Restriction map for
pF1KB6342
(Circular) (Six-base) MAPSORT of: pF1KB6342.seq Check: 5115 from: 1 to: 3986
pF1KB6342 3986 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 798 798
Size: 3986
AccI GT'mk_AC
Cuts at: 1024 1024
Size: 3986
Acc65I G'GTAC_C
Cuts at: 1002 1002
Size: 3986
AclI AA'CG_TT
Cuts at: 3826 3826
Size: 3986
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1811 2243 2990 3258 1811
Size: 432 747 268 2539
Fragments arranged by size:
2539 747 432 268
AflIII A'CryG_T
Cuts at: 1338 2442 3419 1338
Size: 1104 977 1905
Fragments arranged by size:
1905 1104 977
AgeI A'CCGG_T
Cuts at: 2374 2374
Size: 3986
AlwNI CAG_nnn'CTG
Cuts at: 747 1291 2858 747
Size: 544 1567 1875
Fragments arranged by size:
1875 1567 544
ApaLI G'TGCA_C
Cuts at: 2756 2756
Size: 3986
ApoI r'AATT_y
Cuts at: 990 3339 3595 990
Size: 2349 256 1381
Fragments arranged by size:
2349 1381 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3986
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3986
AvaI C'yCGr_G
Cuts at: 219 1006 219
Size: 787 3199
BamHI G'GATC_C
Cuts at: 1011 1011
Size: 3986
BanI G'GyrC_C
Cuts at: 885 1002 1416 1664 1699 885
Size: 117 414 248 35 3172
Fragments arranged by size:
3172 414 248 117 35
BanII G_rGCy'C
Cuts at: 953 1000 2030 953
Size: 47 1030 2909
Fragments arranged by size:
2909 1030 47
BbeI G_GCGC'C
Cuts at: 889 1420 1668 889
Size: 531 248 3207
Fragments arranged by size:
3207 531 248
BbsI GAAGACnn'nnnn_
Cuts at: 932 932
Size: 3986
BbvCI CC'TCA_GC
Cuts at: 563 908 563
Size: 345 3641
BciVI GTATCCnnnnn_n'
Cuts at: 1884 2645 3986 1884
Size: 761 1341 1884
Fragments arranged by size:
1884 1341 761
BclI T'GATC_A
Cuts at: 1506 1506
Size: 3986
BglII A'GATC_T
Cuts at: 1501 3538 1501
Size: 2037 1949
BlpI GC'TnA_GC
Cuts at: 535 1098 535
Size: 563 3423
Bme1580I G_kGCm'C
Cuts at: 133 170 1611 1704 2760 133
Size: 37 1441 93 1056 1359
Fragments arranged by size:
1441 1359 1056 93 37
BmgBI CAC'GTC
Cuts at: 978 978
Size: 3986
BmrI ACTGGGnnnn_n'
Cuts at: 1198 1279 1614 3758 1198
Size: 81 335 2144 1426
Fragments arranged by size:
2144 1426 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 3986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 844
Size: 3986
Bpu10I CC'TnA_GC
Cuts at: 283 563 908 283
Size: 280 345 3361
Fragments arranged by size:
3361 345 280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1162 2041 2533 2831 1162
Size: 879 492 298 2317
Fragments arranged by size:
2317 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3690 3690
Size: 3986
BsaAI yAC'GTr
Cuts at: 858 1339 858
Size: 481 3505
BsaBI GATnn'nnATC
Cuts at: 6 1500 3537 6
Size: 1494 2037 455
Fragments arranged by size:
2037 1494 455
BsaHI Gr'CG_yC
Cuts at: 421 886 1417 1665 2367 3863 421
Size: 465 531 248 702 1496 544
Fragments arranged by size:
1496 702 544 531 465 248
BsaWI w'CCGG_w
Cuts at: 1175 1696 2374 2648 2795 3206 1175
Size: 521 678 274 147 411 1955
Fragments arranged by size:
1955 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 346 376 346
Size: 30 3956
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 351 580 841 877 946 949 952 955 958
Size: 229 261 36 69 3 3 3 3
Cuts at: 958 961 351
Size: 3 3376
Fragments arranged by size:
3376 261 229 69 36 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 149 463 1411 2169 2746 3242 149
Size: 314 948 758 577 496 893
Fragments arranged by size:
948 893 758 577 496 314
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 606 646 823 606
Size: 40 177 3769
Fragments arranged by size:
3769 177 40
BsiEI CG_ry'CG
Cuts at: 104 191 701 1528 1574 2782 104
Size: 87 510 827 46 1208 1308
Fragments arranged by size:
1308 1208 827 510 87 46
BsiHKAI G_wGCw'C
Cuts at: 289 977 1000 1778 1968 2760 289
Size: 688 23 778 190 792 1515
Fragments arranged by size:
1515 792 778 688 190 23
BsmBI CGTCTCn'nnnn_
Cuts at: 625 625
Size: 3986
Bsp1286I G_dGCh'C
Cuts at: 133 170 289 953 977 1000 1611 1704 1778
Size: 37 119 664 24 23 611 93 74
Cuts at: 1778 1968 2030 2760 133
Size: 190 62 730 1359
Fragments arranged by size:
1359 730 664 611 190 119 93 74
62 37 24 23
BspEI T'CCGG_A
Cuts at: 3206 3206
Size: 3986
BspHI T'CATG_A
Cuts at: 3162 3162
Size: 3986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 798 1036 1250 1552 1933 798
Size: 238 214 302 381 2851
Fragments arranged by size:
2851 381 302 238 214
BsrBI CCG'CTC
Cuts at: 50 114 224 539 2278 2332 3816 3981 50
Size: 64 110 315 1739 54 1484 165 55
Fragments arranged by size:
1739 1484 315 165 110 64 55 54
BsrDI GCAATG_nn'
Cuts at: 557 1898 557
Size: 1341 2645
BsrFI r'CCGG_y
Cuts at: 190 438 1984 2165 2374 190
Size: 248 1546 181 209 1802
Fragments arranged by size:
1802 1546 248 209 181
BssHII G'CGCG_C
Cuts at: 256 256
Size: 3986
BssSI C'ACGA_G
Cuts at: 2257 2615 2257
Size: 358 3628
BstAPI GCAn_nnn'nTGC
Cuts at: 810 1363 810
Size: 553 3433
BstBI TT'CG_AA
Cuts at: 2347 2347
Size: 3986
BstEII G'GTnAC_C
Cuts at: 441 765 441
Size: 324 3662
BstYI r'GATC_y
Cuts at: 1 1011 1478 1501 1836 2082 3094 3180 3192
Size: 1010 467 23 335 246 1012 86 12
Cuts at: 3192 3538 1
Size: 346 449
Fragments arranged by size:
1012 1010 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 275 581 704 107
Size: 168 306 123 3389
Fragments arranged by size:
3389 306 168 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 633 1936 2117 2280 633
Size: 1303 181 163 2339
Fragments arranged by size:
2339 1303 181 163
BtsI GCAGTG_nn'
Cuts at: 1229 1229
Size: 3986
DraI TTT'AAA
Cuts at: 985 985
Size: 3986
DrdI GACnn_nn'nnGTC
Cuts at: 639 1692 2550 639
Size: 1053 858 2075
Fragments arranged by size:
2075 1053 858
EaeI y'GGCC_r
Cuts at: 188 192 644 698 839 1571 1745 2136 2163
Size: 4 452 54 141 732 174 391 27
Cuts at: 2163 3508 188
Size: 1345 666
Fragments arranged by size:
1345 732 666 452 391 174 141 54
27 4
EagI C'GGCC_G
Cuts at: 188 698 1571 188
Size: 510 873 2603
Fragments arranged by size:
2603 873 510
EarI CTCTTCn'nnn_
Cuts at: 112 523 788 2009 2219 112
Size: 411 265 1221 210 1879
Fragments arranged by size:
1879 1221 411 265 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2194 2504 2650 3796 2194
Size: 310 146 1146 2384
Fragments arranged by size:
2384 1146 310 146
EcoICRI GAG'CTC
Cuts at: 998 998
Size: 3986
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1811 2243 2990 3258 844
Size: 967 432 747 268 1572
Fragments arranged by size:
1572 967 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 592 592
Size: 3986
EcoO109I rG'GnC_Cy
Cuts at: 597 613 1125 597
Size: 16 512 3458
Fragments arranged by size:
3458 512 16
EcoRI G'AATT_C
Cuts at: 990 990
Size: 3986
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2005 2005
Size: 3986
FseI GG_CCGG'CC
Cuts at: 194 194
Size: 3986
FspI TGC'GCA
Cuts at: 1767 1767
Size: 3986
HaeII r_GCGC'y
Cuts at: 139 403 659 835 889 1420 1668 2690 3669
Size: 264 256 176 54 531 248 1022 979
Cuts at: 3669 139
Size: 456
Fragments arranged by size:
1022 979 531 456 264 256 248 176
54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1828 1858 1860 1890 1828
Size: 30 2 30 3924
Fragments arranged by size:
3924 30 30 2
HincII GTy'rAC
Cuts at: 1025 1025
Size: 3986
KasI G'GCGC_C
Cuts at: 885 1416 1664 885
Size: 531 248 3207
Fragments arranged by size:
3207 531 248
KpnI G_GTAC'C
Cuts at: 1006 1006
Size: 3986
MluI A'CGCG_T
Cuts at: 3419 3419
Size: 3986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 389 2657 2841 389
Size: 2268 184 1534
Fragments arranged by size:
2268 1534 184
MscI TGG'CCA
Cuts at: 1747 1747
Size: 3986
MslI CAynn'nnrTG
Cuts at: 200 1975 2102 200
Size: 1775 127 2084
Fragments arranged by size:
2084 1775 127
MspA1I CmG'CkG
Cuts at: 252 891 1097 1411 1771 2784 3029 252
Size: 639 206 314 360 1013 245 1209
Fragments arranged by size:
1209 1013 639 360 314 245 206
NaeI GCC'GGC
Cuts at: 192 2167 192
Size: 1975 2011
NarI GG'CG_CC
Cuts at: 886 1417 1665 886
Size: 531 248 3207
Fragments arranged by size:
3207 531 248
NcoI C'CATG_G
Cuts at: 107 275 581 107
Size: 168 306 3512
Fragments arranged by size:
3512 306 168
NgoMIV G'CCGG_C
Cuts at: 190 2165 190
Size: 1975 2011
NspI r_CATG'y
Cuts at: 849 1039 1539 2446 849
Size: 190 500 907 2389
Fragments arranged by size:
2389 907 500 190
PciI A'CATG_T
Cuts at: 2442 2442
Size: 3986
PmeI GTTT'AAAC
Cuts at: 985 985
Size: 3986
PmlI CAC'GTG
Cuts at: 858 858
Size: 3986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3125 3157 3125
Size: 32 3954
PpuMI rG'GwC_Cy
Cuts at: 613 613
Size: 3986
PstI C_TGCA'G
Cuts at: 605 1033 1718 605
Size: 428 685 2873
Fragments arranged by size:
2873 685 428
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3986
PvuII CAG'CTG
Cuts at: 252 1411 1771 252
Size: 1159 360 2467
Fragments arranged by size:
2467 1159 360
RsrII CG'GwC_CG
Cuts at: 2181 2181
Size: 3986
SacI G_AGCT'C
Cuts at: 1000 1000
Size: 3986
SalI G'TCGA_C
Cuts at: 1023 1023
Size: 3986
SapI GCTCTTCn'nnn_
Cuts at: 523 788 2009 2219 523
Size: 265 1221 210 2290
Fragments arranged by size:
2290 1221 265 210
SbfI CC_TGCA'GG
Cuts at: 605 1033 605
Size: 428 3558
ScaI AGT'ACT
Cuts at: 3503 3503
Size: 3986
SexAI A'CCwGG_T
Cuts at: 368 368
Size: 3986
SfcI C'TryA_G
Cuts at: 33 213 601 1029 1714 2707 2898 33
Size: 180 388 428 685 993 191 1121
Fragments arranged by size:
1121 993 685 428 388 191 180
SfoI GGC'GCC
Cuts at: 887 1418 1666 887
Size: 531 248 3207
Fragments arranged by size:
3207 531 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3986
SmaI CCC'GGG
Cuts at: 1008 1008
Size: 3986
SmlI C'TyrA_G
Cuts at: 1141 2056 2548 2810 1141
Size: 915 492 262 2317
Fragments arranged by size:
2317 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3986
SphI G_CATG'C
Cuts at: 1039 1539 1039
Size: 500 3486
StuI AGG'CCT
Cuts at: 901 901
Size: 3986
StyI C'CwwG_G
Cuts at: 107 275 581 593 1120 1474 107
Size: 168 306 12 527 354 2619
Fragments arranged by size:
2619 527 354 306 168 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 784 1569 1702 2161 2370 784
Size: 785 133 459 209 2400
Fragments arranged by size:
2400 785 459 209 133
TatI w'GTAC_w
Cuts at: 3501 3501
Size: 3986
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3503 3535 3503
Size: 32 3954
Tth111I GACn'n_nGTC
Cuts at: 420 1783 420
Size: 1363 2623
XbaI T'CTAG_A
Cuts at: 1017 1017
Size: 3986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1481 1481
Size: 3986
XmaI C'CCGG_G
Cuts at: 1006 1006
Size: 3986
XmnI GAAnn'nnTTC
Cuts at: 232 550 232
Size: 318 3668
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmtI BsiWI
BsmI BsrGI BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI