Restriction map for
pF1KB6302
(Circular) (Six-base) MAPSORT of: pF1KB6302.seq Check: 5340 from: 1 to: 3944
pF1KB6302 3944 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 982 982
Size: 3944
Acc65I G'GTAC_C
Cuts at: 960 960
Size: 3944
AclI AA'CG_TT
Cuts at: 3784 3784
Size: 3944
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 1769 2201 2948 3216 315
Size: 1454 432 747 268 1043
Fragments arranged by size:
1454 1043 747 432 268
AflIII A'CryG_T
Cuts at: 1296 2400 3377 1296
Size: 1104 977 1863
Fragments arranged by size:
1863 1104 977
AgeI A'CCGG_T
Cuts at: 2332 2332
Size: 3944
AleI CACnn'nnGTG
Cuts at: 665 665
Size: 3944
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 536 536
Size: 3944
AlwNI CAG_nnn'CTG
Cuts at: 128 744 1249 2816 128
Size: 616 505 1567 1256
Fragments arranged by size:
1567 1256 616 505
ApaLI G'TGCA_C
Cuts at: 2714 2714
Size: 3944
ApoI r'AATT_y
Cuts at: 948 3297 3553 948
Size: 2349 256 1339
Fragments arranged by size:
2349 1339 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3944
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3944
AvaI C'yCGr_G
Cuts at: 964 964
Size: 3944
BamHI G'GATC_C
Cuts at: 969 969
Size: 3944
BanI G'GyrC_C
Cuts at: 960 1374 1622 1657 960
Size: 414 248 35 3247
Fragments arranged by size:
3247 414 248 35
BanII G_rGCy'C
Cuts at: 958 1988 958
Size: 1030 2914
BbeI G_GCGC'C
Cuts at: 1378 1626 1378
Size: 248 3696
BciVI GTATCCnnnnn_n'
Cuts at: 1842 2603 3944 1842
Size: 761 1341 1842
Fragments arranged by size:
1842 1341 761
BclI T'GATC_A
Cuts at: 1464 1464
Size: 3944
BglII A'GATC_T
Cuts at: 653 1459 3496 653
Size: 806 2037 1101
Fragments arranged by size:
2037 1101 806
BlpI GC'TnA_GC
Cuts at: 1056 1056
Size: 3944
Bme1580I G_kGCm'C
Cuts at: 1569 1662 2718 1569
Size: 93 1056 2795
Fragments arranged by size:
2795 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1156 1237 1572 3716 1156
Size: 81 335 2144 1384
Fragments arranged by size:
2144 1384 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2311 2311
Size: 3944
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 150
Size: 3944
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1120 1999 2491 2789 1120
Size: 879 492 298 2275
Fragments arranged by size:
2275 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3648 3648
Size: 3944
BsaAI yAC'GTr
Cuts at: 1297 1297
Size: 3944
BsaBI GATnn'nnATC
Cuts at: 6 1458 3495 6
Size: 1452 2037 455
Fragments arranged by size:
2037 1452 455
BsaHI Gr'CG_yC
Cuts at: 1375 1623 2325 3821 1375
Size: 248 702 1496 1498
Fragments arranged by size:
1498 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1133 1654 2332 2606 2753 3164 1133
Size: 521 678 274 147 411 1913
Fragments arranged by size:
1913 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 137 167 137
Size: 30 3914
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 598 916 598
Size: 318 3626
BseYI C'CCAG_C
Cuts at: 894 1369 2127 2704 3200 894
Size: 475 758 577 496 1638
Fragments arranged by size:
1638 758 577 496 475
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 542
Size: 3944
BsiEI CG_ry'CG
Cuts at: 104 1486 1532 2740 104
Size: 1382 46 1208 1308
Fragments arranged by size:
1382 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 295 958 1736 1926 2718 295
Size: 663 778 190 792 1521
Fragments arranged by size:
1521 792 778 663 190
Bsp1286I G_dGCh'C
Cuts at: 295 958 1569 1662 1736 1926 1988 2718 295
Size: 663 611 93 74 190 62 730 1521
Fragments arranged by size:
1521 730 663 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3164 3164
Size: 3944
BspHI T'CATG_A
Cuts at: 3120 3120
Size: 3944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 994 1208 1510 1891 994
Size: 214 302 381 3047
Fragments arranged by size:
3047 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2236 2290 3774 3939 50
Size: 2186 54 1484 165 55
Fragments arranged by size:
2186 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1856 1856
Size: 3944
BsrFI r'CCGG_y
Cuts at: 1942 2123 2332 1942
Size: 181 209 3554
Fragments arranged by size:
3554 209 181
BssSI C'ACGA_G
Cuts at: 2215 2573 2215
Size: 358 3586
BstAPI GCAn_nnn'nTGC
Cuts at: 1321 1321
Size: 3944
BstBI TT'CG_AA
Cuts at: 2305 2305
Size: 3944
BstXI CCAn_nnnn'nTGG
Cuts at: 364 765 364
Size: 401 3543
BstYI r'GATC_y
Cuts at: 1 653 798 969 1436 1459 1794 2040 3052
Size: 652 145 171 467 23 335 246 1012
Cuts at: 3052 3138 3150 3496 1
Size: 86 12 346 449
Fragments arranged by size:
1012 652 467 449 346 335 246 171
145 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1894 2075 2238 1894
Size: 181 163 3600
Fragments arranged by size:
3600 181 163
BtsI GCAGTG_nn'
Cuts at: 173 530 1187 173
Size: 357 657 2930
Fragments arranged by size:
2930 657 357
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 483 518 483
Size: 35 3909
DraI TTT'AAA
Cuts at: 943 943
Size: 3944
DrdI GACnn_nn'nnGTC
Cuts at: 1650 2508 1650
Size: 858 3086
EaeI y'GGCC_r
Cuts at: 1529 1703 2094 2121 3466 1529
Size: 174 391 27 1345 2007
Fragments arranged by size:
2007 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1529 1529
Size: 3944
EarI CTCTTCn'nnn_
Cuts at: 250 1967 2177 250
Size: 1717 210 2017
Fragments arranged by size:
2017 1717 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2152 2462 2608 3754 2152
Size: 310 146 1146 2342
Fragments arranged by size:
2342 1146 310 146
EcoICRI GAG'CTC
Cuts at: 956 956
Size: 3944
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 315 1769 2201 2948 3216 150
Size: 165 1454 432 747 268 878
Fragments arranged by size:
1454 878 747 432 268 165
EcoO109I rG'GnC_Cy
Cuts at: 1083 1083
Size: 3944
EcoRI G'AATT_C
Cuts at: 948 948
Size: 3944
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1963 1963
Size: 3944
FspI TGC'GCA
Cuts at: 1725 1725
Size: 3944
HaeII r_GCGC'y
Cuts at: 1378 1626 2648 3627 1378
Size: 248 1022 979 1695
Fragments arranged by size:
1695 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1786 1816 1818 1848 1786
Size: 30 2 30 3882
Fragments arranged by size:
3882 30 30 2
HincII GTy'rAC
Cuts at: 983 983
Size: 3944
HindIII A'AGCT_T
Cuts at: 282 282
Size: 3944
KasI G'GCGC_C
Cuts at: 1374 1622 1374
Size: 248 3696
KpnI G_GTAC'C
Cuts at: 964 964
Size: 3944
MluI A'CGCG_T
Cuts at: 3377 3377
Size: 3944
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2615 2799 2615
Size: 184 3760
MscI TGG'CCA
Cuts at: 1705 1705
Size: 3944
MslI CAynn'nnrTG
Cuts at: 665 1933 2060 665
Size: 1268 127 2549
Fragments arranged by size:
2549 1268 127
MspA1I CmG'CkG
Cuts at: 125 894 1055 1369 1729 2742 2987 125
Size: 769 161 314 360 1013 245 1082
Fragments arranged by size:
1082 1013 769 360 314 245 161
NaeI GCC'GGC
Cuts at: 2125 2125
Size: 3944
NarI GG'CG_CC
Cuts at: 1375 1623 1375
Size: 248 3696
NgoMIV G'CCGG_C
Cuts at: 2123 2123
Size: 3944
NspI r_CATG'y
Cuts at: 997 1497 2404 997
Size: 500 907 2537
Fragments arranged by size:
2537 907 500
PciI A'CATG_T
Cuts at: 2400 2400
Size: 3944
PflMI CCAn_nnn'nTGG
Cuts at: 744 744
Size: 3944
PmeI GTTT'AAAC
Cuts at: 943 943
Size: 3944
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3083 3115 3083
Size: 32 3912
PstI C_TGCA'G
Cuts at: 867 991 1676 867
Size: 124 685 3135
Fragments arranged by size:
3135 685 124
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3944
PvuII CAG'CTG
Cuts at: 125 894 1369 1729 125
Size: 769 475 360 2340
Fragments arranged by size:
2340 769 475 360
RsrII CG'GwC_CG
Cuts at: 2139 2139
Size: 3944
SacI G_AGCT'C
Cuts at: 958 958
Size: 3944
SalI G'TCGA_C
Cuts at: 981 981
Size: 3944
SapI GCTCTTCn'nnn_
Cuts at: 1967 2177 1967
Size: 210 3734
SbfI CC_TGCA'GG
Cuts at: 991 991
Size: 3944
ScaI AGT'ACT
Cuts at: 3461 3461
Size: 3944
SexAI A'CCwGG_T
Cuts at: 809 809
Size: 3944
SfcI C'TryA_G
Cuts at: 33 120 863 987 1672 2665 2856 33
Size: 87 743 124 685 993 191 1121
Fragments arranged by size:
1121 993 743 685 191 124 87
SfoI GGC'GCC
Cuts at: 1376 1624 1376
Size: 248 3696
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3944
SmaI CCC'GGG
Cuts at: 966 966
Size: 3944
SmlI C'TyrA_G
Cuts at: 1099 2014 2506 2768 1099
Size: 915 492 262 2275
Fragments arranged by size:
2275 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3944
SphI G_CATG'C
Cuts at: 997 1497 997
Size: 500 3444
StuI AGG'CCT
Cuts at: 374 374
Size: 3944
StyI C'CwwG_G
Cuts at: 1078 1432 1078
Size: 354 3590
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1527 1660 2119 2328 1527
Size: 133 459 209 3143
Fragments arranged by size:
3143 459 209 133
TatI w'GTAC_w
Cuts at: 3459 3459
Size: 3944
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3461 3493 3461
Size: 32 3912
Tth111I GACn'n_nGTC
Cuts at: 832 1741 832
Size: 909 3035
XbaI T'CTAG_A
Cuts at: 975 975
Size: 3944
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1439 1439
Size: 3944
XmaI C'CCGG_G
Cuts at: 964 964
Size: 3944
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I BtgI ClaI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI