Restriction map for
pF1KB6317
(Circular) (Six-base) MAPSORT of: pF1KB6317.seq Check: 2174 from: 1 to: 3893
pF1KB6317 3893 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 931 931
Size: 3893
Acc65I G'GTAC_C
Cuts at: 909 909
Size: 3893
AclI AA'CG_TT
Cuts at: 3733 3733
Size: 3893
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 566 616 1718 2150 2897 3165 269
Size: 297 50 1102 432 747 268 997
Fragments arranged by size:
1102 997 747 432 297 268 50
AflIII A'CryG_T
Cuts at: 1245 2349 3326 1245
Size: 1104 977 1812
Fragments arranged by size:
1812 1104 977
AgeI A'CCGG_T
Cuts at: 2281 2281
Size: 3893
AlwNI CAG_nnn'CTG
Cuts at: 328 1198 2765 328
Size: 870 1567 1456
Fragments arranged by size:
1567 1456 870
ApaLI G'TGCA_C
Cuts at: 2663 2663
Size: 3893
ApoI r'AATT_y
Cuts at: 464 504 897 3246 3502 464
Size: 40 393 2349 256 855
Fragments arranged by size:
2349 855 393 256 40
AseI AT'TA_AT
Cuts at: 20 20
Size: 3893
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3893
AvaI C'yCGr_G
Cuts at: 913 913
Size: 3893
BamHI G'GATC_C
Cuts at: 918 918
Size: 3893
BanI G'GyrC_C
Cuts at: 909 1323 1571 1606 909
Size: 414 248 35 3196
Fragments arranged by size:
3196 414 248 35
BanII G_rGCy'C
Cuts at: 907 1937 907
Size: 1030 2863
BbeI G_GCGC'C
Cuts at: 1327 1575 1327
Size: 248 3645
BciVI GTATCCnnnnn_n'
Cuts at: 247 1791 2552 3893 247
Size: 1544 761 1341 247
Fragments arranged by size:
1544 1341 761 247
BclI T'GATC_A
Cuts at: 1413 1413
Size: 3893
BfrBI ATG'CAT
Cuts at: 368 368
Size: 3893
BglII A'GATC_T
Cuts at: 1408 3445 1408
Size: 2037 1856
BlpI GC'TnA_GC
Cuts at: 1005 1005
Size: 3893
Bme1580I G_kGCm'C
Cuts at: 1518 1611 2667 1518
Size: 93 1056 2744
Fragments arranged by size:
2744 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1105 1186 1521 3665 1105
Size: 81 335 2144 1333
Fragments arranged by size:
2144 1333 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 3893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 413
Size: 3893
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 280 1069 1948 2440 2738 142
Size: 138 789 879 492 298 1297
Fragments arranged by size:
1297 879 789 492 298 138
BsaI GGTCTCn'nnnn_
Cuts at: 3597 3597
Size: 3893
BsaAI yAC'GTr
Cuts at: 1246 1246
Size: 3893
BsaBI GATnn'nnATC
Cuts at: 6 1407 3444 6
Size: 1401 2037 455
Fragments arranged by size:
2037 1401 455
BsaHI Gr'CG_yC
Cuts at: 1324 1572 2274 3770 1324
Size: 248 702 1496 1447
Fragments arranged by size:
1496 1447 702 248
BsaWI w'CCGG_w
Cuts at: 1082 1603 2281 2555 2702 3113 1082
Size: 521 678 274 147 411 1862
Fragments arranged by size:
1862 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 561 1318 2076 2653 3149 561
Size: 757 758 577 496 1305
Fragments arranged by size:
1305 758 757 577 496
BsiEI CG_ry'CG
Cuts at: 104 1435 1481 2689 104
Size: 1331 46 1208 1308
Fragments arranged by size:
1331 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 907 1685 1875 2667 907
Size: 778 190 792 2133
Fragments arranged by size:
2133 792 778 190
BsmI GAATG_Cn'
Cuts at: 370 370
Size: 3893
Bsp1286I G_dGCh'C
Cuts at: 907 1518 1611 1685 1875 1937 2667 907
Size: 611 93 74 190 62 730 2133
Fragments arranged by size:
2133 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3113 3113
Size: 3893
BspHI T'CATG_A
Cuts at: 3069 3069
Size: 3893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 234 943 1157 1459 1840 234
Size: 709 214 302 381 2287
Fragments arranged by size:
2287 709 381 302 214
BsrBI CCG'CTC
Cuts at: 50 150 2185 2239 3723 3888 50
Size: 100 2035 54 1484 165 55
Fragments arranged by size:
2035 1484 165 100 55 54
BsrDI GCAATG_nn'
Cuts at: 1805 1805
Size: 3893
BsrFI r'CCGG_y
Cuts at: 1891 2072 2281 1891
Size: 181 209 3503
Fragments arranged by size:
3503 209 181
BssSI C'ACGA_G
Cuts at: 2164 2522 2164
Size: 358 3535
BstAPI GCAn_nnn'nTGC
Cuts at: 1270 1270
Size: 3893
BstBI TT'CG_AA
Cuts at: 2254 2254
Size: 3893
BstEII G'GTnAC_C
Cuts at: 282 282
Size: 3893
BstXI CCAn_nnnn'nTGG
Cuts at: 391 391
Size: 3893
BstYI r'GATC_y
Cuts at: 1 918 1385 1408 1743 1989 3001 3087 3099
Size: 917 467 23 335 246 1012 86 12
Cuts at: 3099 3445 1
Size: 346 449
Fragments arranged by size:
1012 917 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1843 2024 2187 1843
Size: 181 163 3549
Fragments arranged by size:
3549 181 163
BtsI GCAGTG_nn'
Cuts at: 1136 1136
Size: 3893
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 481 516 481
Size: 35 3858
DraI TTT'AAA
Cuts at: 892 892
Size: 3893
DraIII CAC_nnn'GTG
Cuts at: 207 207
Size: 3893
DrdI GACnn_nn'nnGTC
Cuts at: 1599 2457 1599
Size: 858 3035
EaeI y'GGCC_r
Cuts at: 1478 1652 2043 2070 3415 1478
Size: 174 391 27 1345 1956
Fragments arranged by size:
1956 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1478 1478
Size: 3893
EarI CTCTTCn'nnn_
Cuts at: 1916 2126 1916
Size: 210 3683
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2101 2411 2557 3703 2101
Size: 310 146 1146 2291
Fragments arranged by size:
2291 1146 310 146
EcoICRI GAG'CTC
Cuts at: 905 905
Size: 3893
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 413 566 616 1718 2150 2897 3165 269
Size: 144 153 50 1102 432 747 268 997
Fragments arranged by size:
1102 997 747 432 268 153 144 50
EcoO109I rG'GnC_Cy
Cuts at: 1032 1032
Size: 3893
EcoRI G'AATT_C
Cuts at: 504 897 504
Size: 393 3500
EcoRV GAT'ATC
Cuts at: 582 582
Size: 3893
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 274 1912 274
Size: 1638 2255
FspI TGC'GCA
Cuts at: 1674 1674
Size: 3893
HaeII r_GCGC'y
Cuts at: 1327 1575 2597 3576 1327
Size: 248 1022 979 1644
Fragments arranged by size:
1644 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 276 308 507 539 541 573 628 660 700
Size: 32 199 32 2 32 55 32 40
Cuts at: 700 732 1735 1765 1767 1797 276
Size: 32 1003 30 2 30 2372
Fragments arranged by size:
2372 1003 199 55 40 32 32 32
32 32 30 30 2 2
HincII GTy'rAC
Cuts at: 932 932
Size: 3893
KasI G'GCGC_C
Cuts at: 1323 1571 1323
Size: 248 3645
KpnI G_GTAC'C
Cuts at: 913 913
Size: 3893
MluI A'CGCG_T
Cuts at: 3326 3326
Size: 3893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2564 2748 2564
Size: 184 3709
MscI TGG'CCA
Cuts at: 1654 1654
Size: 3893
MslI CAynn'nnrTG
Cuts at: 1882 2009 1882
Size: 127 3766
MspA1I CmG'CkG
Cuts at: 121 858 1004 1318 1678 2691 2936 121
Size: 737 146 314 360 1013 245 1078
Fragments arranged by size:
1078 1013 737 360 314 245 146
NaeI GCC'GGC
Cuts at: 2074 2074
Size: 3893
NarI GG'CG_CC
Cuts at: 1324 1572 1324
Size: 248 3645
NgoMIV G'CCGG_C
Cuts at: 2072 2072
Size: 3893
NruI TCG'CGA
Cuts at: 146 146
Size: 3893
NsiI A_TGCA'T
Cuts at: 370 370
Size: 3893
NspI r_CATG'y
Cuts at: 946 1446 2353 946
Size: 500 907 2486
Fragments arranged by size:
2486 907 500
PciI A'CATG_T
Cuts at: 2349 2349
Size: 3893
PmeI GTTT'AAAC
Cuts at: 892 892
Size: 3893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3032 3064 3032
Size: 32 3861
PstI C_TGCA'G
Cuts at: 940 1625 940
Size: 685 3208
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3893
PvuII CAG'CTG
Cuts at: 858 1318 1678 858
Size: 460 360 3073
Fragments arranged by size:
3073 460 360
RsrII CG'GwC_CG
Cuts at: 2088 2088
Size: 3893
SacI G_AGCT'C
Cuts at: 907 907
Size: 3893
SalI G'TCGA_C
Cuts at: 930 930
Size: 3893
SapI GCTCTTCn'nnn_
Cuts at: 1916 2126 1916
Size: 210 3683
SbfI CC_TGCA'GG
Cuts at: 940 940
Size: 3893
ScaI AGT'ACT
Cuts at: 3410 3410
Size: 3893
SfcI C'TryA_G
Cuts at: 33 936 1621 2614 2805 33
Size: 903 685 993 191 1121
Fragments arranged by size:
1121 993 903 685 191
SfoI GGC'GCC
Cuts at: 1325 1573 1325
Size: 248 3645
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3893
SmaI CCC'GGG
Cuts at: 915 915
Size: 3893
SmlI C'TyrA_G
Cuts at: 157 259 1048 1963 2455 2717 157
Size: 102 789 915 492 262 1333
Fragments arranged by size:
1333 915 789 492 262 102
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3893
SphI G_CATG'C
Cuts at: 946 1446 946
Size: 500 3393
StyI C'CwwG_G
Cuts at: 1027 1381 1027
Size: 354 3539
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1476 1609 2068 2277 1476
Size: 133 459 209 3092
Fragments arranged by size:
3092 459 209 133
TatI w'GTAC_w
Cuts at: 770 3408 770
Size: 2638 1255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3410 3442 3410
Size: 32 3861
Tth111I GACn'n_nGTC
Cuts at: 1690 1690
Size: 3893
XbaI T'CTAG_A
Cuts at: 924 924
Size: 3893
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1388 1388
Size: 3893
XmaI C'CCGG_G
Cuts at: 913 913
Size: 3893
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NruI NsiI
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BsrGI BssHII BstZ17I Bsu36I BtgI ClaI EcoNI FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI