Restriction map for
pF1KB6375
(Circular) (Six-base) MAPSORT of: pF1KB6375.seq Check: 9136 from: 1 to: 3923
pF1KB6375 3923 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 305 305
Size: 3923
AccI GT'mk_AC
Cuts at: 961 961
Size: 3923
Acc65I G'GTAC_C
Cuts at: 939 939
Size: 3923
AclI AA'CG_TT
Cuts at: 3763 3763
Size: 3923
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1748 2180 2927 3195 1748
Size: 432 747 268 2476
Fragments arranged by size:
2476 747 432 268
AflIII A'CryG_T
Cuts at: 510 1275 2379 3356 510
Size: 765 1104 977 1077
Fragments arranged by size:
1104 1077 977 765
AgeI A'CCGG_T
Cuts at: 2311 2311
Size: 3923
AlwNI CAG_nnn'CTG
Cuts at: 1228 2795 1228
Size: 1567 2356
ApaI G_GGCC'C
Cuts at: 631 631
Size: 3923
ApaLI G'TGCA_C
Cuts at: 2693 2693
Size: 3923
ApoI r'AATT_y
Cuts at: 592 927 3276 3532 592
Size: 335 2349 256 983
Fragments arranged by size:
2349 983 335 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3923
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
AvaI C'yCGr_G
Cuts at: 943 943
Size: 3923
BamHI G'GATC_C
Cuts at: 948 948
Size: 3923
BanI G'GyrC_C
Cuts at: 939 1353 1601 1636 939
Size: 414 248 35 3226
Fragments arranged by size:
3226 414 248 35
BanII G_rGCy'C
Cuts at: 248 348 631 937 1967 248
Size: 100 283 306 1030 2204
Fragments arranged by size:
2204 1030 306 283 100
BbeI G_GCGC'C
Cuts at: 1357 1605 1357
Size: 248 3675
BbsI GAAGACnn'nnnn_
Cuts at: 428 587 428
Size: 159 3764
BbvCI CC'TCA_GC
Cuts at: 188 188
Size: 3923
BciVI GTATCCnnnnn_n'
Cuts at: 1821 2582 3923 1821
Size: 761 1341 1821
Fragments arranged by size:
1821 1341 761
BclI T'GATC_A
Cuts at: 1443 1443
Size: 3923
BglII A'GATC_T
Cuts at: 287 459 1438 3475 287
Size: 172 979 2037 735
Fragments arranged by size:
2037 979 735 172
BlpI GC'TnA_GC
Cuts at: 556 1035 556
Size: 479 3444
Bme1580I G_kGCm'C
Cuts at: 631 1548 1641 2697 631
Size: 917 93 1056 1857
Fragments arranged by size:
1857 1056 917 93
BmrI ACTGGGnnnn_n'
Cuts at: 886 1135 1216 1551 3695 886
Size: 249 81 335 2144 1114
Fragments arranged by size:
2144 1114 335 249 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 3923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 511
Size: 3923
Bpu10I CC'TnA_GC
Cuts at: 188 188
Size: 3923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 103 1099 1978 2470 2768 103
Size: 996 879 492 298 1258
Fragments arranged by size:
1258 996 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3627 3627
Size: 3923
BsaAI yAC'GTr
Cuts at: 513 1276 513
Size: 763 3160
BsaBI GATnn'nnATC
Cuts at: 6 158 1437 3474 6
Size: 152 1279 2037 455
Fragments arranged by size:
2037 1279 455 152
BsaHI Gr'CG_yC
Cuts at: 762 1354 1602 2304 3800 762
Size: 592 248 702 1496 885
Fragments arranged by size:
1496 885 702 592 248
BsaWI w'CCGG_w
Cuts at: 1112 1633 2311 2585 2732 3143 1112
Size: 521 678 274 147 411 1892
Fragments arranged by size:
1892 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 585 1348 2106 2683 3179 585
Size: 763 758 577 496 1329
Fragments arranged by size:
1329 763 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1465 1511 2719 104
Size: 1361 46 1208 1308
Fragments arranged by size:
1361 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 557 937 1715 1905 2697 557
Size: 380 778 190 792 1783
Fragments arranged by size:
1783 792 778 380 190
BsmI GAATG_Cn'
Cuts at: 332 378 332
Size: 46 3877
BsmBI CGTCTCn'nnnn_
Cuts at: 847 847
Size: 3923
Bsp1286I G_dGCh'C
Cuts at: 248 348 557 631 937 1548 1641 1715 1905
Size: 100 209 74 306 611 93 74 190
Cuts at: 1905 1967 2697 248
Size: 62 730 1474
Fragments arranged by size:
1474 730 611 306 209 190 100 93
74 74 62
BspEI T'CCGG_A
Cuts at: 3143 3143
Size: 3923
BspHI T'CATG_A
Cuts at: 3099 3099
Size: 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 305 973 1187 1489 1870 305
Size: 668 214 302 381 2358
Fragments arranged by size:
2358 668 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2215 2269 3753 3918 50
Size: 2165 54 1484 165 55
Fragments arranged by size:
2165 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 533 1835 533
Size: 1302 2621
BsrFI r'CCGG_y
Cuts at: 1921 2102 2311 1921
Size: 181 209 3533
Fragments arranged by size:
3533 209 181
BssSI C'ACGA_G
Cuts at: 358 2194 2552 358
Size: 1836 358 1729
Fragments arranged by size:
1836 1729 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1300 1300
Size: 3923
BstBI TT'CG_AA
Cuts at: 324 837 2284 324
Size: 513 1447 1963
Fragments arranged by size:
1963 1447 513
BstEII G'GTnAC_C
Cuts at: 693 693
Size: 3923
BstYI r'GATC_y
Cuts at: 1 287 459 948 1415 1438 1773 2019 3031
Size: 286 172 489 467 23 335 246 1012
Cuts at: 3031 3117 3129 3475 1
Size: 86 12 346 449
Fragments arranged by size:
1012 489 467 449 346 335 286 246
172 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 497 497
Size: 3923
BtgI C'CryG_G
Cuts at: 107 824 107
Size: 717 3206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1873 2054 2217 1873
Size: 181 163 3579
Fragments arranged by size:
3579 181 163
BtsI GCAGTG_nn'
Cuts at: 1166 1166
Size: 3923
DraI TTT'AAA
Cuts at: 597 922 597
Size: 325 3598
DrdI GACnn_nn'nnGTC
Cuts at: 1629 2487 1629
Size: 858 3065
EaeI y'GGCC_r
Cuts at: 1508 1682 2073 2100 3445 1508
Size: 174 391 27 1345 1986
Fragments arranged by size:
1986 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1508 1508
Size: 3923
EarI CTCTTCn'nnn_
Cuts at: 845 853 1946 2156 845
Size: 8 1093 210 2612
Fragments arranged by size:
2612 1093 210 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2131 2441 2587 3733 2131
Size: 310 146 1146 2321
Fragments arranged by size:
2321 1146 310 146
EcoICRI GAG'CTC
Cuts at: 935 935
Size: 3923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 1748 2180 2927 3195 511
Size: 1237 432 747 268 1239
Fragments arranged by size:
1239 1237 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 231 619 628 717 888 1062 231
Size: 388 9 89 171 174 3092
Fragments arranged by size:
3092 388 174 171 89 9
EcoRI G'AATT_C
Cuts at: 927 927
Size: 3923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1942 1942
Size: 3923
FspI TGC'GCA
Cuts at: 1704 1704
Size: 3923
HaeII r_GCGC'y
Cuts at: 1357 1605 2627 3606 1357
Size: 248 1022 979 1674
Fragments arranged by size:
1674 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1765 1795 1797 1827 1765
Size: 30 2 30 3861
Fragments arranged by size:
3861 30 30 2
HincII GTy'rAC
Cuts at: 816 962 816
Size: 146 3777
KasI G'GCGC_C
Cuts at: 1353 1601 1353
Size: 248 3675
KpnI G_GTAC'C
Cuts at: 943 943
Size: 3923
MfeI C'AATT_G
Cuts at: 633 633
Size: 3923
MluI A'CGCG_T
Cuts at: 3356 3356
Size: 3923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2594 2778 2594
Size: 184 3739
MscI TGG'CCA
Cuts at: 1684 1684
Size: 3923
MslI CAynn'nnrTG
Cuts at: 675 1912 2039 675
Size: 1237 127 2559
Fragments arranged by size:
2559 1237 127
MspA1I CmG'CkG
Cuts at: 174 789 867 1034 1348 1708 2721 2966 174
Size: 615 78 167 314 360 1013 245 1131
Fragments arranged by size:
1131 1013 615 360 314 245 167 78
NaeI GCC'GGC
Cuts at: 2104 2104
Size: 3923
NarI GG'CG_CC
Cuts at: 1354 1602 1354
Size: 248 3675
NcoI C'CATG_G
Cuts at: 107 824 107
Size: 717 3206
NgoMIV G'CCGG_C
Cuts at: 2102 2102
Size: 3923
NspI r_CATG'y
Cuts at: 976 1476 2383 976
Size: 500 907 2516
Fragments arranged by size:
2516 907 500
PciI A'CATG_T
Cuts at: 2379 2379
Size: 3923
PflMI CCAn_nnn'nTGG
Cuts at: 463 463
Size: 3923
PmeI GTTT'AAAC
Cuts at: 922 922
Size: 3923
PmlI CAC'GTG
Cuts at: 513 513
Size: 3923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3062 3094 3062
Size: 32 3891
PpuMI rG'GwC_Cy
Cuts at: 231 619 888 231
Size: 388 269 3266
Fragments arranged by size:
3266 388 269
PspOMI G'GGCC_C
Cuts at: 627 627
Size: 3923
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 384 416 384
Size: 32 3891
PstI C_TGCA'G
Cuts at: 302 391 725 970 1655 302
Size: 89 334 245 685 2570
Fragments arranged by size:
2570 685 334 245 89
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3923
PvuII CAG'CTG
Cuts at: 174 789 867 1348 1708 174
Size: 615 78 481 360 2389
Fragments arranged by size:
2389 615 481 360 78
RsrII CG'GwC_CG
Cuts at: 2118 2118
Size: 3923
SacI G_AGCT'C
Cuts at: 937 937
Size: 3923
SalI G'TCGA_C
Cuts at: 960 960
Size: 3923
SapI GCTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
SbfI CC_TGCA'GG
Cuts at: 970 970
Size: 3923
ScaI AGT'ACT
Cuts at: 3440 3440
Size: 3923
SfcI C'TryA_G
Cuts at: 33 149 298 387 721 966 1651 2644 2835
Size: 116 149 89 334 245 685 993 191
Cuts at: 2835 33
Size: 1121
Fragments arranged by size:
1121 993 685 334 245 191 149 116
89
SfoI GGC'GCC
Cuts at: 1355 1603 1355
Size: 248 3675
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
SmaI CCC'GGG
Cuts at: 945 945
Size: 3923
SmlI C'TyrA_G
Cuts at: 118 1078 1993 2485 2747 118
Size: 960 915 492 262 1294
Fragments arranged by size:
1294 960 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3923
SphI G_CATG'C
Cuts at: 976 1476 976
Size: 500 3423
StyI C'CwwG_G
Cuts at: 107 623 824 1057 1411 107
Size: 516 201 233 354 2619
Fragments arranged by size:
2619 516 354 233 201
SwaI ATTT'AAAT
Cuts at: 597 597
Size: 3923
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1506 1639 2098 2307 1506
Size: 133 459 209 3122
Fragments arranged by size:
3122 459 209 133
TatI w'GTAC_w
Cuts at: 275 410 3438 275
Size: 135 3028 760
Fragments arranged by size:
3028 760 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 3891
Tth111I GACn'n_nGTC
Cuts at: 1720 1720
Size: 3923
XbaI T'CTAG_A
Cuts at: 954 954
Size: 3923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1418 1418
Size: 3923
XmaI C'CCGG_G
Cuts at: 943 943
Size: 3923
XmnI GAAnn'nnTTC
Cuts at: 425 425
Size: 3923
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PmlI
PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BseRI BsgI BsiWI BsrGI BssHII BstXI BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PshAI PsiI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI XhoI ZraI