Restriction map for
pF1KB6330
(Circular) (Six-base) MAPSORT of: pF1KB6330.seq Check: 7767 from: 1 to: 3977
pF1KB6330 3977 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 375 375
Size: 3977
AatII G_ACGT'C
Cuts at: 863 863
Size: 3977
AccI GT'mk_AC
Cuts at: 1015 1015
Size: 3977
Acc65I G'GTAC_C
Cuts at: 993 993
Size: 3977
AclI AA'CG_TT
Cuts at: 3817 3817
Size: 3977
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 1802 2234 2981 3249 127
Size: 1675 432 747 268 855
Fragments arranged by size:
1675 855 747 432 268
AflIII A'CryG_T
Cuts at: 1329 2433 3410 1329
Size: 1104 977 1896
Fragments arranged by size:
1896 1104 977
AgeI A'CCGG_T
Cuts at: 2365 2365
Size: 3977
AlwNI CAG_nnn'CTG
Cuts at: 192 390 687 1282 2849 192
Size: 198 297 595 1567 1320
Fragments arranged by size:
1567 1320 595 297 198
ApaLI G'TGCA_C
Cuts at: 2747 2747
Size: 3977
ApoI r'AATT_y
Cuts at: 981 3330 3586 981
Size: 2349 256 1372
Fragments arranged by size:
2349 1372 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3977
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
AvaI C'yCGr_G
Cuts at: 205 997 205
Size: 792 3185
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 908 941 908
Size: 33 3944
BamHI G'GATC_C
Cuts at: 320 1002 320
Size: 682 3295
BanI G'GyrC_C
Cuts at: 993 1407 1655 1690 993
Size: 414 248 35 3280
Fragments arranged by size:
3280 414 248 35
BanII G_rGCy'C
Cuts at: 206 346 991 2021 206
Size: 140 645 1030 2162
Fragments arranged by size:
2162 1030 645 140
BbeI G_GCGC'C
Cuts at: 1411 1659 1411
Size: 248 3729
BbsI GAAGACnn'nnnn_
Cuts at: 945 945
Size: 3977
BciVI GTATCCnnnnn_n'
Cuts at: 1875 2636 3977 1875
Size: 761 1341 1875
Fragments arranged by size:
1875 1341 761
BclI T'GATC_A
Cuts at: 353 1497 353
Size: 1144 2833
BglI GCCn_nnn'nGGC
Cuts at: 698 698
Size: 3977
BglII A'GATC_T
Cuts at: 581 1492 3529 581
Size: 911 2037 1029
Fragments arranged by size:
2037 1029 911
BlpI GC'TnA_GC
Cuts at: 380 680 1089 380
Size: 300 409 3268
Fragments arranged by size:
3268 409 300
Bme1580I G_kGCm'C
Cuts at: 245 1602 1695 2751 245
Size: 1357 93 1056 1471
Fragments arranged by size:
1471 1357 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 727 1189 1270 1605 3749 727
Size: 462 81 335 2144 955
Fragments arranged by size:
2144 955 462 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 764 2344 764
Size: 1580 2397
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 886
Size: 3977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 1153 2032 2524 2822 148
Size: 1005 879 492 298 1303
Fragments arranged by size:
1303 1005 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3681 3681
Size: 3977
BsaAI yAC'GTr
Cuts at: 1330 1330
Size: 3977
BsaBI GATnn'nnATC
Cuts at: 6 1491 3528 6
Size: 1485 2037 455
Fragments arranged by size:
2037 1485 455
BsaHI Gr'CG_yC
Cuts at: 860 1408 1656 2358 3854 860
Size: 548 248 702 1496 983
Fragments arranged by size:
1496 983 702 548 248
BsaWI w'CCGG_w
Cuts at: 1166 1687 2365 2639 2786 3197 1166
Size: 521 678 274 147 411 1946
Fragments arranged by size:
1946 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 436 571 574 163
Size: 273 135 3 3566
Fragments arranged by size:
3566 273 135 3
BseYI C'CCAG_C
Cuts at: 275 654 1402 2160 2737 3233 275
Size: 379 748 758 577 496 1019
Fragments arranged by size:
1019 758 748 577 496 379
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 460
Size: 3977
BsiEI CG_ry'CG
Cuts at: 104 1519 1565 2773 104
Size: 1415 46 1208 1308
Fragments arranged by size:
1415 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 206 407 821 991 1769 1959 2751 206
Size: 201 414 170 778 190 792 1432
Fragments arranged by size:
1432 792 778 414 201 190 170
Bsp1286I G_dGCh'C
Cuts at: 206 245 346 407 821 991 1602 1695 1769
Size: 39 101 61 414 170 611 93 74
Cuts at: 1769 1959 2021 2751 206
Size: 190 62 730 1432
Fragments arranged by size:
1432 730 611 414 190 170 101 93
74 62 61 39
BspEI T'CCGG_A
Cuts at: 3197 3197
Size: 3977
BspHI T'CATG_A
Cuts at: 3153 3153
Size: 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 191 375 432 1027 1241 1543 1924 191
Size: 184 57 595 214 302 381 2244
Fragments arranged by size:
2244 595 381 302 214 184 57
BsrBI CCG'CTC
Cuts at: 50 806 2269 2323 3807 3972 50
Size: 756 1463 54 1484 165 55
Fragments arranged by size:
1484 1463 756 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1889 1889
Size: 3977
BsrFI r'CCGG_y
Cuts at: 428 1975 2156 2365 428
Size: 1547 181 209 2040
Fragments arranged by size:
2040 1547 209 181
BssSI C'ACGA_G
Cuts at: 2248 2606 2248
Size: 358 3619
BstAPI GCAn_nnn'nTGC
Cuts at: 390 1354 390
Size: 964 3013
BstBI TT'CG_AA
Cuts at: 2338 2338
Size: 3977
BstEII G'GTnAC_C
Cuts at: 468 468
Size: 3977
BstXI CCAn_nnnn'nTGG
Cuts at: 505 505
Size: 3977
BstYI r'GATC_y
Cuts at: 1 248 320 581 767 869 1002 1469 1492
Size: 247 72 261 186 102 133 467 23
Cuts at: 1492 1827 2073 3085 3171 3183 3529 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 261 247 246
186 133 102 86 72 23 12
Bsu36I CC'TnA_GG
Cuts at: 544 544
Size: 3977
BtgI C'CryG_G
Cuts at: 464 891 464
Size: 427 3550
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 629 1927 2108 2271 629
Size: 1298 181 163 2335
Fragments arranged by size:
2335 1298 181 163
BtsI GCAGTG_nn'
Cuts at: 324 1220 324
Size: 896 3081
DraI TTT'AAA
Cuts at: 976 976
Size: 3977
DraIII CAC_nnn'GTG
Cuts at: 721 721
Size: 3977
DrdI GACnn_nn'nnGTC
Cuts at: 1683 2541 1683
Size: 858 3119
EaeI y'GGCC_r
Cuts at: 507 699 1562 1736 2127 2154 3499 507
Size: 192 863 174 391 27 1345 985
Fragments arranged by size:
1345 985 863 391 192 174 27
EagI C'GGCC_G
Cuts at: 1562 1562
Size: 3977
EarI CTCTTCn'nnn_
Cuts at: 2000 2210 2000
Size: 210 3767
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2185 2495 2641 3787 2185
Size: 310 146 1146 2375
Fragments arranged by size:
2375 1146 310 146
EcoICRI GAG'CTC
Cuts at: 204 989 204
Size: 785 3192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 886 1802 2234 2981 3249 127
Size: 759 916 432 747 268 855
Fragments arranged by size:
916 855 759 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 145 145
Size: 3977
EcoO109I rG'GnC_Cy
Cuts at: 180 228 282 912 942 1116 180
Size: 48 54 630 30 174 3041
Fragments arranged by size:
3041 630 174 54 48 30
EcoRI G'AATT_C
Cuts at: 981 981
Size: 3977
EcoRV GAT'ATC
Cuts at: 834 834
Size: 3977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1996 1996
Size: 3977
FspI TGC'GCA
Cuts at: 1758 1758
Size: 3977
HaeII r_GCGC'y
Cuts at: 1411 1659 2681 3660 1411
Size: 248 1022 979 1728
Fragments arranged by size:
1728 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 772 804 1819 1849 1851 1881 772
Size: 32 1015 30 2 30 2868
Fragments arranged by size:
2868 1015 32 30 30 2
HincII GTy'rAC
Cuts at: 1016 1016
Size: 3977
KasI G'GCGC_C
Cuts at: 1407 1655 1407
Size: 248 3729
KpnI G_GTAC'C
Cuts at: 997 997
Size: 3977
MluI A'CGCG_T
Cuts at: 3410 3410
Size: 3977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2648 2832 2648
Size: 184 3793
MscI TGG'CCA
Cuts at: 1738 1738
Size: 3977
MslI CAynn'nnrTG
Cuts at: 481 1966 2093 481
Size: 1485 127 2365
Fragments arranged by size:
2365 1485 127
MspA1I CmG'CkG
Cuts at: 192 893 1088 1402 1762 2775 3020 192
Size: 701 195 314 360 1013 245 1149
Fragments arranged by size:
1149 1013 701 360 314 245 195
NaeI GCC'GGC
Cuts at: 430 2158 430
Size: 1728 2249
NarI GG'CG_CC
Cuts at: 1408 1656 1408
Size: 248 3729
NcoI C'CATG_G
Cuts at: 464 464
Size: 3977
NgoMIV G'CCGG_C
Cuts at: 428 2156 428
Size: 1728 2249
NspI r_CATG'y
Cuts at: 1030 1530 2437 1030
Size: 500 907 2570
Fragments arranged by size:
2570 907 500
PciI A'CATG_T
Cuts at: 2433 2433
Size: 3977
PflMI CCAn_nnn'nTGG
Cuts at: 453 486 721 453
Size: 33 235 3709
Fragments arranged by size:
3709 235 33
PfoI T'CCnGG_A
Cuts at: 863 863
Size: 3977
PmeI GTTT'AAAC
Cuts at: 976 976
Size: 3977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3116 3148 3116
Size: 32 3945
PpuMI rG'GwC_Cy
Cuts at: 180 228 282 180
Size: 48 54 3875
Fragments arranged by size:
3875 54 48
PstI C_TGCA'G
Cuts at: 188 1024 1709 188
Size: 836 685 2456
Fragments arranged by size:
2456 836 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3977
PvuII CAG'CTG
Cuts at: 192 1402 1762 192
Size: 1210 360 2407
Fragments arranged by size:
2407 1210 360
RsrII CG'GwC_CG
Cuts at: 2172 2172
Size: 3977
SacI G_AGCT'C
Cuts at: 206 991 206
Size: 785 3192
SacII CC_GC'GG
Cuts at: 894 894
Size: 3977
SalI G'TCGA_C
Cuts at: 1014 1014
Size: 3977
SapI GCTCTTCn'nnn_
Cuts at: 2000 2210 2000
Size: 210 3767
SbfI CC_TGCA'GG
Cuts at: 1024 1024
Size: 3977
ScaI AGT'ACT
Cuts at: 3494 3494
Size: 3977
SfcI C'TryA_G
Cuts at: 33 184 1020 1705 2698 2889 33
Size: 151 836 685 993 191 1121
Fragments arranged by size:
1121 993 836 685 191 151
SfoI GGC'GCC
Cuts at: 1409 1657 1409
Size: 248 3729
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
SmaI CCC'GGG
Cuts at: 999 999
Size: 3977
SmlI C'TyrA_G
Cuts at: 163 1132 2047 2539 2801 163
Size: 969 915 492 262 1339
Fragments arranged by size:
1339 969 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3977
SphI G_CATG'C
Cuts at: 1030 1530 1030
Size: 500 3477
StyI C'CwwG_G
Cuts at: 452 464 930 1111 1465 452
Size: 12 466 181 354 2964
Fragments arranged by size:
2964 466 354 181 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 289 709 1560 1693 2152 2361 289
Size: 420 851 133 459 209 1905
Fragments arranged by size:
1905 851 459 420 209 133
TatI w'GTAC_w
Cuts at: 3492 3492
Size: 3977
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 516 548 594 626 3494 3526 516
Size: 32 46 32 2868 32 967
Fragments arranged by size:
2868 967 46 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 226 1774 226
Size: 1548 2429
XbaI T'CTAG_A
Cuts at: 1008 1008
Size: 3977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 252 483 1472 252
Size: 231 989 2757
Fragments arranged by size:
2757 989 231
XmaI C'CCGG_G
Cuts at: 997 997
Size: 3977
ZraI GAC'GTC
Cuts at: 861 861
Size: 3977
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I ClaI CspCI
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI