Restriction map for
pF1KB6324
(Circular) (Six-base) MAPSORT of: pF1KB6324.seq Check: 6245 from: 1 to: 3959
pF1KB6324 3959 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 997 997
Size: 3959
Acc65I G'GTAC_C
Cuts at: 975 975
Size: 3959
AclI AA'CG_TT
Cuts at: 176 3799 176
Size: 3623 336
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1784 2216 2963 3231 1784
Size: 432 747 268 2512
Fragments arranged by size:
2512 747 432 268
AflIII A'CryG_T
Cuts at: 229 1311 2415 3392 229
Size: 1082 1104 977 796
Fragments arranged by size:
1104 1082 977 796
AgeI A'CCGG_T
Cuts at: 2347 2347
Size: 3959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 308 308
Size: 3959
AlwNI CAG_nnn'CTG
Cuts at: 1264 2831 1264
Size: 1567 2392
ApaLI G'TGCA_C
Cuts at: 2729 2729
Size: 3959
ApoI r'AATT_y
Cuts at: 963 3312 3568 963
Size: 2349 256 1354
Fragments arranged by size:
2349 1354 256
AseI AT'TA_AT
Cuts at: 20 359 566 20
Size: 339 207 3413
Fragments arranged by size:
3413 339 207
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3959
AvaI C'yCGr_G
Cuts at: 979 979
Size: 3959
BamHI G'GATC_C
Cuts at: 984 984
Size: 3959
BanI G'GyrC_C
Cuts at: 975 1389 1637 1672 975
Size: 414 248 35 3262
Fragments arranged by size:
3262 414 248 35
BanII G_rGCy'C
Cuts at: 596 973 2003 596
Size: 377 1030 2552
Fragments arranged by size:
2552 1030 377
BbeI G_GCGC'C
Cuts at: 1393 1641 1393
Size: 248 3711
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3925
BciVI GTATCCnnnnn_n'
Cuts at: 1857 2618 3959 1857
Size: 761 1341 1857
Fragments arranged by size:
1857 1341 761
BclI T'GATC_A
Cuts at: 144 1479 144
Size: 1335 2624
BglII A'GATC_T
Cuts at: 1474 3511 1474
Size: 2037 1922
BlpI GC'TnA_GC
Cuts at: 1071 1071
Size: 3959
Bme1580I G_kGCm'C
Cuts at: 1584 1677 2733 1584
Size: 93 1056 2810
Fragments arranged by size:
2810 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1171 1252 1587 3731 1171
Size: 81 335 2144 1399
Fragments arranged by size:
2144 1399 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2326 2326
Size: 3959
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 2014 2506 2804 1135
Size: 879 492 298 2290
Fragments arranged by size:
2290 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 523 3663 523
Size: 3140 819
BsaAI yAC'GTr
Cuts at: 230 1312 230
Size: 1082 2877
BsaBI GATnn'nnATC
Cuts at: 6 1473 3510 6
Size: 1467 2037 455
Fragments arranged by size:
2037 1467 455
BsaHI Gr'CG_yC
Cuts at: 1390 1638 2340 3836 1390
Size: 248 702 1496 1513
Fragments arranged by size:
1513 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1148 1669 2347 2621 2768 3179 1148
Size: 521 678 274 147 411 1928
Fragments arranged by size:
1928 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 476 506 636 666 476
Size: 30 130 30 3769
Fragments arranged by size:
3769 130 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 500 541 500
Size: 41 3918
BseYI C'CCAG_C
Cuts at: 1384 2142 2719 3215 1384
Size: 758 577 496 2128
Fragments arranged by size:
2128 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 410
Size: 3959
BsiEI CG_ry'CG
Cuts at: 104 1501 1547 2755 104
Size: 1397 46 1208 1308
Fragments arranged by size:
1397 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 973 1751 1941 2733 973
Size: 778 190 792 2199
Fragments arranged by size:
2199 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 596 973 1584 1677 1751 1941 2003 2733 596
Size: 377 611 93 74 190 62 730 1822
Fragments arranged by size:
1822 730 611 377 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3179 3179
Size: 3959
BspHI T'CATG_A
Cuts at: 3135 3135
Size: 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1009 1223 1525 1906 1009
Size: 214 302 381 3062
Fragments arranged by size:
3062 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2251 2305 3789 3954 50
Size: 2201 54 1484 165 55
Fragments arranged by size:
2201 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1871 1871
Size: 3959
BsrFI r'CCGG_y
Cuts at: 1957 2138 2347 1957
Size: 181 209 3569
Fragments arranged by size:
3569 209 181
BssSI C'ACGA_G
Cuts at: 2230 2588 2230
Size: 358 3601
BstAPI GCAn_nnn'nTGC
Cuts at: 1336 1336
Size: 3959
BstBI TT'CG_AA
Cuts at: 354 432 2320 354
Size: 78 1888 1993
Fragments arranged by size:
1993 1888 78
BstEII G'GTnAC_C
Cuts at: 454 454
Size: 3959
BstXI CCAn_nnnn'nTGG
Cuts at: 451 451
Size: 3959
BstYI r'GATC_y
Cuts at: 1 128 984 1451 1474 1809 2055 3067 3153
Size: 127 856 467 23 335 246 1012 86
Cuts at: 3153 3165 3511 1
Size: 12 346 449
Fragments arranged by size:
1012 856 467 449 346 335 246 127
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 524 524
Size: 3959
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1909 2090 2253 1909
Size: 181 163 3615
Fragments arranged by size:
3615 181 163
BtsI GCAGTG_nn'
Cuts at: 1202 1202
Size: 3959
DraI TTT'AAA
Cuts at: 327 958 327
Size: 631 3328
DrdI GACnn_nn'nnGTC
Cuts at: 1665 2523 1665
Size: 858 3101
EaeI y'GGCC_r
Cuts at: 161 1544 1718 2109 2136 3481 161
Size: 1383 174 391 27 1345 639
Fragments arranged by size:
1383 1345 639 391 174 27
EagI C'GGCC_G
Cuts at: 1544 1544
Size: 3959
EarI CTCTTCn'nnn_
Cuts at: 332 1982 2192 332
Size: 1650 210 2099
Fragments arranged by size:
2099 1650 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2167 2477 2623 3769 2167
Size: 310 146 1146 2357
Fragments arranged by size:
2357 1146 310 146
EcoICRI GAG'CTC
Cuts at: 971 971
Size: 3959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1784 2216 2963 3231 1784
Size: 432 747 268 2512
Fragments arranged by size:
2512 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 410 410
Size: 3959
EcoO109I rG'GnC_Cy
Cuts at: 1098 1098
Size: 3959
EcoRI G'AATT_C
Cuts at: 963 963
Size: 3959
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 801 1978 801
Size: 1177 2782
FspI TGC'GCA
Cuts at: 1740 1740
Size: 3959
HaeII r_GCGC'y
Cuts at: 1393 1641 2663 3642 1393
Size: 248 1022 979 1710
Fragments arranged by size:
1710 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 370 402 1801 1831 1833 1863 370
Size: 32 1399 30 2 30 2466
Fragments arranged by size:
2466 1399 32 30 30 2
HincII GTy'rAC
Cuts at: 768 998 768
Size: 230 3729
KasI G'GCGC_C
Cuts at: 1389 1637 1389
Size: 248 3711
KpnI G_GTAC'C
Cuts at: 979 979
Size: 3959
MluI A'CGCG_T
Cuts at: 3392 3392
Size: 3959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2630 2814 2630
Size: 184 3775
MscI TGG'CCA
Cuts at: 163 1720 163
Size: 1557 2402
MslI CAynn'nnrTG
Cuts at: 1948 2075 1948
Size: 127 3832
MspA1I CmG'CkG
Cuts at: 1070 1384 1744 2757 3002 1070
Size: 314 360 1013 245 2027
Fragments arranged by size:
2027 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2140 2140
Size: 3959
NarI GG'CG_CC
Cuts at: 1390 1638 1390
Size: 248 3711
NgoMIV G'CCGG_C
Cuts at: 2138 2138
Size: 3959
NspI r_CATG'y
Cuts at: 226 1012 1512 2419 226
Size: 786 500 907 1766
Fragments arranged by size:
1766 907 786 500
PciI A'CATG_T
Cuts at: 2415 2415
Size: 3959
PfoI T'CCnGG_A
Cuts at: 473 473
Size: 3959
PmeI GTTT'AAAC
Cuts at: 958 958
Size: 3959
PmlI CAC'GTG
Cuts at: 230 230
Size: 3959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3098 3130 3098
Size: 32 3927
PshAI GACnn'nnGTC
Cuts at: 763 763
Size: 3959
PstI C_TGCA'G
Cuts at: 553 584 1006 1691 553
Size: 31 422 685 2821
Fragments arranged by size:
2821 685 422 31
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3959
PvuII CAG'CTG
Cuts at: 1384 1744 1384
Size: 360 3599
RsrII CG'GwC_CG
Cuts at: 2154 2154
Size: 3959
SacI G_AGCT'C
Cuts at: 973 973
Size: 3959
SalI G'TCGA_C
Cuts at: 996 996
Size: 3959
SapI GCTCTTCn'nnn_
Cuts at: 1982 2192 1982
Size: 210 3749
SbfI CC_TGCA'GG
Cuts at: 1006 1006
Size: 3959
ScaI AGT'ACT
Cuts at: 3476 3476
Size: 3959
SfcI C'TryA_G
Cuts at: 33 549 580 1002 1687 2680 2871 33
Size: 516 31 422 685 993 191 1121
Fragments arranged by size:
1121 993 685 516 422 191 31
SfoI GGC'GCC
Cuts at: 1391 1639 1391
Size: 248 3711
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3959
SmaI CCC'GGG
Cuts at: 981 981
Size: 3959
SmlI C'TyrA_G
Cuts at: 1114 2029 2521 2783 1114
Size: 915 492 262 2290
Fragments arranged by size:
2290 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3959
SphI G_CATG'C
Cuts at: 1012 1512 1012
Size: 500 3459
SspI AAT'ATT
Cuts at: 314 913 314
Size: 599 3360
StyI C'CwwG_G
Cuts at: 444 1093 1447 444
Size: 649 354 2956
Fragments arranged by size:
2956 649 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1542 1675 2134 2343 1542
Size: 133 459 209 3158
Fragments arranged by size:
3158 459 209 133
TatI w'GTAC_w
Cuts at: 149 3474 149
Size: 3325 634
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 476 508 3476 3508 476
Size: 32 2968 32 927
Fragments arranged by size:
2968 927 32 32
Tth111I GACn'n_nGTC
Cuts at: 1756 1756
Size: 3959
XbaI T'CTAG_A
Cuts at: 990 990
Size: 3959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1454 1454
Size: 3959
XmaI C'CCGG_G
Cuts at: 979 979
Size: 3959
XmnI GAAnn'nnTTC
Cuts at: 743 935 743
Size: 192 3767
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PmlI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I BtgI ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI