Restriction map for
pF1KB6229
(Circular) (Six-base) MAPSORT of: pF1KB6229.seq Check: 4390 from: 1 to: 5132
pF1KB6229 5132 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 777 837 1011 1724 777
Size: 60 174 713 4185
Fragments arranged by size:
4185 713 174 60
AccI GT'mk_AC
Cuts at: 2170 2170
Size: 5132
Acc65I G'GTAC_C
Cuts at: 560 2148 560
Size: 1588 3544
AclI AA'CG_TT
Cuts at: 4972 4972
Size: 5132
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 647 745 1194 2957 3389 4136 4404 484
Size: 163 98 449 1763 432 747 268 1212
Fragments arranged by size:
1763 1212 747 449 432 268 163 98
AfeI AGC'GCT
Cuts at: 1600 1936 1600
Size: 336 4796
AflIII A'CryG_T
Cuts at: 2484 3588 4565 2484
Size: 1104 977 3051
Fragments arranged by size:
3051 1104 977
AgeI A'CCGG_T
Cuts at: 3520 3520
Size: 5132
AleI CACnn'nnGTG
Cuts at: 1814 1898 1814
Size: 84 5048
AlwNI CAG_nnn'CTG
Cuts at: 357 462 2437 4004 357
Size: 105 1975 1567 1485
Fragments arranged by size:
1975 1567 1485 105
ApaI G_GGCC'C
Cuts at: 644 779 644
Size: 135 4997
ApaLI G'TGCA_C
Cuts at: 1508 1891 2001 3902 1508
Size: 383 110 1901 2738
Fragments arranged by size:
2738 1901 383 110
ApoI r'AATT_y
Cuts at: 2136 4485 4741 2136
Size: 2349 256 2527
Fragments arranged by size:
2527 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5132
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5132
AvaI C'yCGr_G
Cuts at: 778 1940 2068 2152 778
Size: 1162 128 84 3758
Fragments arranged by size:
3758 1162 128 84
BamHI G'GATC_C
Cuts at: 2157 2157
Size: 5132
BanI G'GyrC_C
Cuts at: 560 809 858 1023 1477 1984 2148 2562 2810
Size: 249 49 165 454 507 164 414 248
Cuts at: 2810 2845 560
Size: 35 2847
Fragments arranged by size:
2847 507 454 414 249 248 165 164
49 35
BanII G_rGCy'C
Cuts at: 644 661 779 1415 2146 3176 644
Size: 17 118 636 731 1030 2600
Fragments arranged by size:
2600 1030 731 636 118 17
BbeI G_GCGC'C
Cuts at: 1988 2566 2814 1988
Size: 578 248 4306
Fragments arranged by size:
4306 578 248
BbsI GAAGACnn'nnnn_
Cuts at: 838 1148 1402 1569 2107 838
Size: 310 254 167 538 3863
Fragments arranged by size:
3863 538 310 254 167
BbvCI CC'TCA_GC
Cuts at: 824 1037 824
Size: 213 4919
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1499 1533 1499
Size: 34 5098
BciVI GTATCCnnnnn_n'
Cuts at: 3030 3791 5132 3030
Size: 761 1341 3030
Fragments arranged by size:
3030 1341 761
BclI T'GATC_A
Cuts at: 1497 1958 2652 1497
Size: 461 694 3977
Fragments arranged by size:
3977 694 461
BfrBI ATG'CAT
Cuts at: 367 367
Size: 5132
BglI GCCn_nnn'nGGC
Cuts at: 517 619 517
Size: 102 5030
BglII A'GATC_T
Cuts at: 2647 4684 2647
Size: 2037 3095
BlpI GC'TnA_GC
Cuts at: 2244 2244
Size: 5132
Bme1580I G_kGCm'C
Cuts at: 644 779 863 1512 1895 2005 2757 2850 3906
Size: 135 84 649 383 110 752 93 1056
Cuts at: 3906 644
Size: 1870
Fragments arranged by size:
1870 1056 752 649 383 135 110 93
84
BmrI ACTGGGnnnn_n'
Cuts at: 2344 2425 2760 4904 2344
Size: 81 335 2144 2572
Fragments arranged by size:
2572 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3499 3499
Size: 5132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 454 469 493 1555 1894 2109 379
Size: 75 15 24 1062 339 215 3402
Fragments arranged by size:
3402 1062 339 215 75 24 15
Bpu10I CC'TnA_GC
Cuts at: 824 1037 1067 824
Size: 213 30 4889
Fragments arranged by size:
4889 213 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 1774 1846 2308 3187 3679 3977 550
Size: 1224 72 462 879 492 298 1705
Fragments arranged by size:
1705 1224 879 492 462 298 72
BsaI GGTCTCn'nnnn_
Cuts at: 469 4836 469
Size: 4367 765
BsaAI yAC'GTr
Cuts at: 1179 2485 1179
Size: 1306 3826
BsaBI GATnn'nnATC
Cuts at: 6 2646 4683 6
Size: 2640 2037 455
Fragments arranged by size:
2640 2037 455
BsaHI Gr'CG_yC
Cuts at: 1985 2563 2811 3513 5009 1985
Size: 578 248 702 1496 2108
Fragments arranged by size:
2108 1496 702 578 248
BsaWI w'CCGG_w
Cuts at: 251 2321 2842 3520 3794 3941 4352 251
Size: 2070 521 678 274 147 411 1031
Fragments arranged by size:
2070 1031 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 427 442 457 472 592 617 622 647 728
Size: 15 15 15 120 25 5 25 81
Cuts at: 728 758 1921 1951 427
Size: 30 1163 30 3608
Fragments arranged by size:
3608 1163 120 81 30 30 25 25
15 15 15 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 613 796 1021 2025 2086 613
Size: 183 225 1004 61 3659
Fragments arranged by size:
3659 1004 225 183 61
BseYI C'CCAG_C
Cuts at: 796 862 1032 1074 1269 1690 2026 2557 3315
Size: 66 170 42 195 421 336 531 758
Cuts at: 3315 3892 4388 796
Size: 577 496 1540
Fragments arranged by size:
1540 758 577 531 496 421 336 195
170 66 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 1201 1344 1366 1995 252
Size: 949 143 22 629 3389
Fragments arranged by size:
3389 949 629 143 22
BsiEI CG_ry'CG
Cuts at: 104 425 2674 2720 3928 104
Size: 321 2249 46 1208 1308
Fragments arranged by size:
2249 1308 1208 321 46
BsiHKAI G_wGCw'C
Cuts at: 1073 1415 1512 1786 1895 2005 2146 2924 3114
Size: 342 97 274 109 110 141 778 190
Cuts at: 3114 3906 1073
Size: 792 2299
Fragments arranged by size:
2299 792 778 342 274 190 141 110
109 97
BsiWI C'GTAC_G
Cuts at: 1400 1400
Size: 5132
BsmI GAATG_Cn'
Cuts at: 588 1457 588
Size: 869 4263
BsmBI CGTCTCn'nnnn_
Cuts at: 1870 1870
Size: 5132
Bsp1286I G_dGCh'C
Cuts at: 644 661 779 863 1073 1415 1512 1786 1895
Size: 17 118 84 210 342 97 274 109
Cuts at: 1895 2005 2146 2757 2850 2924 3114 3176 3906
Size: 110 141 611 93 74 190 62 730
Cuts at: 3906 644
Size: 1870
Fragments arranged by size:
1870 730 611 342 274 210 190 141
118 110 109 97 93 84 74 62
17
BspEI T'CCGG_A
Cuts at: 4352 4352
Size: 5132
BspHI T'CATG_A
Cuts at: 4308 4308
Size: 5132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 419 777 837 1011 1724 2182 2396 2698 3079
Size: 358 60 174 713 458 214 302 381
Cuts at: 3079 419
Size: 2472
Fragments arranged by size:
2472 713 458 381 358 302 214 174
60
BsrBI CCG'CTC
Cuts at: 50 3424 3478 4962 5127 50
Size: 3374 54 1484 165 55
Fragments arranged by size:
3374 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1842 3044 1842
Size: 1202 3930
BsrFI r'CCGG_y
Cuts at: 3130 3311 3520 3130
Size: 181 209 4742
Fragments arranged by size:
4742 209 181
BssSI C'ACGA_G
Cuts at: 3403 3761 3403
Size: 358 4774
BstAPI GCAn_nnn'nTGC
Cuts at: 1673 2009 2509 1673
Size: 336 500 4296
Fragments arranged by size:
4296 500 336
BstBI TT'CG_AA
Cuts at: 1519 3493 1519
Size: 1974 3158
BstYI r'GATC_y
Cuts at: 1 111 287 428 2075 2157 2624 2647 2982
Size: 110 176 141 1647 82 467 23 335
Cuts at: 2982 3228 4240 4326 4338 4684 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1647 1012 467 449 346 335 246 176
141 110 86 82 23 12
BtgI C'CryG_G
Cuts at: 107 158 515 644 1202 107
Size: 51 357 129 558 4037
Fragments arranged by size:
4037 558 357 129 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 224 3082 3263 3426 224
Size: 2858 181 163 1930
Fragments arranged by size:
2858 1930 181 163
BtsI GCAGTG_nn'
Cuts at: 719 1327 1618 1674 2375 719
Size: 608 291 56 701 3476
Fragments arranged by size:
3476 701 608 291 56
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 660 695 1824 1859 660
Size: 35 1129 35 3933
Fragments arranged by size:
3933 1129 35 35
DraI TTT'AAA
Cuts at: 2131 2131
Size: 5132
DrdI GACnn_nn'nnGTC
Cuts at: 1573 2838 3696 1573
Size: 1265 858 3009
Fragments arranged by size:
3009 1265 858
EaeI y'GGCC_r
Cuts at: 194 422 518 1044 1059 1571 1952 2717 2891
Size: 228 96 526 15 512 381 765 174
Cuts at: 2891 3282 3309 4654 194
Size: 391 27 1345 672
Fragments arranged by size:
1345 765 672 526 512 391 381 228
174 96 27 15
EagI C'GGCC_G
Cuts at: 422 2717 422
Size: 2295 2837
EarI CTCTTCn'nnn_
Cuts at: 298 536 764 3155 3365 298
Size: 238 228 2391 210 2065
Fragments arranged by size:
2391 2065 238 228 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 409 3340 3650 3796 4942 409
Size: 2931 310 146 1146 599
Fragments arranged by size:
2931 1146 599 310 146
EcoICRI GAG'CTC
Cuts at: 1413 2144 1413
Size: 731 4401
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 454 469 484 493 647 745 1194 1555
Size: 75 15 15 9 154 98 449 361
Cuts at: 1555 1894 2109 2957 3389 4136 4404 379
Size: 339 215 848 432 747 268 1107
Fragments arranged by size:
1107 848 747 449 432 361 339 268
215 154 98 75 15 15 9
EcoO109I rG'GnC_Cy
Cuts at: 399 775 943 950 1050 2271 399
Size: 376 168 7 100 1221 3260
Fragments arranged by size:
3260 1221 376 168 100 7
EcoRI G'AATT_C
Cuts at: 2136 2136
Size: 5132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 591 3151 591
Size: 2560 2572
FspI TGC'GCA
Cuts at: 1654 2913 1654
Size: 1259 3873
HaeII r_GCGC'y
Cuts at: 1602 1938 1988 2566 2814 3836 4815 1602
Size: 336 50 578 248 1022 979 1919
Fragments arranged by size:
1919 1022 979 578 336 248 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 316 348 617 649 728 760 942 974 2974
Size: 32 269 32 79 32 182 32 2000
Cuts at: 2974 3004 3006 3036 316
Size: 30 2 30 2412
Fragments arranged by size:
2412 2000 269 182 79 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 310 2171 310
Size: 1861 3271
KasI G'GCGC_C
Cuts at: 1984 2562 2810 1984
Size: 578 248 4306
Fragments arranged by size:
4306 578 248
KpnI G_GTAC'C
Cuts at: 564 2152 564
Size: 1588 3544
MluI A'CGCG_T
Cuts at: 4565 4565
Size: 5132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3803 3987 3803
Size: 184 4948
MscI TGG'CCA
Cuts at: 1046 2893 1046
Size: 1847 3285
MslI CAynn'nnrTG
Cuts at: 223 1567 1814 1898 3121 3248 223
Size: 1344 247 84 1223 127 2107
Fragments arranged by size:
2107 1344 1223 247 127 84
MspA1I CmG'CkG
Cuts at: 138 1036 1474 1766 1872 2243 2557 2917 3930
Size: 898 438 292 106 371 314 360 1013
Cuts at: 3930 4175 138
Size: 245 1095
Fragments arranged by size:
1095 1013 898 438 371 360 314 292
245 106
NaeI GCC'GGC
Cuts at: 3313 3313
Size: 5132
NarI GG'CG_CC
Cuts at: 1985 2563 2811 1985
Size: 578 248 4306
Fragments arranged by size:
4306 578 248
NcoI C'CATG_G
Cuts at: 107 515 644 1202 107
Size: 408 129 558 4037
Fragments arranged by size:
4037 558 408 129
NgoMIV G'CCGG_C
Cuts at: 3311 3311
Size: 5132
NsiI A_TGCA'T
Cuts at: 369 369
Size: 5132
NspI r_CATG'y
Cuts at: 1152 2185 2685 3592 1152
Size: 1033 500 907 2692
Fragments arranged by size:
2692 1033 907 500
PasI CC'CwG_GG
Cuts at: 637 814 877 1236 637
Size: 177 63 359 4533
Fragments arranged by size:
4533 359 177 63
PciI A'CATG_T
Cuts at: 3588 3588
Size: 5132
PfoI T'CCnGG_A
Cuts at: 431 1430 1772 431
Size: 999 342 3791
Fragments arranged by size:
3791 999 342
PmeI GTTT'AAAC
Cuts at: 2131 2131
Size: 5132
PmlI CAC'GTG
Cuts at: 1179 1179
Size: 5132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1738 1770 4271 4303 1738
Size: 32 2501 32 2567
Fragments arranged by size:
2567 2501 32 32
PpuMI rG'GwC_Cy
Cuts at: 399 950 399
Size: 551 4581
PspOMI G'GGCC_C
Cuts at: 640 775 640
Size: 135 4997
PstI C_TGCA'G
Cuts at: 143 714 752 774 1625 1669 2179 2864 143
Size: 571 38 22 851 44 510 685 2411
Fragments arranged by size:
2411 851 685 571 510 44 38 22
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5132
PvuII CAG'CTG
Cuts at: 138 1036 1766 1872 2557 2917 138
Size: 898 730 106 685 360 2353
Fragments arranged by size:
2353 898 730 685 360 106
RsrII CG'GwC_CG
Cuts at: 3327 3327
Size: 5132
SacI G_AGCT'C
Cuts at: 1415 2146 1415
Size: 731 4401
SalI G'TCGA_C
Cuts at: 2169 2169
Size: 5132
SanDI GG'GwC_CC
Cuts at: 950 950
Size: 5132
SapI GCTCTTCn'nnn_
Cuts at: 3155 3365 3155
Size: 210 4922
SbfI CC_TGCA'GG
Cuts at: 752 774 2179 752
Size: 22 1405 3705
Fragments arranged by size:
3705 1405 22
ScaI AGT'ACT
Cuts at: 2020 4649 2020
Size: 2629 2503
SfcI C'TryA_G
Cuts at: 33 139 710 748 770 1621 1665 1884 2175
Size: 106 571 38 22 851 44 219 291
Cuts at: 2175 2860 3853 4044 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 851 685 571 291 219 191
106 44 38 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 517 517
Size: 5132
SfoI GGC'GCC
Cuts at: 1986 2564 2812 1986
Size: 578 248 4306
Fragments arranged by size:
4306 578 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5132
SmaI CCC'GGG
Cuts at: 1942 2154 1942
Size: 212 4920
SmlI C'TyrA_G
Cuts at: 565 1789 1861 2287 3202 3694 3956 565
Size: 1224 72 426 915 492 262 1741
Fragments arranged by size:
1741 1224 915 492 426 262 72
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5132
SphI G_CATG'C
Cuts at: 1152 2185 2685 1152
Size: 1033 500 3599
Fragments arranged by size:
3599 1033 500
StuI AGG'CCT
Cuts at: 487 487
Size: 5132
StyI C'CwwG_G
Cuts at: 107 389 515 644 704 1202 2266 2620 107
Size: 282 126 129 60 498 1064 354 2619
Fragments arranged by size:
2619 1064 498 354 282 129 126 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1088 2715 2848 3307 3516 1088
Size: 1627 133 459 209 2704
Fragments arranged by size:
2704 1627 459 209 133
TatI w'GTAC_w
Cuts at: 2018 4647 2018
Size: 2629 2503
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 442 474 1921 1953 4649 4681 442
Size: 32 1447 32 2696 32 893
Fragments arranged by size:
2696 1447 893 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2929 2929
Size: 5132
XbaI T'CTAG_A
Cuts at: 2163 2163
Size: 5132
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 711 874 1054 2627 711
Size: 163 180 1573 3216
Fragments arranged by size:
3216 1573 180 163
XmaI C'CCGG_G
Cuts at: 1940 2152 1940
Size: 212 4920
XmnI GAAnn'nnTTC
Cuts at: 574 1426 1600 574
Size: 852 174 4106
Fragments arranged by size:
4106 852 174
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BmgBI BmtI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PflMI PshAI
PsiI PspXI PsrI SacII SexAI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI