Restriction map for
pF1KB6259
(Circular) (Six-base) MAPSORT of: pF1KB6259.seq Check: 819 from: 1 to: 5405
pF1KB6259 5405 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1000 1000
Size: 5405
AccI GT'mk_AC
Cuts at: 401 2443 401
Size: 2042 3363
Acc65I G'GTAC_C
Cuts at: 2421 2421
Size: 5405
AclI AA'CG_TT
Cuts at: 5245 5245
Size: 5405
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2078 3230 3662 4409 4677 2078
Size: 1152 432 747 268 2806
Fragments arranged by size:
2806 1152 747 432 268
AfeI AGC'GCT
Cuts at: 949 949
Size: 5405
AflIII A'CryG_T
Cuts at: 2757 3861 4838 2757
Size: 1104 977 3324
Fragments arranged by size:
3324 1104 977
AgeI A'CCGG_T
Cuts at: 2192 3793 2192
Size: 1601 3804
AlwNI CAG_nnn'CTG
Cuts at: 2710 4277 2710
Size: 1567 3838
ApaLI G'TGCA_C
Cuts at: 4175 4175
Size: 5405
ApoI r'AATT_y
Cuts at: 787 985 1715 1805 2125 2200 2395 2409 4758
Size: 198 730 90 320 75 195 14 2349
Cuts at: 4758 5014 787
Size: 256 1178
Fragments arranged by size:
2349 1178 730 320 256 198 195 90
75 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 5405
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5405
AvaI C'yCGr_G
Cuts at: 1558 2425 1558
Size: 867 4538
BamHI G'GATC_C
Cuts at: 2430 2430
Size: 5405
BanI G'GyrC_C
Cuts at: 1357 1404 2379 2421 2835 3083 3118 1357
Size: 47 975 42 414 248 35 3644
Fragments arranged by size:
3644 975 414 248 47 42 35
BanII G_rGCy'C
Cuts at: 2419 3449 2419
Size: 1030 4375
BbeI G_GCGC'C
Cuts at: 1408 2839 3087 1408
Size: 1431 248 3726
Fragments arranged by size:
3726 1431 248
BbsI GAAGACnn'nnnn_
Cuts at: 201 880 201
Size: 679 4726
BciVI GTATCCnnnnn_n'
Cuts at: 933 1026 3303 4064 5405 933
Size: 93 2277 761 1341 933
Fragments arranged by size:
2277 1341 933 761 93
BclI T'GATC_A
Cuts at: 2925 2925
Size: 5405
BfrBI ATG'CAT
Cuts at: 430 430
Size: 5405
BglII A'GATC_T
Cuts at: 2920 4957 2920
Size: 2037 3368
BlpI GC'TnA_GC
Cuts at: 2517 2517
Size: 5405
Bme1580I G_kGCm'C
Cuts at: 974 3030 3123 4179 974
Size: 2056 93 1056 2200
Fragments arranged by size:
2200 2056 1056 93
BmgBI CAC'GTC
Cuts at: 1104 1104
Size: 5405
BmrI ACTGGGnnnn_n'
Cuts at: 406 1255 2300 2617 2698 3033 5177 406
Size: 849 1045 317 81 335 2144 634
Fragments arranged by size:
2144 1045 849 634 335 317 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3772 3772
Size: 5405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1284 1284
Size: 5405
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2581 3460 3952 4250 2581
Size: 879 492 298 3736
Fragments arranged by size:
3736 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5109 5109
Size: 5405
BsaAI yAC'GTr
Cuts at: 2362 2758 2362
Size: 396 5009
BsaBI GATnn'nnATC
Cuts at: 6 1220 1728 2919 4956 6
Size: 1214 508 1191 2037 455
Fragments arranged by size:
2037 1214 1191 508 455
BsaHI Gr'CG_yC
Cuts at: 1405 2836 3084 3786 5282 1405
Size: 1431 248 702 1496 1528
Fragments arranged by size:
1528 1496 1431 702 248
BsaWI w'CCGG_w
Cuts at: 2192 2594 3115 3793 4067 4214 4625 2192
Size: 402 521 678 274 147 411 2972
Fragments arranged by size:
2972 678 521 411 402 274 147
BseYI C'CCAG_C
Cuts at: 441 1282 1345 2830 3588 4165 4661 441
Size: 841 63 1485 758 577 496 1185
Fragments arranged by size:
1485 1185 841 758 577 496 63
BsiEI CG_ry'CG
Cuts at: 104 2947 2993 4201 104
Size: 2843 46 1208 1308
Fragments arranged by size:
2843 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1324 2419 3197 3387 4179 1324
Size: 1095 778 190 792 2550
Fragments arranged by size:
2550 1095 792 778 190
BsmI GAATG_Cn'
Cuts at: 1463 1463
Size: 5405
Bsp1286I G_dGCh'C
Cuts at: 974 1324 2419 3030 3123 3197 3387 3449 4179
Size: 350 1095 611 93 74 190 62 730
Cuts at: 4179 974
Size: 2200
Fragments arranged by size:
2200 1095 730 611 350 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4625 4625
Size: 5405
BspHI T'CATG_A
Cuts at: 1526 4581 1526
Size: 3055 2350
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1000 1988 2455 2669 2971 3352 1000
Size: 988 467 214 302 381 3053
Fragments arranged by size:
3053 988 467 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3697 3751 5235 5400 50
Size: 3647 54 1484 165 55
Fragments arranged by size:
3647 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 570 1154 3317 570
Size: 584 2163 2658
Fragments arranged by size:
2658 2163 584
BsrFI r'CCGG_y
Cuts at: 308 2192 3403 3584 3793 308
Size: 1884 1211 181 209 1920
Fragments arranged by size:
1920 1884 1211 209 181
BssSI C'ACGA_G
Cuts at: 3676 4034 3676
Size: 358 5047
BstAPI GCAn_nnn'nTGC
Cuts at: 1153 1863 2782 1153
Size: 710 919 3776
Fragments arranged by size:
3776 919 710
BstBI TT'CG_AA
Cuts at: 1803 3766 1803
Size: 1963 3442
BstXI CCAn_nnnn'nTGG
Cuts at: 1551 1551
Size: 5405
BstYI r'GATC_y
Cuts at: 1 2430 2897 2920 3255 3501 4513 4599 4611
Size: 2429 467 23 335 246 1012 86 12
Cuts at: 4611 4957 1
Size: 346 449
Fragments arranged by size:
2429 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 485 485
Size: 5405
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1128 3355 3536 3699 1128
Size: 2227 181 163 2834
Fragments arranged by size:
2834 2227 181 163
BtsI GCAGTG_nn'
Cuts at: 1169 2078 2648 1169
Size: 909 570 3926
Fragments arranged by size:
3926 909 570
ClaI AT'CG_AT
Cuts at: 1778 2113 1778
Size: 335 5070
DraI TTT'AAA
Cuts at: 1035 1047 2404 1035
Size: 12 1357 4036
Fragments arranged by size:
4036 1357 12
DrdI GACnn_nn'nnGTC
Cuts at: 810 3111 3969 810
Size: 2301 858 2246
Fragments arranged by size:
2301 2246 858
EaeI y'GGCC_r
Cuts at: 140 365 2990 3164 3555 3582 4927 140
Size: 225 2625 174 391 27 1345 618
Fragments arranged by size:
2625 1345 618 391 225 174 27
EagI C'GGCC_G
Cuts at: 2990 2990
Size: 5405
EarI CTCTTCn'nnn_
Cuts at: 1919 3428 3638 1919
Size: 1509 210 3686
Fragments arranged by size:
3686 1509 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1782 3613 3923 4069 5215 1782
Size: 1831 310 146 1146 1972
Fragments arranged by size:
1972 1831 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2417 2417
Size: 5405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1284 2078 3230 3662 4409 4677 1284
Size: 794 1152 432 747 268 2012
Fragments arranged by size:
2012 1152 794 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1613 2028 2103 2544 1613
Size: 415 75 441 4474
Fragments arranged by size:
4474 441 415 75
EcoRI G'AATT_C
Cuts at: 985 1805 2409 985
Size: 820 604 3981
Fragments arranged by size:
3981 820 604
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 979 1878 3424 979
Size: 899 1546 2960
Fragments arranged by size:
2960 1546 899
FspI TGC'GCA
Cuts at: 3186 3186
Size: 5405
HaeII r_GCGC'y
Cuts at: 951 1408 2839 3087 4109 5088 951
Size: 457 1431 248 1022 979 1268
Fragments arranged by size:
1431 1268 1022 979 457 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3247 3277 3279 3309 3247
Size: 30 2 30 5343
Fragments arranged by size:
5343 30 30 2
HincII GTy'rAC
Cuts at: 402 803 1902 2444 402
Size: 401 1099 542 3363
Fragments arranged by size:
3363 1099 542 401
HindIII A'AGCT_T
Cuts at: 1826 2185 1826
Size: 359 5046
KasI G'GCGC_C
Cuts at: 1404 2835 3083 1404
Size: 1431 248 3726
Fragments arranged by size:
3726 1431 248
KpnI G_GTAC'C
Cuts at: 2425 2425
Size: 5405
MluI A'CGCG_T
Cuts at: 4838 4838
Size: 5405
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2139 4076 4260 2139
Size: 1937 184 3284
Fragments arranged by size:
3284 1937 184
MscI TGG'CCA
Cuts at: 142 3166 142
Size: 3024 2381
MslI CAynn'nnrTG
Cuts at: 484 1147 1366 3394 3521 484
Size: 663 219 2028 127 2368
Fragments arranged by size:
2368 2028 663 219 127
MspA1I CmG'CkG
Cuts at: 2516 2830 3190 4203 4448 2516
Size: 314 360 1013 245 3473
Fragments arranged by size:
3473 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3586 3586
Size: 5405
NarI GG'CG_CC
Cuts at: 1405 2836 3084 1405
Size: 1431 248 3726
Fragments arranged by size:
3726 1431 248
NgoMIV G'CCGG_C
Cuts at: 3584 3584
Size: 5405
NsiI A_TGCA'T
Cuts at: 432 432
Size: 5405
NspI r_CATG'y
Cuts at: 2458 2958 3865 2458
Size: 500 907 3998
Fragments arranged by size:
3998 907 500
PciI A'CATG_T
Cuts at: 3861 3861
Size: 5405
PflMI CCAn_nnn'nTGG
Cuts at: 418 1216 418
Size: 798 4607
PfoI T'CCnGG_A
Cuts at: 1074 1074
Size: 5405
PmeI GTTT'AAAC
Cuts at: 2404 2404
Size: 5405
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4544 4576 4544
Size: 32 5373
PpuMI rG'GwC_Cy
Cuts at: 1613 2028 1613
Size: 415 4990
PstI C_TGCA'G
Cuts at: 1853 2452 3137 1853
Size: 599 685 4121
Fragments arranged by size:
4121 685 599
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5405
PvuII CAG'CTG
Cuts at: 2830 3190 2830
Size: 360 5045
RsrII CG'GwC_CG
Cuts at: 3600 3600
Size: 5405
SacI G_AGCT'C
Cuts at: 2419 2419
Size: 5405
SalI G'TCGA_C
Cuts at: 400 2442 400
Size: 2042 3363
SapI GCTCTTCn'nnn_
Cuts at: 1919 3428 3638 1919
Size: 1509 210 3686
Fragments arranged by size:
3686 1509 210
SbfI CC_TGCA'GG
Cuts at: 2452 2452
Size: 5405
ScaI AGT'ACT
Cuts at: 845 1290 1952 2289 4922 845
Size: 445 662 337 2633 1328
Fragments arranged by size:
2633 1328 662 445 337
SexAI A'CCwGG_T
Cuts at: 890 890
Size: 5405
SfcI C'TryA_G
Cuts at: 33 1849 2448 3133 4126 4317 33
Size: 1816 599 685 993 191 1121
Fragments arranged by size:
1816 1121 993 685 599 191
SfoI GGC'GCC
Cuts at: 1406 2837 3085 1406
Size: 1431 248 3726
Fragments arranged by size:
3726 1431 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5405
SmaI CCC'GGG
Cuts at: 2427 2427
Size: 5405
SmlI C'TyrA_G
Cuts at: 2560 3475 3967 4229 2560
Size: 915 492 262 3736
Fragments arranged by size:
3736 915 492 262
SnaBI TAC'GTA
Cuts at: 2362 2362
Size: 5405
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5405
SphI G_CATG'C
Cuts at: 2458 2958 2458
Size: 500 4905
SspI AAT'ATT
Cuts at: 882 1647 2231 882
Size: 765 584 4056
Fragments arranged by size:
4056 765 584
StuI AGG'CCT
Cuts at: 1568 1568
Size: 5405
StyI C'CwwG_G
Cuts at: 1238 2539 2893 1238
Size: 1301 354 3750
Fragments arranged by size:
3750 1301 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2988 3121 3580 3789 2988
Size: 133 459 209 4604
Fragments arranged by size:
4604 459 209 133
TatI w'GTAC_w
Cuts at: 843 1288 1894 1950 2287 4920 843
Size: 445 606 56 337 2633 1328
Fragments arranged by size:
2633 1328 606 445 337 56
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 695 727 4922 4954 695
Size: 32 4195 32 1146
Fragments arranged by size:
4195 1146 32 32
Tth111I GACn'n_nGTC
Cuts at: 3202 3202
Size: 5405
XbaI T'CTAG_A
Cuts at: 2436 2436
Size: 5405
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 682 2900 682
Size: 2218 3187
XmaI C'CCGG_G
Cuts at: 2425 2425
Size: 5405
XmnI GAAnn'nnTTC
Cuts at: 1717 1717
Size: 5405
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmtI Bpu10I BsaXI
BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI PacI PasI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SrfI SwaI XhoI ZraI