Restriction map for
pF1KB6335
(Circular) (Six-base) MAPSORT of: pF1KB6335.seq Check: 5076 from: 1 to: 3896
pF1KB6335 3896 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 934 934
Size: 3896
Acc65I G'GTAC_C
Cuts at: 737 912 737
Size: 175 3721
AclI AA'CG_TT
Cuts at: 3736 3736
Size: 3896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1721 2153 2900 3168 1721
Size: 432 747 268 2449
Fragments arranged by size:
2449 747 432 268
AflIII A'CryG_T
Cuts at: 1248 2352 3329 1248
Size: 1104 977 1815
Fragments arranged by size:
1815 1104 977
AgeI A'CCGG_T
Cuts at: 2284 2284
Size: 3896
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 564 564
Size: 3896
AlwNI CAG_nnn'CTG
Cuts at: 1201 2768 1201
Size: 1567 2329
ApaLI G'TGCA_C
Cuts at: 2666 2666
Size: 3896
ApoI r'AATT_y
Cuts at: 488 535 610 900 3249 3505 488
Size: 47 75 290 2349 256 879
Fragments arranged by size:
2349 879 290 256 75 47
AscI GG'CGCG_CC
Cuts at: 118 118
Size: 3896
AseI AT'TA_AT
Cuts at: 20 20
Size: 3896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
AvaI C'yCGr_G
Cuts at: 916 916
Size: 3896
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 721 754 721
Size: 33 3863
BamHI G'GATC_C
Cuts at: 921 921
Size: 3896
BanI G'GyrC_C
Cuts at: 737 912 1326 1574 1609 737
Size: 175 414 248 35 3024
Fragments arranged by size:
3024 414 248 175 35
BanII G_rGCy'C
Cuts at: 136 910 1940 136
Size: 774 1030 2092
Fragments arranged by size:
2092 1030 774
BbeI G_GCGC'C
Cuts at: 1330 1578 1330
Size: 248 3648
BciVI GTATCCnnnnn_n'
Cuts at: 390 1794 2555 3896 390
Size: 1404 761 1341 390
Fragments arranged by size:
1404 1341 761 390
BclI T'GATC_A
Cuts at: 1416 1416
Size: 3896
BglII A'GATC_T
Cuts at: 555 1411 3448 555
Size: 856 2037 1003
Fragments arranged by size:
2037 1003 856
BlpI GC'TnA_GC
Cuts at: 1008 1008
Size: 3896
Bme1580I G_kGCm'C
Cuts at: 1521 1614 2670 1521
Size: 93 1056 2747
Fragments arranged by size:
2747 1056 93
BmgBI CAC'GTC
Cuts at: 240 240
Size: 3896
BmrI ACTGGGnnnn_n'
Cuts at: 1108 1189 1524 3668 1108
Size: 81 335 2144 1336
Fragments arranged by size:
2144 1336 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 3896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 186
Size: 3896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 148 1072 1951 2443 2741 127
Size: 21 924 879 492 298 1282
Fragments arranged by size:
1282 924 879 492 298 21
BsaI GGTCTCn'nnnn_
Cuts at: 3600 3600
Size: 3896
BsaAI yAC'GTr
Cuts at: 544 1249 544
Size: 705 3191
BsaBI GATnn'nnATC
Cuts at: 6 1410 3447 6
Size: 1404 2037 455
Fragments arranged by size:
2037 1404 455
BsaHI Gr'CG_yC
Cuts at: 1327 1575 2277 3773 1327
Size: 248 702 1496 1450
Fragments arranged by size:
1496 1450 702 248
BsaWI w'CCGG_w
Cuts at: 1085 1606 2284 2558 2705 3116 1085
Size: 521 678 274 147 411 1865
Fragments arranged by size:
1865 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 563 593 563
Size: 30 3866
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 694 694
Size: 3896
BseYI C'CCAG_C
Cuts at: 188 1321 2079 2656 3152 188
Size: 1133 758 577 496 932
Fragments arranged by size:
1133 932 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 271
Size: 3896
BsiEI CG_ry'CG
Cuts at: 104 297 1438 1484 2692 104
Size: 193 1141 46 1208 1308
Fragments arranged by size:
1308 1208 1141 193 46
BsiHKAI G_wGCw'C
Cuts at: 910 1688 1878 2670 910
Size: 778 190 792 2136
Fragments arranged by size:
2136 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 246 246
Size: 3896
Bsp1286I G_dGCh'C
Cuts at: 136 910 1521 1614 1688 1878 1940 2670 136
Size: 774 611 93 74 190 62 730 1362
Fragments arranged by size:
1362 774 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3116 3116
Size: 3896
BspHI T'CATG_A
Cuts at: 3072 3072
Size: 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 224 946 1160 1462 1843 224
Size: 722 214 302 381 2277
Fragments arranged by size:
2277 722 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2188 2242 3726 3891 50
Size: 2138 54 1484 165 55
Fragments arranged by size:
2138 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 363 1808 363
Size: 1445 2451
BsrFI r'CCGG_y
Cuts at: 352 1894 2075 2284 352
Size: 1542 181 209 1964
Fragments arranged by size:
1964 1542 209 181
BssHII G'CGCG_C
Cuts at: 118 118
Size: 3896
BssSI C'ACGA_G
Cuts at: 2167 2525 2167
Size: 358 3538
BstAPI GCAn_nnn'nTGC
Cuts at: 1273 1273
Size: 3896
BstBI TT'CG_AA
Cuts at: 2257 2257
Size: 3896
BstYI r'GATC_y
Cuts at: 1 555 886 921 1388 1411 1746 1992 3004
Size: 554 331 35 467 23 335 246 1012
Cuts at: 3004 3090 3102 3448 1
Size: 86 12 346 449
Fragments arranged by size:
1012 554 467 449 346 335 331 246
86 35 23 12
BtgI C'CryG_G
Cuts at: 308 308
Size: 3896
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1846 2027 2190 1846
Size: 181 163 3552
Fragments arranged by size:
3552 181 163
BtsI GCAGTG_nn'
Cuts at: 1139 1139
Size: 3896
DraI TTT'AAA
Cuts at: 895 895
Size: 3896
DrdI GACnn_nn'nnGTC
Cuts at: 1602 2460 1602
Size: 858 3038
EaeI y'GGCC_r
Cuts at: 305 1481 1655 2046 2073 3418 305
Size: 1176 174 391 27 1345 783
Fragments arranged by size:
1345 1176 783 391 174 27
EagI C'GGCC_G
Cuts at: 1481 1481
Size: 3896
EarI CTCTTCn'nnn_
Cuts at: 777 1919 2129 777
Size: 1142 210 2544
Fragments arranged by size:
2544 1142 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2104 2414 2560 3706 2104
Size: 310 146 1146 2294
Fragments arranged by size:
2294 1146 310 146
EcoICRI GAG'CTC
Cuts at: 908 908
Size: 3896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 1721 2153 2900 3168 186
Size: 1535 432 747 268 914
Fragments arranged by size:
1535 914 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 127 145 127
Size: 18 3878
EcoO109I rG'GnC_Cy
Cuts at: 168 1035 168
Size: 867 3029
EcoRI G'AATT_C
Cuts at: 488 900 488
Size: 412 3484
EcoRV GAT'ATC
Cuts at: 366 366
Size: 3896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1915 1915
Size: 3896
FspI TGC'GCA
Cuts at: 1677 1677
Size: 3896
HaeII r_GCGC'y
Cuts at: 409 1330 1578 2600 3579 409
Size: 921 248 1022 979 726
Fragments arranged by size:
1022 979 921 726 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 1738 1768 1770 1800 184
Size: 32 1522 30 2 30 2280
Fragments arranged by size:
2280 1522 32 30 30 2
HincII GTy'rAC
Cuts at: 935 935
Size: 3896
KasI G'GCGC_C
Cuts at: 1326 1574 1326
Size: 248 3648
KpnI G_GTAC'C
Cuts at: 741 916 741
Size: 175 3721
MluI A'CGCG_T
Cuts at: 3329 3329
Size: 3896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 134 2567 2751 134
Size: 2433 184 1279
Fragments arranged by size:
2433 1279 184
MscI TGG'CCA
Cuts at: 307 1657 307
Size: 1350 2546
MslI CAynn'nnrTG
Cuts at: 313 328 546 700 1885 2012 313
Size: 15 218 154 1185 127 2197
Fragments arranged by size:
2197 1185 218 154 127 15
MspA1I CmG'CkG
Cuts at: 206 290 509 1007 1321 1681 2694 2939 206
Size: 84 219 498 314 360 1013 245 1163
Fragments arranged by size:
1163 1013 498 360 314 245 219 84
NaeI GCC'GGC
Cuts at: 354 2077 354
Size: 1723 2173
NarI GG'CG_CC
Cuts at: 1327 1575 1327
Size: 248 3648
NcoI C'CATG_G
Cuts at: 308 308
Size: 3896
NgoMIV G'CCGG_C
Cuts at: 352 2075 352
Size: 1723 2173
NspI r_CATG'y
Cuts at: 949 1449 2356 949
Size: 500 907 2489
Fragments arranged by size:
2489 907 500
PciI A'CATG_T
Cuts at: 2352 2352
Size: 3896
PmeI GTTT'AAAC
Cuts at: 895 895
Size: 3896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 3864
PpuMI rG'GwC_Cy
Cuts at: 168 168
Size: 3896
PstI C_TGCA'G
Cuts at: 221 801 943 1628 221
Size: 580 142 685 2489
Fragments arranged by size:
2489 685 580 142
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3896
PvuII CAG'CTG
Cuts at: 509 1321 1681 509
Size: 812 360 2724
Fragments arranged by size:
2724 812 360
RsrII CG'GwC_CG
Cuts at: 2091 2091
Size: 3896
SacI G_AGCT'C
Cuts at: 910 910
Size: 3896
SalI G'TCGA_C
Cuts at: 933 933
Size: 3896
SapI GCTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
SbfI CC_TGCA'GG
Cuts at: 943 943
Size: 3896
ScaI AGT'ACT
Cuts at: 3413 3413
Size: 3896
SfcI C'TryA_G
Cuts at: 33 217 797 939 1624 2617 2808 33
Size: 184 580 142 685 993 191 1121
Fragments arranged by size:
1121 993 685 580 191 184 142
SfoI GGC'GCC
Cuts at: 1328 1576 1328
Size: 248 3648
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
SmaI CCC'GGG
Cuts at: 918 918
Size: 3896
SmlI C'TyrA_G
Cuts at: 142 163 1051 1966 2458 2720 142
Size: 21 888 915 492 262 1318
Fragments arranged by size:
1318 915 888 492 262 21
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3896
SphI G_CATG'C
Cuts at: 949 1449 949
Size: 500 3396
StuI AGG'CCT
Cuts at: 184 184
Size: 3896
StyI C'CwwG_G
Cuts at: 308 401 1030 1384 308
Size: 93 629 354 2820
Fragments arranged by size:
2820 629 354 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1479 1612 2071 2280 1479
Size: 133 459 209 3095
Fragments arranged by size:
3095 459 209 133
TatI w'GTAC_w
Cuts at: 503 3411 503
Size: 2908 988
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 3864
Tth111I GACn'n_nGTC
Cuts at: 1693 1693
Size: 3896
XbaI T'CTAG_A
Cuts at: 927 927
Size: 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1391 1391
Size: 3896
XmaI C'CCGG_G
Cuts at: 916 916
Size: 3896
XmnI GAAnn'nnTTC
Cuts at: 713 713
Size: 3896
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AvrII BbsI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsiWI BsmI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI