Restriction map for
pF1KB6331
(Circular) (Six-base) MAPSORT of: pF1KB6331.seq Check: 1467 from: 1 to: 3896
pF1KB6331 3896 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 934 934
Size: 3896
Acc65I G'GTAC_C
Cuts at: 912 912
Size: 3896
AclI AA'CG_TT
Cuts at: 3736 3736
Size: 3896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 708 720 1721 2153 2900 3168 708
Size: 12 1001 432 747 268 1436
Fragments arranged by size:
1436 1001 747 432 268 12
AflIII A'CryG_T
Cuts at: 1248 2352 3329 1248
Size: 1104 977 1815
Fragments arranged by size:
1815 1104 977
AgeI A'CCGG_T
Cuts at: 2284 2284
Size: 3896
AlwNI CAG_nnn'CTG
Cuts at: 261 1201 2768 261
Size: 940 1567 1389
Fragments arranged by size:
1567 1389 940
ApaLI G'TGCA_C
Cuts at: 2666 2666
Size: 3896
ApoI r'AATT_y
Cuts at: 900 3249 3505 900
Size: 2349 256 1291
Fragments arranged by size:
2349 1291 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
AvaI C'yCGr_G
Cuts at: 557 916 557
Size: 359 3537
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 212 245 256 289 212
Size: 33 11 33 3819
Fragments arranged by size:
3819 33 33 11
BamHI G'GATC_C
Cuts at: 921 921
Size: 3896
BanI G'GyrC_C
Cuts at: 632 912 1326 1574 1609 632
Size: 280 414 248 35 2919
Fragments arranged by size:
2919 414 280 248 35
BanII G_rGCy'C
Cuts at: 910 1940 910
Size: 1030 2866
BbeI G_GCGC'C
Cuts at: 1330 1578 1330
Size: 248 3648
BbvCI CC'TCA_GC
Cuts at: 127 361 127
Size: 234 3662
BciVI GTATCCnnnnn_n'
Cuts at: 293 1794 2555 3896 293
Size: 1501 761 1341 293
Fragments arranged by size:
1501 1341 761 293
BclI T'GATC_A
Cuts at: 1416 1416
Size: 3896
BglII A'GATC_T
Cuts at: 1411 3448 1411
Size: 2037 1859
BlpI GC'TnA_GC
Cuts at: 1008 1008
Size: 3896
Bme1580I G_kGCm'C
Cuts at: 1521 1614 2670 1521
Size: 93 1056 2747
Fragments arranged by size:
2747 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 264 1108 1189 1524 3668 264
Size: 844 81 335 2144 492
Fragments arranged by size:
2144 844 492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 135 826 2263 135
Size: 691 1437 1768
Fragments arranged by size:
1768 1437 691
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 795 460
Size: 335 3561
Bpu10I CC'TnA_GC
Cuts at: 127 254 361 127
Size: 127 107 3662
Fragments arranged by size:
3662 127 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 1072 1951 2443 2741 685
Size: 387 879 492 298 1840
Fragments arranged by size:
1840 879 492 387 298
BsaI GGTCTCn'nnnn_
Cuts at: 3600 3600
Size: 3896
BsaAI yAC'GTr
Cuts at: 1249 1249
Size: 3896
BsaBI GATnn'nnATC
Cuts at: 6 1410 3447 6
Size: 1404 2037 455
Fragments arranged by size:
2037 1404 455
BsaHI Gr'CG_yC
Cuts at: 1327 1575 2277 3773 1327
Size: 248 702 1496 1450
Fragments arranged by size:
1496 1450 702 248
BsaWI w'CCGG_w
Cuts at: 1085 1606 2284 2558 2705 3116 1085
Size: 521 678 274 147 411 1865
Fragments arranged by size:
1865 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 796 826 796
Size: 30 3866
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 115 804 115
Size: 689 3207
BseYI C'CCAG_C
Cuts at: 249 344 636 798 1321 2079 2656 3152 249
Size: 95 292 162 523 758 577 496 993
Fragments arranged by size:
993 758 577 523 496 292 162 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 440 484 357
Size: 83 44 3769
Fragments arranged by size:
3769 83 44
BsiEI CG_ry'CG
Cuts at: 104 1438 1484 2692 104
Size: 1334 46 1208 1308
Fragments arranged by size:
1334 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 317 910 1688 1878 2670 317
Size: 593 778 190 792 1543
Fragments arranged by size:
1543 792 778 593 190
Bsp1286I G_dGCh'C
Cuts at: 317 910 1521 1614 1688 1878 1940 2670 317
Size: 593 611 93 74 190 62 730 1543
Fragments arranged by size:
1543 730 611 593 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3116 3116
Size: 3896
BspHI T'CATG_A
Cuts at: 3072 3072
Size: 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 946 1160 1462 1843 946
Size: 214 302 381 2999
Fragments arranged by size:
2999 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2188 2242 3726 3891 50
Size: 2138 54 1484 165 55
Fragments arranged by size:
2138 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 212 1808 212
Size: 1596 2300
BsrFI r'CCGG_y
Cuts at: 383 1894 2075 2284 383
Size: 1511 181 209 1995
Fragments arranged by size:
1995 1511 209 181
BssSI C'ACGA_G
Cuts at: 191 2167 2525 191
Size: 1976 358 1562
Fragments arranged by size:
1976 1562 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1273 1273
Size: 3896
BstBI TT'CG_AA
Cuts at: 2257 2257
Size: 3896
BstYI r'GATC_y
Cuts at: 1 921 1388 1411 1746 1992 3004 3090 3102
Size: 920 467 23 335 246 1012 86 12
Cuts at: 3102 3448 1
Size: 346 449
Fragments arranged by size:
1012 920 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 856 856
Size: 3896
BtgI C'CryG_G
Cuts at: 107 107
Size: 3896
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1846 2027 2190 1846
Size: 181 163 3552
Fragments arranged by size:
3552 181 163
BtsI GCAGTG_nn'
Cuts at: 1139 1139
Size: 3896
DraI TTT'AAA
Cuts at: 895 895
Size: 3896
DrdI GACnn_nn'nnGTC
Cuts at: 1602 2460 1602
Size: 858 3038
EaeI y'GGCC_r
Cuts at: 1481 1655 2046 2073 3418 1481
Size: 174 391 27 1345 1959
Fragments arranged by size:
1959 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1481 1481
Size: 3896
EarI CTCTTCn'nnn_
Cuts at: 156 537 1919 2129 156
Size: 381 1382 210 1923
Fragments arranged by size:
1923 1382 381 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 147 381 2104 2414 2560 3706 147
Size: 234 1723 310 146 1146 337
Fragments arranged by size:
1723 1146 337 310 234 146
EcoICRI GAG'CTC
Cuts at: 908 908
Size: 3896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 708 720 795 1721 2153 2900 3168 460
Size: 248 12 75 926 432 747 268 1188
Fragments arranged by size:
1188 926 747 432 268 248 75 12
EcoNI CCTnn'n_nnAGG
Cuts at: 499 499
Size: 3896
EcoO109I rG'GnC_Cy
Cuts at: 258 1035 258
Size: 777 3119
EcoRI G'AATT_C
Cuts at: 900 900
Size: 3896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 738 1915 738
Size: 1177 2719
FspI TGC'GCA
Cuts at: 1677 1677
Size: 3896
HaeII r_GCGC'y
Cuts at: 1330 1578 2600 3579 1330
Size: 248 1022 979 1647
Fragments arranged by size:
1647 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 131 163 796 828 1738 1768 1770 1800 131
Size: 32 633 32 910 30 2 30 2227
Fragments arranged by size:
2227 910 633 32 32 30 30 2
HincII GTy'rAC
Cuts at: 935 935
Size: 3896
KasI G'GCGC_C
Cuts at: 1326 1574 1326
Size: 248 3648
KpnI G_GTAC'C
Cuts at: 916 916
Size: 3896
MluI A'CGCG_T
Cuts at: 3329 3329
Size: 3896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2751 2567
Size: 184 3712
MscI TGG'CCA
Cuts at: 1657 1657
Size: 3896
MslI CAynn'nnrTG
Cuts at: 616 1885 2012 616
Size: 1269 127 2500
Fragments arranged by size:
2500 1269 127
MspA1I CmG'CkG
Cuts at: 438 740 824 1007 1321 1681 2694 2939 438
Size: 302 84 183 314 360 1013 245 1395
Fragments arranged by size:
1395 1013 360 314 302 245 183 84
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 3896
NarI GG'CG_CC
Cuts at: 1327 1575 1327
Size: 248 3648
NcoI C'CATG_G
Cuts at: 107 107
Size: 3896
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 3896
NspI r_CATG'y
Cuts at: 681 949 1449 2356 681
Size: 268 500 907 2221
Fragments arranged by size:
2221 907 500 268
PciI A'CATG_T
Cuts at: 2352 2352
Size: 3896
PmeI GTTT'AAAC
Cuts at: 895 895
Size: 3896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 3864
PstI C_TGCA'G
Cuts at: 331 943 1628 331
Size: 612 685 2599
Fragments arranged by size:
2599 685 612
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3896
PvuII CAG'CTG
Cuts at: 438 740 824 1321 1681 438
Size: 302 84 497 360 2653
Fragments arranged by size:
2653 497 360 302 84
RsrII CG'GwC_CG
Cuts at: 2091 2091
Size: 3896
SacI G_AGCT'C
Cuts at: 910 910
Size: 3896
SalI G'TCGA_C
Cuts at: 933 933
Size: 3896
SapI GCTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
SbfI CC_TGCA'GG
Cuts at: 943 943
Size: 3896
ScaI AGT'ACT
Cuts at: 3413 3413
Size: 3896
SfcI C'TryA_G
Cuts at: 33 327 402 939 1624 2617 2808 33
Size: 294 75 537 685 993 191 1121
Fragments arranged by size:
1121 993 685 537 294 191 75
SfoI GGC'GCC
Cuts at: 1328 1576 1328
Size: 248 3648
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
SmaI CCC'GGG
Cuts at: 918 918
Size: 3896
SmlI C'TyrA_G
Cuts at: 557 700 1051 1966 2458 2720 557
Size: 143 351 915 492 262 1733
Fragments arranged by size:
1733 915 492 351 262 143
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3896
SphI G_CATG'C
Cuts at: 949 1449 949
Size: 500 3396
StuI AGG'CCT
Cuts at: 445 832 445
Size: 387 3509
StyI C'CwwG_G
Cuts at: 107 1030 1384 107
Size: 923 354 2619
Fragments arranged by size:
2619 923 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 358 650 1479 1612 2071 2280 358
Size: 292 829 133 459 209 1974
Fragments arranged by size:
1974 829 459 292 209 133
TatI w'GTAC_w
Cuts at: 749 3411 749
Size: 2662 1234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 3864
Tth111I GACn'n_nGTC
Cuts at: 1693 1693
Size: 3896
XbaI T'CTAG_A
Cuts at: 927 927
Size: 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 585 1391 585
Size: 806 3090
XhoI C'TCGA_G
Cuts at: 557 557
Size: 3896
XmaI C'CCGG_G
Cuts at: 916 916
Size: 3896
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbsI BcgI BfrBI BglI BmgBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI