Restriction map for
pF1KB6365
(Circular) (Six-base) MAPSORT of: pF1KB6365.seq Check: 4925 from: 1 to: 3917
pF1KB6365 3917 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 955 955
Size: 3917
Acc65I G'GTAC_C
Cuts at: 933 933
Size: 3917
AclI AA'CG_TT
Cuts at: 3757 3757
Size: 3917
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 1742 2174 2921 3189 123
Size: 1619 432 747 268 851
Fragments arranged by size:
1619 851 747 432 268
AfeI AGC'GCT
Cuts at: 264 264
Size: 3917
AflIII A'CryG_T
Cuts at: 1269 2373 3350 1269
Size: 1104 977 1836
Fragments arranged by size:
1836 1104 977
AgeI A'CCGG_T
Cuts at: 2305 2305
Size: 3917
AlwNI CAG_nnn'CTG
Cuts at: 161 1222 2789 161
Size: 1061 1567 1289
Fragments arranged by size:
1567 1289 1061
ApaLI G'TGCA_C
Cuts at: 2687 2687
Size: 3917
ApoI r'AATT_y
Cuts at: 526 921 3270 3526 526
Size: 395 2349 256 917
Fragments arranged by size:
2349 917 395 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3917
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3917
AvaI C'yCGr_G
Cuts at: 357 719 937 357
Size: 362 218 3337
Fragments arranged by size:
3337 362 218
BamHI G'GATC_C
Cuts at: 439 942 439
Size: 503 3414
BanI G'GyrC_C
Cuts at: 362 933 1347 1595 1630 362
Size: 571 414 248 35 2649
Fragments arranged by size:
2649 571 414 248 35
BanII G_rGCy'C
Cuts at: 168 355 931 1961 168
Size: 187 576 1030 2124
Fragments arranged by size:
2124 1030 576 187
BbeI G_GCGC'C
Cuts at: 366 1351 1599 366
Size: 985 248 2684
Fragments arranged by size:
2684 985 248
BbsI GAAGACnn'nnnn_
Cuts at: 263 263
Size: 3917
BbvCI CC'TCA_GC
Cuts at: 154 633 154
Size: 479 3438
BciVI GTATCCnnnnn_n'
Cuts at: 1815 2576 3917 1815
Size: 761 1341 1815
Fragments arranged by size:
1815 1341 761
BclI T'GATC_A
Cuts at: 1437 1437
Size: 3917
BglI GCCn_nnn'nGGC
Cuts at: 371 371
Size: 3917
BglII A'GATC_T
Cuts at: 413 1432 3469 413
Size: 1019 2037 861
Fragments arranged by size:
2037 1019 861
BlpI GC'TnA_GC
Cuts at: 1029 1029
Size: 3917
Bme1580I G_kGCm'C
Cuts at: 1542 1635 2691 1542
Size: 93 1056 2768
Fragments arranged by size:
2768 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1129 1210 1545 3689 1129
Size: 81 335 2144 1357
Fragments arranged by size:
2144 1357 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2284 2284
Size: 3917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 222
Size: 3917
Bpu10I CC'TnA_GC
Cuts at: 154 633 154
Size: 479 3438
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1093 1972 2464 2762 880
Size: 213 879 492 298 2035
Fragments arranged by size:
2035 879 492 298 213
BsaI GGTCTCn'nnnn_
Cuts at: 3621 3621
Size: 3917
BsaAI yAC'GTr
Cuts at: 1270 1270
Size: 3917
BsaBI GATnn'nnATC
Cuts at: 6 1431 3468 6
Size: 1425 2037 455
Fragments arranged by size:
2037 1425 455
BsaHI Gr'CG_yC
Cuts at: 363 1348 1596 2298 3794 363
Size: 985 248 702 1496 486
Fragments arranged by size:
1496 985 702 486 248
BsaWI w'CCGG_w
Cuts at: 1106 1627 2305 2579 2726 3137 1106
Size: 521 678 274 147 411 1886
Fragments arranged by size:
1886 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 115 118 121 276 279 524 115
Size: 3 3 155 3 245 3508
Fragments arranged by size:
3508 245 155 3 3 3
BseYI C'CCAG_C
Cuts at: 731 1342 2100 2677 3173 731
Size: 611 758 577 496 1475
Fragments arranged by size:
1475 758 611 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 732 842 732
Size: 110 3807
BsiEI CG_ry'CG
Cuts at: 104 1459 1505 2713 104
Size: 1355 46 1208 1308
Fragments arranged by size:
1355 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 931 1709 1899 2691 931
Size: 778 190 792 2157
Fragments arranged by size:
2157 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 187 187
Size: 3917
Bsp1286I G_dGCh'C
Cuts at: 168 355 931 1542 1635 1709 1899 1961 2691
Size: 187 576 611 93 74 190 62 730
Cuts at: 2691 168
Size: 1394
Fragments arranged by size:
1394 730 611 576 190 187 93 74
62
BspEI T'CCGG_A
Cuts at: 3137 3137
Size: 3917
BspHI T'CATG_A
Cuts at: 3093 3093
Size: 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 967 1181 1483 1864 967
Size: 214 302 381 3020
Fragments arranged by size:
3020 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2209 2263 3747 3912 50
Size: 2159 54 1484 165 55
Fragments arranged by size:
2159 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1829 1829
Size: 3917
BsrFI r'CCGG_y
Cuts at: 370 1915 2096 2305 370
Size: 1545 181 209 1982
Fragments arranged by size:
1982 1545 209 181
BssSI C'ACGA_G
Cuts at: 2188 2546 2188
Size: 358 3559
BstAPI GCAn_nnn'nTGC
Cuts at: 1294 1294
Size: 3917
BstBI TT'CG_AA
Cuts at: 2278 2278
Size: 3917
BstXI CCAn_nnnn'nTGG
Cuts at: 322 322
Size: 3917
BstYI r'GATC_y
Cuts at: 1 413 439 942 1409 1432 1767 2013 3025
Size: 412 26 503 467 23 335 246 1012
Cuts at: 3025 3111 3123 3469 1
Size: 86 12 346 449
Fragments arranged by size:
1012 503 467 449 412 346 335 246
86 26 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 550 1867 2048 2211 550
Size: 1317 181 163 2256
Fragments arranged by size:
2256 1317 181 163
BtsI GCAGTG_nn'
Cuts at: 1160 1160
Size: 3917
DraI TTT'AAA
Cuts at: 916 916
Size: 3917
DrdI GACnn_nn'nnGTC
Cuts at: 1623 2481 1623
Size: 858 3059
EaeI y'GGCC_r
Cuts at: 1502 1676 2067 2094 3439 1502
Size: 174 391 27 1345 1980
Fragments arranged by size:
1980 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1502 1502
Size: 3917
EarI CTCTTCn'nnn_
Cuts at: 139 282 1940 2150 139
Size: 143 1658 210 1906
Fragments arranged by size:
1906 1658 210 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2125 2435 2581 3727 2125
Size: 310 146 1146 2315
Fragments arranged by size:
2315 1146 310 146
EcoICRI GAG'CTC
Cuts at: 929 929
Size: 3917
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 222 1742 2174 2921 3189 123
Size: 99 1520 432 747 268 851
Fragments arranged by size:
1520 851 747 432 268 99
EcoO109I rG'GnC_Cy
Cuts at: 1056 1056
Size: 3917
EcoRI G'AATT_C
Cuts at: 921 921
Size: 3917
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 921 1936 921
Size: 1015 2902
FspI TGC'GCA
Cuts at: 1698 1698
Size: 3917
HaeII r_GCGC'y
Cuts at: 161 266 366 1351 1599 2621 3600 161
Size: 105 100 985 248 1022 979 478
Fragments arranged by size:
1022 985 979 478 248 105 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 380 412 1759 1789 1791 1821 380
Size: 32 1347 30 2 30 2476
Fragments arranged by size:
2476 1347 32 30 30 2
HincII GTy'rAC
Cuts at: 956 956
Size: 3917
KasI G'GCGC_C
Cuts at: 362 1347 1595 362
Size: 985 248 2684
Fragments arranged by size:
2684 985 248
KpnI G_GTAC'C
Cuts at: 937 937
Size: 3917
MluI A'CGCG_T
Cuts at: 3350 3350
Size: 3917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 752 2588 2772 752
Size: 1836 184 1897
Fragments arranged by size:
1897 1836 184
MscI TGG'CCA
Cuts at: 1678 1678
Size: 3917
MslI CAynn'nnrTG
Cuts at: 563 1906 2033 563
Size: 1343 127 2447
Fragments arranged by size:
2447 1343 127
MspA1I CmG'CkG
Cuts at: 211 1028 1342 1702 2715 2960 211
Size: 817 314 360 1013 245 1168
Fragments arranged by size:
1168 1013 817 360 314 245
NaeI GCC'GGC
Cuts at: 2098 2098
Size: 3917
NarI GG'CG_CC
Cuts at: 363 1348 1596 363
Size: 985 248 2684
Fragments arranged by size:
2684 985 248
NgoMIV G'CCGG_C
Cuts at: 2096 2096
Size: 3917
NspI r_CATG'y
Cuts at: 970 1470 2377 970
Size: 500 907 2510
Fragments arranged by size:
2510 907 500
PciI A'CATG_T
Cuts at: 2373 2373
Size: 3917
PmeI GTTT'AAAC
Cuts at: 916 916
Size: 3917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3056 3088 3056
Size: 32 3885
PstI C_TGCA'G
Cuts at: 388 413 964 1649 388
Size: 25 551 685 2656
Fragments arranged by size:
2656 685 551 25
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3917
PvuII CAG'CTG
Cuts at: 1342 1702 1342
Size: 360 3557
RsrII CG'GwC_CG
Cuts at: 368 2112 368
Size: 1744 2173
SacI G_AGCT'C
Cuts at: 931 931
Size: 3917
SalI G'TCGA_C
Cuts at: 954 954
Size: 3917
SapI GCTCTTCn'nnn_
Cuts at: 1940 2150 1940
Size: 210 3707
SbfI CC_TGCA'GG
Cuts at: 964 964
Size: 3917
ScaI AGT'ACT
Cuts at: 3434 3434
Size: 3917
SfcI C'TryA_G
Cuts at: 33 384 409 960 1645 2638 2829 33
Size: 351 25 551 685 993 191 1121
Fragments arranged by size:
1121 993 685 551 351 191 25
SfoI GGC'GCC
Cuts at: 364 1349 1597 364
Size: 985 248 2684
Fragments arranged by size:
2684 985 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3917
SmaI CCC'GGG
Cuts at: 721 939 721
Size: 218 3699
SmlI C'TyrA_G
Cuts at: 895 1072 1987 2479 2741 895
Size: 177 915 492 262 2071
Fragments arranged by size:
2071 915 492 262 177
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3917
SphI G_CATG'C
Cuts at: 970 1470 970
Size: 500 3417
SspI AAT'ATT
Cuts at: 476 476
Size: 3917
StyI C'CwwG_G
Cuts at: 315 1051 1405 315
Size: 736 354 2827
Fragments arranged by size:
2827 736 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1500 1633 2092 2301 1500
Size: 133 459 209 3116
Fragments arranged by size:
3116 459 209 133
TatI w'GTAC_w
Cuts at: 3432 3432
Size: 3917
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 3885
Tth111I GACn'n_nGTC
Cuts at: 1714 1714
Size: 3917
XbaI T'CTAG_A
Cuts at: 948 948
Size: 3917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1412 1412
Size: 3917
XmaI C'CCGG_G
Cuts at: 719 937 719
Size: 218 3699
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI
BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI