Restriction map for
pF1KB6312
(Circular) (Six-base) MAPSORT of: pF1KB6312.seq Check: 8349 from: 1 to: 3953
pF1KB6312 3953 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 991 991
Size: 3953
Acc65I G'GTAC_C
Cuts at: 969 969
Size: 3953
AclI AA'CG_TT
Cuts at: 3793 3793
Size: 3953
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1778 2210 2957 3225 1778
Size: 432 747 268 2506
Fragments arranged by size:
2506 747 432 268
AflIII A'CryG_T
Cuts at: 1305 2409 3386 1305
Size: 1104 977 1872
Fragments arranged by size:
1872 1104 977
AgeI A'CCGG_T
Cuts at: 2341 2341
Size: 3953
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 875 875
Size: 3953
AlwNI CAG_nnn'CTG
Cuts at: 354 1258 2825 354
Size: 904 1567 1482
Fragments arranged by size:
1567 1482 904
ApaLI G'TGCA_C
Cuts at: 2723 2723
Size: 3953
ApoI r'AATT_y
Cuts at: 957 3306 3562 957
Size: 2349 256 1348
Fragments arranged by size:
2349 1348 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3953
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
AvaI C'yCGr_G
Cuts at: 973 973
Size: 3953
AvrII C'CTAG_G
Cuts at: 115 115
Size: 3953
BamHI G'GATC_C
Cuts at: 870 978 870
Size: 108 3845
BanI G'GyrC_C
Cuts at: 969 1383 1631 1666 969
Size: 414 248 35 3256
Fragments arranged by size:
3256 414 248 35
BanII G_rGCy'C
Cuts at: 967 1997 967
Size: 1030 2923
BbeI G_GCGC'C
Cuts at: 1387 1635 1387
Size: 248 3705
BbsI GAAGACnn'nnnn_
Cuts at: 687 687
Size: 3953
BciVI GTATCCnnnnn_n'
Cuts at: 1851 2612 3953 1851
Size: 761 1341 1851
Fragments arranged by size:
1851 1341 761
BclI T'GATC_A
Cuts at: 1473 1473
Size: 3953
BglII A'GATC_T
Cuts at: 785 1468 3505 785
Size: 683 2037 1233
Fragments arranged by size:
2037 1233 683
BlpI GC'TnA_GC
Cuts at: 1065 1065
Size: 3953
Bme1580I G_kGCm'C
Cuts at: 1578 1671 2727 1578
Size: 93 1056 2804
Fragments arranged by size:
2804 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1165 1246 1581 3725 1165
Size: 81 335 2144 1393
Fragments arranged by size:
2144 1393 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 3953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 647 704 647
Size: 57 3896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 672 1129 2008 2500 2798 232
Size: 440 457 879 492 298 1387
Fragments arranged by size:
1387 879 492 457 440 298
BsaI GGTCTCn'nnnn_
Cuts at: 3657 3657
Size: 3953
BsaAI yAC'GTr
Cuts at: 1306 1306
Size: 3953
BsaBI GATnn'nnATC
Cuts at: 6 1467 3504 6
Size: 1461 2037 455
Fragments arranged by size:
2037 1461 455
BsaHI Gr'CG_yC
Cuts at: 1384 1632 2334 3830 1384
Size: 248 702 1496 1507
Fragments arranged by size:
1507 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1142 1663 2341 2615 2762 3173 1142
Size: 521 678 274 147 411 1922
Fragments arranged by size:
1922 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 235
Size: 3953
BseYI C'CCAG_C
Cuts at: 621 863 1378 2136 2713 3209 621
Size: 242 515 758 577 496 1365
Fragments arranged by size:
1365 758 577 515 496 242
BsiEI CG_ry'CG
Cuts at: 104 195 1495 1541 2749 104
Size: 91 1300 46 1208 1308
Fragments arranged by size:
1308 1300 1208 91 46
BsiHKAI G_wGCw'C
Cuts at: 967 1745 1935 2727 967
Size: 778 190 792 2193
Fragments arranged by size:
2193 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 916 916
Size: 3953
Bsp1286I G_dGCh'C
Cuts at: 967 1578 1671 1745 1935 1997 2727 967
Size: 611 93 74 190 62 730 2193
Fragments arranged by size:
2193 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3173 3173
Size: 3953
BspHI T'CATG_A
Cuts at: 3129 3129
Size: 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1003 1217 1519 1900 1003
Size: 214 302 381 3056
Fragments arranged by size:
3056 381 302 214
BsrBI CCG'CTC
Cuts at: 50 735 2245 2299 3783 3948 50
Size: 685 1510 54 1484 165 55
Fragments arranged by size:
1510 1484 685 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1865 1865
Size: 3953
BsrFI r'CCGG_y
Cuts at: 1951 2132 2341 1951
Size: 181 209 3563
Fragments arranged by size:
3563 209 181
BssSI C'ACGA_G
Cuts at: 2224 2582 2224
Size: 358 3595
BstAPI GCAn_nnn'nTGC
Cuts at: 1330 1330
Size: 3953
BstBI TT'CG_AA
Cuts at: 2314 2314
Size: 3953
BstYI r'GATC_y
Cuts at: 1 278 785 870 978 1445 1468 1803 2049
Size: 277 507 85 108 467 23 335 246
Cuts at: 2049 3061 3147 3159 3505 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 507 467 449 346 335 277 246
108 86 85 23 12
BtgI C'CryG_G
Cuts at: 107 626 647 107
Size: 519 21 3413
Fragments arranged by size:
3413 519 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1903 2084 2247 1903
Size: 181 163 3609
Fragments arranged by size:
3609 181 163
BtsI GCAGTG_nn'
Cuts at: 1196 1196
Size: 3953
DraI TTT'AAA
Cuts at: 952 952
Size: 3953
DrdI GACnn_nn'nnGTC
Cuts at: 691 1659 2517 691
Size: 968 858 2127
Fragments arranged by size:
2127 968 858
EaeI y'GGCC_r
Cuts at: 137 833 1538 1712 2103 2130 3475 137
Size: 696 705 174 391 27 1345 615
Fragments arranged by size:
1345 705 696 615 391 174 27
EagI C'GGCC_G
Cuts at: 1538 1538
Size: 3953
EarI CTCTTCn'nnn_
Cuts at: 353 476 550 1976 2186 353
Size: 123 74 1426 210 2120
Fragments arranged by size:
2120 1426 210 123 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 177 827 2161 2471 2617 3763 177
Size: 650 1334 310 146 1146 367
Fragments arranged by size:
1334 1146 650 367 310 146
EcoICRI GAG'CTC
Cuts at: 965 965
Size: 3953
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 647 704 1778 2210 2957 3225 647
Size: 57 1074 432 747 268 1375
Fragments arranged by size:
1375 1074 747 432 268 57
EcoNI CCTnn'n_nnAGG
Cuts at: 156 247 250 358 156
Size: 91 3 108 3751
Fragments arranged by size:
3751 108 91 3
EcoO109I rG'GnC_Cy
Cuts at: 351 1092 351
Size: 741 3212
EcoRI G'AATT_C
Cuts at: 957 957
Size: 3953
EcoRV GAT'ATC
Cuts at: 384 384
Size: 3953
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1972 1972
Size: 3953
FspI TGC'GCA
Cuts at: 1734 1734
Size: 3953
HaeII r_GCGC'y
Cuts at: 1387 1635 2657 3636 1387
Size: 248 1022 979 1704
Fragments arranged by size:
1704 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1795 1825 1827 1857 1795
Size: 30 2 30 3891
Fragments arranged by size:
3891 30 30 2
HincII GTy'rAC
Cuts at: 992 992
Size: 3953
KasI G'GCGC_C
Cuts at: 1383 1631 1383
Size: 248 3705
KpnI G_GTAC'C
Cuts at: 973 973
Size: 3953
MluI A'CGCG_T
Cuts at: 3386 3386
Size: 3953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 929 2624 2808 929
Size: 1695 184 2074
Fragments arranged by size:
2074 1695 184
MscI TGG'CCA
Cuts at: 139 835 1714 139
Size: 696 879 2378
Fragments arranged by size:
2378 879 696
MslI CAynn'nnrTG
Cuts at: 1942 2069 1942
Size: 127 3826
MspA1I CmG'CkG
Cuts at: 373 867 1064 1378 1738 2751 2996 373
Size: 494 197 314 360 1013 245 1330
Fragments arranged by size:
1330 1013 494 360 314 245 197
NaeI GCC'GGC
Cuts at: 2134 2134
Size: 3953
NarI GG'CG_CC
Cuts at: 1384 1632 1384
Size: 248 3705
NcoI C'CATG_G
Cuts at: 107 647 107
Size: 540 3413
NgoMIV G'CCGG_C
Cuts at: 2132 2132
Size: 3953
NspI r_CATG'y
Cuts at: 1006 1506 2413 1006
Size: 500 907 2546
Fragments arranged by size:
2546 907 500
PciI A'CATG_T
Cuts at: 2409 2409
Size: 3953
PmeI GTTT'AAAC
Cuts at: 952 952
Size: 3953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 3921
PstI C_TGCA'G
Cuts at: 928 1000 1685 928
Size: 72 685 3196
Fragments arranged by size:
3196 685 72
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3953
PvuII CAG'CTG
Cuts at: 373 867 1378 1738 373
Size: 494 511 360 2588
Fragments arranged by size:
2588 511 494 360
RsrII CG'GwC_CG
Cuts at: 2148 2148
Size: 3953
SacI G_AGCT'C
Cuts at: 967 967
Size: 3953
SalI G'TCGA_C
Cuts at: 990 990
Size: 3953
SapI GCTCTTCn'nnn_
Cuts at: 1976 2186 1976
Size: 210 3743
SbfI CC_TGCA'GG
Cuts at: 1000 1000
Size: 3953
ScaI AGT'ACT
Cuts at: 3470 3470
Size: 3953
SfcI C'TryA_G
Cuts at: 33 924 996 1681 2674 2865 33
Size: 891 72 685 993 191 1121
Fragments arranged by size:
1121 993 891 685 191 72
SfoI GGC'GCC
Cuts at: 1385 1633 1385
Size: 248 3705
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
SmaI CCC'GGG
Cuts at: 975 975
Size: 3953
SmlI C'TyrA_G
Cuts at: 247 687 1108 2023 2515 2777 247
Size: 440 421 915 492 262 1423
Fragments arranged by size:
1423 915 492 440 421 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3953
SphI G_CATG'C
Cuts at: 1006 1506 1006
Size: 500 3453
StuI AGG'CCT
Cuts at: 668 880 668
Size: 212 3741
StyI C'CwwG_G
Cuts at: 107 115 293 333 647 881 1087 1441 107
Size: 8 178 40 314 234 206 354 2619
Fragments arranged by size:
2619 354 314 234 206 178 40 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 690 1536 1669 2128 2337 690
Size: 846 133 459 209 2306
Fragments arranged by size:
2306 846 459 209 133
TatI w'GTAC_w
Cuts at: 3468 3468
Size: 3953
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 3921
Tth111I GACn'n_nGTC
Cuts at: 1750 1750
Size: 3953
XbaI T'CTAG_A
Cuts at: 984 984
Size: 3953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1448 1448
Size: 3953
XmaI C'CCGG_G
Cuts at: 973 973
Size: 3953
XmnI GAAnn'nnTTC
Cuts at: 187 416 187
Size: 229 3724
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI