Restriction map for
pF1KB6307
(Circular) (Six-base) MAPSORT of: pF1KB6307.seq Check: 4081 from: 1 to: 3887
pF1KB6307 3887 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 925 925
Size: 3887
Acc65I G'GTAC_C
Cuts at: 903 903
Size: 3887
AclI AA'CG_TT
Cuts at: 3727 3727
Size: 3887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 474 680 1712 2144 2891 3159 474
Size: 206 1032 432 747 268 1202
Fragments arranged by size:
1202 1032 747 432 268 206
AflIII A'CryG_T
Cuts at: 1239 2343 3320 1239
Size: 1104 977 1806
Fragments arranged by size:
1806 1104 977
AgeI A'CCGG_T
Cuts at: 2275 2275
Size: 3887
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 118 150 118
Size: 32 3855
AlwNI CAG_nnn'CTG
Cuts at: 658 1192 2759 658
Size: 534 1567 1786
Fragments arranged by size:
1786 1567 534
ApaLI G'TGCA_C
Cuts at: 2657 2657
Size: 3887
ApoI r'AATT_y
Cuts at: 433 891 3240 3496 433
Size: 458 2349 256 824
Fragments arranged by size:
2349 824 458 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3887
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
AvaI C'yCGr_G
Cuts at: 907 907
Size: 3887
BamHI G'GATC_C
Cuts at: 912 912
Size: 3887
BanI G'GyrC_C
Cuts at: 153 162 238 537 903 1317 1565 1600 153
Size: 9 76 299 366 414 248 35 2440
Fragments arranged by size:
2440 414 366 299 248 76 35 9
BanII G_rGCy'C
Cuts at: 505 901 1931 505
Size: 396 1030 2461
Fragments arranged by size:
2461 1030 396
BbeI G_GCGC'C
Cuts at: 157 1321 1569 157
Size: 1164 248 2475
Fragments arranged by size:
2475 1164 248
BbvCI CC'TCA_GC
Cuts at: 445 445
Size: 3887
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3853
BciVI GTATCCnnnnn_n'
Cuts at: 1785 2546 3887 1785
Size: 761 1341 1785
Fragments arranged by size:
1785 1341 761
BclI T'GATC_A
Cuts at: 1407 1407
Size: 3887
BglII A'GATC_T
Cuts at: 1402 3439 1402
Size: 2037 1850
BlpI GC'TnA_GC
Cuts at: 671 999 671
Size: 328 3559
Bme1580I G_kGCm'C
Cuts at: 1512 1605 2661 1512
Size: 93 1056 2738
Fragments arranged by size:
2738 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1099 1180 1515 3659 1099
Size: 81 335 2144 1327
Fragments arranged by size:
2144 1327 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2254 2254
Size: 3887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 809 809
Size: 3887
Bpu10I CC'TnA_GC
Cuts at: 445 445
Size: 3887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1942 2434 2732 1063
Size: 879 492 298 2218
Fragments arranged by size:
2218 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3591 3591
Size: 3887
BsaAI yAC'GTr
Cuts at: 1240 1240
Size: 3887
BsaBI GATnn'nnATC
Cuts at: 6 1401 3438 6
Size: 1395 2037 455
Fragments arranged by size:
2037 1395 455
BsaHI Gr'CG_yC
Cuts at: 154 254 1318 1566 2268 3764 154
Size: 100 1064 248 702 1496 277
Fragments arranged by size:
1496 1064 702 277 248 100
BsaWI w'CCGG_w
Cuts at: 159 1076 1597 2275 2549 2696 3107 159
Size: 917 521 678 274 147 411 939
Fragments arranged by size:
939 917 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 349 379 349
Size: 30 3857
BseYI C'CCAG_C
Cuts at: 1312 2070 2647 3143 1312
Size: 758 577 496 2056
Fragments arranged by size:
2056 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 144 1429 1475 2683 104
Size: 40 1285 46 1208 1308
Fragments arranged by size:
1308 1285 1208 46 40
BsiHKAI G_wGCw'C
Cuts at: 901 1679 1869 2661 901
Size: 778 190 792 2127
Fragments arranged by size:
2127 792 778 190
BsmI GAATG_Cn'
Cuts at: 773 773
Size: 3887
Bsp1286I G_dGCh'C
Cuts at: 505 901 1512 1605 1679 1869 1931 2661 505
Size: 396 611 93 74 190 62 730 1731
Fragments arranged by size:
1731 730 611 396 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3107 3107
Size: 3887
BspHI T'CATG_A
Cuts at: 3063 3063
Size: 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 937 1151 1453 1834 937
Size: 214 302 381 2990
Fragments arranged by size:
2990 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2179 2233 3717 3882 50
Size: 2129 54 1484 165 55
Fragments arranged by size:
2129 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1799 1799
Size: 3887
BsrFI r'CCGG_y
Cuts at: 1885 2066 2275 1885
Size: 181 209 3497
Fragments arranged by size:
3497 209 181
BssSI C'ACGA_G
Cuts at: 2158 2516 2158
Size: 358 3529
BstAPI GCAn_nnn'nTGC
Cuts at: 1264 1264
Size: 3887
BstBI TT'CG_AA
Cuts at: 2248 2248
Size: 3887
BstYI r'GATC_y
Cuts at: 1 597 912 1379 1402 1737 1983 2995 3081
Size: 596 315 467 23 335 246 1012 86
Cuts at: 3081 3093 3439 1
Size: 12 346 449
Fragments arranged by size:
1012 596 467 449 346 335 315 246
86 23 12
BtgI C'CryG_G
Cuts at: 368 368
Size: 3887
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1837 2018 2181 1837
Size: 181 163 3543
Fragments arranged by size:
3543 181 163
BtsI GCAGTG_nn'
Cuts at: 416 1130 416
Size: 714 3173
DraI TTT'AAA
Cuts at: 886 886
Size: 3887
DrdI GACnn_nn'nnGTC
Cuts at: 1593 2451 1593
Size: 858 3029
EaeI y'GGCC_r
Cuts at: 1472 1646 2037 2064 3409 1472
Size: 174 391 27 1345 1950
Fragments arranged by size:
1950 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1472 1472
Size: 3887
EarI CTCTTCn'nnn_
Cuts at: 656 1910 2120 656
Size: 1254 210 2423
Fragments arranged by size:
2423 1254 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2095 2405 2551 3697 2095
Size: 310 146 1146 2285
Fragments arranged by size:
2285 1146 310 146
EcoICRI GAG'CTC
Cuts at: 899 899
Size: 3887
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 474 680 809 1712 2144 2891 3159 474
Size: 206 129 903 432 747 268 1202
Fragments arranged by size:
1202 903 747 432 268 206 129
EcoNI CCTnn'n_nnAGG
Cuts at: 742 742
Size: 3887
EcoO109I rG'GnC_Cy
Cuts at: 126 1026 126
Size: 900 2987
EcoRI G'AATT_C
Cuts at: 891 891
Size: 3887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1906 1906
Size: 3887
FspI TGC'GCA
Cuts at: 1668 1668
Size: 3887
HaeII r_GCGC'y
Cuts at: 157 1321 1569 2591 3570 157
Size: 1164 248 1022 979 474
Fragments arranged by size:
1164 1022 979 474 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 349 381 392 424 596 628 1729 1759 1761
Size: 32 11 32 172 32 1101 30 2
Cuts at: 1761 1791 349
Size: 30 2445
Fragments arranged by size:
2445 1101 172 32 32 32 30 30
11 2
HincII GTy'rAC
Cuts at: 926 926
Size: 3887
KasI G'GCGC_C
Cuts at: 153 1317 1565 153
Size: 1164 248 2475
Fragments arranged by size:
2475 1164 248
KpnI G_GTAC'C
Cuts at: 907 907
Size: 3887
MluI A'CGCG_T
Cuts at: 3320 3320
Size: 3887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 429 564 575 611 2558 2742 429
Size: 135 11 36 1947 184 1574
Fragments arranged by size:
1947 1574 184 135 36 11
MscI TGG'CCA
Cuts at: 1648 1648
Size: 3887
MslI CAynn'nnrTG
Cuts at: 1876 2003 1876
Size: 127 3760
MspA1I CmG'CkG
Cuts at: 146 370 998 1312 1672 2685 2930 146
Size: 224 628 314 360 1013 245 1103
Fragments arranged by size:
1103 1013 628 360 314 245 224
NaeI GCC'GGC
Cuts at: 2068 2068
Size: 3887
NarI GG'CG_CC
Cuts at: 154 1318 1566 154
Size: 1164 248 2475
Fragments arranged by size:
2475 1164 248
NgoMIV G'CCGG_C
Cuts at: 2066 2066
Size: 3887
NspI r_CATG'y
Cuts at: 940 1440 2347 940
Size: 500 907 2480
Fragments arranged by size:
2480 907 500
PciI A'CATG_T
Cuts at: 2343 2343
Size: 3887
PflMI CCAn_nnn'nTGG
Cuts at: 854 854
Size: 3887
PmeI GTTT'AAAC
Cuts at: 886 886
Size: 3887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3026 3058 3026
Size: 32 3855
PpuMI rG'GwC_Cy
Cuts at: 126 126
Size: 3887
PstI C_TGCA'G
Cuts at: 934 1619 934
Size: 685 3202
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3887
PvuII CAG'CTG
Cuts at: 1312 1672 1312
Size: 360 3527
RsrII CG'GwC_CG
Cuts at: 2082 2082
Size: 3887
SacI G_AGCT'C
Cuts at: 901 901
Size: 3887
SacII CC_GC'GG
Cuts at: 371 371
Size: 3887
SalI G'TCGA_C
Cuts at: 924 924
Size: 3887
SanDI GG'GwC_CC
Cuts at: 126 126
Size: 3887
SapI GCTCTTCn'nnn_
Cuts at: 1910 2120 1910
Size: 210 3677
SbfI CC_TGCA'GG
Cuts at: 934 934
Size: 3887
ScaI AGT'ACT
Cuts at: 868 3404 868
Size: 2536 1351
SfcI C'TryA_G
Cuts at: 33 930 1615 2608 2799 33
Size: 897 685 993 191 1121
Fragments arranged by size:
1121 993 897 685 191
SfoI GGC'GCC
Cuts at: 155 1319 1567 155
Size: 1164 248 2475
Fragments arranged by size:
2475 1164 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
SmaI CCC'GGG
Cuts at: 909 909
Size: 3887
SmlI C'TyrA_G
Cuts at: 1042 1957 2449 2711 1042
Size: 915 492 262 2218
Fragments arranged by size:
2218 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3887
SphI G_CATG'C
Cuts at: 940 1440 940
Size: 500 3387
StuI AGG'CCT
Cuts at: 450 450
Size: 3887
StyI C'CwwG_G
Cuts at: 1021 1375 1021
Size: 354 3533
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 525 1470 1603 2062 2271 525
Size: 945 133 459 209 2141
Fragments arranged by size:
2141 945 459 209 133
TatI w'GTAC_w
Cuts at: 338 866 3402 338
Size: 528 2536 823
Fragments arranged by size:
2536 823 528
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3404 3436 3404
Size: 32 3855
Tth111I GACn'n_nGTC
Cuts at: 1684 1684
Size: 3887
XbaI T'CTAG_A
Cuts at: 918 918
Size: 3887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1382 1382
Size: 3887
XmaI C'CCGG_G
Cuts at: 907 907
Size: 3887
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BfrBI BglI BmgBI BmtI
BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI