Restriction map for
pF1KB6246
(Circular) (Six-base) MAPSORT of: pF1KB6246.seq Check: 4293 from: 1 to: 5153
pF1KB6246 5153 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 396 578 695 1137 1223 2004 396
Size: 182 117 442 86 781 3545
Fragments arranged by size:
3545 781 442 182 117 86
AatII G_ACGT'C
Cuts at: 1505 1505
Size: 5153
AccI GT'mk_AC
Cuts at: 2191 2191
Size: 5153
Acc65I G'GTAC_C
Cuts at: 2169 2169
Size: 5153
AclI AA'CG_TT
Cuts at: 4993 4993
Size: 5153
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1230 2978 3410 4157 4425 1230
Size: 1748 432 747 268 1958
Fragments arranged by size:
1958 1748 747 432 268
AflIII A'CryG_T
Cuts at: 2505 3609 4586 2505
Size: 1104 977 3072
Fragments arranged by size:
3072 1104 977
AgeI A'CCGG_T
Cuts at: 3541 3541
Size: 5153
AleI CACnn'nnGTG
Cuts at: 1777 1777
Size: 5153
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 789 1924 789
Size: 1135 4018
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1876 1908 1876
Size: 32 5121
AlwNI CAG_nnn'CTG
Cuts at: 336 471 657 687 711 1161 1257 1360 2458
Size: 135 186 30 24 450 96 103 1098
Cuts at: 2458 4025 336
Size: 1567 1464
Fragments arranged by size:
1567 1464 1098 450 186 135 103 96
30 24
ApaI G_GGCC'C
Cuts at: 746 1417 746
Size: 671 4482
ApaLI G'TGCA_C
Cuts at: 1333 3923 1333
Size: 2590 2563
ApoI r'AATT_y
Cuts at: 2157 4506 4762 2157
Size: 2349 256 2548
Fragments arranged by size:
2548 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5153
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5153
AvaI C'yCGr_G
Cuts at: 738 817 2056 2091 2173 738
Size: 79 1239 35 82 3718
Fragments arranged by size:
3718 1239 82 79 35
BamHI G'GATC_C
Cuts at: 2178 2178
Size: 5153
BanI G'GyrC_C
Cuts at: 408 811 1705 1933 2071 2169 2583 2831 2866
Size: 403 894 228 138 98 414 248 35
Cuts at: 2866 408
Size: 2695
Fragments arranged by size:
2695 894 414 403 248 228 138 98
35
BanII G_rGCy'C
Cuts at: 364 746 1238 1393 1417 1771 2167 3197 364
Size: 382 492 155 24 354 396 1030 2320
Fragments arranged by size:
2320 1030 492 396 382 354 155 24
BbeI G_GCGC'C
Cuts at: 2075 2587 2835 2075
Size: 512 248 4393
Fragments arranged by size:
4393 512 248
BbsI GAAGACnn'nnnn_
Cuts at: 1070 2051 2111 1070
Size: 981 60 4112
Fragments arranged by size:
4112 981 60
BbvCI CC'TCA_GC
Cuts at: 1663 1801 1915 1663
Size: 138 114 4901
Fragments arranged by size:
4901 138 114
BciVI GTATCCnnnnn_n'
Cuts at: 3051 3812 5153 3051
Size: 761 1341 3051
Fragments arranged by size:
3051 1341 761
BclI T'GATC_A
Cuts at: 2673 2673
Size: 5153
BglI GCCn_nnn'nGGC
Cuts at: 1207 1840 1207
Size: 633 4520
BglII A'GATC_T
Cuts at: 1583 2668 4705 1583
Size: 1085 2037 2031
Fragments arranged by size:
2037 2031 1085
BlpI GC'TnA_GC
Cuts at: 2265 2265
Size: 5153
Bme1580I G_kGCm'C
Cuts at: 746 816 1337 1417 1938 2778 2871 3927 746
Size: 70 521 80 521 840 93 1056 1972
Fragments arranged by size:
1972 1056 840 521 521 93 80 70
BmgBI CAC'GTC
Cuts at: 1929 1929
Size: 5153
BmrI ACTGGGnnnn_n'
Cuts at: 631 1122 1989 2365 2446 2781 4925 631
Size: 491 867 376 81 335 2144 859
Fragments arranged by size:
2144 867 859 491 376 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3520 3520
Size: 5153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 508 733 1183 2137 484
Size: 24 225 450 954 3500
Fragments arranged by size:
3500 954 450 225 24
Bpu10I CC'TnA_GC
Cuts at: 358 1663 1801 1915 358
Size: 1305 138 114 3596
Fragments arranged by size:
3596 1305 138 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2329 3208 3700 3998 2329
Size: 879 492 298 3484
Fragments arranged by size:
3484 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 915 988 1045 1333 1740 4857 915
Size: 73 57 288 407 3117 1211
Fragments arranged by size:
3117 1211 407 288 73 57
BsaAI yAC'GTr
Cuts at: 2506 2506
Size: 5153
BsaBI GATnn'nnATC
Cuts at: 6 2667 4704 6
Size: 2661 2037 455
Fragments arranged by size:
2661 2037 455
BsaHI Gr'CG_yC
Cuts at: 1502 2072 2584 2832 3534 5030 1502
Size: 570 512 248 702 1496 1625
Fragments arranged by size:
1625 1496 702 570 512 248
BsaWI w'CCGG_w
Cuts at: 1115 2100 2342 2863 3541 3815 3962 4373 1115
Size: 985 242 521 678 274 147 411 1895
Fragments arranged by size:
1895 985 678 521 411 274 242 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 299 329 1419 1449 1876 1906 299
Size: 30 1090 30 427 30 3546
Fragments arranged by size:
3546 1090 427 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 568 1006 1266 1269 1449 1852 1967 2038 2134
Size: 438 260 3 180 403 115 71 96
Cuts at: 2134 2137 568
Size: 3 3584
Fragments arranged by size:
3584 438 403 260 180 115 96 71
3 3
BseYI C'CCAG_C
Cuts at: 584 595 791 1416 1954 2083 2578 3336 3913
Size: 11 196 625 538 129 495 758 577
Cuts at: 3913 4409 584
Size: 496 1328
Fragments arranged by size:
1328 758 625 577 538 496 495 196
129 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 277 424 1174 1201 1654 2032 275
Size: 2 147 750 27 453 378 3396
Fragments arranged by size:
3396 750 453 378 147 27 2
BsiEI CG_ry'CG
Cuts at: 104 875 1352 2695 2741 3949 104
Size: 771 477 1343 46 1208 1308
Fragments arranged by size:
1343 1308 1208 771 477 46
BsiHKAI G_wGCw'C
Cuts at: 364 962 1103 1337 1815 2167 2945 3135 3927
Size: 598 141 234 478 352 778 190 792
Cuts at: 3927 364
Size: 1590
Fragments arranged by size:
1590 792 778 598 478 352 234 190
141
BsmBI CGTCTCn'nnnn_
Cuts at: 1305 1305
Size: 5153
Bsp1286I G_dGCh'C
Cuts at: 364 746 816 962 1103 1238 1337 1393 1417
Size: 382 70 146 141 135 99 56 24
Cuts at: 1417 1771 1815 1938 2167 2778 2871 2945 3135
Size: 354 44 123 229 611 93 74 190
Cuts at: 3135 3197 3927 364
Size: 62 730 1590
Fragments arranged by size:
1590 730 611 382 354 229 190 146
141 135 123 99 93 74 70 62
56 44 24
BspEI T'CCGG_A
Cuts at: 2100 4373 2100
Size: 2273 2880
BspHI T'CATG_A
Cuts at: 1479 4329 1479
Size: 2850 2303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 396 578 695 704 794 1137 1223 2004 2203
Size: 182 117 9 90 343 86 781 199
Cuts at: 2203 2417 2719 3100 396
Size: 214 302 381 2449
Fragments arranged by size:
2449 781 381 343 302 214 199 182
117 90 86 9
BsrBI CCG'CTC
Cuts at: 50 898 1191 1728 3445 3499 4983 5148 50
Size: 848 293 537 1717 54 1484 165 55
Fragments arranged by size:
1717 1484 848 537 293 165 55 54
BsrDI GCAATG_nn'
Cuts at: 270 3065 270
Size: 2795 2358
BsrFI r'CCGG_y
Cuts at: 3151 3332 3541 3151
Size: 181 209 4763
Fragments arranged by size:
4763 209 181
BssSI C'ACGA_G
Cuts at: 988 1330 3424 3782 988
Size: 342 2094 358 2359
Fragments arranged by size:
2359 2094 358 342
BstAPI GCAn_nnn'nTGC
Cuts at: 336 459 495 657 672 687 1152 1819 2530
Size: 123 36 162 15 15 465 667 711
Cuts at: 2530 336
Size: 2959
Fragments arranged by size:
2959 711 667 465 162 123 36 15
15
BstBI TT'CG_AA
Cuts at: 3514 3514
Size: 5153
BstEII G'GTnAC_C
Cuts at: 1293 1293
Size: 5153
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 5153
BstYI r'GATC_y
Cuts at: 1 467 1166 1583 2178 2645 2668 3003 3249
Size: 466 699 417 595 467 23 335 246
Cuts at: 3249 4261 4347 4359 4705 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 699 595 467 466 449 417 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 351 351
Size: 5153
BtgI C'CryG_G
Cuts at: 248 1940 248
Size: 1692 3461
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3103 3284 3447 3103
Size: 181 163 4809
Fragments arranged by size:
4809 181 163
BtsI GCAGTG_nn'
Cuts at: 1440 1628 2396 1440
Size: 188 768 4197
Fragments arranged by size:
4197 768 188
DraI TTT'AAA
Cuts at: 2152 2152
Size: 5153
DraIII CAC_nnn'GTG
Cuts at: 2010 2010
Size: 5153
DrdI GACnn_nn'nnGTC
Cuts at: 138 2859 3717 138
Size: 2721 858 1574
Fragments arranged by size:
2721 1574 858
EaeI y'GGCC_r
Cuts at: 422 872 1039 1349 1841 2738 2912 3303 3330
Size: 450 167 310 492 897 174 391 27
Cuts at: 3330 4675 422
Size: 1345 900
Fragments arranged by size:
1345 900 897 492 450 391 310 174
167 27
EagI C'GGCC_G
Cuts at: 872 1349 2738 872
Size: 477 1389 3287
Fragments arranged by size:
3287 1389 477
EarI CTCTTCn'nnn_
Cuts at: 987 3176 3386 987
Size: 2189 210 2754
Fragments arranged by size:
2754 2189 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1525 1884 3361 3671 3817 4963 1525
Size: 359 1477 310 146 1146 1715
Fragments arranged by size:
1715 1477 1146 359 310 146
EcoICRI GAG'CTC
Cuts at: 362 2165 362
Size: 1803 3350
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 508 733 1183 1230 2137 2978 3410 4157
Size: 24 225 450 47 907 841 432 747
Cuts at: 4157 4425 484
Size: 268 1212
Fragments arranged by size:
1212 907 841 747 450 432 268 225
47 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2119 2119
Size: 5153
EcoO109I rG'GnC_Cy
Cuts at: 742 743 1413 2095 2112 2292 742
Size: 1 670 682 17 180 3603
Fragments arranged by size:
3603 682 670 180 17 1
EcoRI G'AATT_C
Cuts at: 2157 2157
Size: 5153
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3172 3172
Size: 5153
FspI TGC'GCA
Cuts at: 2934 2934
Size: 5153
HaeII r_GCGC'y
Cuts at: 2075 2090 2587 2835 3857 4836 2075
Size: 15 497 248 1022 979 2392
Fragments arranged by size:
2392 1022 979 497 248 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2995 3025 3027 3057 2995
Size: 30 2 30 5091
Fragments arranged by size:
5091 30 30 2
HincII GTy'rAC
Cuts at: 2192 2192
Size: 5153
KasI G'GCGC_C
Cuts at: 2071 2583 2831 2071
Size: 512 248 4393
Fragments arranged by size:
4393 512 248
KpnI G_GTAC'C
Cuts at: 2173 2173
Size: 5153
MluI A'CGCG_T
Cuts at: 4586 4586
Size: 5153
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1405 3824 4008 1405
Size: 2419 184 2550
Fragments arranged by size:
2550 2419 184
MscI TGG'CCA
Cuts at: 424 1041 2914 424
Size: 617 1873 2663
Fragments arranged by size:
2663 1873 617
MslI CAynn'nnrTG
Cuts at: 112 1603 1777 3142 3269 112
Size: 1491 174 1365 127 1996
Fragments arranged by size:
1996 1491 1365 174 127
MspA1I CmG'CkG
Cuts at: 200 486 636 769 795 1161 1569 1950 2264
Size: 286 150 133 26 366 408 381 314
Cuts at: 2264 2578 2938 3951 4196 200
Size: 314 360 1013 245 1157
Fragments arranged by size:
1157 1013 408 381 366 360 314 314
286 245 150 133 26
NaeI GCC'GGC
Cuts at: 3334 3334
Size: 5153
NarI GG'CG_CC
Cuts at: 2072 2584 2832 2072
Size: 512 248 4393
Fragments arranged by size:
4393 512 248
NgoMIV G'CCGG_C
Cuts at: 3332 3332
Size: 5153
NspI r_CATG'y
Cuts at: 1224 1881 2206 2706 3613 1224
Size: 657 325 500 907 2764
Fragments arranged by size:
2764 907 657 500 325
PasI CC'CwG_GG
Cuts at: 367 2067 367
Size: 1700 3453
PciI A'CATG_T
Cuts at: 3609 3609
Size: 5153
PfoI T'CCnGG_A
Cuts at: 1547 1547
Size: 5153
PmeI GTTT'AAAC
Cuts at: 2152 2152
Size: 5153
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1777 1809 1876 1908 4292 4324 1777
Size: 32 67 32 2384 32 2606
Fragments arranged by size:
2606 2384 67 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2112 2112
Size: 5153
PspOMI G'GGCC_C
Cuts at: 742 1413 742
Size: 671 4482
PstI C_TGCA'G
Cuts at: 344 491 575 701 725 1199 1308 2200 2885
Size: 147 84 126 24 474 109 892 685
Cuts at: 2885 344
Size: 2612
Fragments arranged by size:
2612 892 685 474 147 126 109 84
24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5153
PvuII CAG'CTG
Cuts at: 486 636 769 795 1161 1569 2578 2938 486
Size: 150 133 26 366 408 1009 360 2701
Fragments arranged by size:
2701 1009 408 366 360 150 133 26
RsrII CG'GwC_CG
Cuts at: 3348 3348
Size: 5153
SacI G_AGCT'C
Cuts at: 364 2167 364
Size: 1803 3350
SalI G'TCGA_C
Cuts at: 2190 2190
Size: 5153
SapI GCTCTTCn'nnn_
Cuts at: 987 3176 3386 987
Size: 2189 210 2754
Fragments arranged by size:
2754 2189 210
SbfI CC_TGCA'GG
Cuts at: 2200 2200
Size: 5153
ScaI AGT'ACT
Cuts at: 541 4670 541
Size: 4129 1024
SexAI A'CCwGG_T
Cuts at: 2006 2006
Size: 5153
SfcI C'TryA_G
Cuts at: 33 340 487 532 571 697 721 1195 1304
Size: 307 147 45 39 126 24 474 109
Cuts at: 1304 2196 2881 3874 4065 33
Size: 892 685 993 191 1121
Fragments arranged by size:
1121 993 892 685 474 307 191 147
126 109 45 39 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 1207 1840 1207
Size: 633 4520
SfoI GGC'GCC
Cuts at: 2073 2585 2833 2073
Size: 512 248 4393
Fragments arranged by size:
4393 512 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5153
SmaI CCC'GGG
Cuts at: 819 2058 2175 819
Size: 1239 117 3797
Fragments arranged by size:
3797 1239 117
SmlI C'TyrA_G
Cuts at: 2308 3223 3715 3977 2308
Size: 915 492 262 3484
Fragments arranged by size:
3484 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5153
SphI G_CATG'C
Cuts at: 1881 2206 2706 1881
Size: 325 500 4328
Fragments arranged by size:
4328 500 325
SrfI GCCC'GGGC
Cuts at: 2058 2058
Size: 5153
StuI AGG'CCT
Cuts at: 349 356 493 349
Size: 7 137 5009
Fragments arranged by size:
5009 137 7
StyI C'CwwG_G
Cuts at: 905 1178 1289 2287 2641 905
Size: 273 111 998 354 3417
Fragments arranged by size:
3417 998 354 273 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 598 1921 2736 2869 3328 3537 598
Size: 1323 815 133 459 209 2214
Fragments arranged by size:
2214 1323 815 459 209 133
TatI w'GTAC_w
Cuts at: 539 1120 1997 4668 539
Size: 581 877 2671 1024
Fragments arranged by size:
2671 1024 877 581
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1419 1451 4670 4702 1419
Size: 32 3219 32 1870
Fragments arranged by size:
3219 1870 32 32
Tth111I GACn'n_nGTC
Cuts at: 838 2950 838
Size: 2112 3041
XbaI T'CTAG_A
Cuts at: 2184 2184
Size: 5153
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 592 618 2648 592
Size: 26 2030 3097
Fragments arranged by size:
3097 2030 26
XmaI C'CCGG_G
Cuts at: 817 2056 2173 817
Size: 1239 117 3797
Fragments arranged by size:
3797 1239 117
ZraI GAC'GTC
Cuts at: 1503 1503
Size: 5153
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AfeI AflII AhdI AscI AvrII BaeI BcgI BfrBI
BmtI BsiWI BsmI BsrGI BssHII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SgrAI SnaBI SspI
SwaI XhoI XmnI