Restriction map for
pF1KB6326
(Circular) (Six-base) MAPSORT of: pF1KB6326.seq Check: 5242 from: 1 to: 3971
pF1KB6326 3971 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 1009 1009
Size: 3971
Acc65I G'GTAC_C
Cuts at: 987 987
Size: 3971
AclI AA'CG_TT
Cuts at: 3811 3811
Size: 3971
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 1796 2228 2975 3243 359
Size: 1437 432 747 268 1087
Fragments arranged by size:
1437 1087 747 432 268
AflIII A'CryG_T
Cuts at: 1323 2427 3404 1323
Size: 1104 977 1890
Fragments arranged by size:
1890 1104 977
AgeI A'CCGG_T
Cuts at: 2359 2359
Size: 3971
AleI CACnn'nnGTG
Cuts at: 541 541
Size: 3971
AlwNI CAG_nnn'CTG
Cuts at: 1276 2843 1276
Size: 1567 2404
ApaLI G'TGCA_C
Cuts at: 2741 2741
Size: 3971
ApoI r'AATT_y
Cuts at: 513 975 3324 3580 513
Size: 462 2349 256 904
Fragments arranged by size:
2349 904 462 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3971
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3971
AvaI C'yCGr_G
Cuts at: 991 991
Size: 3971
BamHI G'GATC_C
Cuts at: 996 996
Size: 3971
BanI G'GyrC_C
Cuts at: 987 1401 1649 1684 987
Size: 414 248 35 3274
Fragments arranged by size:
3274 414 248 35
BanII G_rGCy'C
Cuts at: 596 985 2015 596
Size: 389 1030 2552
Fragments arranged by size:
2552 1030 389
BbeI G_GCGC'C
Cuts at: 1405 1653 1405
Size: 248 3723
BbsI GAAGACnn'nnnn_
Cuts at: 666 666
Size: 3971
BciVI GTATCCnnnnn_n'
Cuts at: 1869 2630 3971 1869
Size: 761 1341 1869
Fragments arranged by size:
1869 1341 761
BclI T'GATC_A
Cuts at: 1491 1491
Size: 3971
BglI GCCn_nnn'nGGC
Cuts at: 185 185
Size: 3971
BglII A'GATC_T
Cuts at: 207 1486 3523 207
Size: 1279 2037 655
Fragments arranged by size:
2037 1279 655
BlpI GC'TnA_GC
Cuts at: 1083 1083
Size: 3971
Bme1580I G_kGCm'C
Cuts at: 939 1596 1689 2745 939
Size: 657 93 1056 2165
Fragments arranged by size:
2165 1056 657 93
BmrI ACTGGGnnnn_n'
Cuts at: 1183 1264 1599 3743 1183
Size: 81 335 2144 1411
Fragments arranged by size:
2144 1411 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2338 2338
Size: 3971
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1147 2026 2518 2816 1147
Size: 879 492 298 2302
Fragments arranged by size:
2302 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 807 3675 807
Size: 2868 1103
BsaAI yAC'GTr
Cuts at: 1324 1324
Size: 3971
BsaBI GATnn'nnATC
Cuts at: 6 1485 3522 6
Size: 1479 2037 455
Fragments arranged by size:
2037 1479 455
BsaHI Gr'CG_yC
Cuts at: 1402 1650 2352 3848 1402
Size: 248 702 1496 1525
Fragments arranged by size:
1525 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1160 1681 2359 2633 2780 3191 1160
Size: 521 678 274 147 411 1940
Fragments arranged by size:
1940 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 106 106
Size: 3971
BseYI C'CCAG_C
Cuts at: 1396 2154 2731 3227 1396
Size: 758 577 496 2140
Fragments arranged by size:
2140 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 374
Size: 3971
BsiEI CG_ry'CG
Cuts at: 104 1513 1559 2767 104
Size: 1409 46 1208 1308
Fragments arranged by size:
1409 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 985 1763 1953 2745 985
Size: 778 190 792 2211
Fragments arranged by size:
2211 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 596 939 985 1596 1689 1763 1953 2015 2745
Size: 343 46 611 93 74 190 62 730
Cuts at: 2745 596
Size: 1822
Fragments arranged by size:
1822 730 611 343 190 93 74 62
46
BspEI T'CCGG_A
Cuts at: 3191 3191
Size: 3971
BspHI T'CATG_A
Cuts at: 3147 3147
Size: 3971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1021 1235 1537 1918 1021
Size: 214 302 381 3074
Fragments arranged by size:
3074 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2263 2317 3801 3966 50
Size: 2213 54 1484 165 55
Fragments arranged by size:
2213 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 660 1883 660
Size: 1223 2748
BsrFI r'CCGG_y
Cuts at: 1969 2150 2359 1969
Size: 181 209 3581
Fragments arranged by size:
3581 209 181
BssSI C'ACGA_G
Cuts at: 145 2242 2600 145
Size: 2097 358 1516
Fragments arranged by size:
2097 1516 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1348 1348
Size: 3971
BstBI TT'CG_AA
Cuts at: 2332 2332
Size: 3971
BstXI CCAn_nnnn'nTGG
Cuts at: 297 297
Size: 3971
BstYI r'GATC_y
Cuts at: 1 207 955 996 1463 1486 1821 2067 3079
Size: 206 748 41 467 23 335 246 1012
Cuts at: 3079 3165 3177 3523 1
Size: 86 12 346 449
Fragments arranged by size:
1012 748 467 449 346 335 246 206
86 41 23 12
Bsu36I CC'TnA_GG
Cuts at: 488 878 488
Size: 390 3581
BtgI C'CryG_G
Cuts at: 596 596
Size: 3971
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1921 2102 2265 1921
Size: 181 163 3627
Fragments arranged by size:
3627 181 163
BtsI GCAGTG_nn'
Cuts at: 203 1214 203
Size: 1011 2960
DraI TTT'AAA
Cuts at: 970 970
Size: 3971
DraIII CAC_nnn'GTG
Cuts at: 352 352
Size: 3971
DrdI GACnn_nn'nnGTC
Cuts at: 1677 2535 1677
Size: 858 3113
EaeI y'GGCC_r
Cuts at: 1556 1730 2121 2148 3493 1556
Size: 174 391 27 1345 2034
Fragments arranged by size:
2034 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1556 1556
Size: 3971
EarI CTCTTCn'nnn_
Cuts at: 530 937 1994 2204 530
Size: 407 1057 210 2297
Fragments arranged by size:
2297 1057 407 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 617 2179 2489 2635 3781 617
Size: 1562 310 146 1146 807
Fragments arranged by size:
1562 1146 807 310 146
EcoICRI GAG'CTC
Cuts at: 983 983
Size: 3971
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 1796 2228 2975 3243 359
Size: 1437 432 747 268 1087
Fragments arranged by size:
1437 1087 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1110 1110
Size: 3971
EcoRI G'AATT_C
Cuts at: 975 975
Size: 3971
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1990 1990
Size: 3971
FspI TGC'GCA
Cuts at: 1752 1752
Size: 3971
HaeII r_GCGC'y
Cuts at: 1405 1653 2675 3654 1405
Size: 248 1022 979 1722
Fragments arranged by size:
1722 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 713 745 1813 1843 1845 1875 713
Size: 32 1068 30 2 30 2809
Fragments arranged by size:
2809 1068 32 30 30 2
HincII GTy'rAC
Cuts at: 861 1010 861
Size: 149 3822
HindIII A'AGCT_T
Cuts at: 341 905 341
Size: 564 3407
KasI G'GCGC_C
Cuts at: 1401 1649 1401
Size: 248 3723
KpnI G_GTAC'C
Cuts at: 991 991
Size: 3971
MluI A'CGCG_T
Cuts at: 3404 3404
Size: 3971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 850 2642 2826 850
Size: 1792 184 1995
Fragments arranged by size:
1995 1792 184
MscI TGG'CCA
Cuts at: 1732 1732
Size: 3971
MslI CAynn'nnrTG
Cuts at: 541 1960 2087 541
Size: 1419 127 2425
Fragments arranged by size:
2425 1419 127
MspA1I CmG'CkG
Cuts at: 1082 1396 1756 2769 3014 1082
Size: 314 360 1013 245 2039
Fragments arranged by size:
2039 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2152 2152
Size: 3971
NarI GG'CG_CC
Cuts at: 1402 1650 1402
Size: 248 3723
NcoI C'CATG_G
Cuts at: 596 596
Size: 3971
NgoMIV G'CCGG_C
Cuts at: 2150 2150
Size: 3971
NspI r_CATG'y
Cuts at: 1024 1524 2431 1024
Size: 500 907 2564
Fragments arranged by size:
2564 907 500
PciI A'CATG_T
Cuts at: 2427 2427
Size: 3971
PmeI GTTT'AAAC
Cuts at: 970 970
Size: 3971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3110 3142 3110
Size: 32 3939
PstI C_TGCA'G
Cuts at: 591 1018 1703 591
Size: 427 685 2859
Fragments arranged by size:
2859 685 427
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3971
PvuII CAG'CTG
Cuts at: 1396 1756 1396
Size: 360 3611
RsrII CG'GwC_CG
Cuts at: 2166 2166
Size: 3971
SacI G_AGCT'C
Cuts at: 985 985
Size: 3971
SalI G'TCGA_C
Cuts at: 1008 1008
Size: 3971
SapI GCTCTTCn'nnn_
Cuts at: 530 1994 2204 530
Size: 1464 210 2297
Fragments arranged by size:
2297 1464 210
SbfI CC_TGCA'GG
Cuts at: 1018 1018
Size: 3971
ScaI AGT'ACT
Cuts at: 574 3488 574
Size: 2914 1057
SfcI C'TryA_G
Cuts at: 33 539 587 1014 1699 2692 2883 33
Size: 506 48 427 685 993 191 1121
Fragments arranged by size:
1121 993 685 506 427 191 48
SfoI GGC'GCC
Cuts at: 1403 1651 1403
Size: 248 3723
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3971
SmaI CCC'GGG
Cuts at: 993 993
Size: 3971
SmlI C'TyrA_G
Cuts at: 1126 2041 2533 2795 1126
Size: 915 492 262 2302
Fragments arranged by size:
2302 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3971
SphI G_CATG'C
Cuts at: 1024 1524 1024
Size: 500 3471
StyI C'CwwG_G
Cuts at: 596 1105 1459 596
Size: 509 354 3108
Fragments arranged by size:
3108 509 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1554 1687 2146 2355 1554
Size: 133 459 209 3170
Fragments arranged by size:
3170 459 209 133
TatI w'GTAC_w
Cuts at: 572 3486 572
Size: 2914 1057
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 510 542 3488 3520 510
Size: 32 2946 32 961
Fragments arranged by size:
2946 961 32 32
Tth111I GACn'n_nGTC
Cuts at: 1768 1768
Size: 3971
XbaI T'CTAG_A
Cuts at: 799 1002 799
Size: 203 3768
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1466 1466
Size: 3971
XmaI C'CCGG_G
Cuts at: 991 991
Size: 3971
XmnI GAAnn'nnTTC
Cuts at: 389 389
Size: 3971
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI