Restriction map for
pF1KB6314
(Circular) (Six-base) MAPSORT of: pF1KB6314.seq Check: 1605 from: 1 to: 3956
pF1KB6314 3956 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 457 692 740 994 457
Size: 235 48 254 3419
Fragments arranged by size:
3419 254 235 48
Acc65I G'GTAC_C
Cuts at: 972 972
Size: 3956
AclI AA'CG_TT
Cuts at: 3796 3796
Size: 3956
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 1781 2213 2960 3228 340
Size: 1441 432 747 268 1068
Fragments arranged by size:
1441 1068 747 432 268
AflIII A'CryG_T
Cuts at: 1308 2412 3389 1308
Size: 1104 977 1875
Fragments arranged by size:
1875 1104 977
AgeI A'CCGG_T
Cuts at: 2344 2344
Size: 3956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 736 736
Size: 3956
AlwNI CAG_nnn'CTG
Cuts at: 771 1261 2828 771
Size: 490 1567 1899
Fragments arranged by size:
1899 1567 490
ApaI G_GGCC'C
Cuts at: 180 828 180
Size: 648 3308
ApaLI G'TGCA_C
Cuts at: 2726 2726
Size: 3956
ApoI r'AATT_y
Cuts at: 960 3309 3565 960
Size: 2349 256 1351
Fragments arranged by size:
2349 1351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3956
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
AvaI C'yCGr_G
Cuts at: 976 976
Size: 3956
BamHI G'GATC_C
Cuts at: 981 981
Size: 3956
BanI G'GyrC_C
Cuts at: 972 1386 1634 1669 972
Size: 414 248 35 3259
Fragments arranged by size:
3259 414 248 35
BanII G_rGCy'C
Cuts at: 180 828 899 970 2000 180
Size: 648 71 71 1030 2136
Fragments arranged by size:
2136 1030 648 71 71
BbeI G_GCGC'C
Cuts at: 1390 1638 1390
Size: 248 3708
BbsI GAAGACnn'nnnn_
Cuts at: 602 602
Size: 3956
BbvCI CC'TCA_GC
Cuts at: 535 535
Size: 3956
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3922
BciVI GTATCCnnnnn_n'
Cuts at: 770 1854 2615 3956 770
Size: 1084 761 1341 770
Fragments arranged by size:
1341 1084 770 761
BclI T'GATC_A
Cuts at: 653 870 1476 653
Size: 217 606 3133
Fragments arranged by size:
3133 606 217
BglI GCCn_nnn'nGGC
Cuts at: 222 222
Size: 3956
BglII A'GATC_T
Cuts at: 140 1471 3508 140
Size: 1331 2037 588
Fragments arranged by size:
2037 1331 588
BlpI GC'TnA_GC
Cuts at: 1068 1068
Size: 3956
Bme1580I G_kGCm'C
Cuts at: 180 218 828 1581 1674 2730 180
Size: 38 610 753 93 1056 1406
Fragments arranged by size:
1406 1056 753 610 93 38
BmrI ACTGGGnnnn_n'
Cuts at: 156 503 674 821 1168 1249 1584 3728 156
Size: 347 171 147 347 81 335 2144 384
Fragments arranged by size:
2144 384 347 347 335 171 147 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2323 2323
Size: 3956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 644 167
Size: 477 3479
Bpu10I CC'TnA_GC
Cuts at: 535 727 535
Size: 192 3764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 555 1132 2011 2503 2801 555
Size: 577 879 492 298 1710
Fragments arranged by size:
1710 879 577 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3660 3660
Size: 3956
BsaAI yAC'GTr
Cuts at: 1309 1309
Size: 3956
BsaBI GATnn'nnATC
Cuts at: 6 1470 3507 6
Size: 1464 2037 455
Fragments arranged by size:
2037 1464 455
BsaHI Gr'CG_yC
Cuts at: 1387 1635 2337 3833 1387
Size: 248 702 1496 1510
Fragments arranged by size:
1510 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1145 1666 2344 2618 2765 3176 1145
Size: 521 678 274 147 411 1925
Fragments arranged by size:
1925 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1381 2139 2716 3212 1381
Size: 758 577 496 2125
Fragments arranged by size:
2125 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 789 789
Size: 3956
BsiEI CG_ry'CG
Cuts at: 104 1498 1544 2752 104
Size: 1394 46 1208 1308
Fragments arranged by size:
1394 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 899 970 1748 1938 2730 899
Size: 71 778 190 792 2125
Fragments arranged by size:
2125 792 778 190 71
Bsp1286I G_dGCh'C
Cuts at: 180 218 828 899 970 1581 1674 1748 1938
Size: 38 610 71 71 611 93 74 190
Cuts at: 1938 2000 2730 180
Size: 62 730 1406
Fragments arranged by size:
1406 730 611 610 190 93 74 71
71 62 38
BspEI T'CCGG_A
Cuts at: 3176 3176
Size: 3956
BspHI T'CATG_A
Cuts at: 3132 3132
Size: 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 728 1006 1220 1522 1903 728
Size: 278 214 302 381 2781
Fragments arranged by size:
2781 381 302 278 214
BsrBI CCG'CTC
Cuts at: 50 2248 2302 3786 3951 50
Size: 2198 54 1484 165 55
Fragments arranged by size:
2198 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1868 1868
Size: 3956
BsrFI r'CCGG_y
Cuts at: 1954 2135 2344 1954
Size: 181 209 3566
Fragments arranged by size:
3566 209 181
BssSI C'ACGA_G
Cuts at: 2227 2585 2227
Size: 358 3598
BstAPI GCAn_nnn'nTGC
Cuts at: 1333 1333
Size: 3956
BstBI TT'CG_AA
Cuts at: 2317 2317
Size: 3956
BstXI CCAn_nnnn'nTGG
Cuts at: 585 585
Size: 3956
BstYI r'GATC_y
Cuts at: 1 140 768 981 1448 1471 1806 2052 3064
Size: 139 628 213 467 23 335 246 1012
Cuts at: 3064 3150 3162 3508 1
Size: 86 12 346 449
Fragments arranged by size:
1012 628 467 449 346 335 246 213
139 86 23 12
BstZ17I GTA'TAC
Cuts at: 693 693
Size: 3956
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1906 2087 2250 1906
Size: 181 163 3612
Fragments arranged by size:
3612 181 163
BtsI GCAGTG_nn'
Cuts at: 165 292 854 1199 165
Size: 127 562 345 2922
Fragments arranged by size:
2922 562 345 127
DraI TTT'AAA
Cuts at: 955 955
Size: 3956
DrdI GACnn_nn'nnGTC
Cuts at: 1662 2520 1662
Size: 858 3098
EaeI y'GGCC_r
Cuts at: 1541 1715 2106 2133 3478 1541
Size: 174 391 27 1345 2019
Fragments arranged by size:
2019 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1541 1541
Size: 3956
EarI CTCTTCn'nnn_
Cuts at: 319 424 1979 2189 319
Size: 105 1555 210 2086
Fragments arranged by size:
2086 1555 210 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 266 2164 2474 2620 3766 266
Size: 1898 310 146 1146 456
Fragments arranged by size:
1898 1146 456 310 146
EcoICRI GAG'CTC
Cuts at: 897 968 897
Size: 71 3885
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 340 644 1781 2213 2960 3228 167
Size: 173 304 1137 432 747 268 895
Fragments arranged by size:
1137 895 747 432 304 268 173
EcoNI CCTnn'n_nnAGG
Cuts at: 430 430
Size: 3956
EcoO109I rG'GnC_Cy
Cuts at: 177 1095 177
Size: 918 3038
EcoRI G'AATT_C
Cuts at: 960 960
Size: 3956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1975 1975
Size: 3956
FspI TGC'GCA
Cuts at: 1737 1737
Size: 3956
HaeII r_GCGC'y
Cuts at: 1390 1638 2660 3639 1390
Size: 248 1022 979 1707
Fragments arranged by size:
1707 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1798 1828 1830 1860 1798
Size: 30 2 30 3894
Fragments arranged by size:
3894 30 30 2
HincII GTy'rAC
Cuts at: 885 995 885
Size: 110 3846
KasI G'GCGC_C
Cuts at: 1386 1634 1386
Size: 248 3708
KpnI G_GTAC'C
Cuts at: 976 976
Size: 3956
MluI A'CGCG_T
Cuts at: 3389 3389
Size: 3956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 229 590 2627 2811 229
Size: 361 2037 184 1374
Fragments arranged by size:
2037 1374 361 184
MscI TGG'CCA
Cuts at: 1717 1717
Size: 3956
MslI CAynn'nnrTG
Cuts at: 878 1945 2072 878
Size: 1067 127 2762
Fragments arranged by size:
2762 1067 127
MspA1I CmG'CkG
Cuts at: 309 1067 1381 1741 2754 2999 309
Size: 758 314 360 1013 245 1266
Fragments arranged by size:
1266 1013 758 360 314 245
NaeI GCC'GGC
Cuts at: 2137 2137
Size: 3956
NarI GG'CG_CC
Cuts at: 1387 1635 1387
Size: 248 3708
NgoMIV G'CCGG_C
Cuts at: 2135 2135
Size: 3956
NspI r_CATG'y
Cuts at: 1009 1509 2416 1009
Size: 500 907 2549
Fragments arranged by size:
2549 907 500
PasI CC'CwG_GG
Cuts at: 173 173
Size: 3956
PciI A'CATG_T
Cuts at: 2412 2412
Size: 3956
PflMI CCAn_nnn'nTGG
Cuts at: 771 771
Size: 3956
PmeI GTTT'AAAC
Cuts at: 955 955
Size: 3956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3095 3127 3095
Size: 32 3924
PspOMI G'GGCC_C
Cuts at: 176 824 176
Size: 648 3308
PstI C_TGCA'G
Cuts at: 1003 1688 1003
Size: 685 3271
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3956
PvuII CAG'CTG
Cuts at: 1381 1741 1381
Size: 360 3596
RsrII CG'GwC_CG
Cuts at: 2151 2151
Size: 3956
SacI G_AGCT'C
Cuts at: 899 970 899
Size: 71 3885
SalI G'TCGA_C
Cuts at: 993 993
Size: 3956
SapI GCTCTTCn'nnn_
Cuts at: 424 1979 2189 424
Size: 1555 210 2191
Fragments arranged by size:
2191 1555 210
SbfI CC_TGCA'GG
Cuts at: 1003 1003
Size: 3956
ScaI AGT'ACT
Cuts at: 3473 3473
Size: 3956
SfcI C'TryA_G
Cuts at: 33 427 999 1684 2677 2868 33
Size: 394 572 685 993 191 1121
Fragments arranged by size:
1121 993 685 572 394 191
SfoI GGC'GCC
Cuts at: 1388 1636 1388
Size: 248 3708
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
SmaI CCC'GGG
Cuts at: 978 978
Size: 3956
SmlI C'TyrA_G
Cuts at: 570 1111 2026 2518 2780 570
Size: 541 915 492 262 1746
Fragments arranged by size:
1746 915 541 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3956
SphI G_CATG'C
Cuts at: 1009 1509 1009
Size: 500 3456
StuI AGG'CCT
Cuts at: 225 347 225
Size: 122 3834
StyI C'CwwG_G
Cuts at: 386 1090 1444 386
Size: 704 354 2898
Fragments arranged by size:
2898 704 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 138 1539 1672 2131 2340 138
Size: 1401 133 459 209 1754
Fragments arranged by size:
1754 1401 459 209 133
TatI w'GTAC_w
Cuts at: 671 3471 671
Size: 2800 1156
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 180 212 3473 3505 180
Size: 32 3261 32 631
Fragments arranged by size:
3261 631 32 32
Tth111I GACn'n_nGTC
Cuts at: 1753 1753
Size: 3956
XbaI T'CTAG_A
Cuts at: 987 987
Size: 3956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1451 1451
Size: 3956
XmaI C'CCGG_G
Cuts at: 976 976
Size: 3956
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BfrBI BmgBI BmtI BsaXI BseRI BsiWI
BsmI BsmBI BsrGI BssHII BstEII Bsu36I BtgI ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI