Restriction map for
pF1KB6304
(Circular) (Six-base) MAPSORT of: pF1KB6304.seq Check: 1377 from: 1 to: 3947
pF1KB6304 3947 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 260 576 613 926 985 260
Size: 316 37 313 59 3222
Fragments arranged by size:
3222 316 313 59 37
Acc65I G'GTAC_C
Cuts at: 963 963
Size: 3947
AclI AA'CG_TT
Cuts at: 3787 3787
Size: 3947
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1772 2204 2951 3219 1772
Size: 432 747 268 2500
Fragments arranged by size:
2500 747 432 268
AflIII A'CryG_T
Cuts at: 1299 2403 3380 1299
Size: 1104 977 1866
Fragments arranged by size:
1866 1104 977
AgeI A'CCGG_T
Cuts at: 2335 2335
Size: 3947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 158 158
Size: 3947
AlwNI CAG_nnn'CTG
Cuts at: 1252 2819 1252
Size: 1567 2380
ApaLI G'TGCA_C
Cuts at: 2717 2717
Size: 3947
ApoI r'AATT_y
Cuts at: 348 543 679 951 3300 3556 348
Size: 195 136 272 2349 256 739
Fragments arranged by size:
2349 739 272 256 195 136
AseI AT'TA_AT
Cuts at: 20 20
Size: 3947
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3947
AvaI C'yCGr_G
Cuts at: 967 967
Size: 3947
BamHI G'GATC_C
Cuts at: 972 972
Size: 3947
BanI G'GyrC_C
Cuts at: 963 1377 1625 1660 963
Size: 414 248 35 3250
Fragments arranged by size:
3250 414 248 35
BanII G_rGCy'C
Cuts at: 961 1991 961
Size: 1030 2917
BbeI G_GCGC'C
Cuts at: 1381 1629 1381
Size: 248 3699
BciVI GTATCCnnnnn_n'
Cuts at: 426 1845 2606 3947 426
Size: 1419 761 1341 426
Fragments arranged by size:
1419 1341 761 426
BclI T'GATC_A
Cuts at: 1467 1467
Size: 3947
BglII A'GATC_T
Cuts at: 1462 3499 1462
Size: 2037 1910
BlpI GC'TnA_GC
Cuts at: 1059 1059
Size: 3947
Bme1580I G_kGCm'C
Cuts at: 1572 1665 2721 1572
Size: 93 1056 2798
Fragments arranged by size:
2798 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1159 1240 1575 3719 1159
Size: 81 335 2144 1387
Fragments arranged by size:
2144 1387 335 81
BmtI G_CTAG'C
Cuts at: 445 445
Size: 3947
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 3947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 845 1123 2002 2494 2792 845
Size: 278 879 492 298 2000
Fragments arranged by size:
2000 879 492 298 278
BsaI GGTCTCn'nnnn_
Cuts at: 3651 3651
Size: 3947
BsaAI yAC'GTr
Cuts at: 1300 1300
Size: 3947
BsaBI GATnn'nnATC
Cuts at: 6 1461 3498 6
Size: 1455 2037 455
Fragments arranged by size:
2037 1455 455
BsaHI Gr'CG_yC
Cuts at: 1378 1626 2328 3824 1378
Size: 248 702 1496 1501
Fragments arranged by size:
1501 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1136 1657 2335 2609 2756 3167 1136
Size: 521 678 274 147 411 1916
Fragments arranged by size:
1916 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 721 136
Size: 585 3362
BseYI C'CCAG_C
Cuts at: 1372 2130 2707 3203 1372
Size: 758 577 496 2116
Fragments arranged by size:
2116 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1489 1535 2743 104
Size: 1385 46 1208 1308
Fragments arranged by size:
1385 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 961 1739 1929 2721 961
Size: 778 190 792 2187
Fragments arranged by size:
2187 792 778 190
BsmI GAATG_Cn'
Cuts at: 399 399
Size: 3947
BsmBI CGTCTCn'nnnn_
Cuts at: 857 857
Size: 3947
Bsp1286I G_dGCh'C
Cuts at: 961 1572 1665 1739 1929 1991 2721 961
Size: 611 93 74 190 62 730 2187
Fragments arranged by size:
2187 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3167 3167
Size: 3947
BspHI T'CATG_A
Cuts at: 3123 3123
Size: 3947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 126 997 1211 1513 1894 126
Size: 871 214 302 381 2179
Fragments arranged by size:
2179 871 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2239 2293 3777 3942 50
Size: 2189 54 1484 165 55
Fragments arranged by size:
2189 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1859 1859
Size: 3947
BsrFI r'CCGG_y
Cuts at: 1945 2126 2335 1945
Size: 181 209 3557
Fragments arranged by size:
3557 209 181
BssSI C'ACGA_G
Cuts at: 2218 2576 2218
Size: 358 3589
BstAPI GCAn_nnn'nTGC
Cuts at: 1324 1324
Size: 3947
BstBI TT'CG_AA
Cuts at: 2308 2308
Size: 3947
BstXI CCAn_nnnn'nTGG
Cuts at: 729 729
Size: 3947
BstYI r'GATC_y
Cuts at: 1 972 1439 1462 1797 2043 3055 3141 3153
Size: 971 467 23 335 246 1012 86 12
Cuts at: 3153 3499 1
Size: 346 449
Fragments arranged by size:
1012 971 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 199 107
Size: 92 3855
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1897 2078 2241 1897
Size: 181 163 3603
Fragments arranged by size:
3603 181 163
BtsI GCAGTG_nn'
Cuts at: 467 1190 467
Size: 723 3224
DraI TTT'AAA
Cuts at: 946 946
Size: 3947
DrdI GACnn_nn'nnGTC
Cuts at: 256 1653 2511 256
Size: 1397 858 1692
Fragments arranged by size:
1692 1397 858
EaeI y'GGCC_r
Cuts at: 1532 1706 2097 2124 3469 1532
Size: 174 391 27 1345 2010
Fragments arranged by size:
2010 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1532 1532
Size: 3947
EarI CTCTTCn'nnn_
Cuts at: 915 1970 2180 915
Size: 1055 210 2682
Fragments arranged by size:
2682 1055 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2155 2465 2611 3757 2155
Size: 310 146 1146 2345
Fragments arranged by size:
2345 1146 310 146
EcoICRI GAG'CTC
Cuts at: 959 959
Size: 3947
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1772 2204 2951 3219 1772
Size: 432 747 268 2500
Fragments arranged by size:
2500 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1086 1086
Size: 3947
EcoRI G'AATT_C
Cuts at: 679 951 679
Size: 272 3675
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 928 1966 928
Size: 1038 2909
FspI TGC'GCA
Cuts at: 1728 1728
Size: 3947
HaeII r_GCGC'y
Cuts at: 1381 1629 2651 3630 1381
Size: 248 1022 979 1698
Fragments arranged by size:
1698 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 204 236 460 465 492 497 677 709 1789
Size: 32 224 5 27 5 180 32 1080
Cuts at: 1789 1819 1821 1851 204
Size: 30 2 30 2300
Fragments arranged by size:
2300 1080 224 180 32 32 30 30
27 5 5 2
HincII GTy'rAC
Cuts at: 405 986 405
Size: 581 3366
KasI G'GCGC_C
Cuts at: 1377 1625 1377
Size: 248 3699
KpnI G_GTAC'C
Cuts at: 967 967
Size: 3947
MluI A'CGCG_T
Cuts at: 3380 3380
Size: 3947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2618 2802 2618
Size: 184 3763
MscI TGG'CCA
Cuts at: 1708 1708
Size: 3947
MslI CAynn'nnrTG
Cuts at: 1936 2063 1936
Size: 127 3820
MspA1I CmG'CkG
Cuts at: 1058 1372 1732 2745 2990 1058
Size: 314 360 1013 245 2015
Fragments arranged by size:
2015 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2128 2128
Size: 3947
NarI GG'CG_CC
Cuts at: 1378 1626 1378
Size: 248 3699
NcoI C'CATG_G
Cuts at: 107 107
Size: 3947
NgoMIV G'CCGG_C
Cuts at: 2126 2126
Size: 3947
NheI G'CTAG_C
Cuts at: 441 441
Size: 3947
NspI r_CATG'y
Cuts at: 1000 1500 2407 1000
Size: 500 907 2540
Fragments arranged by size:
2540 907 500
PciI A'CATG_T
Cuts at: 2403 2403
Size: 3947
PfoI T'CCnGG_A
Cuts at: 245 245
Size: 3947
PmeI GTTT'AAAC
Cuts at: 946 946
Size: 3947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3086 3118 3086
Size: 32 3915
PstI C_TGCA'G
Cuts at: 462 705 994 1679 462
Size: 243 289 685 2730
Fragments arranged by size:
2730 685 289 243
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3947
PvuII CAG'CTG
Cuts at: 1372 1732 1372
Size: 360 3587
RsrII CG'GwC_CG
Cuts at: 2142 2142
Size: 3947
SacI G_AGCT'C
Cuts at: 961 961
Size: 3947
SalI G'TCGA_C
Cuts at: 984 984
Size: 3947
SapI GCTCTTCn'nnn_
Cuts at: 1970 2180 1970
Size: 210 3737
SbfI CC_TGCA'GG
Cuts at: 994 994
Size: 3947
ScaI AGT'ACT
Cuts at: 3464 3464
Size: 3947
SexAI A'CCwGG_T
Cuts at: 263 263
Size: 3947
SfcI C'TryA_G
Cuts at: 33 458 614 701 990 1675 2668 2859 33
Size: 425 156 87 289 685 993 191 1121
Fragments arranged by size:
1121 993 685 425 289 191 156 87
SfoI GGC'GCC
Cuts at: 1379 1627 1379
Size: 248 3699
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3947
SmaI CCC'GGG
Cuts at: 969 969
Size: 3947
SmlI C'TyrA_G
Cuts at: 860 1102 2017 2509 2771 860
Size: 242 915 492 262 2036
Fragments arranged by size:
2036 915 492 262 242
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3947
SphI G_CATG'C
Cuts at: 1000 1500 1000
Size: 500 3447
SspI AAT'ATT
Cuts at: 161 161
Size: 3947
StyI C'CwwG_G
Cuts at: 107 1081 1435 107
Size: 974 354 2619
Fragments arranged by size:
2619 974 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1530 1663 2122 2331 1530
Size: 133 459 209 3146
Fragments arranged by size:
3146 459 209 133
TatI w'GTAC_w
Cuts at: 3462 3462
Size: 3947
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3464 3496 3464
Size: 32 3915
Tth111I GACn'n_nGTC
Cuts at: 1744 1744
Size: 3947
XbaI T'CTAG_A
Cuts at: 978 978
Size: 3947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1442 1442
Size: 3947
XmaI C'CCGG_G
Cuts at: 967 967
Size: 3947
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BpmI Bpu10I BsaXI BsgI BsiWI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI