Restriction map for
pF1KB6338
(Circular) (Six-base) MAPSORT of: pF1KB6338.seq Check: 8900 from: 1 to: 3983
pF1KB6338 3983 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 458 1021 458
Size: 563 3420
Acc65I G'GTAC_C
Cuts at: 833 999 833
Size: 166 3817
AclI AA'CG_TT
Cuts at: 3823 3823
Size: 3983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 296 370 746 1808 2240 2987 3255 296
Size: 74 376 1062 432 747 268 1024
Fragments arranged by size:
1062 1024 747 432 376 268 74
AflIII A'CryG_T
Cuts at: 1335 2439 3416 1335
Size: 1104 977 1902
Fragments arranged by size:
1902 1104 977
AgeI A'CCGG_T
Cuts at: 2371 2371
Size: 3983
AleI CACnn'nnGTG
Cuts at: 204 204
Size: 3983
AlwNI CAG_nnn'CTG
Cuts at: 769 1288 2855 769
Size: 519 1567 1897
Fragments arranged by size:
1897 1567 519
ApaLI G'TGCA_C
Cuts at: 2753 2753
Size: 3983
ApoI r'AATT_y
Cuts at: 946 987 3336 3592 946
Size: 41 2349 256 1337
Fragments arranged by size:
2349 1337 256 41
AseI AT'TA_AT
Cuts at: 20 20
Size: 3983
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
AvaI C'yCGr_G
Cuts at: 328 429 1003 328
Size: 101 574 3308
Fragments arranged by size:
3308 574 101
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 817 850 817
Size: 33 3950
BamHI G'GATC_C
Cuts at: 1008 1008
Size: 3983
BanI G'GyrC_C
Cuts at: 479 833 999 1413 1661 1696 479
Size: 354 166 414 248 35 2766
Fragments arranged by size:
2766 414 354 248 166 35
BanII G_rGCy'C
Cuts at: 176 997 2027 176
Size: 821 1030 2132
Fragments arranged by size:
2132 1030 821
BbeI G_GCGC'C
Cuts at: 1417 1665 1417
Size: 248 3735
BbsI GAAGACnn'nnnn_
Cuts at: 584 752 910 584
Size: 168 158 3657
Fragments arranged by size:
3657 168 158
BciVI GTATCCnnnnn_n'
Cuts at: 1881 2642 3983 1881
Size: 761 1341 1881
Fragments arranged by size:
1881 1341 761
BclI T'GATC_A
Cuts at: 1503 1503
Size: 3983
BglII A'GATC_T
Cuts at: 279 1498 3535 279
Size: 1219 2037 727
Fragments arranged by size:
2037 1219 727
BlpI GC'TnA_GC
Cuts at: 1095 1095
Size: 3983
Bme1580I G_kGCm'C
Cuts at: 1608 1701 2757 1608
Size: 93 1056 2834
Fragments arranged by size:
2834 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1195 1276 1611 3755 1195
Size: 81 335 2144 1423
Fragments arranged by size:
2144 1423 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 3983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 325 532 187
Size: 138 207 3638
Fragments arranged by size:
3638 207 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 402 773 953 1159 2038 2530 2828 402
Size: 371 180 206 879 492 298 1557
Fragments arranged by size:
1557 879 492 371 298 206 180
BsaI GGTCTCn'nnnn_
Cuts at: 3687 3687
Size: 3983
BsaAI yAC'GTr
Cuts at: 1336 1336
Size: 3983
BsaBI GATnn'nnATC
Cuts at: 6 1497 3534 6
Size: 1491 2037 455
Fragments arranged by size:
2037 1491 455
BsaHI Gr'CG_yC
Cuts at: 1414 1662 2364 3860 1414
Size: 248 702 1496 1537
Fragments arranged by size:
1537 1496 702 248
BsaWI w'CCGG_w
Cuts at: 176 1172 1693 2371 2645 2792 3203 176
Size: 996 521 678 274 147 411 956
Fragments arranged by size:
996 956 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 703 733 703
Size: 30 3953
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 220
Size: 3983
BseYI C'CCAG_C
Cuts at: 121 1408 2166 2743 3239 121
Size: 1287 758 577 496 865
Fragments arranged by size:
1287 865 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 535
Size: 3983
BsiEI CG_ry'CG
Cuts at: 104 1525 1571 2779 104
Size: 1421 46 1208 1308
Fragments arranged by size:
1421 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 329 997 1775 1965 2757 329
Size: 668 778 190 792 1555
Fragments arranged by size:
1555 792 778 668 190
Bsp1286I G_dGCh'C
Cuts at: 176 329 997 1608 1701 1775 1965 2027 2757
Size: 153 668 611 93 74 190 62 730
Cuts at: 2757 176
Size: 1402
Fragments arranged by size:
1402 730 668 611 190 153 93 74
62
BspEI T'CCGG_A
Cuts at: 176 3203 176
Size: 3027 956
BspHI T'CATG_A
Cuts at: 3159 3159
Size: 3983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1033 1247 1549 1930 1033
Size: 214 302 381 3086
Fragments arranged by size:
3086 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2275 2329 3813 3978 50
Size: 2225 54 1484 165 55
Fragments arranged by size:
2225 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1895 1895
Size: 3983
BsrFI r'CCGG_y
Cuts at: 1981 2162 2371 1981
Size: 181 209 3593
Fragments arranged by size:
3593 209 181
BssSI C'ACGA_G
Cuts at: 2254 2612 2254
Size: 358 3625
BstAPI GCAn_nnn'nTGC
Cuts at: 1360 1360
Size: 3983
BstBI TT'CG_AA
Cuts at: 915 2344 915
Size: 1429 2554
BstYI r'GATC_y
Cuts at: 1 279 1008 1475 1498 1833 2079 3091 3177
Size: 278 729 467 23 335 246 1012 86
Cuts at: 3177 3189 3535 1
Size: 12 346 449
Fragments arranged by size:
1012 729 467 449 346 335 278 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 374 592 374
Size: 218 3765
BtgI C'CryG_G
Cuts at: 107 107
Size: 3983
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1933 2114 2277 1933
Size: 181 163 3639
Fragments arranged by size:
3639 181 163
BtsI GCAGTG_nn'
Cuts at: 614 806 881 1226 614
Size: 192 75 345 3371
Fragments arranged by size:
3371 345 192 75
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 275 310 275
Size: 35 3948
DraI TTT'AAA
Cuts at: 951 982 951
Size: 31 3952
DraIII CAC_nnn'GTG
Cuts at: 392 392
Size: 3983
DrdI GACnn_nn'nnGTC
Cuts at: 1689 2547 1689
Size: 858 3125
EaeI y'GGCC_r
Cuts at: 1568 1742 2133 2160 3505 1568
Size: 174 391 27 1345 2046
Fragments arranged by size:
2046 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1568 1568
Size: 3983
EarI CTCTTCn'nnn_
Cuts at: 2006 2216 2006
Size: 210 3773
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2191 2501 2647 3793 2191
Size: 310 146 1146 2381
Fragments arranged by size:
2381 1146 310 146
EcoICRI GAG'CTC
Cuts at: 995 995
Size: 3983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 296 325 370 532 746 1808 2240 2987
Size: 109 29 45 162 214 1062 432 747
Cuts at: 2987 3255 187
Size: 268 915
Fragments arranged by size:
1062 915 747 432 268 214 162 109
45 29
EcoO109I rG'GnC_Cy
Cuts at: 552 963 1122 552
Size: 411 159 3413
Fragments arranged by size:
3413 411 159
EcoRI G'AATT_C
Cuts at: 987 987
Size: 3983
EcoRV GAT'ATC
Cuts at: 182 182
Size: 3983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2002 2002
Size: 3983
FspI TGC'GCA
Cuts at: 1764 1764
Size: 3983
HaeII r_GCGC'y
Cuts at: 1417 1665 2687 3666 1417
Size: 248 1022 979 1734
Fragments arranged by size:
1734 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 264 296 703 735 1825 1855 1857 1887 264
Size: 32 407 32 1090 30 2 30 2360
Fragments arranged by size:
2360 1090 407 32 32 30 30 2
HincII GTy'rAC
Cuts at: 459 806 1022 459
Size: 347 216 3420
Fragments arranged by size:
3420 347 216
KasI G'GCGC_C
Cuts at: 1413 1661 1413
Size: 248 3735
KpnI G_GTAC'C
Cuts at: 837 1003 837
Size: 166 3817
MluI A'CGCG_T
Cuts at: 3416 3416
Size: 3983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2654 2838 2654
Size: 184 3799
MscI TGG'CCA
Cuts at: 1744 1744
Size: 3983
MslI CAynn'nnrTG
Cuts at: 204 1972 2099 204
Size: 1768 127 2088
Fragments arranged by size:
2088 1768 127
MspA1I CmG'CkG
Cuts at: 225 262 605 716 1094 1408 1768 2781 3026
Size: 37 343 111 378 314 360 1013 245
Cuts at: 3026 225
Size: 1182
Fragments arranged by size:
1182 1013 378 360 343 314 245 111
37
NaeI GCC'GGC
Cuts at: 2164 2164
Size: 3983
NarI GG'CG_CC
Cuts at: 1414 1662 1414
Size: 248 3735
NcoI C'CATG_G
Cuts at: 107 107
Size: 3983
NgoMIV G'CCGG_C
Cuts at: 2162 2162
Size: 3983
NspI r_CATG'y
Cuts at: 270 1036 1536 2443 270
Size: 766 500 907 1810
Fragments arranged by size:
1810 907 766 500
PciI A'CATG_T
Cuts at: 2439 2439
Size: 3983
PflMI CCAn_nnn'nTGG
Cuts at: 392 392
Size: 3983
PfoI T'CCnGG_A
Cuts at: 302 302
Size: 3983
PmeI GTTT'AAAC
Cuts at: 982 982
Size: 3983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3122 3154 3122
Size: 32 3951
PpuMI rG'GwC_Cy
Cuts at: 963 963
Size: 3983
PspXI vC'TCGA_Gb
Cuts at: 429 429
Size: 3983
PstI C_TGCA'G
Cuts at: 733 1030 1715 733
Size: 297 685 3001
Fragments arranged by size:
3001 685 297
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3983
PvuII CAG'CTG
Cuts at: 225 262 605 1408 1768 225
Size: 37 343 803 360 2440
Fragments arranged by size:
2440 803 360 343 37
RsrII CG'GwC_CG
Cuts at: 2178 2178
Size: 3983
SacI G_AGCT'C
Cuts at: 997 997
Size: 3983
SalI G'TCGA_C
Cuts at: 457 1020 457
Size: 563 3420
SapI GCTCTTCn'nnn_
Cuts at: 2006 2216 2006
Size: 210 3773
SbfI CC_TGCA'GG
Cuts at: 1030 1030
Size: 3983
ScaI AGT'ACT
Cuts at: 3500 3500
Size: 3983
SfcI C'TryA_G
Cuts at: 33 729 1026 1711 2704 2895 33
Size: 696 297 685 993 191 1121
Fragments arranged by size:
1121 993 696 685 297 191
SfoI GGC'GCC
Cuts at: 1415 1663 1415
Size: 248 3735
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
SmaI CCC'GGG
Cuts at: 1005 1005
Size: 3983
SmlI C'TyrA_G
Cuts at: 328 417 429 788 968 1138 2053 2545 2807
Size: 89 12 359 180 170 915 492 262
Cuts at: 2807 328
Size: 1504
Fragments arranged by size:
1504 915 492 359 262 180 170 89
12
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3983
SphI G_CATG'C
Cuts at: 270 1036 1536 270
Size: 766 500 2717
Fragments arranged by size:
2717 766 500
StuI AGG'CCT
Cuts at: 427 427
Size: 3983
StyI C'CwwG_G
Cuts at: 107 1117 1471 107
Size: 1010 354 2619
Fragments arranged by size:
2619 1010 354
SwaI ATTT'AAAT
Cuts at: 951 951
Size: 3983
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 552 1566 1699 2158 2367 100
Size: 452 1014 133 459 209 1716
Fragments arranged by size:
1716 1014 459 452 209 133
TatI w'GTAC_w
Cuts at: 473 3498 473
Size: 3025 958
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3500 3532 3500
Size: 32 3951
Tth111I GACn'n_nGTC
Cuts at: 1780 1780
Size: 3983
XbaI T'CTAG_A
Cuts at: 1014 1014
Size: 3983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1478 1478
Size: 3983
XhoI C'TCGA_G
Cuts at: 328 429 328
Size: 101 3882
XmaI C'CCGG_G
Cuts at: 1003 1003
Size: 3983
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspOMI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI XmnI ZraI