Restriction map for
pF1KB6352
(Circular) (Six-base) MAPSORT of: pF1KB6352.seq Check: 6019 from: 1 to: 3992
pF1KB6352 3992 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 1030 1030
Size: 3992
Acc65I G'GTAC_C
Cuts at: 1008 1008
Size: 3992
AclI AA'CG_TT
Cuts at: 3832 3832
Size: 3992
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1817 2249 2996 3264 1817
Size: 432 747 268 2545
Fragments arranged by size:
2545 747 432 268
AflIII A'CryG_T
Cuts at: 1344 2448 3425 1344
Size: 1104 977 1911
Fragments arranged by size:
1911 1104 977
AgeI A'CCGG_T
Cuts at: 2380 2380
Size: 3992
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 475 475
Size: 3992
AlwNI CAG_nnn'CTG
Cuts at: 322 1297 2864 322
Size: 975 1567 1450
Fragments arranged by size:
1567 1450 975
ApaLI G'TGCA_C
Cuts at: 2762 2762
Size: 3992
ApoI r'AATT_y
Cuts at: 996 3345 3601 996
Size: 2349 256 1387
Fragments arranged by size:
2349 1387 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3992
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3992
AvaI C'yCGr_G
Cuts at: 599 1012 599
Size: 413 3579
BamHI G'GATC_C
Cuts at: 632 1017 632
Size: 385 3607
BanI G'GyrC_C
Cuts at: 728 1008 1422 1670 1705 728
Size: 280 414 248 35 3015
Fragments arranged by size:
3015 414 280 248 35
BanII G_rGCy'C
Cuts at: 158 1006 2036 158
Size: 848 1030 2114
Fragments arranged by size:
2114 1030 848
BbeI G_GCGC'C
Cuts at: 732 1426 1674 732
Size: 694 248 3050
Fragments arranged by size:
3050 694 248
BbsI GAAGACnn'nnnn_
Cuts at: 232 448 908 232
Size: 216 460 3316
Fragments arranged by size:
3316 460 216
BciVI GTATCCnnnnn_n'
Cuts at: 830 1890 2651 3992 830
Size: 1060 761 1341 830
Fragments arranged by size:
1341 1060 830 761
BclI T'GATC_A
Cuts at: 167 1512 167
Size: 1345 2647
BglII A'GATC_T
Cuts at: 1507 3544 1507
Size: 2037 1955
BlpI GC'TnA_GC
Cuts at: 1104 1104
Size: 3992
Bme1580I G_kGCm'C
Cuts at: 1617 1710 2766 1617
Size: 93 1056 2843
Fragments arranged by size:
2843 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 744 1204 1285 1620 3764 744
Size: 460 81 335 2144 972
Fragments arranged by size:
2144 972 460 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 3992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 602 602
Size: 3992
Bpu10I CC'TnA_GC
Cuts at: 721 721
Size: 3992
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1168 2047 2539 2837 1168
Size: 879 492 298 2323
Fragments arranged by size:
2323 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 418 3696 418
Size: 3278 714
BsaAI yAC'GTr
Cuts at: 570 957 1345 570
Size: 387 388 3217
Fragments arranged by size:
3217 388 387
BsaBI GATnn'nnATC
Cuts at: 6 1506 3543 6
Size: 1500 2037 455
Fragments arranged by size:
2037 1500 455
BsaHI Gr'CG_yC
Cuts at: 729 1423 1671 2373 3869 729
Size: 694 248 702 1496 852
Fragments arranged by size:
1496 852 702 694 248
BsaWI w'CCGG_w
Cuts at: 272 1181 1702 2380 2654 2801 3212 272
Size: 909 521 678 274 147 411 1052
Fragments arranged by size:
1052 909 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 554 575 584 605 554
Size: 21 9 21 3941
Fragments arranged by size:
3941 21 21 9
BseYI C'CCAG_C
Cuts at: 1417 2175 2752 3248 1417
Size: 758 577 496 2161
Fragments arranged by size:
2161 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1534 1580 2788 104
Size: 1430 46 1208 1308
Fragments arranged by size:
1430 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 158 1006 1784 1974 2766 158
Size: 848 778 190 792 1384
Fragments arranged by size:
1384 848 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 763 763
Size: 3992
Bsp1286I G_dGCh'C
Cuts at: 158 1006 1617 1710 1784 1974 2036 2766 158
Size: 848 611 93 74 190 62 730 1384
Fragments arranged by size:
1384 848 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3212 3212
Size: 3992
BspHI T'CATG_A
Cuts at: 3168 3168
Size: 3992
BspMI ACCTGCnnnn'nnnn_
Cuts at: 841 1042 1256 1558 1939 841
Size: 201 214 302 381 2894
Fragments arranged by size:
2894 381 302 214 201
BsrBI CCG'CTC
Cuts at: 50 237 2284 2338 3822 3987 50
Size: 187 2047 54 1484 165 55
Fragments arranged by size:
2047 1484 187 165 55 54
BsrDI GCAATG_nn'
Cuts at: 134 1904 134
Size: 1770 2222
BsrFI r'CCGG_y
Cuts at: 409 725 1990 2171 2380 409
Size: 316 1265 181 209 2021
Fragments arranged by size:
2021 1265 316 209 181
BssSI C'ACGA_G
Cuts at: 115 805 2263 2621 115
Size: 690 1458 358 1486
Fragments arranged by size:
1486 1458 690 358
BstAPI GCAn_nnn'nTGC
Cuts at: 807 1369 807
Size: 562 3430
BstBI TT'CG_AA
Cuts at: 2353 2353
Size: 3992
BstYI r'GATC_y
Cuts at: 1 632 1017 1484 1507 1842 2088 3100 3186
Size: 631 385 467 23 335 246 1012 86
Cuts at: 3186 3198 3544 1
Size: 12 346 449
Fragments arranged by size:
1012 631 467 449 385 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 558 779 558
Size: 221 3771
BtgI C'CryG_G
Cuts at: 107 611 107
Size: 504 3488
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1942 2123 2286 1942
Size: 181 163 3648
Fragments arranged by size:
3648 181 163
BtsI GCAGTG_nn'
Cuts at: 634 1235 634
Size: 601 3391
ClaI AT'CG_AT
Cuts at: 692 692
Size: 3992
DraI TTT'AAA
Cuts at: 991 991
Size: 3992
DrdI GACnn_nn'nnGTC
Cuts at: 889 1698 2556 889
Size: 809 858 2325
Fragments arranged by size:
2325 858 809
EaeI y'GGCC_r
Cuts at: 398 485 572 638 1577 1751 2142 2169 3514
Size: 87 87 66 939 174 391 27 1345
Cuts at: 3514 398
Size: 876
Fragments arranged by size:
1345 939 876 391 174 87 87 66
27
EagI C'GGCC_G
Cuts at: 1577 1577
Size: 3992
EarI CTCTTCn'nnn_
Cuts at: 179 377 2015 2225 179
Size: 198 1638 210 1946
Fragments arranged by size:
1946 1638 210 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 405 2200 2510 2656 3802 405
Size: 1795 310 146 1146 595
Fragments arranged by size:
1795 1146 595 310 146
EcoICRI GAG'CTC
Cuts at: 156 1004 156
Size: 848 3144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 602 1817 2249 2996 3264 602
Size: 1215 432 747 268 1330
Fragments arranged by size:
1330 1215 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 557 640 557
Size: 83 3909
EcoO109I rG'GnC_Cy
Cuts at: 1131 1131
Size: 3992
EcoRI G'AATT_C
Cuts at: 996 996
Size: 3992
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2011 2011
Size: 3992
FspI TGC'GCA
Cuts at: 451 1773 451
Size: 1322 2670
HaeII r_GCGC'y
Cuts at: 732 1426 1674 2696 3675 732
Size: 694 248 1022 979 1049
Fragments arranged by size:
1049 1022 979 694 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 396 428 871 903 1834 1864 1866 1896 396
Size: 32 443 32 931 30 2 30 2492
Fragments arranged by size:
2492 931 443 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1031 1031
Size: 3992
KasI G'GCGC_C
Cuts at: 728 1422 1670 728
Size: 694 248 3050
Fragments arranged by size:
3050 694 248
KpnI G_GTAC'C
Cuts at: 1012 1012
Size: 3992
MluI A'CGCG_T
Cuts at: 3425 3425
Size: 3992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 359 2663 2847 359
Size: 2304 184 1504
Fragments arranged by size:
2304 1504 184
MscI TGG'CCA
Cuts at: 574 1753 574
Size: 1179 2813
MslI CAynn'nnrTG
Cuts at: 1981 2108 1981
Size: 127 3865
MspA1I CmG'CkG
Cuts at: 165 744 870 1103 1417 1777 2790 3035 165
Size: 579 126 233 314 360 1013 245 1122
Fragments arranged by size:
1122 1013 579 360 314 245 233 126
NaeI GCC'GGC
Cuts at: 727 2173 727
Size: 1446 2546
NarI GG'CG_CC
Cuts at: 729 1423 1671 729
Size: 694 248 3050
Fragments arranged by size:
3050 694 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3992
NgoMIV G'CCGG_C
Cuts at: 725 2171 725
Size: 1446 2546
NspI r_CATG'y
Cuts at: 1045 1545 2452 1045
Size: 500 907 2585
Fragments arranged by size:
2585 907 500
PciI A'CATG_T
Cuts at: 2448 2448
Size: 3992
PmeI GTTT'AAAC
Cuts at: 991 991
Size: 3992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 772 804 3131 3163 772
Size: 32 2327 32 1601
Fragments arranged by size:
2327 1601 32 32
PstI C_TGCA'G
Cuts at: 479 1039 1724 479
Size: 560 685 2747
Fragments arranged by size:
2747 685 560
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3992
PvuII CAG'CTG
Cuts at: 165 1417 1777 165
Size: 1252 360 2380
Fragments arranged by size:
2380 1252 360
RsrII CG'GwC_CG
Cuts at: 2187 2187
Size: 3992
SacI G_AGCT'C
Cuts at: 158 1006 158
Size: 848 3144
SalI G'TCGA_C
Cuts at: 1029 1029
Size: 3992
SapI GCTCTTCn'nnn_
Cuts at: 179 2015 2225 179
Size: 1836 210 1946
Fragments arranged by size:
1946 1836 210
SbfI CC_TGCA'GG
Cuts at: 1039 1039
Size: 3992
ScaI AGT'ACT
Cuts at: 3509 3509
Size: 3992
SfcI C'TryA_G
Cuts at: 33 475 1035 1720 2713 2904 33
Size: 442 560 685 993 191 1121
Fragments arranged by size:
1121 993 685 560 442 191
SfoI GGC'GCC
Cuts at: 730 1424 1672 730
Size: 694 248 3050
Fragments arranged by size:
3050 694 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3992
SmaI CCC'GGG
Cuts at: 601 1014 601
Size: 413 3579
SmlI C'TyrA_G
Cuts at: 1147 2062 2554 2816 1147
Size: 915 492 262 2323
Fragments arranged by size:
2323 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3992
SphI G_CATG'C
Cuts at: 1045 1545 1045
Size: 500 3492
StuI AGG'CCT
Cuts at: 784 784
Size: 3992
StyI C'CwwG_G
Cuts at: 107 305 1126 1480 107
Size: 198 821 354 2619
Fragments arranged by size:
2619 821 354 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 879 1575 1708 2167 2376 879
Size: 696 133 459 209 2495
Fragments arranged by size:
2495 696 459 209 133
TatI w'GTAC_w
Cuts at: 3507 3507
Size: 3992
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 504 536 554 575 586 607 613 645 3509
Size: 32 18 21 11 21 6 32 2864
Cuts at: 3509 3541 504
Size: 32 955
Fragments arranged by size:
2864 955 32 32 32 21 21 18
11 6
Tth111I GACn'n_nGTC
Cuts at: 1789 1789
Size: 3992
XbaI T'CTAG_A
Cuts at: 1023 1023
Size: 3992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1487 1487
Size: 3992
XmaI C'CCGG_G
Cuts at: 599 1012 599
Size: 413 3579
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BseRI BsgI BsiWI BsmI BsrGI BssHII BstEII
BstXI BstZ17I CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI