Restriction map for
pF1KB6332
(Circular) (Six-base) MAPSORT of: pF1KB6332.seq Check: 9816 from: 1 to: 3977
pF1KB6332 3977 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 1015 1015
Size: 3977
Acc65I G'GTAC_C
Cuts at: 993 993
Size: 3977
AclI AA'CG_TT
Cuts at: 3817 3817
Size: 3977
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 803 1802 2234 2981 3249 803
Size: 999 432 747 268 1531
Fragments arranged by size:
1531 999 747 432 268
AflIII A'CryG_T
Cuts at: 1329 2433 3410 1329
Size: 1104 977 1896
Fragments arranged by size:
1896 1104 977
AgeI A'CCGG_T
Cuts at: 2365 2365
Size: 3977
AlwNI CAG_nnn'CTG
Cuts at: 802 827 840 1282 2849 802
Size: 25 13 442 1567 1930
Fragments arranged by size:
1930 1567 442 25 13
ApaLI G'TGCA_C
Cuts at: 2747 2747
Size: 3977
ApoI r'AATT_y
Cuts at: 896 981 3330 3586 896
Size: 85 2349 256 1287
Fragments arranged by size:
2349 1287 256 85
AseI AT'TA_AT
Cuts at: 20 20
Size: 3977
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
AvaI C'yCGr_G
Cuts at: 997 997
Size: 3977
BamHI G'GATC_C
Cuts at: 1002 1002
Size: 3977
BanI G'GyrC_C
Cuts at: 993 1407 1655 1690 993
Size: 414 248 35 3280
Fragments arranged by size:
3280 414 248 35
BanII G_rGCy'C
Cuts at: 991 2021 991
Size: 1030 2947
BbeI G_GCGC'C
Cuts at: 1411 1659 1411
Size: 248 3729
BbsI GAAGACnn'nnnn_
Cuts at: 209 791 209
Size: 582 3395
BciVI GTATCCnnnnn_n'
Cuts at: 1875 2636 3977 1875
Size: 761 1341 1875
Fragments arranged by size:
1875 1341 761
BclI T'GATC_A
Cuts at: 1497 1497
Size: 3977
BfrBI ATG'CAT
Cuts at: 136 210 858 136
Size: 74 648 3255
Fragments arranged by size:
3255 648 74
BglI GCCn_nnn'nGGC
Cuts at: 358 723 358
Size: 365 3612
BglII A'GATC_T
Cuts at: 1492 3529 1492
Size: 2037 1940
BlpI GC'TnA_GC
Cuts at: 1089 1089
Size: 3977
Bme1580I G_kGCm'C
Cuts at: 961 1602 1695 2751 961
Size: 641 93 1056 2187
Fragments arranged by size:
2187 1056 641 93
BmrI ACTGGGnnnn_n'
Cuts at: 784 1189 1270 1605 3749 784
Size: 405 81 335 2144 1012
Fragments arranged by size:
2144 1012 405 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 3977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 649 557
Size: 92 3885
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 735 1153 2032 2524 2822 735
Size: 418 879 492 298 1890
Fragments arranged by size:
1890 879 492 418 298
BsaI GGTCTCn'nnnn_
Cuts at: 3681 3681
Size: 3977
BsaAI yAC'GTr
Cuts at: 1330 1330
Size: 3977
BsaBI GATnn'nnATC
Cuts at: 6 1491 3528 6
Size: 1485 2037 455
Fragments arranged by size:
2037 1485 455
BsaHI Gr'CG_yC
Cuts at: 1408 1656 2358 3854 1408
Size: 248 702 1496 1531
Fragments arranged by size:
1531 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1166 1687 2365 2639 2786 3197 1166
Size: 521 678 274 147 411 1946
Fragments arranged by size:
1946 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 580 896 899 947 580
Size: 316 3 48 3610
Fragments arranged by size:
3610 316 48 3
BseYI C'CCAG_C
Cuts at: 833 891 1402 2160 2737 3233 833
Size: 58 511 758 577 496 1577
Fragments arranged by size:
1577 758 577 511 496 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 788 959 250
Size: 538 171 3268
Fragments arranged by size:
3268 538 171
BsiEI CG_ry'CG
Cuts at: 104 1519 1565 2773 104
Size: 1415 46 1208 1308
Fragments arranged by size:
1415 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 991 1769 1959 2751 991
Size: 778 190 792 2217
Fragments arranged by size:
2217 792 778 190
BsmI GAATG_Cn'
Cuts at: 136 136
Size: 3977
Bsp1286I G_dGCh'C
Cuts at: 961 991 1602 1695 1769 1959 2021 2751 961
Size: 30 611 93 74 190 62 730 2187
Fragments arranged by size:
2187 730 611 190 93 74 62 30
BspEI T'CCGG_A
Cuts at: 3197 3197
Size: 3977
BspHI T'CATG_A
Cuts at: 3153 3153
Size: 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1027 1241 1543 1924 1027
Size: 214 302 381 3080
Fragments arranged by size:
3080 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2269 2323 3807 3972 50
Size: 2219 54 1484 165 55
Fragments arranged by size:
2219 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1889 1889
Size: 3977
BsrFI r'CCGG_y
Cuts at: 1975 2156 2365 1975
Size: 181 209 3587
Fragments arranged by size:
3587 209 181
BssSI C'ACGA_G
Cuts at: 2248 2606 2248
Size: 358 3619
BstAPI GCAn_nnn'nTGC
Cuts at: 1354 1354
Size: 3977
BstBI TT'CG_AA
Cuts at: 2338 2338
Size: 3977
BstYI r'GATC_y
Cuts at: 1 1002 1469 1492 1827 2073 3085 3171 3183
Size: 1001 467 23 335 246 1012 86 12
Cuts at: 3183 3529 1
Size: 346 449
Fragments arranged by size:
1012 1001 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 766 766
Size: 3977
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1927 2108 2271 1927
Size: 181 163 3633
Fragments arranged by size:
3633 181 163
BtsI GCAGTG_nn'
Cuts at: 1220 1220
Size: 3977
DraI TTT'AAA
Cuts at: 976 976
Size: 3977
DraIII CAC_nnn'GTG
Cuts at: 343 343
Size: 3977
DrdI GACnn_nn'nnGTC
Cuts at: 463 1683 2541 463
Size: 1220 858 1899
Fragments arranged by size:
1899 1220 858
EaeI y'GGCC_r
Cuts at: 345 1562 1736 2127 2154 3499 345
Size: 1217 174 391 27 1345 823
Fragments arranged by size:
1345 1217 823 391 174 27
EagI C'GGCC_G
Cuts at: 1562 1562
Size: 3977
EarI CTCTTCn'nnn_
Cuts at: 2000 2210 2000
Size: 210 3767
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 586 2185 2495 2641 3787 586
Size: 1599 310 146 1146 776
Fragments arranged by size:
1599 1146 776 310 146
EcoICRI GAG'CTC
Cuts at: 989 989
Size: 3977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 649 803 1802 2234 2981 3249 557
Size: 92 154 999 432 747 268 1285
Fragments arranged by size:
1285 999 747 432 268 154 92
EcoO109I rG'GnC_Cy
Cuts at: 333 1116 333
Size: 783 3194
EcoRI G'AATT_C
Cuts at: 981 981
Size: 3977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1996 1996
Size: 3977
FspI TGC'GCA
Cuts at: 1758 1758
Size: 3977
HaeII r_GCGC'y
Cuts at: 1411 1659 2681 3660 1411
Size: 248 1022 979 1728
Fragments arranged by size:
1728 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 733 765 1819 1849 1851 1881 733
Size: 32 1054 30 2 30 2829
Fragments arranged by size:
2829 1054 32 30 30 2
HincII GTy'rAC
Cuts at: 924 1016 924
Size: 92 3885
HindIII A'AGCT_T
Cuts at: 112 112
Size: 3977
KasI G'GCGC_C
Cuts at: 1407 1655 1407
Size: 248 3729
KpnI G_GTAC'C
Cuts at: 997 997
Size: 3977
MluI A'CGCG_T
Cuts at: 3410 3410
Size: 3977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2648 2832 2648
Size: 184 3793
MscI TGG'CCA
Cuts at: 347 1738 347
Size: 1391 2586
MslI CAynn'nnrTG
Cuts at: 1966 2093 1966
Size: 127 3850
MspA1I CmG'CkG
Cuts at: 370 943 1088 1402 1762 2775 3020 370
Size: 573 145 314 360 1013 245 1327
Fragments arranged by size:
1327 1013 573 360 314 245 145
NaeI GCC'GGC
Cuts at: 2158 2158
Size: 3977
NarI GG'CG_CC
Cuts at: 1408 1656 1408
Size: 248 3729
NgoMIV G'CCGG_C
Cuts at: 2156 2156
Size: 3977
NsiI A_TGCA'T
Cuts at: 138 212 860 138
Size: 74 648 3255
Fragments arranged by size:
3255 648 74
NspI r_CATG'y
Cuts at: 210 862 1030 1530 2437 210
Size: 652 168 500 907 1750
Fragments arranged by size:
1750 907 652 500 168
PciI A'CATG_T
Cuts at: 2433 2433
Size: 3977
PflMI CCAn_nnn'nTGG
Cuts at: 343 840 343
Size: 497 3480
PfoI T'CCnGG_A
Cuts at: 467 554 467
Size: 87 3890
PmeI GTTT'AAAC
Cuts at: 976 976
Size: 3977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3116 3148 3116
Size: 32 3945
PpuMI rG'GwC_Cy
Cuts at: 333 333
Size: 3977
PstI C_TGCA'G
Cuts at: 369 1024 1709 369
Size: 655 685 2637
Fragments arranged by size:
2637 685 655
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3977
PvuII CAG'CTG
Cuts at: 370 943 1402 1762 370
Size: 573 459 360 2585
Fragments arranged by size:
2585 573 459 360
RsrII CG'GwC_CG
Cuts at: 2172 2172
Size: 3977
SacI G_AGCT'C
Cuts at: 991 991
Size: 3977
SalI G'TCGA_C
Cuts at: 1014 1014
Size: 3977
SapI GCTCTTCn'nnn_
Cuts at: 2000 2210 2000
Size: 210 3767
SbfI CC_TGCA'GG
Cuts at: 1024 1024
Size: 3977
ScaI AGT'ACT
Cuts at: 3494 3494
Size: 3977
SfcI C'TryA_G
Cuts at: 33 365 1020 1705 2698 2889 33
Size: 332 655 685 993 191 1121
Fragments arranged by size:
1121 993 685 655 332 191
SfoI GGC'GCC
Cuts at: 1409 1657 1409
Size: 248 3729
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
SmaI CCC'GGG
Cuts at: 999 999
Size: 3977
SmlI C'TyrA_G
Cuts at: 750 1132 2047 2539 2801 750
Size: 382 915 492 262 1926
Fragments arranged by size:
1926 915 492 382 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3977
SphI G_CATG'C
Cuts at: 862 1030 1530 862
Size: 168 500 3309
Fragments arranged by size:
3309 500 168
StyI C'CwwG_G
Cuts at: 1111 1465 1111
Size: 354 3623
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1560 1693 2152 2361 1560
Size: 133 459 209 3176
Fragments arranged by size:
3176 459 209 133
TatI w'GTAC_w
Cuts at: 3492 3492
Size: 3977
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3494 3526 3494
Size: 32 3945
Tth111I GACn'n_nGTC
Cuts at: 798 1774 798
Size: 976 3001
XbaI T'CTAG_A
Cuts at: 1008 1008
Size: 3977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1472 1472
Size: 3977
XmaI C'CCGG_G
Cuts at: 997 997
Size: 3977
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI PacI
PasI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI