Restriction map for
pF1KB6385
(Circular) (Six-base) MAPSORT of: pF1KB6385.seq Check: 8222 from: 1 to: 3932
pF1KB6385 3932 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 533 970 533
Size: 437 3495
Acc65I G'GTAC_C
Cuts at: 491 948 491
Size: 457 3475
AclI AA'CG_TT
Cuts at: 3772 3772
Size: 3932
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 471 1757 2189 2936 3204 133
Size: 338 1286 432 747 268 861
Fragments arranged by size:
1286 861 747 432 338 268
AflIII A'CryG_T
Cuts at: 180 422 1284 2388 3365 180
Size: 242 862 1104 977 747
Fragments arranged by size:
1104 977 862 747 242
AgeI A'CCGG_T
Cuts at: 644 2320 644
Size: 1676 2256
AleI CACnn'nnGTG
Cuts at: 506 506
Size: 3932
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 170 202 170
Size: 32 3900
AlwNI CAG_nnn'CTG
Cuts at: 684 1237 2804 684
Size: 553 1567 1812
Fragments arranged by size:
1812 1567 553
ApaLI G'TGCA_C
Cuts at: 2702 2702
Size: 3932
ApoI r'AATT_y
Cuts at: 936 3285 3541 936
Size: 2349 256 1327
Fragments arranged by size:
2349 1327 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3932
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3932
AvaI C'yCGr_G
Cuts at: 131 189 952 131
Size: 58 763 3111
Fragments arranged by size:
3111 763 58
BamHI G'GATC_C
Cuts at: 957 957
Size: 3932
BanI G'GyrC_C
Cuts at: 268 491 769 948 1362 1610 1645 268
Size: 223 278 179 414 248 35 2555
Fragments arranged by size:
2555 414 278 248 223 179 35
BanII G_rGCy'C
Cuts at: 131 197 446 610 730 796 809 946 1976
Size: 66 249 164 120 66 13 137 1030
Cuts at: 1976 131
Size: 2087
Fragments arranged by size:
2087 1030 249 164 137 120 66 66
13
BbeI G_GCGC'C
Cuts at: 272 773 1366 1614 272
Size: 501 593 248 2590
Fragments arranged by size:
2590 593 501 248
BbsI GAAGACnn'nnnn_
Cuts at: 647 647
Size: 3932
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3898
BciVI GTATCCnnnnn_n'
Cuts at: 1830 2591 3932 1830
Size: 761 1341 1830
Fragments arranged by size:
1830 1341 761
BclI T'GATC_A
Cuts at: 755 1452 755
Size: 697 3235
BfrBI ATG'CAT
Cuts at: 169 169
Size: 3932
BglII A'GATC_T
Cuts at: 1447 3484 1447
Size: 2037 1895
BlpI GC'TnA_GC
Cuts at: 1044 1044
Size: 3932
Bme1580I G_kGCm'C
Cuts at: 1557 1650 2706 1557
Size: 93 1056 2783
Fragments arranged by size:
2783 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1144 1225 1560 3704 1144
Size: 81 335 2144 1372
Fragments arranged by size:
2144 1372 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2299 2299
Size: 3932
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 383 688 907 145
Size: 238 305 219 3170
Fragments arranged by size:
3170 305 238 219
Bpu10I CC'TnA_GC
Cuts at: 677 677
Size: 3932
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 1108 1987 2479 2777 181
Size: 927 879 492 298 1336
Fragments arranged by size:
1336 927 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3636 3636
Size: 3932
BsaAI yAC'GTr
Cuts at: 504 1285 504
Size: 781 3151
BsaBI GATnn'nnATC
Cuts at: 6 1446 3483 6
Size: 1440 2037 455
Fragments arranged by size:
2037 1440 455
BsaHI Gr'CG_yC
Cuts at: 269 377 403 770 1363 1611 2313 3809 269
Size: 108 26 367 593 248 702 1496 392
Fragments arranged by size:
1496 702 593 392 367 248 108 26
BsaWI w'CCGG_w
Cuts at: 644 1121 1642 2320 2594 2741 3152 644
Size: 477 521 678 274 147 411 1424
Fragments arranged by size:
1424 678 521 477 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 170 200 712 742 170
Size: 30 512 30 3360
Fragments arranged by size:
3360 512 30 30
BseYI C'CCAG_C
Cuts at: 1357 2115 2692 3188 1357
Size: 758 577 496 2101
Fragments arranged by size:
2101 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 928
Size: 3932
BsiEI CG_ry'CG
Cuts at: 104 299 1474 1520 2728 104
Size: 195 1175 46 1208 1308
Fragments arranged by size:
1308 1208 1175 195 46
BsiHKAI G_wGCw'C
Cuts at: 796 946 1724 1914 2706 796
Size: 150 778 190 792 2022
Fragments arranged by size:
2022 792 778 190 150
Bsp1286I G_dGCh'C
Cuts at: 131 197 446 610 730 796 809 946 1557
Size: 66 249 164 120 66 13 137 611
Cuts at: 1557 1650 1724 1914 1976 2706 131
Size: 93 74 190 62 730 1357
Fragments arranged by size:
1357 730 611 249 190 164 137 120
93 74 66 66 62 13
BspEI T'CCGG_A
Cuts at: 3152 3152
Size: 3932
BspHI T'CATG_A
Cuts at: 3108 3108
Size: 3932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 982 1196 1498 1879 982
Size: 214 302 381 3035
Fragments arranged by size:
3035 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2224 2278 3762 3927 50
Size: 2174 54 1484 165 55
Fragments arranged by size:
2174 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 744 1844 744
Size: 1100 2832
BsrFI r'CCGG_y
Cuts at: 644 1930 2111 2320 644
Size: 1286 181 209 2256
Fragments arranged by size:
2256 1286 209 181
BssSI C'ACGA_G
Cuts at: 2203 2561 2203
Size: 358 3574
BstAPI GCAn_nnn'nTGC
Cuts at: 1309 1309
Size: 3932
BstBI TT'CG_AA
Cuts at: 2293 2293
Size: 3932
BstEII G'GTnAC_C
Cuts at: 718 718
Size: 3932
BstXI CCAn_nnnn'nTGG
Cuts at: 396 396
Size: 3932
BstYI r'GATC_y
Cuts at: 1 323 710 957 1424 1447 1782 2028 3040
Size: 322 387 247 467 23 335 246 1012
Cuts at: 3040 3126 3138 3484 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 387 346 335 322 247
246 86 23 12
BtgI C'CryG_G
Cuts at: 371 455 570 371
Size: 84 115 3733
Fragments arranged by size:
3733 115 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 216 318 497 1882 2063 2226 216
Size: 102 179 1385 181 163 1922
Fragments arranged by size:
1922 1385 181 179 163 102
BtsI GCAGTG_nn'
Cuts at: 1175 1175
Size: 3932
DraI TTT'AAA
Cuts at: 931 931
Size: 3932
DraIII CAC_nnn'GTG
Cuts at: 837 837
Size: 3932
DrdI GACnn_nn'nnGTC
Cuts at: 1638 2496 1638
Size: 858 3074
EaeI y'GGCC_r
Cuts at: 452 822 1517 1691 2082 2109 3454 452
Size: 370 695 174 391 27 1345 930
Fragments arranged by size:
1345 930 695 391 370 174 27
EagI C'GGCC_G
Cuts at: 1517 1517
Size: 3932
EarI CTCTTCn'nnn_
Cuts at: 490 808 1955 2165 490
Size: 318 1147 210 2257
Fragments arranged by size:
2257 1147 318 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 319 2140 2450 2596 3742 319
Size: 1821 310 146 1146 509
Fragments arranged by size:
1821 1146 509 310 146
EcoICRI GAG'CTC
Cuts at: 794 944 794
Size: 150 3782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 145 383 471 688 907 1757 2189 2936
Size: 12 238 88 217 219 850 432 747
Cuts at: 2936 3204 133
Size: 268 861
Fragments arranged by size:
861 850 747 432 268 238 219 217
88 12
EcoO109I rG'GnC_Cy
Cuts at: 201 1071 201
Size: 870 3062
EcoRI G'AATT_C
Cuts at: 936 936
Size: 3932
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 345 1951 345
Size: 1606 2326
FspI TGC'GCA
Cuts at: 1713 1713
Size: 3932
HaeII r_GCGC'y
Cuts at: 272 773 1366 1614 2636 3615 272
Size: 501 593 248 1022 979 589
Fragments arranged by size:
1022 979 593 589 501 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 536 568 712 744 1774 1804 1806 1836 536
Size: 32 144 32 1030 30 2 30 2632
Fragments arranged by size:
2632 1030 144 32 32 30 30 2
HincII GTy'rAC
Cuts at: 971 971
Size: 3932
KasI G'GCGC_C
Cuts at: 268 769 1362 1610 268
Size: 501 593 248 2590
Fragments arranged by size:
2590 593 501 248
KpnI G_GTAC'C
Cuts at: 495 952 495
Size: 457 3475
MluI A'CGCG_T
Cuts at: 180 422 3365 180
Size: 242 2943 747
Fragments arranged by size:
2943 747 242
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2603 2787 2603
Size: 184 3748
MscI TGG'CCA
Cuts at: 454 1693 454
Size: 1239 2693
MslI CAynn'nnrTG
Cuts at: 394 506 569 1921 2048 394
Size: 112 63 1352 127 2278
Fragments arranged by size:
2278 1352 127 112 63
MspA1I CmG'CkG
Cuts at: 373 853 1043 1357 1717 2730 2975 373
Size: 480 190 314 360 1013 245 1330
Fragments arranged by size:
1330 1013 480 360 314 245 190
NaeI GCC'GGC
Cuts at: 2113 2113
Size: 3932
NarI GG'CG_CC
Cuts at: 269 770 1363 1611 269
Size: 501 593 248 2590
Fragments arranged by size:
2590 593 501 248
NcoI C'CATG_G
Cuts at: 570 570
Size: 3932
NgoMIV G'CCGG_C
Cuts at: 2111 2111
Size: 3932
NsiI A_TGCA'T
Cuts at: 171 171
Size: 3932
NspI r_CATG'y
Cuts at: 752 985 1485 2392 752
Size: 233 500 907 2292
Fragments arranged by size:
2292 907 500 233
PasI CC'CwG_GG
Cuts at: 603 603
Size: 3932
PciI A'CATG_T
Cuts at: 2388 2388
Size: 3932
PmeI GTTT'AAAC
Cuts at: 931 931
Size: 3932
PmlI CAC'GTG
Cuts at: 504 504
Size: 3932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 170 202 3071 3103 170
Size: 32 2869 32 999
Fragments arranged by size:
2869 999 32 32
PpuMI rG'GwC_Cy
Cuts at: 201 201
Size: 3932
PstI C_TGCA'G
Cuts at: 979 1664 979
Size: 685 3247
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3932
PvuII CAG'CTG
Cuts at: 853 1357 1717 853
Size: 504 360 3068
Fragments arranged by size:
3068 504 360
RsrII CG'GwC_CG
Cuts at: 2127 2127
Size: 3932
SacI G_AGCT'C
Cuts at: 796 946 796
Size: 150 3782
SacII CC_GC'GG
Cuts at: 374 374
Size: 3932
SalI G'TCGA_C
Cuts at: 969 969
Size: 3932
SapI GCTCTTCn'nnn_
Cuts at: 490 1955 2165 490
Size: 1465 210 2257
Fragments arranged by size:
2257 1465 210
SbfI CC_TGCA'GG
Cuts at: 979 979
Size: 3932
ScaI AGT'ACT
Cuts at: 3449 3449
Size: 3932
SfcI C'TryA_G
Cuts at: 33 579 975 1660 2653 2844 33
Size: 546 396 685 993 191 1121
Fragments arranged by size:
1121 993 685 546 396 191
SfoI GGC'GCC
Cuts at: 270 771 1364 1612 270
Size: 501 593 248 2590
Fragments arranged by size:
2590 593 501 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3932
SmaI CCC'GGG
Cuts at: 191 954 191
Size: 763 3169
SmlI C'TyrA_G
Cuts at: 196 1087 2002 2494 2756 196
Size: 891 915 492 262 1372
Fragments arranged by size:
1372 915 891 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3932
SphI G_CATG'C
Cuts at: 985 1485 985
Size: 500 3432
StyI C'CwwG_G
Cuts at: 570 1066 1420 570
Size: 496 354 3082
Fragments arranged by size:
3082 496 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 285 496 706 813 1515 1648 2107 2316 285
Size: 211 210 107 702 133 459 209 1901
Fragments arranged by size:
1901 702 459 211 210 209 133 107
TatI w'GTAC_w
Cuts at: 3447 3447
Size: 3932
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 702 734 3449 3481 702
Size: 32 2715 32 1153
Fragments arranged by size:
2715 1153 32 32
Tth111I GACn'n_nGTC
Cuts at: 256 1729 256
Size: 1473 2459
XbaI T'CTAG_A
Cuts at: 963 963
Size: 3932
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 237 396 838 1427 237
Size: 159 442 589 2742
Fragments arranged by size:
2742 589 442 159
XmaI C'CCGG_G
Cuts at: 189 952 189
Size: 763 3169
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BglI BmgBI BmtI BseRI BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PflMI PfoI PshAI PsiI
PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI