Restriction map for
pF1KB6315
(Circular) (Six-base) MAPSORT of: pF1KB6315.seq Check: 6276 from: 1 to: 3890
pF1KB6315 3890 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 928 928
Size: 3890
Acc65I G'GTAC_C
Cuts at: 906 906
Size: 3890
AclI AA'CG_TT
Cuts at: 3730 3730
Size: 3890
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 747 1715 2147 2894 3162 747
Size: 968 432 747 268 1475
Fragments arranged by size:
1475 968 747 432 268
AflIII A'CryG_T
Cuts at: 785 1242 2346 3323 785
Size: 457 1104 977 1352
Fragments arranged by size:
1352 1104 977 457
AgeI A'CCGG_T
Cuts at: 2278 2278
Size: 3890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 465 465
Size: 3890
AlwNI CAG_nnn'CTG
Cuts at: 1195 2762 1195
Size: 1567 2323
ApaLI G'TGCA_C
Cuts at: 2660 2660
Size: 3890
ApoI r'AATT_y
Cuts at: 679 700 841 894 3243 3499 679
Size: 21 141 53 2349 256 1070
Fragments arranged by size:
2349 1070 256 141 53 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 3890
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3890
AvaI C'yCGr_G
Cuts at: 910 910
Size: 3890
BamHI G'GATC_C
Cuts at: 915 915
Size: 3890
BanI G'GyrC_C
Cuts at: 906 1320 1568 1603 906
Size: 414 248 35 3193
Fragments arranged by size:
3193 414 248 35
BanII G_rGCy'C
Cuts at: 904 1934 904
Size: 1030 2860
BbeI G_GCGC'C
Cuts at: 1324 1572 1324
Size: 248 3642
BciVI GTATCCnnnnn_n'
Cuts at: 1788 2549 3890 1788
Size: 761 1341 1788
Fragments arranged by size:
1788 1341 761
BclI T'GATC_A
Cuts at: 1410 1410
Size: 3890
BglII A'GATC_T
Cuts at: 627 1405 3442 627
Size: 778 2037 1075
Fragments arranged by size:
2037 1075 778
BlpI GC'TnA_GC
Cuts at: 1002 1002
Size: 3890
Bme1580I G_kGCm'C
Cuts at: 1515 1608 2664 1515
Size: 93 1056 2741
Fragments arranged by size:
2741 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1102 1183 1518 3662 1102
Size: 81 335 2144 1330
Fragments arranged by size:
2144 1330 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 3890
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 1066 1945 2437 2735 317
Size: 749 879 492 298 1472
Fragments arranged by size:
1472 879 749 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3594 3594
Size: 3890
BsaAI yAC'GTr
Cuts at: 1243 1243
Size: 3890
BsaBI GATnn'nnATC
Cuts at: 6 1404 3441 6
Size: 1398 2037 455
Fragments arranged by size:
2037 1398 455
BsaHI Gr'CG_yC
Cuts at: 1321 1569 2271 3767 1321
Size: 248 702 1496 1444
Fragments arranged by size:
1496 1444 702 248
BsaWI w'CCGG_w
Cuts at: 1079 1600 2278 2552 2699 3110 1079
Size: 521 678 274 147 411 1859
Fragments arranged by size:
1859 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 862 1315 2073 2650 3146 862
Size: 453 758 577 496 1606
Fragments arranged by size:
1606 758 577 496 453
BsiEI CG_ry'CG
Cuts at: 104 1432 1478 2686 104
Size: 1328 46 1208 1308
Fragments arranged by size:
1328 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 904 1682 1872 2664 904
Size: 778 190 792 2130
Fragments arranged by size:
2130 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 904 1515 1608 1682 1872 1934 2664 904
Size: 611 93 74 190 62 730 2130
Fragments arranged by size:
2130 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3110 3110
Size: 3890
BspHI T'CATG_A
Cuts at: 3066 3066
Size: 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 940 1154 1456 1837 940
Size: 214 302 381 2993
Fragments arranged by size:
2993 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2182 2236 3720 3885 50
Size: 2132 54 1484 165 55
Fragments arranged by size:
2132 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 641 1802 641
Size: 1161 2729
BsrFI r'CCGG_y
Cuts at: 1888 2069 2278 1888
Size: 181 209 3500
Fragments arranged by size:
3500 209 181
BsrGI T'GTAC_A
Cuts at: 311 311
Size: 3890
BssSI C'ACGA_G
Cuts at: 2161 2519 2161
Size: 358 3532
BstAPI GCAn_nnn'nTGC
Cuts at: 1267 1267
Size: 3890
BstBI TT'CG_AA
Cuts at: 2251 2251
Size: 3890
BstYI r'GATC_y
Cuts at: 1 627 915 1382 1405 1740 1986 2998 3084
Size: 626 288 467 23 335 246 1012 86
Cuts at: 3084 3096 3442 1
Size: 12 346 449
Fragments arranged by size:
1012 626 467 449 346 335 288 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 110 1840 2021 2184 110
Size: 1730 181 163 1816
Fragments arranged by size:
1816 1730 181 163
BtsI GCAGTG_nn'
Cuts at: 1133 1133
Size: 3890
DraI TTT'AAA
Cuts at: 678 889 678
Size: 211 3679
DrdI GACnn_nn'nnGTC
Cuts at: 1596 2454 1596
Size: 858 3032
EaeI y'GGCC_r
Cuts at: 638 1475 1649 2040 2067 3412 638
Size: 837 174 391 27 1345 1116
Fragments arranged by size:
1345 1116 837 391 174 27
EagI C'GGCC_G
Cuts at: 1475 1475
Size: 3890
EarI CTCTTCn'nnn_
Cuts at: 1913 2123 1913
Size: 210 3680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2098 2408 2554 3700 2098
Size: 310 146 1146 2288
Fragments arranged by size:
2288 1146 310 146
EcoICRI GAG'CTC
Cuts at: 902 902
Size: 3890
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 747 1715 2147 2894 3162 747
Size: 968 432 747 268 1475
Fragments arranged by size:
1475 968 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1029 1029
Size: 3890
EcoRI G'AATT_C
Cuts at: 894 894
Size: 3890
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1909 1909
Size: 3890
FspI TGC'GCA
Cuts at: 1671 1671
Size: 3890
HaeII r_GCGC'y
Cuts at: 1324 1572 2594 3573 1324
Size: 248 1022 979 1641
Fragments arranged by size:
1641 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 772 804 1732 1762 1764 1794 772
Size: 32 928 30 2 30 2868
Fragments arranged by size:
2868 928 32 30 30 2
HincII GTy'rAC
Cuts at: 929 929
Size: 3890
KasI G'GCGC_C
Cuts at: 1320 1568 1320
Size: 248 3642
KpnI G_GTAC'C
Cuts at: 910 910
Size: 3890
MluI A'CGCG_T
Cuts at: 3323 3323
Size: 3890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 689 2561 2745 689
Size: 1872 184 1834
Fragments arranged by size:
1872 1834 184
MscI TGG'CCA
Cuts at: 640 1651 640
Size: 1011 2879
MslI CAynn'nnrTG
Cuts at: 409 1879 2006 409
Size: 1470 127 2293
Fragments arranged by size:
2293 1470 127
MspA1I CmG'CkG
Cuts at: 309 1001 1315 1675 2688 2933 309
Size: 692 314 360 1013 245 1266
Fragments arranged by size:
1266 1013 692 360 314 245
NaeI GCC'GGC
Cuts at: 2071 2071
Size: 3890
NarI GG'CG_CC
Cuts at: 1321 1569 1321
Size: 248 3642
NgoMIV G'CCGG_C
Cuts at: 2069 2069
Size: 3890
NspI r_CATG'y
Cuts at: 789 943 1443 2350 789
Size: 154 500 907 2329
Fragments arranged by size:
2329 907 500 154
PciI A'CATG_T
Cuts at: 785 2346 785
Size: 1561 2329
PfoI T'CCnGG_A
Cuts at: 609 609
Size: 3890
PmeI GTTT'AAAC
Cuts at: 889 889
Size: 3890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3029 3061 3029
Size: 32 3858
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 245 277 245
Size: 32 3858
PstI C_TGCA'G
Cuts at: 937 1622 937
Size: 685 3205
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3890
PvuII CAG'CTG
Cuts at: 309 1315 1675 309
Size: 1006 360 2524
Fragments arranged by size:
2524 1006 360
RsrII CG'GwC_CG
Cuts at: 2085 2085
Size: 3890
SacI G_AGCT'C
Cuts at: 904 904
Size: 3890
SalI G'TCGA_C
Cuts at: 927 927
Size: 3890
SapI GCTCTTCn'nnn_
Cuts at: 1913 2123 1913
Size: 210 3680
SbfI CC_TGCA'GG
Cuts at: 937 937
Size: 3890
ScaI AGT'ACT
Cuts at: 3407 3407
Size: 3890
SfcI C'TryA_G
Cuts at: 33 933 1618 2611 2802 33
Size: 900 685 993 191 1121
Fragments arranged by size:
1121 993 900 685 191
SfoI GGC'GCC
Cuts at: 1322 1570 1322
Size: 248 3642
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3890
SmaI CCC'GGG
Cuts at: 912 912
Size: 3890
SmlI C'TyrA_G
Cuts at: 332 1045 1960 2452 2714 332
Size: 713 915 492 262 1508
Fragments arranged by size:
1508 915 713 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3890
SphI G_CATG'C
Cuts at: 943 1443 943
Size: 500 3390
StyI C'CwwG_G
Cuts at: 1024 1378 1024
Size: 354 3536
SwaI ATTT'AAAT
Cuts at: 678 678
Size: 3890
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1473 1606 2065 2274 1473
Size: 133 459 209 3089
Fragments arranged by size:
3089 459 209 133
TatI w'GTAC_w
Cuts at: 311 874 3405 311
Size: 563 2531 796
Fragments arranged by size:
2531 796 563
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3407 3439 3407
Size: 32 3858
Tth111I GACn'n_nGTC
Cuts at: 1687 1687
Size: 3890
XbaI T'CTAG_A
Cuts at: 630 921 630
Size: 291 3599
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 740 1385 740
Size: 645 3245
XmaI C'CCGG_G
Cuts at: 910 910
Size: 3890
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PmeI PpiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI XhoI XmnI ZraI