Restriction map for
pF1KB6290
(Circular) (Six-base) MAPSORT of: pF1KB6290.seq Check: 597 from: 1 to: 5468
pF1KB6290 5468 bp
With 182 enzymes: SgfI *
September 25, 2008 15:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1837 1930 2042 2409 1837
Size: 93 112 367 4896
Fragments arranged by size:
4896 367 112 93
AccI GT'mk_AC
Cuts at: 2506 2506
Size: 5468
Acc65I G'GTAC_C
Cuts at: 2484 2484
Size: 5468
AclI AA'CG_TT
Cuts at: 5308 5308
Size: 5468
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1411 1709 2312 3293 3725 4472 4740 1411
Size: 298 603 981 432 747 268 2139
Fragments arranged by size:
2139 981 747 603 432 298 268
AfeI AGC'GCT
Cuts at: 2188 2188
Size: 5468
AflIII A'CryG_T
Cuts at: 2110 2820 3924 4901 2110
Size: 710 1104 977 2677
Fragments arranged by size:
2677 1104 977 710
AgeI A'CCGG_T
Cuts at: 3856 3856
Size: 5468
AhdI GACnn_n'nnGTC
Cuts at: 2208 2208
Size: 5468
AleI CACnn'nnGTG
Cuts at: 2150 2150
Size: 5468
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 324 692 1938 324
Size: 368 1246 3854
Fragments arranged by size:
3854 1246 368
AlwNI CAG_nnn'CTG
Cuts at: 535 1657 2773 4340 535
Size: 1122 1116 1567 1663
Fragments arranged by size:
1663 1567 1122 1116
ApaI G_GGCC'C
Cuts at: 358 690 1481 358
Size: 332 791 4345
Fragments arranged by size:
4345 791 332
ApaLI G'TGCA_C
Cuts at: 1738 4238 1738
Size: 2500 2968
ApoI r'AATT_y
Cuts at: 1301 2161 2472 4821 5077 1301
Size: 860 311 2349 256 1692
Fragments arranged by size:
2349 1692 860 311 256
AseI AT'TA_AT
Cuts at: 20 2441 20
Size: 2421 3047
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5468
AvaI C'yCGr_G
Cuts at: 357 1956 2488 357
Size: 1599 532 3337
Fragments arranged by size:
3337 1599 532
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 857 890 857
Size: 33 5435
BamHI G'GATC_C
Cuts at: 1962 2493 1962
Size: 531 4937
BanI G'GyrC_C
Cuts at: 205 415 2484 2898 3146 3181 205
Size: 210 2069 414 248 35 2492
Fragments arranged by size:
2492 2069 414 248 210 35
BanII G_rGCy'C
Cuts at: 358 690 1481 1797 1957 2482 3512 358
Size: 332 791 316 160 525 1030 2314
Fragments arranged by size:
2314 1030 791 525 332 316 160
BbeI G_GCGC'C
Cuts at: 2902 3150 2902
Size: 248 5220
BbsI GAAGACnn'nnnn_
Cuts at: 506 506
Size: 5468
BbvCI CC'TCA_GC
Cuts at: 1316 1626 1316
Size: 310 5158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2442 2476 2442
Size: 34 5434
BciVI GTATCCnnnnn_n'
Cuts at: 3366 4127 5468 3366
Size: 761 1341 3366
Fragments arranged by size:
3366 1341 761
BclI T'GATC_A
Cuts at: 1785 2988 1785
Size: 1203 4265
BglI GCCn_nnn'nGGC
Cuts at: 685 1345 2362 685
Size: 660 1017 3791
Fragments arranged by size:
3791 1017 660
BglII A'GATC_T
Cuts at: 2427 2983 5020 2427
Size: 556 2037 2875
Fragments arranged by size:
2875 2037 556
BlpI GC'TnA_GC
Cuts at: 1796 2580 1796
Size: 784 4684
Bme1580I G_kGCm'C
Cuts at: 358 690 1481 1742 2043 2275 3093 3186 4242
Size: 332 791 261 301 232 818 93 1056
Cuts at: 4242 358
Size: 1584
Fragments arranged by size:
1584 1056 818 791 332 301 261 232
93
BmrI ACTGGGnnnn_n'
Cuts at: 692 2680 2761 3096 5240 692
Size: 1988 81 335 2144 920
Fragments arranged by size:
2144 1988 920 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1008 3835 1008
Size: 2827 2641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 623 787 1256 1327 1396 1405 1678 275
Size: 348 164 469 71 69 9 273 4065
Fragments arranged by size:
4065 469 348 273 164 71 69 9
Bpu10I CC'TnA_GC
Cuts at: 1316 1557 1626 2183 1316
Size: 241 69 557 4601
Fragments arranged by size:
4601 557 241 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1145 1373 2644 3523 4015 4313 1145
Size: 228 1271 879 492 298 2300
Fragments arranged by size:
2300 1271 879 492 298 228
BsaI GGTCTCn'nnnn_
Cuts at: 1810 5172 1810
Size: 3362 2106
BsaAI yAC'GTr
Cuts at: 2821 2821
Size: 5468
BsaBI GATnn'nnATC
Cuts at: 6 2982 5019 6
Size: 2976 2037 455
Fragments arranged by size:
2976 2037 455
BsaHI Gr'CG_yC
Cuts at: 2899 3147 3849 5345 2899
Size: 248 702 1496 3022
Fragments arranged by size:
3022 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1460 2657 3178 3856 4130 4277 4688 1460
Size: 1197 521 678 274 147 411 2240
Fragments arranged by size:
2240 1197 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 924 954 1325 1355 2307 2337 924
Size: 30 371 30 952 30 4055
Fragments arranged by size:
4055 952 371 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 272 275 435 1668 2211 272
Size: 3 160 1233 543 3529
Fragments arranged by size:
3529 1233 543 160 3
BseYI C'CCAG_C
Cuts at: 275 943 2893 3651 4228 4724 275
Size: 668 1950 758 577 496 1019
Fragments arranged by size:
1950 1019 758 668 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 715
Size: 5468
BsiEI CG_ry'CG
Cuts at: 104 1896 3010 3056 4264 104
Size: 1792 1114 46 1208 1308
Fragments arranged by size:
1792 1308 1208 1114 46
BsiHKAI G_wGCw'C
Cuts at: 1742 1797 2482 3260 3450 4242 1742
Size: 55 685 778 190 792 2968
Fragments arranged by size:
2968 792 778 685 190 55
BsmI GAATG_Cn'
Cuts at: 1668 1668
Size: 5468
Bsp1286I G_dGCh'C
Cuts at: 358 690 1481 1742 1797 1957 2043 2275 2482
Size: 332 791 261 55 160 86 232 207
Cuts at: 2482 3093 3186 3260 3450 3512 4242 358
Size: 611 93 74 190 62 730 1584
Fragments arranged by size:
1584 791 730 611 332 261 232 207
190 160 93 86 74 62 55
BspEI T'CCGG_A
Cuts at: 1460 4688 1460
Size: 3228 2240
BspHI T'CATG_A
Cuts at: 2192 4644 2192
Size: 2452 3016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1837 1930 2042 2287 2409 2518 2732 3034 3415
Size: 93 112 245 122 109 214 302 381
Cuts at: 3415 1837
Size: 3890
Fragments arranged by size:
3890 381 302 245 214 122 112 109
93
BsrBI CCG'CTC
Cuts at: 50 562 3760 3814 5298 5463 50
Size: 512 3198 54 1484 165 55
Fragments arranged by size:
3198 1484 512 165 55 54
BsrDI GCAATG_nn'
Cuts at: 195 507 779 1243 1619 2381 2460 3380 195
Size: 312 272 464 376 762 79 920 2283
Fragments arranged by size:
2283 920 762 464 376 312 272 79
BsrFI r'CCGG_y
Cuts at: 1891 3466 3647 3856 1891
Size: 1575 181 209 3503
Fragments arranged by size:
3503 1575 209 181
BssSI C'ACGA_G
Cuts at: 3739 4097 3739
Size: 358 5110
BstAPI GCAn_nnn'nTGC
Cuts at: 666 2845 666
Size: 2179 3289
BstBI TT'CG_AA
Cuts at: 3829 3829
Size: 5468
BstXI CCAn_nnnn'nTGG
Cuts at: 988 1113 1234 1816 2005 2349 988
Size: 125 121 582 189 344 4107
Fragments arranged by size:
4107 582 344 189 125 121
BstYI r'GATC_y
Cuts at: 1 1962 2427 2493 2960 2983 3318 3564 4576
Size: 1961 465 66 467 23 335 246 1012
Cuts at: 4576 4662 4674 5020 1
Size: 86 12 346 449
Fragments arranged by size:
1961 1012 467 465 449 346 335 246
86 66 23 12
Bsu36I CC'TnA_GG
Cuts at: 1008 1008
Size: 5468
BtgI C'CryG_G
Cuts at: 128 1809 2357 128
Size: 1681 548 3239
Fragments arranged by size:
3239 1681 548
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3418 3599 3762 3418
Size: 181 163 5124
Fragments arranged by size:
5124 181 163
BtsI GCAGTG_nn'
Cuts at: 513 2315 2316 2711 513
Size: 1802 1 395 3270
Fragments arranged by size:
3270 1802 395 1
DraI TTT'AAA
Cuts at: 2467 2467
Size: 5468
DraIII CAC_nnn'GTG
Cuts at: 415 415
Size: 5468
DrdI GACnn_nn'nnGTC
Cuts at: 3174 4032 3174
Size: 858 4610
EaeI y'GGCC_r
Cuts at: 3053 3227 3618 3645 4990 3053
Size: 174 391 27 1345 3531
Fragments arranged by size:
3531 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3053 3053
Size: 5468
EarI CTCTTCn'nnn_
Cuts at: 444 3491 3701 444
Size: 3047 210 2211
Fragments arranged by size:
3047 2211 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3676 3986 4132 5278 3676
Size: 310 146 1146 3866
Fragments arranged by size:
3866 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1795 2480 1795
Size: 685 4783
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 623 787 1256 1327 1396 1405 1411 1678
Size: 348 164 469 71 69 9 6 267
Cuts at: 1678 1709 2312 3293 3725 4472 4740 275
Size: 31 603 981 432 747 268 1003
Fragments arranged by size:
1003 981 747 603 469 432 348 268
267 164 71 69 31 9 6
EcoO109I rG'GnC_Cy
Cuts at: 354 686 977 1338 1347 1477 1901 2607 354
Size: 332 291 361 9 130 424 706 3215
Fragments arranged by size:
3215 706 424 361 332 291 130 9
EcoRI G'AATT_C
Cuts at: 2472 2472
Size: 5468
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3487 3487
Size: 5468
FspI TGC'GCA
Cuts at: 3249 3249
Size: 5468
HaeII r_GCGC'y
Cuts at: 2190 2902 3150 4172 5151 2190
Size: 712 248 1022 979 2507
Fragments arranged by size:
2507 1022 979 712 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 761 793 1143 1175 1325 1357 2194 2226 2419
Size: 32 350 32 150 32 837 32 193
Cuts at: 2419 2451 3310 3340 3342 3372 761
Size: 32 859 30 2 30 2857
Fragments arranged by size:
2857 859 837 350 193 150 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 915 2507 915
Size: 1592 3876
KasI G'GCGC_C
Cuts at: 2898 3146 2898
Size: 248 5220
KpnI G_GTAC'C
Cuts at: 2488 2488
Size: 5468
MluI A'CGCG_T
Cuts at: 4901 4901
Size: 5468
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1876 4139 4323 1876
Size: 2263 184 3021
Fragments arranged by size:
3021 2263 184
MscI TGG'CCA
Cuts at: 3229 3229
Size: 5468
MslI CAynn'nnrTG
Cuts at: 2076 2150 2347 2419 3457 3584 2076
Size: 74 197 72 1038 127 3960
Fragments arranged by size:
3960 1038 197 127 74 72
MspA1I CmG'CkG
Cuts at: 145 666 1512 1630 2579 2893 3253 4266 4511
Size: 521 846 118 949 314 360 1013 245
Cuts at: 4511 145
Size: 1102
Fragments arranged by size:
1102 1013 949 846 521 360 314 245
118
NaeI GCC'GGC
Cuts at: 3649 3649
Size: 5468
NarI GG'CG_CC
Cuts at: 2899 3147 2899
Size: 248 5220
NcoI C'CATG_G
Cuts at: 128 1809 2357 128
Size: 1681 548 3239
Fragments arranged by size:
3239 1681 548
NgoMIV G'CCGG_C
Cuts at: 3647 3647
Size: 5468
NspI r_CATG'y
Cuts at: 1919 2521 3021 3928 1919
Size: 602 500 907 3459
Fragments arranged by size:
3459 907 602 500
PasI CC'CwG_GG
Cuts at: 351 351
Size: 5468
PciI A'CATG_T
Cuts at: 3924 3924
Size: 5468
PflMI CCAn_nnn'nTGG
Cuts at: 425 828 1444 425
Size: 403 616 4449
Fragments arranged by size:
4449 616 403
PmeI GTTT'AAAC
Cuts at: 2467 2467
Size: 5468
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4607 4639 4607
Size: 32 5436
PpuMI rG'GwC_Cy
Cuts at: 977 1901 977
Size: 924 4544
PspOMI G'GGCC_C
Cuts at: 354 686 1477 354
Size: 332 791 4345
Fragments arranged by size:
4345 791 332
PstI C_TGCA'G
Cuts at: 671 930 1517 2435 2515 3200 671
Size: 259 587 918 80 685 2939
Fragments arranged by size:
2939 918 685 587 259 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5468
PvuII CAG'CTG
Cuts at: 145 666 1512 1630 2893 3253 145
Size: 521 846 118 1263 360 2360
Fragments arranged by size:
2360 1263 846 521 360 118
RsrII CG'GwC_CG
Cuts at: 3663 3663
Size: 5468
SacI G_AGCT'C
Cuts at: 1797 2482 1797
Size: 685 4783
SalI G'TCGA_C
Cuts at: 2505 2505
Size: 5468
SanDI GG'GwC_CC
Cuts at: 977 977
Size: 5468
SapI GCTCTTCn'nnn_
Cuts at: 3491 3701 3491
Size: 210 5258
SbfI CC_TGCA'GG
Cuts at: 2515 2515
Size: 5468
ScaI AGT'ACT
Cuts at: 4985 4985
Size: 5468
SexAI A'CCwGG_T
Cuts at: 729 2083 729
Size: 1354 4114
SfcI C'TryA_G
Cuts at: 33 667 926 1192 1507 1513 1766 2125 2431
Size: 634 259 266 315 6 253 359 306
Cuts at: 2431 2511 3196 4189 4380 33
Size: 80 685 993 191 1121
Fragments arranged by size:
1121 993 685 634 359 315 306 266
259 253 191 80 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 1345 1345
Size: 5468
SfoI GGC'GCC
Cuts at: 2900 3148 2900
Size: 248 5220
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5468
SmaI CCC'GGG
Cuts at: 2490 2490
Size: 5468
SmlI C'TyrA_G
Cuts at: 1160 1388 2623 3538 4030 4292 1160
Size: 228 1235 915 492 262 2336
Fragments arranged by size:
2336 1235 915 492 262 228
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5468
SphI G_CATG'C
Cuts at: 1919 2521 3021 1919
Size: 602 500 4366
Fragments arranged by size:
4366 602 500
SspI AAT'ATT
Cuts at: 618 618
Size: 5468
StuI AGG'CCT
Cuts at: 827 1691 1754 827
Size: 864 63 4541
Fragments arranged by size:
4541 864 63
StyI C'CwwG_G
Cuts at: 128 822 1580 1809 1998 2357 2602 2956 128
Size: 694 758 229 189 359 245 354 2640
Fragments arranged by size:
2640 758 694 359 354 245 229 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 342 1632 1757 3051 3184 3643 3852 342
Size: 1290 125 1294 133 459 209 1958
Fragments arranged by size:
1958 1294 1290 459 209 133 125
TatI w'GTAC_w
Cuts at: 840 4983 840
Size: 4143 1325
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1721 1753 2307 2339 4985 5017 1721
Size: 32 554 32 2646 32 2172
Fragments arranged by size:
2646 2172 554 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3265 3265
Size: 5468
XbaI T'CTAG_A
Cuts at: 2499 2499
Size: 5468
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 652 876 1110 1183 1816 2963 652
Size: 224 234 73 633 1147 3157
Fragments arranged by size:
3157 1147 633 234 224 73
XmaI C'CCGG_G
Cuts at: 2488 2488
Size: 5468
XmnI GAAnn'nnTTC
Cuts at: 2104 2104
Size: 5468
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AloI AscI AvrII BfrBI BmgBI BmtI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SgrAI SnaBI SrfI SwaI
XhoI ZraI