Restriction map for
pF1KB6349
(Circular) (Six-base) MAPSORT of: pF1KB6349.seq Check: 3709 from: 1 to: 3911
pF1KB6349 3911 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 718 718
Size: 3911
AccI GT'mk_AC
Cuts at: 949 949
Size: 3911
Acc65I G'GTAC_C
Cuts at: 927 927
Size: 3911
AclI AA'CG_TT
Cuts at: 3751 3751
Size: 3911
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 840 868 1736 2168 2915 3183 139
Size: 701 28 868 432 747 268 867
Fragments arranged by size:
868 867 747 701 432 268 28
AfeI AGC'GCT
Cuts at: 149 149
Size: 3911
AflIII A'CryG_T
Cuts at: 1263 2367 3344 1263
Size: 1104 977 1830
Fragments arranged by size:
1830 1104 977
AgeI A'CCGG_T
Cuts at: 2299 2299
Size: 3911
AlwNI CAG_nnn'CTG
Cuts at: 519 666 1216 2783 519
Size: 147 550 1567 1647
Fragments arranged by size:
1647 1567 550 147
ApaLI G'TGCA_C
Cuts at: 2681 2681
Size: 3911
ApoI r'AATT_y
Cuts at: 915 3264 3520 915
Size: 2349 256 1306
Fragments arranged by size:
2349 1306 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3911
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3911
AvaI C'yCGr_G
Cuts at: 931 931
Size: 3911
BamHI G'GATC_C
Cuts at: 936 936
Size: 3911
BanI G'GyrC_C
Cuts at: 927 1341 1589 1624 927
Size: 414 248 35 3214
Fragments arranged by size:
3214 414 248 35
BanII G_rGCy'C
Cuts at: 178 925 1955 178
Size: 747 1030 2134
Fragments arranged by size:
2134 1030 747
BbeI G_GCGC'C
Cuts at: 1345 1593 1345
Size: 248 3663
BciVI GTATCCnnnnn_n'
Cuts at: 1809 2570 3911 1809
Size: 761 1341 1809
Fragments arranged by size:
1809 1341 761
BclI T'GATC_A
Cuts at: 1431 1431
Size: 3911
BglII A'GATC_T
Cuts at: 1426 3463 1426
Size: 2037 1874
BlpI GC'TnA_GC
Cuts at: 172 1023 172
Size: 851 3060
Bme1580I G_kGCm'C
Cuts at: 1536 1629 2685 1536
Size: 93 1056 2762
Fragments arranged by size:
2762 1056 93
BmgBI CAC'GTC
Cuts at: 324 324
Size: 3911
BmrI ACTGGGnnnn_n'
Cuts at: 1123 1204 1539 3683 1123
Size: 81 335 2144 1351
Fragments arranged by size:
2144 1351 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2278 2278
Size: 3911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 418 149
Size: 269 3642
Bpu10I CC'TnA_GC
Cuts at: 901 901
Size: 3911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1087 1966 2458 2756 1087
Size: 879 492 298 2242
Fragments arranged by size:
2242 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3615 3615
Size: 3911
BsaAI yAC'GTr
Cuts at: 1264 1264
Size: 3911
BsaBI GATnn'nnATC
Cuts at: 6 1425 3462 6
Size: 1419 2037 455
Fragments arranged by size:
2037 1419 455
BsaHI Gr'CG_yC
Cuts at: 1342 1590 2292 3788 1342
Size: 248 702 1496 1465
Fragments arranged by size:
1496 1465 702 248
BsaWI w'CCGG_w
Cuts at: 1100 1621 2299 2573 2720 3131 1100
Size: 521 678 274 147 411 1880
Fragments arranged by size:
1880 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 313 313
Size: 3911
BseYI C'CCAG_C
Cuts at: 351 512 613 816 1336 2094 2671 3167 351
Size: 161 101 203 520 758 577 496 1095
Fragments arranged by size:
1095 758 577 520 496 203 161 101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 761 487
Size: 274 3637
BsiEI CG_ry'CG
Cuts at: 104 1453 1499 2707 104
Size: 1349 46 1208 1308
Fragments arranged by size:
1349 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 178 725 765 925 1703 1893 2685 178
Size: 547 40 160 778 190 792 1404
Fragments arranged by size:
1404 792 778 547 190 160 40
Bsp1286I G_dGCh'C
Cuts at: 178 725 765 925 1536 1629 1703 1893 1955
Size: 547 40 160 611 93 74 190 62
Cuts at: 1955 2685 178
Size: 730 1404
Fragments arranged by size:
1404 730 611 547 190 160 93 74
62 40
BspEI T'CCGG_A
Cuts at: 3131 3131
Size: 3911
BspHI T'CATG_A
Cuts at: 3087 3087
Size: 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 718 961 1175 1477 1858 718
Size: 243 214 302 381 2771
Fragments arranged by size:
2771 381 302 243 214
BsrBI CCG'CTC
Cuts at: 50 261 2203 2257 3741 3906 50
Size: 211 1942 54 1484 165 55
Fragments arranged by size:
1942 1484 211 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1823 1823
Size: 3911
BsrFI r'CCGG_y
Cuts at: 1909 2090 2299 1909
Size: 181 209 3521
Fragments arranged by size:
3521 209 181
BssSI C'ACGA_G
Cuts at: 2182 2540 2182
Size: 358 3553
BstAPI GCAn_nnn'nTGC
Cuts at: 1288 1288
Size: 3911
BstBI TT'CG_AA
Cuts at: 2272 2272
Size: 3911
BstYI r'GATC_y
Cuts at: 1 936 1403 1426 1761 2007 3019 3105 3117
Size: 935 467 23 335 246 1012 86 12
Cuts at: 3117 3463 1
Size: 346 449
Fragments arranged by size:
1012 935 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 485 485
Size: 3911
BtgI C'CryG_G
Cuts at: 107 413 107
Size: 306 3605
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1861 2042 2205 1861
Size: 181 163 3567
Fragments arranged by size:
3567 181 163
BtsI GCAGTG_nn'
Cuts at: 1154 1154
Size: 3911
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 170 205 170
Size: 35 3876
DraI TTT'AAA
Cuts at: 910 910
Size: 3911
DrdI GACnn_nn'nnGTC
Cuts at: 1617 2475 1617
Size: 858 3053
EaeI y'GGCC_r
Cuts at: 597 1496 1670 2061 2088 3433 597
Size: 899 174 391 27 1345 1075
Fragments arranged by size:
1345 1075 899 391 174 27
EagI C'GGCC_G
Cuts at: 1496 1496
Size: 3911
EarI CTCTTCn'nnn_
Cuts at: 599 832 1934 2144 599
Size: 233 1102 210 2366
Fragments arranged by size:
2366 1102 233 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2119 2429 2575 3721 2119
Size: 310 146 1146 2309
Fragments arranged by size:
2309 1146 310 146
EcoICRI GAG'CTC
Cuts at: 176 923 176
Size: 747 3164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 149 418 840 868 1736 2168 2915 3183
Size: 10 269 422 28 868 432 747 268
Cuts at: 3183 139
Size: 867
Fragments arranged by size:
868 867 747 432 422 269 268 28
10
EcoNI CCTnn'n_nnAGG
Cuts at: 400 400
Size: 3911
EcoO109I rG'GnC_Cy
Cuts at: 1050 1050
Size: 3911
EcoRI G'AATT_C
Cuts at: 915 915
Size: 3911
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1930 1930
Size: 3911
FspI TGC'GCA
Cuts at: 1692 1692
Size: 3911
HaeII r_GCGC'y
Cuts at: 151 1345 1593 2615 3594 151
Size: 1194 248 1022 979 468
Fragments arranged by size:
1194 1022 979 468 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 222 254 483 515 726 758 1753 1783 1785
Size: 32 229 32 211 32 995 30 2
Cuts at: 1785 1815 222
Size: 30 2318
Fragments arranged by size:
2318 995 229 211 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 950 950
Size: 3911
KasI G'GCGC_C
Cuts at: 1341 1589 1341
Size: 248 3663
KpnI G_GTAC'C
Cuts at: 931 931
Size: 3911
MluI A'CGCG_T
Cuts at: 3344 3344
Size: 3911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 504 2582 2766 504
Size: 2078 184 1649
Fragments arranged by size:
2078 1649 184
MscI TGG'CCA
Cuts at: 1672 1672
Size: 3911
MslI CAynn'nnrTG
Cuts at: 1900 2027 1900
Size: 127 3784
MspA1I CmG'CkG
Cuts at: 415 471 1022 1336 1696 2709 2954 415
Size: 56 551 314 360 1013 245 1372
Fragments arranged by size:
1372 1013 551 360 314 245 56
NaeI GCC'GGC
Cuts at: 2092 2092
Size: 3911
NarI GG'CG_CC
Cuts at: 1342 1590 1342
Size: 248 3663
NcoI C'CATG_G
Cuts at: 107 107
Size: 3911
NgoMIV G'CCGG_C
Cuts at: 2090 2090
Size: 3911
NspI r_CATG'y
Cuts at: 964 1464 2371 964
Size: 500 907 2504
Fragments arranged by size:
2504 907 500
PciI A'CATG_T
Cuts at: 2367 2367
Size: 3911
PflMI CCAn_nnn'nTGG
Cuts at: 666 666
Size: 3911
PfoI T'CCnGG_A
Cuts at: 272 395 272
Size: 123 3788
PmeI GTTT'AAAC
Cuts at: 910 910
Size: 3911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3050 3082 3050
Size: 32 3879
PstI C_TGCA'G
Cuts at: 433 524 958 1643 433
Size: 91 434 685 2701
Fragments arranged by size:
2701 685 434 91
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3911
PvuII CAG'CTG
Cuts at: 1336 1696 1336
Size: 360 3551
RsrII CG'GwC_CG
Cuts at: 2106 2106
Size: 3911
SacI G_AGCT'C
Cuts at: 178 925 178
Size: 747 3164
SacII CC_GC'GG
Cuts at: 416 416
Size: 3911
SalI G'TCGA_C
Cuts at: 948 948
Size: 3911
SapI GCTCTTCn'nnn_
Cuts at: 832 1934 2144 832
Size: 1102 210 2599
Fragments arranged by size:
2599 1102 210
SbfI CC_TGCA'GG
Cuts at: 958 958
Size: 3911
ScaI AGT'ACT
Cuts at: 373 3428 373
Size: 3055 856
SfcI C'TryA_G
Cuts at: 33 429 520 954 1639 2632 2823 33
Size: 396 91 434 685 993 191 1121
Fragments arranged by size:
1121 993 685 434 396 191 91
SfoI GGC'GCC
Cuts at: 1343 1591 1343
Size: 248 3663
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3911
SmaI CCC'GGG
Cuts at: 933 933
Size: 3911
SmlI C'TyrA_G
Cuts at: 1066 1981 2473 2735 1066
Size: 915 492 262 2242
Fragments arranged by size:
2242 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3911
SphI G_CATG'C
Cuts at: 964 1464 964
Size: 500 3411
StuI AGG'CCT
Cuts at: 632 632
Size: 3911
StyI C'CwwG_G
Cuts at: 107 831 1045 1399 107
Size: 724 214 354 2619
Fragments arranged by size:
2619 724 354 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 740 1494 1627 2086 2295 740
Size: 754 133 459 209 2356
Fragments arranged by size:
2356 754 459 209 133
TatI w'GTAC_w
Cuts at: 371 3426 371
Size: 3055 856
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3428 3460 3428
Size: 32 3879
Tth111I GACn'n_nGTC
Cuts at: 1708 1708
Size: 3911
XbaI T'CTAG_A
Cuts at: 942 942
Size: 3911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 215 1406 215
Size: 1191 2720
XmaI C'CCGG_G
Cuts at: 931 931
Size: 3911
XmnI GAAnn'nnTTC
Cuts at: 683 683
Size: 3911
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI