Restriction map for
pF1KB6350
(Circular) (Six-base) MAPSORT of: pF1KB6350.seq Check: 341 from: 1 to: 3992
pF1KB6350 3992 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 1030 1030
Size: 3992
Acc65I G'GTAC_C
Cuts at: 1008 1008
Size: 3992
AclI AA'CG_TT
Cuts at: 3832 3832
Size: 3992
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1817 2249 2996 3264 1817
Size: 432 747 268 2545
Fragments arranged by size:
2545 747 432 268
AflIII A'CryG_T
Cuts at: 1344 2448 3425 1344
Size: 1104 977 1911
Fragments arranged by size:
1911 1104 977
AgeI A'CCGG_T
Cuts at: 2380 2380
Size: 3992
AlwNI CAG_nnn'CTG
Cuts at: 1297 2864 1297
Size: 1567 2425
ApaLI G'TGCA_C
Cuts at: 2762 2762
Size: 3992
ApoI r'AATT_y
Cuts at: 996 3345 3601 996
Size: 2349 256 1387
Fragments arranged by size:
2349 1387 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3992
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3992
AvaI C'yCGr_G
Cuts at: 1012 1012
Size: 3992
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 324 357 324
Size: 33 3959
BamHI G'GATC_C
Cuts at: 1017 1017
Size: 3992
BanI G'GyrC_C
Cuts at: 189 682 1008 1422 1670 1705 189
Size: 493 326 414 248 35 2476
Fragments arranged by size:
2476 493 414 326 248 35
BanII G_rGCy'C
Cuts at: 1006 2036 1006
Size: 1030 2962
BbeI G_GCGC'C
Cuts at: 1426 1674 1426
Size: 248 3744
BbsI GAAGACnn'nnnn_
Cuts at: 661 661
Size: 3992
BbvCI CC'TCA_GC
Cuts at: 466 536 466
Size: 70 3922
BciVI GTATCCnnnnn_n'
Cuts at: 192 1890 2651 3992 192
Size: 1698 761 1341 192
Fragments arranged by size:
1698 1341 761 192
BclI T'GATC_A
Cuts at: 1512 1512
Size: 3992
BglII A'GATC_T
Cuts at: 475 1507 3544 475
Size: 1032 2037 923
Fragments arranged by size:
2037 1032 923
BlpI GC'TnA_GC
Cuts at: 1104 1104
Size: 3992
Bme1580I G_kGCm'C
Cuts at: 1617 1710 2766 1617
Size: 93 1056 2843
Fragments arranged by size:
2843 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 169 1204 1285 1620 3764 169
Size: 1035 81 335 2144 397
Fragments arranged by size:
2144 1035 397 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 426 801 2359 426
Size: 375 1558 2059
Fragments arranged by size:
2059 1558 375
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 476 796 396
Size: 80 320 3592
Fragments arranged by size:
3592 320 80
Bpu10I CC'TnA_GC
Cuts at: 466 536 734 466
Size: 70 198 3724
Fragments arranged by size:
3724 198 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1168 2047 2539 2837 1168
Size: 879 492 298 2323
Fragments arranged by size:
2323 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 397 3696 397
Size: 3299 693
BsaAI yAC'GTr
Cuts at: 1345 1345
Size: 3992
BsaBI GATnn'nnATC
Cuts at: 6 1506 3543 6
Size: 1500 2037 455
Fragments arranged by size:
2037 1500 455
BsaHI Gr'CG_yC
Cuts at: 1423 1671 2373 3869 1423
Size: 248 702 1496 1546
Fragments arranged by size:
1546 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1181 1702 2380 2654 2801 3212 1181
Size: 521 678 274 147 411 1961
Fragments arranged by size:
1961 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 341 371 341
Size: 30 3962
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 473 483 597 955 473
Size: 10 114 358 3510
Fragments arranged by size:
3510 358 114 10
BseYI C'CCAG_C
Cuts at: 1417 2175 2752 3248 1417
Size: 758 577 496 2161
Fragments arranged by size:
2161 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1534 1580 2788 104
Size: 1430 46 1208 1308
Fragments arranged by size:
1430 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 740 1006 1784 1974 2766 740
Size: 266 778 190 792 1966
Fragments arranged by size:
1966 792 778 266 190
Bsp1286I G_dGCh'C
Cuts at: 740 1006 1617 1710 1784 1974 2036 2766 740
Size: 266 611 93 74 190 62 730 1966
Fragments arranged by size:
1966 730 611 266 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3212 3212
Size: 3992
BspHI T'CATG_A
Cuts at: 666 3168 666
Size: 2502 1490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1042 1256 1558 1939 1042
Size: 214 302 381 3095
Fragments arranged by size:
3095 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2284 2338 3822 3987 50
Size: 2234 54 1484 165 55
Fragments arranged by size:
2234 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 263 503 764 1904 263
Size: 240 261 1140 2351
Fragments arranged by size:
2351 1140 261 240
BsrFI r'CCGG_y
Cuts at: 1990 2171 2380 1990
Size: 181 209 3602
Fragments arranged by size:
3602 209 181
BssSI C'ACGA_G
Cuts at: 579 2263 2621 579
Size: 1684 358 1950
Fragments arranged by size:
1950 1684 358
BstAPI GCAn_nnn'nTGC
Cuts at: 274 1369 274
Size: 1095 2897
BstBI TT'CG_AA
Cuts at: 2353 2353
Size: 3992
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3992
BstYI r'GATC_y
Cuts at: 1 475 1017 1484 1507 1842 2088 3100 3186
Size: 474 542 467 23 335 246 1012 86
Cuts at: 3186 3198 3544 1
Size: 12 346 449
Fragments arranged by size:
1012 542 474 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 234 107
Size: 127 3865
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 127 1942 2123 2286 127
Size: 1815 181 163 1833
Fragments arranged by size:
1833 1815 181 163
BtsI GCAGTG_nn'
Cuts at: 554 1235 554
Size: 681 3311
DraI TTT'AAA
Cuts at: 991 991
Size: 3992
DrdI GACnn_nn'nnGTC
Cuts at: 565 1698 2556 565
Size: 1133 858 2001
Fragments arranged by size:
2001 1133 858
EaeI y'GGCC_r
Cuts at: 1577 1751 2142 2169 3514 1577
Size: 174 391 27 1345 2055
Fragments arranged by size:
2055 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1577 1577
Size: 3992
EarI CTCTTCn'nnn_
Cuts at: 248 382 740 2015 2225 248
Size: 134 358 1275 210 2015
Fragments arranged by size:
2015 1275 358 210 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2200 2510 2656 3802 2200
Size: 310 146 1146 2390
Fragments arranged by size:
2390 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1004 1004
Size: 3992
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 476 796 1817 2249 2996 3264 396
Size: 80 320 1021 432 747 268 1124
Fragments arranged by size:
1124 1021 747 432 320 268 80
EcoO109I rG'GnC_Cy
Cuts at: 522 1131 522
Size: 609 3383
EcoRI G'AATT_C
Cuts at: 996 996
Size: 3992
EcoRV GAT'ATC
Cuts at: 809 809
Size: 3992
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 508 541 678 913 2011 508
Size: 33 137 235 1098 2489
Fragments arranged by size:
2489 1098 235 137 33
FspI TGC'GCA
Cuts at: 1773 1773
Size: 3992
HaeII r_GCGC'y
Cuts at: 1426 1674 2696 3675 1426
Size: 248 1022 979 1743
Fragments arranged by size:
1743 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 793 825 1834 1864 1866 1896 793
Size: 32 1009 30 2 30 2889
Fragments arranged by size:
2889 1009 32 30 30 2
HincII GTy'rAC
Cuts at: 1031 1031
Size: 3992
HindIII A'AGCT_T
Cuts at: 317 317
Size: 3992
KasI G'GCGC_C
Cuts at: 1422 1670 1422
Size: 248 3744
KpnI G_GTAC'C
Cuts at: 1012 1012
Size: 3992
MluI A'CGCG_T
Cuts at: 3425 3425
Size: 3992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2663 2847 2663
Size: 184 3808
MscI TGG'CCA
Cuts at: 1753 1753
Size: 3992
MslI CAynn'nnrTG
Cuts at: 112 358 665 1981 2108 112
Size: 246 307 1316 127 1996
Fragments arranged by size:
1996 1316 307 246 127
MspA1I CmG'CkG
Cuts at: 1103 1417 1777 2790 3035 1103
Size: 314 360 1013 245 2060
Fragments arranged by size:
2060 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2173 2173
Size: 3992
NarI GG'CG_CC
Cuts at: 1423 1671 1423
Size: 248 3744
NcoI C'CATG_G
Cuts at: 107 234 107
Size: 127 3865
NgoMIV G'CCGG_C
Cuts at: 2171 2171
Size: 3992
NspI r_CATG'y
Cuts at: 1045 1545 2452 1045
Size: 500 907 2585
Fragments arranged by size:
2585 907 500
PciI A'CATG_T
Cuts at: 2448 2448
Size: 3992
PfoI T'CCnGG_A
Cuts at: 453 453
Size: 3992
PmeI GTTT'AAAC
Cuts at: 991 991
Size: 3992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3131 3163 3131
Size: 32 3960
PpuMI rG'GwC_Cy
Cuts at: 522 522
Size: 3992
PstI C_TGCA'G
Cuts at: 483 1039 1724 483
Size: 556 685 2751
Fragments arranged by size:
2751 685 556
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3992
PvuII CAG'CTG
Cuts at: 1417 1777 1417
Size: 360 3632
RsrII CG'GwC_CG
Cuts at: 2187 2187
Size: 3992
SacI G_AGCT'C
Cuts at: 1006 1006
Size: 3992
SalI G'TCGA_C
Cuts at: 1029 1029
Size: 3992
SapI GCTCTTCn'nnn_
Cuts at: 2015 2225 2015
Size: 210 3782
SbfI CC_TGCA'GG
Cuts at: 1039 1039
Size: 3992
ScaI AGT'ACT
Cuts at: 3509 3509
Size: 3992
SfcI C'TryA_G
Cuts at: 33 479 1035 1720 2713 2904 33
Size: 446 556 685 993 191 1121
Fragments arranged by size:
1121 993 685 556 446 191
SfoI GGC'GCC
Cuts at: 1424 1672 1424
Size: 248 3744
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3992
SmaI CCC'GGG
Cuts at: 1014 1014
Size: 3992
SmlI C'TyrA_G
Cuts at: 1147 2062 2554 2816 1147
Size: 915 492 262 2323
Fragments arranged by size:
2323 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3992
SphI G_CATG'C
Cuts at: 1045 1545 1045
Size: 500 3492
StyI C'CwwG_G
Cuts at: 107 234 1126 1480 107
Size: 127 892 354 2619
Fragments arranged by size:
2619 892 354 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 414 1575 1708 2167 2376 414
Size: 1161 133 459 209 2030
Fragments arranged by size:
2030 1161 459 209 133
TatI w'GTAC_w
Cuts at: 878 3507 878
Size: 2629 1363
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3509 3541 3509
Size: 32 3960
Tth111I GACn'n_nGTC
Cuts at: 1789 1789
Size: 3992
XbaI T'CTAG_A
Cuts at: 1023 1023
Size: 3992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 414 1487 414
Size: 1073 2919
XmaI C'CCGG_G
Cuts at: 1012 1012
Size: 3992
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BcgI BfrBI BglI BmgBI BmtI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI