Restriction map for
pF1KB6271
(Circular) (Six-base) MAPSORT of: pF1KB6271.seq Check: 9671 from: 1 to: 5123
pF1KB6271 5123 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 999 1787 1854 999
Size: 788 67 4268
Fragments arranged by size:
4268 788 67
AccI GT'mk_AC
Cuts at: 475 2161 475
Size: 1686 3437
Acc65I G'GTAC_C
Cuts at: 2139 2139
Size: 5123
AclI AA'CG_TT
Cuts at: 4963 4963
Size: 5123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 469 505 849 1357 1774 1852 2948 3380
Size: 90 36 344 508 417 78 1096 432
Cuts at: 3380 4127 4395 379
Size: 747 268 1107
Fragments arranged by size:
1107 1096 747 508 432 417 344 268
90 78 36
AfeI AGC'GCT
Cuts at: 915 1444 915
Size: 529 4594
AflIII A'CryG_T
Cuts at: 2475 3579 4556 2475
Size: 1104 977 3042
Fragments arranged by size:
3042 1104 977
AgeI A'CCGG_T
Cuts at: 1625 3511 1625
Size: 1886 3237
AlwNI CAG_nnn'CTG
Cuts at: 357 1302 1437 2428 3995 357
Size: 945 135 991 1567 1485
Fragments arranged by size:
1567 1485 991 945 135
ApaI G_GGCC'C
Cuts at: 222 1601 1646 1933 222
Size: 1379 45 287 3412
Fragments arranged by size:
3412 1379 287 45
ApaLI G'TGCA_C
Cuts at: 502 1075 3893 502
Size: 573 2818 1732
Fragments arranged by size:
2818 1732 573
ApoI r'AATT_y
Cuts at: 2127 4476 4732 2127
Size: 2349 256 2518
Fragments arranged by size:
2518 2349 256
AscI GG'CGCG_CC
Cuts at: 793 793
Size: 5123
AseI AT'TA_AT
Cuts at: 20 20
Size: 5123
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5123
AvaI C'yCGr_G
Cuts at: 673 1277 1658 2143 673
Size: 604 381 485 3653
Fragments arranged by size:
3653 604 485 381
BamHI G'GATC_C
Cuts at: 2148 2148
Size: 5123
BanI G'GyrC_C
Cuts at: 139 520 769 820 1852 2139 2553 2801 2836
Size: 381 249 51 1032 287 414 248 35
Cuts at: 2836 139
Size: 2426
Fragments arranged by size:
2426 1032 414 381 287 249 248 51
35
BanII G_rGCy'C
Cuts at: 157 222 380 680 904 1229 1469 1601 1646
Size: 65 158 300 224 325 240 132 45
Cuts at: 1646 1933 2137 3167 157
Size: 287 204 1030 2113
Fragments arranged by size:
2113 1030 325 300 287 240 224 204
158 132 65 45
BbeI G_GCGC'C
Cuts at: 143 524 1856 2557 2805 143
Size: 381 1332 701 248 2461
Fragments arranged by size:
2461 1332 701 381 248
BbsI GAAGACnn'nnnn_
Cuts at: 1239 1239
Size: 5123
BciVI GTATCCnnnnn_n'
Cuts at: 3021 3782 5123 3021
Size: 761 1341 3021
Fragments arranged by size:
3021 1341 761
BclI T'GATC_A
Cuts at: 2643 2643
Size: 5123
BglI GCCn_nnn'nGGC
Cuts at: 859 1000 1054 1744 859
Size: 141 54 690 4238
Fragments arranged by size:
4238 690 141 54
BglII A'GATC_T
Cuts at: 2638 4675 2638
Size: 2037 3086
BlpI GC'TnA_GC
Cuts at: 328 379 2235 328
Size: 51 1856 3216
Fragments arranged by size:
3216 1856 51
Bme1580I G_kGCm'C
Cuts at: 222 506 732 774 825 1079 1601 1646 1933
Size: 284 226 42 51 254 522 45 287
Cuts at: 1933 2070 2748 2841 3897 222
Size: 137 678 93 1056 1448
Fragments arranged by size:
1448 1056 678 522 287 284 254 226
137 93 51 45 42
BmrI ACTGGGnnnn_n'
Cuts at: 716 2065 2335 2416 2751 4895 716
Size: 1349 270 81 335 2144 944
Fragments arranged by size:
2144 1349 944 335 270 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 513 903 999 3490 513
Size: 390 96 2491 2146
Fragments arranged by size:
2491 2146 390 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 343 391 571 622 955 1324 2037 2066
Size: 207 48 180 51 333 369 713 29
Cuts at: 2066 136
Size: 3193
Fragments arranged by size:
3193 713 369 333 207 180 51 48
29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2299 3178 3670 3968 2299
Size: 879 492 298 3454
Fragments arranged by size:
3454 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 448 4827 448
Size: 4379 744
BsaAI yAC'GTr
Cuts at: 1113 1182 1988 2476 1113
Size: 69 806 488 3760
Fragments arranged by size:
3760 806 488 69
BsaBI GATnn'nnATC
Cuts at: 6 2637 4674 6
Size: 2631 2037 455
Fragments arranged by size:
2631 2037 455
BsaHI Gr'CG_yC
Cuts at: 140 521 1853 2554 2802 3504 5000 140
Size: 381 1332 701 248 702 1496 263
Fragments arranged by size:
1496 1332 702 701 381 263 248
BsaWI w'CCGG_w
Cuts at: 1079 1625 2312 2833 3511 3785 3932 4343 1079
Size: 546 687 521 678 274 147 411 1859
Fragments arranged by size:
1859 687 678 546 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 325 355 508 538 1147 1177 325
Size: 30 153 30 609 30 4271
Fragments arranged by size:
4271 609 153 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 163 379 388 1257 1321 1375 1676 1999
Size: 30 216 9 869 64 54 301 323
Cuts at: 1999 133
Size: 3257
Fragments arranged by size:
3257 869 323 301 216 64 54 30
9
BseYI C'CCAG_C
Cuts at: 580 719 856 1022 1091 1209 1593 1882 1897
Size: 139 137 166 69 118 384 289 15
Cuts at: 1897 1926 2030 2057 2548 3306 3883 4379 580
Size: 29 104 27 491 758 577 496 1324
Fragments arranged by size:
1324 758 577 496 491 384 289 166
139 137 118 104 69 29 27 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 795 1360 795
Size: 565 4558
BsiEI CG_ry'CG
Cuts at: 104 317 1217 1473 2665 2711 3919 104
Size: 213 900 256 1192 46 1208 1308
Fragments arranged by size:
1308 1208 1192 900 256 213 46
BsiHKAI G_wGCw'C
Cuts at: 380 506 1079 1229 1469 2137 2915 3105 3897
Size: 126 573 150 240 668 778 190 792
Cuts at: 3897 380
Size: 1606
Fragments arranged by size:
1606 792 778 668 573 240 190 150
126
BsmBI CGTCTCn'nnnn_
Cuts at: 304 304
Size: 5123
Bsp1286I G_dGCh'C
Cuts at: 157 222 380 506 680 732 774 825 904
Size: 65 158 126 174 52 42 51 79
Cuts at: 904 1079 1229 1469 1601 1646 1933 2070 2137
Size: 175 150 240 132 45 287 137 67
Cuts at: 2137 2748 2841 2915 3105 3167 3897 157
Size: 611 93 74 190 62 730 1383
Fragments arranged by size:
1383 730 611 287 240 190 175 174
158 150 137 132 126 93 79 74
67 65 62 52 51 45 42
BspEI T'CCGG_A
Cuts at: 4343 4343
Size: 5123
BspHI T'CATG_A
Cuts at: 944 4299 944
Size: 3355 1768
BspMI ACCTGCnnnn'nnnn_
Cuts at: 356 999 1233 1418 1787 1854 2173 2387 2689
Size: 643 234 185 369 67 319 214 302
Cuts at: 2689 3070 356
Size: 381 2409
Fragments arranged by size:
2409 643 381 369 319 302 234 214
185 67
BsrBI CCG'CTC
Cuts at: 50 806 1163 3415 3469 4953 5118 50
Size: 756 357 2252 54 1484 165 55
Fragments arranged by size:
2252 1484 756 357 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3035 3035
Size: 5123
BsrFI r'CCGG_y
Cuts at: 142 401 1625 1913 2115 3121 3302 3511 142
Size: 259 1224 288 202 1006 181 209 1754
Fragments arranged by size:
1754 1224 1006 288 259 209 202 181
BssHII G'CGCG_C
Cuts at: 646 793 646
Size: 147 4976
BssSI C'ACGA_G
Cuts at: 1237 3394 3752 1237
Size: 2157 358 2608
Fragments arranged by size:
2608 2157 358
BstAPI GCAn_nnn'nTGC
Cuts at: 570 2500 570
Size: 1930 3193
BstBI TT'CG_AA
Cuts at: 3484 3484
Size: 5123
BstXI CCAn_nnnn'nTGG
Cuts at: 114 465 1799 114
Size: 351 1334 3438
Fragments arranged by size:
3438 1334 351
BstYI r'GATC_y
Cuts at: 1 188 2148 2615 2638 2973 3219 4231 4317
Size: 187 1960 467 23 335 246 1012 86
Cuts at: 4317 4329 4675 1
Size: 12 346 449
Fragments arranged by size:
1960 1012 467 449 346 335 246 187
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 874 874
Size: 5123
BtgI C'CryG_G
Cuts at: 157 998 1085 1187 1739 157
Size: 841 87 102 552 3541
Fragments arranged by size:
3541 841 552 102 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1695 3073 3254 3417 1695
Size: 1378 181 163 3401
Fragments arranged by size:
3401 1378 181 163
BtsI GCAGTG_nn'
Cuts at: 2366 2366
Size: 5123
DraI TTT'AAA
Cuts at: 2122 2122
Size: 5123
DrdI GACnn_nn'nnGTC
Cuts at: 1702 2829 3687 1702
Size: 1127 858 3138
Fragments arranged by size:
3138 1127 858
EaeI y'GGCC_r
Cuts at: 198 399 1451 2113 2708 2882 3273 3300 4645
Size: 201 1052 662 595 174 391 27 1345
Cuts at: 4645 198
Size: 676
Fragments arranged by size:
1345 1052 676 662 595 391 201 174
27
EagI C'GGCC_G
Cuts at: 2708 2708
Size: 5123
EarI CTCTTCn'nnn_
Cuts at: 685 763 3146 3356 685
Size: 78 2383 210 2452
Fragments arranged by size:
2452 2383 210 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2075 3331 3641 3787 4933 2075
Size: 1256 310 146 1146 2265
Fragments arranged by size:
2265 1256 1146 310 146
EcoICRI GAG'CTC
Cuts at: 378 1227 1467 2135 378
Size: 849 240 668 3366
Fragments arranged by size:
3366 849 668 240
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 343 379 391 469 505 571 622 849
Size: 207 36 12 78 36 66 51 227
Cuts at: 849 955 1324 1357 1774 1852 2037 2066 2948
Size: 106 369 33 417 78 185 29 882
Cuts at: 2948 3380 4127 4395 136
Size: 432 747 268 864
Fragments arranged by size:
882 864 747 432 417 369 268 227
207 185 106 78 78 66 51 36
36 33 29 12
EcoNI CCTnn'n_nnAGG
Cuts at: 562 846 1036 1354 1399 1561 1756 562
Size: 284 190 318 45 162 195 3929
Fragments arranged by size:
3929 318 284 195 190 162 45
EcoO109I rG'GnC_Cy
Cuts at: 218 555 711 860 978 1282 1503 1573 1597
Size: 337 156 149 118 304 221 70 24
Cuts at: 1597 1598 1642 1930 2262 218
Size: 1 44 288 332 3079
Fragments arranged by size:
3079 337 332 304 288 221 156 149
118 70 44 24 1
EcoRI G'AATT_C
Cuts at: 2127 2127
Size: 5123
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3142 3142
Size: 5123
FspI TGC'GCA
Cuts at: 930 2904 930
Size: 1974 3149
HaeII r_GCGC'y
Cuts at: 143 269 524 917 1369 1446 1856 2557 2805
Size: 126 255 393 452 77 410 701 248
Cuts at: 2805 3827 4806 143
Size: 1022 979 460
Fragments arranged by size:
1022 979 701 460 452 410 393 255
248 126 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 299 331 508 540 2965 2995 2997 3027 299
Size: 32 177 32 2425 30 2 30 2395
Fragments arranged by size:
2425 2395 177 32 32 30 30 2
HincII GTy'rAC
Cuts at: 339 2162 339
Size: 1823 3300
KasI G'GCGC_C
Cuts at: 139 520 1852 2553 2801 139
Size: 381 1332 701 248 2461
Fragments arranged by size:
2461 1332 701 381 248
KpnI G_GTAC'C
Cuts at: 2143 2143
Size: 5123
MluI A'CGCG_T
Cuts at: 4556 4556
Size: 5123
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 368 3794 3978 368
Size: 3426 184 1513
Fragments arranged by size:
3426 1513 184
MscI TGG'CCA
Cuts at: 1453 2884 1453
Size: 1431 3692
MslI CAynn'nnrTG
Cuts at: 1797 1986 3112 3239 1797
Size: 189 1126 127 3681
Fragments arranged by size:
3681 1126 189 127
MspA1I CmG'CkG
Cuts at: 159 204 354 383 483 630 856 1026 1741
Size: 45 150 29 100 147 226 170 715
Cuts at: 1741 2234 2548 2908 3921 4166 159
Size: 493 314 360 1013 245 1116
Fragments arranged by size:
1116 1013 715 493 360 314 245 226
170 150 147 100 45 29
NaeI GCC'GGC
Cuts at: 144 403 1915 3304 144
Size: 259 1512 1389 1963
Fragments arranged by size:
1963 1512 1389 259
NarI GG'CG_CC
Cuts at: 140 521 1853 2554 2802 140
Size: 381 1332 701 248 2461
Fragments arranged by size:
2461 1332 701 381 248
NcoI C'CATG_G
Cuts at: 998 1085 1187 998
Size: 87 102 4934
Fragments arranged by size:
4934 102 87
NgoMIV G'CCGG_C
Cuts at: 142 401 1913 3302 142
Size: 259 1512 1389 1963
Fragments arranged by size:
1963 1512 1389 259
NspI r_CATG'y
Cuts at: 1144 2176 2676 3583 1144
Size: 1032 500 907 2684
Fragments arranged by size:
2684 1032 907 500
PasI CC'CwG_GG
Cuts at: 441 1563 1934 2107 441
Size: 1122 371 173 3457
Fragments arranged by size:
3457 1122 371 173
PciI A'CATG_T
Cuts at: 3579 3579
Size: 5123
PflMI CCAn_nnn'nTGG
Cuts at: 1593 1593
Size: 5123
PfoI T'CCnGG_A
Cuts at: 113 638 1123 113
Size: 525 485 4113
Fragments arranged by size:
4113 525 485
PmeI GTTT'AAAC
Cuts at: 2122 2122
Size: 5123
PmlI CAC'GTG
Cuts at: 1988 1988
Size: 5123
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 4262
Size: 32 5091
PpuMI rG'GwC_Cy
Cuts at: 711 711
Size: 5123
PspOMI G'GGCC_C
Cuts at: 218 1597 1642 1929 218
Size: 1379 45 287 3412
Fragments arranged by size:
3412 1379 287 45
PstI C_TGCA'G
Cuts at: 167 209 254 353 1256 1442 1865 2170 2855
Size: 42 45 99 903 186 423 305 685
Cuts at: 2855 167
Size: 2435
Fragments arranged by size:
2435 903 685 423 305 186 99 45
42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5123
PvuII CAG'CTG
Cuts at: 204 354 630 1026 2548 2908 204
Size: 150 276 396 1522 360 2419
Fragments arranged by size:
2419 1522 396 360 276 150
RsrII CG'GwC_CG
Cuts at: 3318 3318
Size: 5123
SacI G_AGCT'C
Cuts at: 380 1229 1469 2137 380
Size: 849 240 668 3366
Fragments arranged by size:
3366 849 668 240
SacII CC_GC'GG
Cuts at: 160 1742 160
Size: 1582 3541
SalI G'TCGA_C
Cuts at: 2160 2160
Size: 5123
SanDI GG'GwC_CC
Cuts at: 711 711
Size: 5123
SapI GCTCTTCn'nnn_
Cuts at: 685 3146 3356 685
Size: 2461 210 2452
Fragments arranged by size:
2461 2452 210
SbfI CC_TGCA'GG
Cuts at: 1865 2170 1865
Size: 305 4818
ScaI AGT'ACT
Cuts at: 1156 4640 1156
Size: 3484 1639
SfcI C'TryA_G
Cuts at: 33 163 205 250 279 349 1252 1438 1861
Size: 130 42 45 29 70 903 186 423
Cuts at: 1861 2166 2851 3844 4035 33
Size: 305 685 993 191 1121
Fragments arranged by size:
1121 993 903 685 423 305 191 186
130 70 45 42 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 859 859
Size: 5123
SfoI GGC'GCC
Cuts at: 141 522 1854 2555 2803 141
Size: 381 1332 701 248 2461
Fragments arranged by size:
2461 1332 701 381 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5123
SgrAI Cr'CCGG_yG
Cuts at: 142 142
Size: 5123
SmaI CCC'GGG
Cuts at: 2145 2145
Size: 5123
SmlI C'TyrA_G
Cuts at: 2278 3193 3685 3947 2278
Size: 915 492 262 3454
Fragments arranged by size:
3454 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5123
SphI G_CATG'C
Cuts at: 2176 2676 2176
Size: 500 4623
StuI AGG'CCT
Cuts at: 841 841
Size: 5123
StyI C'CwwG_G
Cuts at: 458 998 1085 1187 2257 2611 458
Size: 540 87 102 1070 354 2970
Fragments arranged by size:
2970 1070 540 354 102 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1231 2120 2706 2839 3298 3507 1231
Size: 889 586 133 459 209 2847
Fragments arranged by size:
2847 889 586 459 209 133
TatI w'GTAC_w
Cuts at: 1064 1154 4638 1064
Size: 90 3484 1549
Fragments arranged by size:
3484 1549 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4640 4672 4640
Size: 32 5091
Tth111I GACn'n_nGTC
Cuts at: 2920 2920
Size: 5123
XbaI T'CTAG_A
Cuts at: 1888 2154 1888
Size: 266 4857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 438 784 853 1799 1863 1960 2618 438
Size: 346 69 946 64 97 658 2943
Fragments arranged by size:
2943 946 658 346 97 69 64
XmaI C'CCGG_G
Cuts at: 2143 2143
Size: 5123
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI
BsrGI BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PshAI PsiI PspXI PsrI SexAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI