Restriction map for
pF1KB6333
(Circular) (Six-base) MAPSORT of: pF1KB6333.seq Check: 3151 from: 1 to: 3896
pF1KB6333 3896 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 213 568 739 213
Size: 355 171 3370
Fragments arranged by size:
3370 355 171
AccI GT'mk_AC
Cuts at: 934 934
Size: 3896
Acc65I G'GTAC_C
Cuts at: 912 912
Size: 3896
AclI AA'CG_TT
Cuts at: 3736 3736
Size: 3896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 445 604 1721 2153 2900 3168 377
Size: 68 159 1117 432 747 268 1105
Fragments arranged by size:
1117 1105 747 432 268 159 68
AflIII A'CryG_T
Cuts at: 653 1248 2352 3329 653
Size: 595 1104 977 1220
Fragments arranged by size:
1220 1104 977 595
AgeI A'CCGG_T
Cuts at: 2284 2284
Size: 3896
AleI CACnn'nnGTG
Cuts at: 841 877 841
Size: 36 3860
AlwNI CAG_nnn'CTG
Cuts at: 493 1201 2768 493
Size: 708 1567 1621
Fragments arranged by size:
1621 1567 708
ApaI G_GGCC'C
Cuts at: 754 754
Size: 3896
ApaLI G'TGCA_C
Cuts at: 271 2666 271
Size: 2395 1501
ApoI r'AATT_y
Cuts at: 900 3249 3505 900
Size: 2349 256 1291
Fragments arranged by size:
2349 1291 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
AvaI C'yCGr_G
Cuts at: 735 916 735
Size: 181 3715
BamHI G'GATC_C
Cuts at: 921 921
Size: 3896
BanI G'GyrC_C
Cuts at: 912 1326 1574 1609 912
Size: 414 248 35 3199
Fragments arranged by size:
3199 414 248 35
BanII G_rGCy'C
Cuts at: 503 754 910 1940 503
Size: 251 156 1030 2459
Fragments arranged by size:
2459 1030 251 156
BbeI G_GCGC'C
Cuts at: 1330 1578 1330
Size: 248 3648
BbsI GAAGACnn'nnnn_
Cuts at: 117 365 117
Size: 248 3648
BciVI GTATCCnnnnn_n'
Cuts at: 1794 2555 3896 1794
Size: 761 1341 1794
Fragments arranged by size:
1794 1341 761
BclI T'GATC_A
Cuts at: 1416 1416
Size: 3896
BglI GCCn_nnn'nGGC
Cuts at: 822 822
Size: 3896
BglII A'GATC_T
Cuts at: 1411 3448 1411
Size: 2037 1859
BlpI GC'TnA_GC
Cuts at: 1008 1008
Size: 3896
Bme1580I G_kGCm'C
Cuts at: 275 754 813 1521 1614 2670 275
Size: 479 59 708 93 1056 1501
Fragments arranged by size:
1501 1056 708 479 93 59
BmgBI CAC'GTC
Cuts at: 146 654 788 146
Size: 508 134 3254
Fragments arranged by size:
3254 508 134
BmrI ACTGGGnnnn_n'
Cuts at: 273 559 1108 1189 1524 3668 273
Size: 286 549 81 335 2144 501
Fragments arranged by size:
2144 549 501 335 286 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 3896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 441 441
Size: 3896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1072 1951 2443 2741 1072
Size: 879 492 298 2227
Fragments arranged by size:
2227 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3600 3600
Size: 3896
BsaAI yAC'GTr
Cuts at: 1249 1249
Size: 3896
BsaBI GATnn'nnATC
Cuts at: 6 1410 3447 6
Size: 1404 2037 455
Fragments arranged by size:
2037 1404 455
BsaHI Gr'CG_yC
Cuts at: 1327 1575 2277 3773 1327
Size: 248 702 1496 1450
Fragments arranged by size:
1496 1450 702 248
BsaWI w'CCGG_w
Cuts at: 854 1085 1606 2284 2558 2705 3116 854
Size: 231 521 678 274 147 411 1634
Fragments arranged by size:
1634 678 521 411 274 231 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 610 610
Size: 3896
BseYI C'CCAG_C
Cuts at: 191 330 575 1321 2079 2656 3152 191
Size: 139 245 746 758 577 496 935
Fragments arranged by size:
935 758 746 577 496 245 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 143
Size: 3896
BsiEI CG_ry'CG
Cuts at: 104 1438 1484 2692 104
Size: 1334 46 1208 1308
Fragments arranged by size:
1334 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 275 503 910 1688 1878 2670 275
Size: 228 407 778 190 792 1501
Fragments arranged by size:
1501 792 778 407 228 190
BsmI GAATG_Cn'
Cuts at: 207 207
Size: 3896
Bsp1286I G_dGCh'C
Cuts at: 275 503 754 813 910 1521 1614 1688 1878
Size: 228 251 59 97 611 93 74 190
Cuts at: 1878 1940 2670 275
Size: 62 730 1501
Fragments arranged by size:
1501 730 611 251 228 190 97 93
74 62 59
BspEI T'CCGG_A
Cuts at: 3116 3116
Size: 3896
BspHI T'CATG_A
Cuts at: 3072 3072
Size: 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 213 536 568 739 946 1160 1462 1843 213
Size: 323 32 171 207 214 302 381 2266
Fragments arranged by size:
2266 381 323 302 214 207 171 32
BsrBI CCG'CTC
Cuts at: 50 405 2188 2242 3726 3891 50
Size: 355 1783 54 1484 165 55
Fragments arranged by size:
1783 1484 355 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1808 1808
Size: 3896
BsrFI r'CCGG_y
Cuts at: 1894 2075 2284 1894
Size: 181 209 3506
Fragments arranged by size:
3506 209 181
BsrGI T'GTAC_A
Cuts at: 833 833
Size: 3896
BssSI C'ACGA_G
Cuts at: 239 2167 2525 239
Size: 1928 358 1610
Fragments arranged by size:
1928 1610 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1273 1273
Size: 3896
BstBI TT'CG_AA
Cuts at: 2257 2257
Size: 3896
BstEII G'GTnAC_C
Cuts at: 681 681
Size: 3896
BstYI r'GATC_y
Cuts at: 1 921 1388 1411 1746 1992 3004 3090 3102
Size: 920 467 23 335 246 1012 86 12
Cuts at: 3102 3448 1
Size: 346 449
Fragments arranged by size:
1012 920 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 439 570 439
Size: 131 3765
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1846 2027 2190 1846
Size: 181 163 3552
Fragments arranged by size:
3552 181 163
BtsI GCAGTG_nn'
Cuts at: 300 804 1139 300
Size: 504 335 3057
Fragments arranged by size:
3057 504 335
ClaI AT'CG_AT
Cuts at: 432 432
Size: 3896
DraI TTT'AAA
Cuts at: 895 895
Size: 3896
DrdI GACnn_nn'nnGTC
Cuts at: 1602 2460 1602
Size: 858 3038
EaeI y'GGCC_r
Cuts at: 881 1481 1655 2046 2073 3418 881
Size: 600 174 391 27 1345 1359
Fragments arranged by size:
1359 1345 600 391 174 27
EagI C'GGCC_G
Cuts at: 1481 1481
Size: 3896
EarI CTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 469 2104 2414 2560 3706 469
Size: 1635 310 146 1146 659
Fragments arranged by size:
1635 1146 659 310 146
EcoICRI GAG'CTC
Cuts at: 501 908 501
Size: 407 3489
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 441 445 604 1721 2153 2900 3168 377
Size: 64 4 159 1117 432 747 268 1105
Fragments arranged by size:
1117 1105 747 432 268 159 64 4
EcoO109I rG'GnC_Cy
Cuts at: 114 285 750 1035 114
Size: 171 465 285 2975
Fragments arranged by size:
2975 465 285 171
EcoRI G'AATT_C
Cuts at: 900 900
Size: 3896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1915 1915
Size: 3896
FspI TGC'GCA
Cuts at: 662 1677 662
Size: 1015 2881
HaeII r_GCGC'y
Cuts at: 1330 1578 2600 3579 1330
Size: 248 1022 979 1647
Fragments arranged by size:
1647 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1738 1768 1770 1800 1738
Size: 30 2 30 3834
Fragments arranged by size:
3834 30 30 2
HincII GTy'rAC
Cuts at: 935 935
Size: 3896
KasI G'GCGC_C
Cuts at: 1326 1574 1326
Size: 248 3648
KpnI G_GTAC'C
Cuts at: 916 916
Size: 3896
MluI A'CGCG_T
Cuts at: 3329 3329
Size: 3896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2751 2567
Size: 184 3712
MscI TGG'CCA
Cuts at: 883 1657 883
Size: 774 3122
MslI CAynn'nnrTG
Cuts at: 841 877 1885 2012 841
Size: 36 1008 127 2725
Fragments arranged by size:
2725 1008 127 36
MspA1I CmG'CkG
Cuts at: 295 1007 1321 1681 2694 2939 295
Size: 712 314 360 1013 245 1252
Fragments arranged by size:
1252 1013 712 360 314 245
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 3896
NarI GG'CG_CC
Cuts at: 1327 1575 1327
Size: 248 3648
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 3896
NspI r_CATG'y
Cuts at: 949 1449 2356 949
Size: 500 907 2489
Fragments arranged by size:
2489 907 500
PciI A'CATG_T
Cuts at: 2352 2352
Size: 3896
PmeI GTTT'AAAC
Cuts at: 895 895
Size: 3896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 3864
PpuMI rG'GwC_Cy
Cuts at: 114 285 114
Size: 171 3725
PspOMI G'GGCC_C
Cuts at: 750 750
Size: 3896
PstI C_TGCA'G
Cuts at: 943 1628 943
Size: 685 3211
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3896
PvuII CAG'CTG
Cuts at: 295 1321 1681 295
Size: 1026 360 2510
Fragments arranged by size:
2510 1026 360
RsrII CG'GwC_CG
Cuts at: 2091 2091
Size: 3896
SacI G_AGCT'C
Cuts at: 503 910 503
Size: 407 3489
SalI G'TCGA_C
Cuts at: 933 933
Size: 3896
SanDI GG'GwC_CC
Cuts at: 114 114
Size: 3896
SapI GCTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
SbfI CC_TGCA'GG
Cuts at: 943 943
Size: 3896
ScaI AGT'ACT
Cuts at: 3413 3413
Size: 3896
SfcI C'TryA_G
Cuts at: 33 939 1624 2617 2808 33
Size: 906 685 993 191 1121
Fragments arranged by size:
1121 993 906 685 191
SfoI GGC'GCC
Cuts at: 1328 1576 1328
Size: 248 3648
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
SmaI CCC'GGG
Cuts at: 918 918
Size: 3896
SmlI C'TyrA_G
Cuts at: 1051 1966 2458 2720 1051
Size: 915 492 262 2227
Fragments arranged by size:
2227 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3896
SphI G_CATG'C
Cuts at: 949 1449 949
Size: 500 3396
StuI AGG'CCT
Cuts at: 825 825
Size: 3896
StyI C'CwwG_G
Cuts at: 839 1030 1384 839
Size: 191 354 3351
Fragments arranged by size:
3351 354 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 550 1479 1612 2071 2280 550
Size: 929 133 459 209 2166
Fragments arranged by size:
2166 929 459 209 133
TatI w'GTAC_w
Cuts at: 833 3411 833
Size: 2578 1318
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 152 184 3413 3445 152
Size: 32 3229 32 603
Fragments arranged by size:
3229 603 32 32
Tth111I GACn'n_nGTC
Cuts at: 1693 1693
Size: 3896
XbaI T'CTAG_A
Cuts at: 927 927
Size: 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1391 1391
Size: 3896
XmaI C'CCGG_G
Cuts at: 916 916
Size: 3896
XmnI GAAnn'nnTTC
Cuts at: 433 433
Size: 3896
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsiWI
BsmBI BssHII BstXI BstZ17I BtgI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI