Restriction map for
pF1KB6380
(Circular) (Six-base) MAPSORT of: pF1KB6380.seq Check: 7026 from: 1 to: 4010
pF1KB6380 4010 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 1048 1048
Size: 4010
Acc65I G'GTAC_C
Cuts at: 1026 1026
Size: 4010
AclI AA'CG_TT
Cuts at: 3850 3850
Size: 4010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 710 1835 2267 3014 3282 545
Size: 165 1125 432 747 268 1273
Fragments arranged by size:
1273 1125 747 432 268 165
AflIII A'CryG_T
Cuts at: 1362 2466 3443 1362
Size: 1104 977 1929
Fragments arranged by size:
1929 1104 977
AgeI A'CCGG_T
Cuts at: 2398 2398
Size: 4010
AleI CACnn'nnGTG
Cuts at: 607 607
Size: 4010
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 878 910 878
Size: 32 3978
AlwNI CAG_nnn'CTG
Cuts at: 352 1315 2882 352
Size: 963 1567 1480
Fragments arranged by size:
1567 1480 963
ApaLI G'TGCA_C
Cuts at: 2780 2780
Size: 4010
ApoI r'AATT_y
Cuts at: 115 1014 3363 3619 115
Size: 899 2349 256 506
Fragments arranged by size:
2349 899 506 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4010
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
AvaI C'yCGr_G
Cuts at: 1030 1030
Size: 4010
BamHI G'GATC_C
Cuts at: 1035 1035
Size: 4010
BanI G'GyrC_C
Cuts at: 802 1026 1440 1688 1723 802
Size: 224 414 248 35 3089
Fragments arranged by size:
3089 414 248 224 35
BanII G_rGCy'C
Cuts at: 1024 2054 1024
Size: 1030 2980
BbeI G_GCGC'C
Cuts at: 1444 1692 1444
Size: 248 3762
BbsI GAAGACnn'nnnn_
Cuts at: 134 303 134
Size: 169 3841
BciVI GTATCCnnnnn_n'
Cuts at: 1908 2669 4010 1908
Size: 761 1341 1908
Fragments arranged by size:
1908 1341 761
BclI T'GATC_A
Cuts at: 1530 1530
Size: 4010
BglII A'GATC_T
Cuts at: 978 1525 3562 978
Size: 547 2037 1426
Fragments arranged by size:
2037 1426 547
BlpI GC'TnA_GC
Cuts at: 988 1122 988
Size: 134 3876
Bme1580I G_kGCm'C
Cuts at: 491 1635 1728 2784 491
Size: 1144 93 1056 1717
Fragments arranged by size:
1717 1144 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 858 1222 1303 1638 3782 858
Size: 364 81 335 2144 1086
Fragments arranged by size:
2144 1086 364 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 850 1186 2065 2557 2855 850
Size: 336 879 492 298 2005
Fragments arranged by size:
2005 879 492 336 298
BsaI GGTCTCn'nnnn_
Cuts at: 3714 3714
Size: 4010
BsaAI yAC'GTr
Cuts at: 162 1363 162
Size: 1201 2809
BsaBI GATnn'nnATC
Cuts at: 6 1524 3561 6
Size: 1518 2037 455
Fragments arranged by size:
2037 1518 455
BsaHI Gr'CG_yC
Cuts at: 1441 1689 2391 3887 1441
Size: 248 702 1496 1564
Fragments arranged by size:
1564 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1199 1720 2398 2672 2819 3230 1199
Size: 521 678 274 147 411 1979
Fragments arranged by size:
1979 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 346 385 532 160
Size: 186 39 147 3638
Fragments arranged by size:
3638 186 147 39
BseYI C'CCAG_C
Cuts at: 1435 2193 2770 3266 1435
Size: 758 577 496 2179
Fragments arranged by size:
2179 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 945 1552 1598 2806 104
Size: 841 607 46 1208 1308
Fragments arranged by size:
1308 1208 841 607 46
BsiHKAI G_wGCw'C
Cuts at: 826 1024 1802 1992 2784 826
Size: 198 778 190 792 2052
Fragments arranged by size:
2052 792 778 198 190
BsmI GAATG_Cn'
Cuts at: 278 278
Size: 4010
Bsp1286I G_dGCh'C
Cuts at: 491 826 1024 1635 1728 1802 1992 2054 2784
Size: 335 198 611 93 74 190 62 730
Cuts at: 2784 491
Size: 1717
Fragments arranged by size:
1717 730 611 335 198 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3230 3230
Size: 4010
BspHI T'CATG_A
Cuts at: 3186 3186
Size: 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1060 1274 1576 1957 1060
Size: 214 302 381 3113
Fragments arranged by size:
3113 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2302 2356 3840 4005 50
Size: 2252 54 1484 165 55
Fragments arranged by size:
2252 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1922 1922
Size: 4010
BsrFI r'CCGG_y
Cuts at: 2008 2189 2398 2008
Size: 181 209 3620
Fragments arranged by size:
3620 209 181
BssSI C'ACGA_G
Cuts at: 2281 2639 2281
Size: 358 3652
BstAPI GCAn_nnn'nTGC
Cuts at: 1387 1387
Size: 4010
BstBI TT'CG_AA
Cuts at: 2371 2371
Size: 4010
BstYI r'GATC_y
Cuts at: 1 978 1035 1502 1525 1860 2106 3118 3204
Size: 977 57 467 23 335 246 1012 86
Cuts at: 3204 3216 3562 1
Size: 12 346 449
Fragments arranged by size:
1012 977 467 449 346 335 246 86
57 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4010
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1960 2141 2304 1960
Size: 181 163 3666
Fragments arranged by size:
3666 181 163
BtsI GCAGTG_nn'
Cuts at: 1253 1253
Size: 4010
DraI TTT'AAA
Cuts at: 1009 1009
Size: 4010
DrdI GACnn_nn'nnGTC
Cuts at: 1716 2574 1716
Size: 858 3152
EaeI y'GGCC_r
Cuts at: 890 1595 1769 2160 2187 3532 890
Size: 705 174 391 27 1345 1368
Fragments arranged by size:
1368 1345 705 391 174 27
EagI C'GGCC_G
Cuts at: 1595 1595
Size: 4010
EarI CTCTTCn'nnn_
Cuts at: 227 286 317 323 521 557 863 2033 2243
Size: 59 31 6 198 36 306 1170 210
Cuts at: 2243 227
Size: 1994
Fragments arranged by size:
1994 1170 306 210 198 59 36 31
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2218 2528 2674 3820 2218
Size: 310 146 1146 2408
Fragments arranged by size:
2408 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1022 1022
Size: 4010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 710 1835 2267 3014 3282 545
Size: 165 1125 432 747 268 1273
Fragments arranged by size:
1273 1125 747 432 268 165
EcoO109I rG'GnC_Cy
Cuts at: 1149 1149
Size: 4010
EcoRI G'AATT_C
Cuts at: 115 1014 115
Size: 899 3111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 4010
FspI TGC'GCA
Cuts at: 1791 1791
Size: 4010
HaeII r_GCGC'y
Cuts at: 1444 1692 2714 3693 1444
Size: 248 1022 979 1761
Fragments arranged by size:
1761 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 646 661 678 693 1852 1882 1884 1914 646
Size: 15 17 15 1159 30 2 30 2742
Fragments arranged by size:
2742 1159 30 30 17 15 15 2
HincII GTy'rAC
Cuts at: 1049 1049
Size: 4010
KasI G'GCGC_C
Cuts at: 1440 1688 1440
Size: 248 3762
KpnI G_GTAC'C
Cuts at: 1030 1030
Size: 4010
MluI A'CGCG_T
Cuts at: 3443 3443
Size: 4010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 122 2681 2865 122
Size: 2559 184 1267
Fragments arranged by size:
2559 1267 184
MscI TGG'CCA
Cuts at: 892 1771 892
Size: 879 3131
MslI CAynn'nnrTG
Cuts at: 607 1999 2126 607
Size: 1392 127 2491
Fragments arranged by size:
2491 1392 127
MspA1I CmG'CkG
Cuts at: 1121 1435 1795 2808 3053 1121
Size: 314 360 1013 245 2078
Fragments arranged by size:
2078 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2191 2191
Size: 4010
NarI GG'CG_CC
Cuts at: 1441 1689 1441
Size: 248 3762
NcoI C'CATG_G
Cuts at: 107 107
Size: 4010
NgoMIV G'CCGG_C
Cuts at: 2189 2189
Size: 4010
NspI r_CATG'y
Cuts at: 1063 1563 2470 1063
Size: 500 907 2603
Fragments arranged by size:
2603 907 500
PciI A'CATG_T
Cuts at: 2466 2466
Size: 4010
PmeI GTTT'AAAC
Cuts at: 1009 1009
Size: 4010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3978
PstI C_TGCA'G
Cuts at: 390 1057 1742 390
Size: 667 685 2658
Fragments arranged by size:
2658 685 667
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4010
PvuII CAG'CTG
Cuts at: 1435 1795 1435
Size: 360 3650
RsrII CG'GwC_CG
Cuts at: 2205 2205
Size: 4010
SacI G_AGCT'C
Cuts at: 1024 1024
Size: 4010
SalI G'TCGA_C
Cuts at: 1047 1047
Size: 4010
SapI GCTCTTCn'nnn_
Cuts at: 227 557 2033 2243 227
Size: 330 1476 210 1994
Fragments arranged by size:
1994 1476 330 210
SbfI CC_TGCA'GG
Cuts at: 1057 1057
Size: 4010
ScaI AGT'ACT
Cuts at: 3527 3527
Size: 4010
SfcI C'TryA_G
Cuts at: 33 386 1053 1738 2731 2922 33
Size: 353 667 685 993 191 1121
Fragments arranged by size:
1121 993 685 667 353 191
SfoI GGC'GCC
Cuts at: 1442 1690 1442
Size: 248 3762
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
SmaI CCC'GGG
Cuts at: 1032 1032
Size: 4010
SmlI C'TyrA_G
Cuts at: 829 1165 2080 2572 2834 829
Size: 336 915 492 262 2005
Fragments arranged by size:
2005 915 492 336 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4010
SphI G_CATG'C
Cuts at: 1063 1563 1063
Size: 500 3510
StyI C'CwwG_G
Cuts at: 107 252 605 1144 1498 107
Size: 145 353 539 354 2619
Fragments arranged by size:
2619 539 354 353 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 959 1593 1726 2185 2394 959
Size: 634 133 459 209 2575
Fragments arranged by size:
2575 634 459 209 133
TatI w'GTAC_w
Cuts at: 496 3525 496
Size: 3029 981
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 786 818 3527 3559 786
Size: 32 2709 32 1237
Fragments arranged by size:
2709 1237 32 32
Tth111I GACn'n_nGTC
Cuts at: 1807 1807
Size: 4010
XbaI T'CTAG_A
Cuts at: 1041 1041
Size: 4010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1505 1505
Size: 4010
XmaI C'CCGG_G
Cuts at: 1030 1030
Size: 4010
XmnI GAAnn'nnTTC
Cuts at: 934 934
Size: 4010
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI