Restriction map for
pF1KB6356
(Circular) (Six-base) MAPSORT of: pF1KB6356.seq Check: 7407 from: 1 to: 3998
pF1KB6356 3998 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 1036 1036
Size: 3998
Acc65I G'GTAC_C
Cuts at: 176 231 1014 176
Size: 55 783 3160
Fragments arranged by size:
3160 783 55
AclI AA'CG_TT
Cuts at: 394 3838 394
Size: 3444 554
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1823 2255 3002 3270 1823
Size: 432 747 268 2551
Fragments arranged by size:
2551 747 432 268
AflIII A'CryG_T
Cuts at: 944 1350 2454 3431 944
Size: 406 1104 977 1511
Fragments arranged by size:
1511 1104 977 406
AgeI A'CCGG_T
Cuts at: 2386 2386
Size: 3998
AlwNI CAG_nnn'CTG
Cuts at: 453 593 1303 2870 453
Size: 140 710 1567 1581
Fragments arranged by size:
1581 1567 710 140
ApaI G_GGCC'C
Cuts at: 206 737 977 206
Size: 531 240 3227
Fragments arranged by size:
3227 531 240
ApaLI G'TGCA_C
Cuts at: 2768 2768
Size: 3998
ApoI r'AATT_y
Cuts at: 337 1002 3351 3607 337
Size: 665 2349 256 728
Fragments arranged by size:
2349 728 665 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3998
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
AvaI C'yCGr_G
Cuts at: 205 538 785 1018 205
Size: 333 247 233 3185
Fragments arranged by size:
3185 333 247 233
AvrII C'CTAG_G
Cuts at: 399 399
Size: 3998
BamHI G'GATC_C
Cuts at: 1023 1023
Size: 3998
BanI G'GyrC_C
Cuts at: 176 213 231 321 502 637 1014 1428 1676
Size: 37 18 90 181 135 377 414 248
Cuts at: 1676 1711 176
Size: 35 2463
Fragments arranged by size:
2463 414 377 248 181 135 90 37
35 18
BanII G_rGCy'C
Cuts at: 206 737 977 1012 2042 206
Size: 531 240 35 1030 2162
Fragments arranged by size:
2162 1030 531 240 35
BbeI G_GCGC'C
Cuts at: 217 506 1432 1680 217
Size: 289 926 248 2535
Fragments arranged by size:
2535 926 289 248
BbsI GAAGACnn'nnnn_
Cuts at: 787 787
Size: 3998
BciVI GTATCCnnnnn_n'
Cuts at: 1896 2657 3998 1896
Size: 761 1341 1896
Fragments arranged by size:
1896 1341 761
BclI T'GATC_A
Cuts at: 354 1518 354
Size: 1164 2834
BglI GCCn_nnn'nGGC
Cuts at: 602 602
Size: 3998
BglII A'GATC_T
Cuts at: 1513 3550 1513
Size: 2037 1961
BlpI GC'TnA_GC
Cuts at: 380 1110 380
Size: 730 3268
Bme1580I G_kGCm'C
Cuts at: 206 224 737 977 1623 1716 2772 206
Size: 18 513 240 646 93 1056 1432
Fragments arranged by size:
1432 1056 646 513 240 93 18
BmrI ACTGGGnnnn_n'
Cuts at: 1210 1291 1626 3770 1210
Size: 81 335 2144 1438
Fragments arranged by size:
2144 1438 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 865 2365 865
Size: 1500 2498
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 592
Size: 3998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1174 2053 2545 2843 1174
Size: 879 492 298 2329
Fragments arranged by size:
2329 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 403 3702 403
Size: 3299 699
BsaAI yAC'GTr
Cuts at: 654 1351 654
Size: 697 3301
BsaBI GATnn'nnATC
Cuts at: 6 704 1512 3549 6
Size: 698 808 2037 455
Fragments arranged by size:
2037 808 698 455
BsaHI Gr'CG_yC
Cuts at: 214 503 1429 1677 2379 3875 214
Size: 289 926 248 702 1496 337
Fragments arranged by size:
1496 926 702 337 289 248
BsaWI w'CCGG_w
Cuts at: 1187 1708 2386 2660 2807 3218 1187
Size: 521 678 274 147 411 1967
Fragments arranged by size:
1967 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 484 977 484
Size: 493 3505
BseYI C'CCAG_C
Cuts at: 240 404 918 970 1423 2181 2758 3254 240
Size: 164 514 52 453 758 577 496 984
Fragments arranged by size:
984 758 577 514 496 453 164 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 559 367
Size: 192 3806
BsiEI CG_ry'CG
Cuts at: 104 467 514 708 1540 1586 2794 104
Size: 363 47 194 832 46 1208 1308
Fragments arranged by size:
1308 1208 832 363 194 47 46
BsiHKAI G_wGCw'C
Cuts at: 1012 1790 1980 2772 1012
Size: 778 190 792 2238
Fragments arranged by size:
2238 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 206 224 737 977 1012 1623 1716 1790 1980
Size: 18 513 240 35 611 93 74 190
Cuts at: 1980 2042 2772 206
Size: 62 730 1432
Fragments arranged by size:
1432 730 611 513 240 190 93 74
62 35 18
BspEI T'CCGG_A
Cuts at: 3218 3218
Size: 3998
BspHI T'CATG_A
Cuts at: 3174 3174
Size: 3998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 243 1048 1262 1564 1945 243
Size: 805 214 302 381 2296
Fragments arranged by size:
2296 805 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2290 2344 3828 3993 50
Size: 2240 54 1484 165 55
Fragments arranged by size:
2240 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1910 1910
Size: 3998
BsrFI r'CCGG_y
Cuts at: 186 509 1996 2177 2386 186
Size: 323 1487 181 209 1798
Fragments arranged by size:
1798 1487 323 209 181
BssSI C'ACGA_G
Cuts at: 2269 2627 2269
Size: 358 3640
BstAPI GCAn_nnn'nTGC
Cuts at: 482 593 1375 482
Size: 111 782 3105
Fragments arranged by size:
3105 782 111
BstBI TT'CG_AA
Cuts at: 2359 2359
Size: 3998
BstYI r'GATC_y
Cuts at: 1 1023 1490 1513 1848 2094 3106 3192 3204
Size: 1022 467 23 335 246 1012 86 12
Cuts at: 3204 3550 1
Size: 346 449
Fragments arranged by size:
1022 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 278 107
Size: 171 3827
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1948 2129 2292 1948
Size: 181 163 3654
Fragments arranged by size:
3654 181 163
BtsI GCAGTG_nn'
Cuts at: 310 807 829 1241 310
Size: 497 22 412 3067
Fragments arranged by size:
3067 497 412 22
DraI TTT'AAA
Cuts at: 997 997
Size: 3998
DrdI GACnn_nn'nnGTC
Cuts at: 1704 2562 1704
Size: 858 3140
EaeI y'GGCC_r
Cuts at: 464 511 530 648 1583 1757 2148 2175 3520
Size: 47 19 118 935 174 391 27 1345
Cuts at: 3520 464
Size: 942
Fragments arranged by size:
1345 942 935 391 174 118 47 27
19
EagI C'GGCC_G
Cuts at: 464 511 1583 464
Size: 47 1072 2879
Fragments arranged by size:
2879 1072 47
EarI CTCTTCn'nnn_
Cuts at: 265 2021 2231 265
Size: 1756 210 2032
Fragments arranged by size:
2032 1756 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 514 556 2206 2516 2662 3808 514
Size: 42 1650 310 146 1146 704
Fragments arranged by size:
1650 1146 704 310 146 42
EcoICRI GAG'CTC
Cuts at: 1010 1010
Size: 3998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 1823 2255 3002 3270 592
Size: 1231 432 747 268 1320
Fragments arranged by size:
1320 1231 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 457 457
Size: 3998
EcoO109I rG'GnC_Cy
Cuts at: 733 734 831 974 1137 733
Size: 1 97 143 163 3594
Fragments arranged by size:
3594 163 143 97 1
EcoRI G'AATT_C
Cuts at: 337 1002 337
Size: 665 3333
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2017 2017
Size: 3998
FspI TGC'GCA
Cuts at: 1779 1779
Size: 3998
HaeII r_GCGC'y
Cuts at: 193 217 506 971 1432 1680 2702 3681 193
Size: 24 289 465 461 248 1022 979 510
Fragments arranged by size:
1022 979 510 465 461 289 248 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 402 434 654 686 1840 1870 1872 1902 402
Size: 32 220 32 1154 30 2 30 2498
Fragments arranged by size:
2498 1154 220 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1037 1037
Size: 3998
KasI G'GCGC_C
Cuts at: 213 502 1428 1676 213
Size: 289 926 248 2535
Fragments arranged by size:
2535 926 289 248
KpnI G_GTAC'C
Cuts at: 180 235 1018 180
Size: 55 783 3160
Fragments arranged by size:
3160 783 55
MluI A'CGCG_T
Cuts at: 3431 3431
Size: 3998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2669 2853 2669
Size: 184 3814
MscI TGG'CCA
Cuts at: 650 1759 650
Size: 1109 2889
MslI CAynn'nnrTG
Cuts at: 1987 2114 1987
Size: 127 3871
MspA1I CmG'CkG
Cuts at: 516 573 590 885 1109 1423 1783 2796 3041
Size: 57 17 295 224 314 360 1013 245
Cuts at: 3041 516
Size: 1473
Fragments arranged by size:
1473 1013 360 314 295 245 224 57
17
NaeI GCC'GGC
Cuts at: 188 511 2179 188
Size: 323 1668 2007
Fragments arranged by size:
2007 1668 323
NarI GG'CG_CC
Cuts at: 214 503 1429 1677 214
Size: 289 926 248 2535
Fragments arranged by size:
2535 926 289 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3998
NgoMIV G'CCGG_C
Cuts at: 186 509 2177 186
Size: 323 1668 2007
Fragments arranged by size:
2007 1668 323
NspI r_CATG'y
Cuts at: 948 1051 1551 2458 948
Size: 103 500 907 2488
Fragments arranged by size:
2488 907 500 103
PasI CC'CwG_GG
Cuts at: 172 453 172
Size: 281 3717
PciI A'CATG_T
Cuts at: 944 2454 944
Size: 1510 2488
PflMI CCAn_nnn'nTGG
Cuts at: 615 615
Size: 3998
PfoI T'CCnGG_A
Cuts at: 608 695 608
Size: 87 3911
PmeI GTTT'AAAC
Cuts at: 997 997
Size: 3998
PmlI CAC'GTG
Cuts at: 654 654
Size: 3998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3137 3169 3137
Size: 32 3966
PspOMI G'GGCC_C
Cuts at: 202 733 973 202
Size: 531 240 3227
Fragments arranged by size:
3227 531 240
PstI C_TGCA'G
Cuts at: 589 1045 1730 589
Size: 456 685 2857
Fragments arranged by size:
2857 685 456
PvuI CG_AT'CG
Cuts at: 104 708 104
Size: 604 3394
PvuII CAG'CTG
Cuts at: 590 1423 1783 590
Size: 833 360 2805
Fragments arranged by size:
2805 833 360
RsrII CG'GwC_CG
Cuts at: 2193 2193
Size: 3998
SacI G_AGCT'C
Cuts at: 1012 1012
Size: 3998
SalI G'TCGA_C
Cuts at: 1035 1035
Size: 3998
SapI GCTCTTCn'nnn_
Cuts at: 2021 2231 2021
Size: 210 3788
SbfI CC_TGCA'GG
Cuts at: 1045 1045
Size: 3998
ScaI AGT'ACT
Cuts at: 3515 3515
Size: 3998
SfcI C'TryA_G
Cuts at: 33 585 658 874 1041 1726 2719 2910 33
Size: 552 73 216 167 685 993 191 1121
Fragments arranged by size:
1121 993 685 552 216 191 167 73
SfoI GGC'GCC
Cuts at: 215 504 1430 1678 215
Size: 289 926 248 2535
Fragments arranged by size:
2535 926 289 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
SmaI CCC'GGG
Cuts at: 207 540 1020 207
Size: 333 480 3185
Fragments arranged by size:
3185 480 333
SmlI C'TyrA_G
Cuts at: 1153 2068 2560 2822 1153
Size: 915 492 262 2329
Fragments arranged by size:
2329 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3998
SphI G_CATG'C
Cuts at: 1051 1551 1051
Size: 500 3498
SrfI GCCC'GGGC
Cuts at: 207 207
Size: 3998
StyI C'CwwG_G
Cuts at: 107 284 399 1132 1486 107
Size: 177 115 733 354 2619
Fragments arranged by size:
2619 733 354 177 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1581 1714 2173 2382 1581
Size: 133 459 209 3197
Fragments arranged by size:
3197 459 209 133
TatI w'GTAC_w
Cuts at: 621 3513 621
Size: 2892 1106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3515 3547 3515
Size: 32 3966
Tth111I GACn'n_nGTC
Cuts at: 1795 1795
Size: 3998
XbaI T'CTAG_A
Cuts at: 1029 1029
Size: 3998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1493 1493
Size: 3998
XmaI C'CCGG_G
Cuts at: 205 538 1018 205
Size: 333 480 3185
Fragments arranged by size:
3185 480 333
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SspI StuI SwaI XhoI
XmnI ZraI