Restriction map for
pF1KB6374
(Circular) (Six-base) MAPSORT of: pF1KB6374.seq Check: 9923 from: 1 to: 3941
pF1KB6374 3941 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 979 979
Size: 3941
Acc65I G'GTAC_C
Cuts at: 957 957
Size: 3941
AclI AA'CG_TT
Cuts at: 3781 3781
Size: 3941
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 371 394 1766 2198 2945 3213 156
Size: 215 23 1372 432 747 268 884
Fragments arranged by size:
1372 884 747 432 268 215 23
AflIII A'CryG_T
Cuts at: 128 1293 2397 3374 128
Size: 1165 1104 977 695
Fragments arranged by size:
1165 1104 977 695
AgeI A'CCGG_T
Cuts at: 2329 2329
Size: 3941
AlwNI CAG_nnn'CTG
Cuts at: 1246 2813 1246
Size: 1567 2374
ApaI G_GGCC'C
Cuts at: 149 158 149
Size: 9 3932
ApaLI G'TGCA_C
Cuts at: 790 2711 790
Size: 1921 2020
ApoI r'AATT_y
Cuts at: 240 945 3294 3550 240
Size: 705 2349 256 631
Fragments arranged by size:
2349 705 631 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3941
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3941
AvaI C'yCGr_G
Cuts at: 141 150 961 141
Size: 9 811 3121
Fragments arranged by size:
3121 811 9
BamHI G'GATC_C
Cuts at: 966 966
Size: 3941
BanI G'GyrC_C
Cuts at: 957 1371 1619 1654 957
Size: 414 248 35 3244
Fragments arranged by size:
3244 414 248 35
BanII G_rGCy'C
Cuts at: 149 158 955 1985 149
Size: 9 797 1030 2105
Fragments arranged by size:
2105 1030 797 9
BbeI G_GCGC'C
Cuts at: 1375 1623 1375
Size: 248 3693
BciVI GTATCCnnnnn_n'
Cuts at: 874 1839 2600 3941 874
Size: 965 761 1341 874
Fragments arranged by size:
1341 965 874 761
BclI T'GATC_A
Cuts at: 1461 1461
Size: 3941
BglI GCCn_nnn'nGGC
Cuts at: 153 153
Size: 3941
BglII A'GATC_T
Cuts at: 1456 3493 1456
Size: 2037 1904
BlpI GC'TnA_GC
Cuts at: 1053 1053
Size: 3941
Bme1580I G_kGCm'C
Cuts at: 149 158 794 1566 1659 2715 149
Size: 9 636 772 93 1056 1375
Fragments arranged by size:
1375 1056 772 636 93 9
BmgBI CAC'GTC
Cuts at: 191 795 191
Size: 604 3337
BmrI ACTGGGnnnn_n'
Cuts at: 1153 1234 1569 3713 1153
Size: 81 335 2144 1381
Fragments arranged by size:
2144 1381 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 3941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 700
Size: 3941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 1117 1996 2488 2786 258
Size: 859 879 492 298 1413
Fragments arranged by size:
1413 879 859 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 570 3645 570
Size: 3075 866
BsaAI yAC'GTr
Cuts at: 1294 1294
Size: 3941
BsaBI GATnn'nnATC
Cuts at: 6 701 862 1455 3492 6
Size: 695 161 593 2037 455
Fragments arranged by size:
2037 695 593 455 161
BsaHI Gr'CG_yC
Cuts at: 1372 1620 2322 3818 1372
Size: 248 702 1496 1495
Fragments arranged by size:
1496 1495 702 248
BsaWI w'CCGG_w
Cuts at: 134 1130 1651 2329 2603 2750 3161 134
Size: 996 521 678 274 147 411 914
Fragments arranged by size:
996 914 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 568 598 568
Size: 30 3911
BseYI C'CCAG_C
Cuts at: 158 1366 2124 2701 3197 158
Size: 1208 758 577 496 902
Fragments arranged by size:
1208 902 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 487 213
Size: 274 3667
BsiEI CG_ry'CG
Cuts at: 104 1483 1529 2737 104
Size: 1379 46 1208 1308
Fragments arranged by size:
1379 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 794 955 1733 1923 2715 794
Size: 161 778 190 792 2020
Fragments arranged by size:
2020 792 778 190 161
Bsp1286I G_dGCh'C
Cuts at: 149 158 794 955 1566 1659 1733 1923 1985
Size: 9 636 161 611 93 74 190 62
Cuts at: 1985 2715 149
Size: 730 1375
Fragments arranged by size:
1375 730 636 611 190 161 93 74
62 9
BspEI T'CCGG_A
Cuts at: 3161 3161
Size: 3941
BspHI T'CATG_A
Cuts at: 425 3117 425
Size: 2692 1249
BspMI ACCTGCnnnn'nnnn_
Cuts at: 991 1205 1507 1888 991
Size: 214 302 381 3044
Fragments arranged by size:
3044 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2233 2287 3771 3936 50
Size: 2183 54 1484 165 55
Fragments arranged by size:
2183 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1853 1853
Size: 3941
BsrFI r'CCGG_y
Cuts at: 1939 2120 2329 1939
Size: 181 209 3551
Fragments arranged by size:
3551 209 181
BssSI C'ACGA_G
Cuts at: 2212 2570 2212
Size: 358 3583
BstAPI GCAn_nnn'nTGC
Cuts at: 1318 1318
Size: 3941
BstBI TT'CG_AA
Cuts at: 2302 2302
Size: 3941
BstYI r'GATC_y
Cuts at: 1 520 966 1433 1456 1791 2037 3049 3135
Size: 519 446 467 23 335 246 1012 86
Cuts at: 3135 3147 3493 1
Size: 12 346 449
Fragments arranged by size:
1012 519 467 449 446 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1891 2072 2235 1891
Size: 181 163 3597
Fragments arranged by size:
3597 181 163
BtsI GCAGTG_nn'
Cuts at: 224 1184 224
Size: 960 2981
DraI TTT'AAA
Cuts at: 714 940 714
Size: 226 3715
DrdI GACnn_nn'nnGTC
Cuts at: 1647 2505 1647
Size: 858 3083
EaeI y'GGCC_r
Cuts at: 620 722 1526 1700 2091 2118 3463 620
Size: 102 804 174 391 27 1345 1098
Fragments arranged by size:
1345 1098 804 391 174 102 27
EagI C'GGCC_G
Cuts at: 1526 1526
Size: 3941
EarI CTCTTCn'nnn_
Cuts at: 533 1964 2174 533
Size: 1431 210 2300
Fragments arranged by size:
2300 1431 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2149 2459 2605 3751 2149
Size: 310 146 1146 2339
Fragments arranged by size:
2339 1146 310 146
EcoICRI GAG'CTC
Cuts at: 953 953
Size: 3941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 371 394 700 1766 2198 2945 3213 156
Size: 215 23 306 1066 432 747 268 884
Fragments arranged by size:
1066 884 747 432 306 268 215 23
EcoO109I rG'GnC_Cy
Cuts at: 145 146 154 155 362 1080 145
Size: 1 8 1 207 718 3006
Fragments arranged by size:
3006 718 207 8 1 1
EcoRI G'AATT_C
Cuts at: 945 945
Size: 3941
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1960 1960
Size: 3941
FspI TGC'GCA
Cuts at: 1722 1722
Size: 3941
HaeII r_GCGC'y
Cuts at: 399 1375 1623 2645 3624 399
Size: 976 248 1022 979 716
Fragments arranged by size:
1022 979 976 716 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 896 928 1783 1813 1815 1845 896
Size: 32 855 30 2 30 2992
Fragments arranged by size:
2992 855 32 30 30 2
HincII GTy'rAC
Cuts at: 980 980
Size: 3941
KasI G'GCGC_C
Cuts at: 1371 1619 1371
Size: 248 3693
KpnI G_GTAC'C
Cuts at: 961 961
Size: 3941
MluI A'CGCG_T
Cuts at: 3374 3374
Size: 3941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2612 2796 2612
Size: 184 3757
MscI TGG'CCA
Cuts at: 622 724 1702 622
Size: 102 978 2861
Fragments arranged by size:
2861 978 102
MslI CAynn'nnrTG
Cuts at: 1930 2057 1930
Size: 127 3814
MspA1I CmG'CkG
Cuts at: 197 210 561 678 1052 1366 1726 2739 2984
Size: 13 351 117 374 314 360 1013 245
Cuts at: 2984 197
Size: 1154
Fragments arranged by size:
1154 1013 374 360 351 314 245 117
13
NaeI GCC'GGC
Cuts at: 2122 2122
Size: 3941
NarI GG'CG_CC
Cuts at: 1372 1620 1372
Size: 248 3693
NgoMIV G'CCGG_C
Cuts at: 2120 2120
Size: 3941
NspI r_CATG'y
Cuts at: 132 994 1494 2401 132
Size: 862 500 907 1672
Fragments arranged by size:
1672 907 862 500
PciI A'CATG_T
Cuts at: 128 2397 128
Size: 2269 1672
PfoI T'CCnGG_A
Cuts at: 164 164
Size: 3941
PmeI GTTT'AAAC
Cuts at: 940 940
Size: 3941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3080 3112 3080
Size: 32 3909
PpuMI rG'GwC_Cy
Cuts at: 362 362
Size: 3941
PsiI TTA'TAA
Cuts at: 926 926
Size: 3941
PspOMI G'GGCC_C
Cuts at: 145 154 145
Size: 9 3932
PstI C_TGCA'G
Cuts at: 444 537 988 1673 444
Size: 93 451 685 2712
Fragments arranged by size:
2712 685 451 93
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3941
PvuII CAG'CTG
Cuts at: 561 678 1366 1726 561
Size: 117 688 360 2776
Fragments arranged by size:
2776 688 360 117
RsrII CG'GwC_CG
Cuts at: 2136 2136
Size: 3941
SacI G_AGCT'C
Cuts at: 955 955
Size: 3941
SalI G'TCGA_C
Cuts at: 978 978
Size: 3941
SapI GCTCTTCn'nnn_
Cuts at: 1964 2174 1964
Size: 210 3731
SbfI CC_TGCA'GG
Cuts at: 988 988
Size: 3941
ScaI AGT'ACT
Cuts at: 3458 3458
Size: 3941
SfcI C'TryA_G
Cuts at: 33 283 440 533 984 1669 2662 2853 33
Size: 250 157 93 451 685 993 191 1121
Fragments arranged by size:
1121 993 685 451 250 191 157 93
SfiI GGCCn_nnn'nGGCC
Cuts at: 153 153
Size: 3941
SfoI GGC'GCC
Cuts at: 1373 1621 1373
Size: 248 3693
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3941
SmaI CCC'GGG
Cuts at: 143 963 143
Size: 820 3121
SmlI C'TyrA_G
Cuts at: 273 1096 2011 2503 2765 273
Size: 823 915 492 262 1449
Fragments arranged by size:
1449 915 823 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3941
SphI G_CATG'C
Cuts at: 994 1494 994
Size: 500 3441
StyI C'CwwG_G
Cuts at: 329 1075 1429 329
Size: 746 354 2841
Fragments arranged by size:
2841 746 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1524 1657 2116 2325 1524
Size: 133 459 209 3140
Fragments arranged by size:
3140 459 209 133
TatI w'GTAC_w
Cuts at: 3456 3456
Size: 3941
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3458 3490 3458
Size: 32 3909
Tth111I GACn'n_nGTC
Cuts at: 1738 1738
Size: 3941
XbaI T'CTAG_A
Cuts at: 972 972
Size: 3941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1436 1436
Size: 3941
XmaI C'CCGG_G
Cuts at: 141 961 141
Size: 820 3121
XmnI GAAnn'nnTTC
Cuts at: 433 433
Size: 3941
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI