Restriction map for
pF1KB6238
(Circular) (Six-base) MAPSORT of: pF1KB6238.seq Check: 5862 from: 1 to: 5144
pF1KB6238 5144 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AatII G_ACGT'C
Cuts at: 494 494
Size: 5144
AccI GT'mk_AC
Cuts at: 2182 2182
Size: 5144
Acc65I G'GTAC_C
Cuts at: 2160 2160
Size: 5144
AclI AA'CG_TT
Cuts at: 4984 4984
Size: 5144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 605 622 661 751 1648 2969 3401 4148
Size: 457 17 39 90 897 1321 432 747
Cuts at: 4148 4416 148
Size: 268 876
Fragments arranged by size:
1321 897 876 747 457 432 268 90
39 17
AfeI AGC'GCT
Cuts at: 971 971
Size: 5144
AflIII A'CryG_T
Cuts at: 2496 3600 4577 2496
Size: 1104 977 3063
Fragments arranged by size:
3063 1104 977
AgeI A'CCGG_T
Cuts at: 3532 3532
Size: 5144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 160 860 160
Size: 700 4444
AlwNI CAG_nnn'CTG
Cuts at: 873 1665 1767 2449 4016 873
Size: 792 102 682 1567 2001
Fragments arranged by size:
2001 1567 792 682 102
ApaI G_GGCC'C
Cuts at: 720 1478 1512 720
Size: 758 34 4352
Fragments arranged by size:
4352 758 34
ApaLI G'TGCA_C
Cuts at: 3914 3914
Size: 5144
ApoI r'AATT_y
Cuts at: 1459 2148 4497 4753 1459
Size: 689 2349 256 1850
Fragments arranged by size:
2349 1850 689 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5144
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5144
AvaI C'yCGr_G
Cuts at: 476 544 1505 2164 476
Size: 68 961 659 3456
Fragments arranged by size:
3456 961 659 68
BamHI G'GATC_C
Cuts at: 1313 2169 1313
Size: 856 4288
BanI G'GyrC_C
Cuts at: 480 696 2160 2574 2822 2857 480
Size: 216 1464 414 248 35 2767
Fragments arranged by size:
2767 1464 414 248 216 35
BanII G_rGCy'C
Cuts at: 720 808 1478 1512 2158 3188 720
Size: 88 670 34 646 1030 2676
Fragments arranged by size:
2676 1030 670 646 88 34
BbeI G_GCGC'C
Cuts at: 700 2578 2826 700
Size: 1878 248 3018
Fragments arranged by size:
3018 1878 248
BbsI GAAGACnn'nnnn_
Cuts at: 635 1213 635
Size: 578 4566
BbvCI CC'TCA_GC
Cuts at: 113 1774 2126 113
Size: 1661 352 3131
Fragments arranged by size:
3131 1661 352
BciVI GTATCCnnnnn_n'
Cuts at: 3042 3803 5144 3042
Size: 761 1341 3042
Fragments arranged by size:
3042 1341 761
BclI T'GATC_A
Cuts at: 2664 2664
Size: 5144
BglI GCCn_nnn'nGGC
Cuts at: 823 1453 823
Size: 630 4514
BglII A'GATC_T
Cuts at: 1124 2659 4696 1124
Size: 1535 2037 1572
Fragments arranged by size:
2037 1572 1535
BlpI GC'TnA_GC
Cuts at: 133 514 1250 1342 1529 2256 133
Size: 381 736 92 187 727 3021
Fragments arranged by size:
3021 736 727 381 187 92
Bme1580I G_kGCm'C
Cuts at: 485 720 1092 1478 1512 2034 2769 2862 3918
Size: 235 372 386 34 522 735 93 1056
Cuts at: 3918 485
Size: 1711
Fragments arranged by size:
1711 1056 735 522 386 372 235 93
34
BmrI ACTGGGnnnn_n'
Cuts at: 1095 2356 2437 2772 4916 1095
Size: 1261 81 335 2144 1323
Fragments arranged by size:
2144 1323 1261 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3511 3511
Size: 5144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 1725 1970 2057 2063 730
Size: 995 245 87 6 3811
Fragments arranged by size:
3811 995 245 87 6
Bpu10I CC'TnA_GC
Cuts at: 113 1774 1794 2126 113
Size: 1661 20 332 3131
Fragments arranged by size:
3131 1661 332 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2320 3199 3691 3989 2320
Size: 879 492 298 3475
Fragments arranged by size:
3475 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 202 1866 1938 4848 202
Size: 1664 72 2910 498
Fragments arranged by size:
2910 1664 498 72
BsaAI yAC'GTr
Cuts at: 2497 2497
Size: 5144
BsaBI GATnn'nnATC
Cuts at: 6 2658 4695 6
Size: 2652 2037 455
Fragments arranged by size:
2652 2037 455
BsaHI Gr'CG_yC
Cuts at: 491 697 1210 1445 2575 2823 3525 5021 491
Size: 206 513 235 1130 248 702 1496 614
Fragments arranged by size:
1496 1130 702 614 513 248 235 206
BsaWI w'CCGG_w
Cuts at: 1355 2136 2333 2854 3532 3806 3953 4364 1355
Size: 781 197 521 678 274 147 411 2135
Fragments arranged by size:
2135 781 678 521 411 274 197 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2104 2134 2104
Size: 30 5114
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 220 445 1471 1697 130
Size: 90 225 1026 226 3577
Fragments arranged by size:
3577 1026 226 225 90
BseYI C'CCAG_C
Cuts at: 558 1193 1254 1757 1834 1885 2056 2105 2569
Size: 635 61 503 77 51 171 49 464
Cuts at: 2569 3327 3904 4400 558
Size: 758 577 496 1302
Fragments arranged by size:
1302 758 635 577 503 496 464 171
77 61 51 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1396 1658 1861 1396
Size: 262 203 4679
Fragments arranged by size:
4679 262 203
BsiEI CG_ry'CG
Cuts at: 104 2686 2732 3940 104
Size: 2582 46 1208 1308
Fragments arranged by size:
2582 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1404 2158 2936 3126 3918 1404
Size: 754 778 190 792 2630
Fragments arranged by size:
2630 792 778 754 190
Bsp1286I G_dGCh'C
Cuts at: 485 720 808 1092 1404 1478 1512 2034 2158
Size: 235 88 284 312 74 34 522 124
Cuts at: 2158 2769 2862 2936 3126 3188 3918 485
Size: 611 93 74 190 62 730 1711
Fragments arranged by size:
1711 730 611 522 312 284 235 190
124 93 88 74 74 62 34
BspEI T'CCGG_A
Cuts at: 4364 4364
Size: 5144
BspHI T'CATG_A
Cuts at: 4320 4320
Size: 5144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 220 1045 1524 1752 2194 2408 2710 3091 220
Size: 825 479 228 442 214 302 381 2273
Fragments arranged by size:
2273 825 479 442 381 302 228 214
BsrBI CCG'CTC
Cuts at: 50 1311 1681 3436 3490 4974 5139 50
Size: 1261 370 1755 54 1484 165 55
Fragments arranged by size:
1755 1484 1261 370 165 55 54
BsrDI GCAATG_nn'
Cuts at: 842 3056 842
Size: 2214 2930
BsrFI r'CCGG_y
Cuts at: 3142 3323 3532 3142
Size: 181 209 4754
Fragments arranged by size:
4754 209 181
BssSI C'ACGA_G
Cuts at: 748 1676 3415 3773 748
Size: 928 1739 358 2119
Fragments arranged by size:
2119 1739 928 358
BstAPI GCAn_nnn'nTGC
Cuts at: 265 2521 265
Size: 2256 2888
BstBI TT'CG_AA
Cuts at: 3505 3505
Size: 5144
BstXI CCAn_nnnn'nTGG
Cuts at: 168 168
Size: 5144
BstYI r'GATC_y
Cuts at: 1 1124 1313 2169 2636 2659 2994 3240 4252
Size: 1123 189 856 467 23 335 246 1012
Cuts at: 4252 4338 4350 4696 1
Size: 86 12 346 449
Fragments arranged by size:
1123 1012 856 467 449 346 335 246
189 86 23 12
BtgI C'CryG_G
Cuts at: 107 182 848 877 107
Size: 75 666 29 4374
Fragments arranged by size:
4374 666 75 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 174 1221 1629 3094 3275 3438 174
Size: 1047 408 1465 181 163 1880
Fragments arranged by size:
1880 1465 1047 408 181 163
BtsI GCAGTG_nn'
Cuts at: 1326 1889 2034 2387 1326
Size: 563 145 353 4083
Fragments arranged by size:
4083 563 353 145
DraI TTT'AAA
Cuts at: 2143 2143
Size: 5144
DrdI GACnn_nn'nnGTC
Cuts at: 1668 2850 3708 1668
Size: 1182 858 3104
Fragments arranged by size:
3104 1182 858
EaeI y'GGCC_r
Cuts at: 2729 2903 3294 3321 4666 2729
Size: 174 391 27 1345 3207
Fragments arranged by size:
3207 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2729 2729
Size: 5144
EarI CTCTTCn'nnn_
Cuts at: 3167 3377 3167
Size: 210 4934
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 841 3352 3662 3808 4954 841
Size: 2511 310 146 1146 1031
Fragments arranged by size:
2511 1146 1031 310 146
EcoICRI GAG'CTC
Cuts at: 2156 2156
Size: 5144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 605 622 661 730 751 1648 1725 1970
Size: 457 17 39 69 21 897 77 245
Cuts at: 1970 2057 2063 2969 3401 4148 4416 148
Size: 87 6 906 432 747 268 876
Fragments arranged by size:
906 897 876 747 457 432 268 245
87 77 69 39 21 17 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1588 1588
Size: 5144
EcoO109I rG'GnC_Cy
Cuts at: 716 717 1466 1475 1593 1764 1966 2283 716
Size: 1 749 9 118 171 202 317 3577
Fragments arranged by size:
3577 749 317 202 171 118 9 1
EcoRI G'AATT_C
Cuts at: 1459 2148 1459
Size: 689 4455
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3163 3163
Size: 5144
FspI TGC'GCA
Cuts at: 2925 2925
Size: 5144
HaeII r_GCGC'y
Cuts at: 700 973 1748 2578 2826 3848 4827 700
Size: 273 775 830 248 1022 979 1017
Fragments arranged by size:
1022 1017 979 830 775 273 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2986 3016 3018 3048 2986
Size: 30 2 30 5082
Fragments arranged by size:
5082 30 30 2
HincII GTy'rAC
Cuts at: 1539 2183 1539
Size: 644 4500
HindIII A'AGCT_T
Cuts at: 130 130
Size: 5144
KasI G'GCGC_C
Cuts at: 696 2574 2822 696
Size: 1878 248 3018
Fragments arranged by size:
3018 1878 248
KpnI G_GTAC'C
Cuts at: 2164 2164
Size: 5144
MluI A'CGCG_T
Cuts at: 4577 4577
Size: 5144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1735 3815 3999 1735
Size: 2080 184 2880
Fragments arranged by size:
2880 2080 184
MscI TGG'CCA
Cuts at: 2905 2905
Size: 5144
MslI CAynn'nnrTG
Cuts at: 166 283 3133 3260 166
Size: 117 2850 127 2050
Fragments arranged by size:
2850 2050 127 117
MspA1I CmG'CkG
Cuts at: 219 1027 2024 2255 2569 2929 3942 4187 219
Size: 808 997 231 314 360 1013 245 1176
Fragments arranged by size:
1176 1013 997 808 360 314 245 231
NaeI GCC'GGC
Cuts at: 3325 3325
Size: 5144
NarI GG'CG_CC
Cuts at: 697 2575 2823 697
Size: 1878 248 3018
Fragments arranged by size:
3018 1878 248
NcoI C'CATG_G
Cuts at: 107 182 107
Size: 75 5069
NgoMIV G'CCGG_C
Cuts at: 3323 3323
Size: 5144
NspI r_CATG'y
Cuts at: 2197 2697 3604 2197
Size: 500 907 3737
Fragments arranged by size:
3737 907 500
PciI A'CATG_T
Cuts at: 3600 3600
Size: 5144
PflMI CCAn_nnn'nTGG
Cuts at: 358 1193 1603 1665 358
Size: 835 410 62 3837
Fragments arranged by size:
3837 835 410 62
PfoI T'CCnGG_A
Cuts at: 1165 1165
Size: 5144
PmeI GTTT'AAAC
Cuts at: 2143 2143
Size: 5144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4283 4315 4283
Size: 32 5112
PpuMI rG'GwC_Cy
Cuts at: 1466 1764 1466
Size: 298 4846
PspOMI G'GGCC_C
Cuts at: 716 1474 1508 716
Size: 758 34 4352
Fragments arranged by size:
4352 758 34
PstI C_TGCA'G
Cuts at: 1298 1526 2191 2876 1298
Size: 228 665 685 3566
Fragments arranged by size:
3566 685 665 228
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5144
PvuII CAG'CTG
Cuts at: 219 1027 2569 2929 219
Size: 808 1542 360 2434
Fragments arranged by size:
2434 1542 808 360
RsrII CG'GwC_CG
Cuts at: 3339 3339
Size: 5144
SacI G_AGCT'C
Cuts at: 2158 2158
Size: 5144
SalI G'TCGA_C
Cuts at: 2181 2181
Size: 5144
SanDI GG'GwC_CC
Cuts at: 1466 1466
Size: 5144
SapI GCTCTTCn'nnn_
Cuts at: 3167 3377 3167
Size: 210 4934
SbfI CC_TGCA'GG
Cuts at: 2191 2191
Size: 5144
ScaI AGT'ACT
Cuts at: 4661 4661
Size: 5144
SfcI C'TryA_G
Cuts at: 33 1294 1522 2187 2872 3865 4056 33
Size: 1261 228 665 685 993 191 1121
Fragments arranged by size:
1261 1121 993 685 665 228 191
SfoI GGC'GCC
Cuts at: 698 2576 2824 698
Size: 1878 248 3018
Fragments arranged by size:
3018 1878 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5144
SmaI CCC'GGG
Cuts at: 478 2166 478
Size: 1688 3456
SmlI C'TyrA_G
Cuts at: 2299 3214 3706 3968 2299
Size: 915 492 262 3475
Fragments arranged by size:
3475 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5144
SphI G_CATG'C
Cuts at: 2197 2697 2197
Size: 500 4644
StuI AGG'CCT
Cuts at: 796 796
Size: 5144
StyI C'CwwG_G
Cuts at: 107 182 1470 2278 2632 107
Size: 75 1288 808 354 2619
Fragments arranged by size:
2619 1288 808 354 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1185 1771 2727 2860 3319 3528 1185
Size: 586 956 133 459 209 2801
Fragments arranged by size:
2801 956 586 459 209 133
TatI w'GTAC_w
Cuts at: 1922 4659 1922
Size: 2737 2407
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1305 1337 1640 1672 1962 1994 4661 4693 1305
Size: 32 303 32 290 32 2667 32 1756
Fragments arranged by size:
2667 1756 303 290 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2941 2941
Size: 5144
XbaI T'CTAG_A
Cuts at: 996 2175 996
Size: 1179 3965
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 168 1194 1242 1854 2639 168
Size: 1026 48 612 785 2673
Fragments arranged by size:
2673 1026 785 612 48
XmaI C'CCGG_G
Cuts at: 476 2164 476
Size: 1688 3456
ZraI GAC'GTC
Cuts at: 492 492
Size: 5144
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI