size:
1414 915 855 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3938
SphI G_CATG'C
Cuts at: 991 1491 991
Size: 500 3438
StuI AGG'CCT
Cuts at: 922 922
Size: 3938
StyI C'CwwG_G
Cuts at: 442 1072 1426 442
Size: 630 354 2954
Fragments arranged by size:
2954 630 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 268 1521 1654 2113 2322 268
Size: 1253 133 459 209 1884
Fragments arranged by size:
1884 1253 459 209 133
TatI w'GTAC_w
Cuts at: 3453 3453
Size: 3938
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3455 3487 3455
Size: 32 3906
Tth111I GACn'n_nGTC
Cuts at: 367 1735 367
Size: 1368 2570
XbaI T'CTAG_A
Cuts at: 297 969 297
Size: 672 3266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1433 1433
Size: 3938
XhoI C'TCGA_G
Cuts at: 238 238
Size: 3938
XmaI C'CCGG_G
Cuts at: 958 958
Size: 3938
XmnI GAAnn'nnTTC
Cuts at: 184 184
Size: 3938
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspXI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PsiI PspOMI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI ZraI